Rroxscaffold_7G00217300

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000686
Physical Location & Seq
Forward (+)
67777872 .. 67784119
6248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00217300.1

Sequence Viewer

Length: 804 bp
ATGTTTTTTGATATTGATGGGCGTGGCAAAGACATTTTTCCCACAGTTTATGCCCTCTGGAAGGTCCCTGATCTCTTGCCTTCTTTCATGCTGAAAGGGGGCGAGGTTTCTCGATATGTTATTGGAGGGGCAGATTTGATGTTTCCCGGTATCAGCATACCTGCTGAAGGTTTACCATCGTTTATAGCTGGGGAAGCATGGGCGGTTAAAGTTCCAGGAAATCCAGCTTCCATTGCTGTGGGAACTACTACTATGAGTTGCACTGAAGCATTAAAAGCTGGATTGCGCGGAAAAGCCTTGAAAATTGCTCACTATTATGGTGACTTACTTTGGGAATCAGTTGAGGGCCCTTATGTGCCAAATGCAGGTTTCTTAGAAGATGCTGTTTTTGAGGATCCTACCTTGTCATCAGCTCAAATTTCTGATTCATATAATGGTGCAAATGATGGATCACATGATCAACTAAATGGCATTGATGATAAGGAAATGGGGCAACATGTTGATGTTGCTGATGTGCAGTCTGAACCAAGTTCTGCTTCGGTTGCACTGATTGATGCTAGGAATGAGATTGCTGAGGAAATAACTGCAAATGTAGATGATCTGAAGCTAACAGATGATGATTCTGCTAATCAGTCGACTGGGAGGATCAACATCCTCTTTCTGCCGAGGATGTGGACATGCTTTTGGAAAAATGCCTTTTGCAAGCATTGCATACAACTGTCAAAGACAAAGACCTTCCCATGCCCGGAAGCACTCTATGGTCAAACTATGTTTTACCTTGTAGGCCTTCAGGCATCACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SUI1

Protein Analysis

267

Amino Acids

28.95

Weight (kDa)

4.56

Isoelectric Point (pI)

37.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUA_elF2D PF26292 34 - 108 2.3e-21 elF2D, PUA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000686)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71350
fragaria_vesca FvH4_2g00430 FvH4_2g00430 FvH4_2g00430 FvH4_2g00430
malus_domestica MD10G1002600.v1.1
prunus_persica Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0249251 RchiOBHm_Chr6g0249261 RchiOBHm_Chr6g0249701 RchiOBHm_Chr6g0249761 RchiOBHm_Chr6g0249851 RchiOBHm_Chr6g0249861 RchiOBHm_Chr6g0249871 RchiOBHm_Chr6g0249891 RchiOBHm_Chr6g0249901 RchiOBHm_Chr6g0249911 RchiOBHm_Chr6g0249981 RchiOBHm_Chr6g0249991 RchiOBHm_Chr6g0250001 RchiOBHm_Chr6g0250151 RchiOBHm_Chr6g0250171 RchiOBHm_Chr6g0250191 RchiOBHm_Chr6g0250211 RchiOBHm_Chr6g0250221 RchiOBHm_Chr6g0250321 RchiOBHm_Chr6g0250391
rosa_laevigata RLG00000015543 RLG00000015548
rosa_multiflora Rmu_sc0000411.1_g000004 Rmu_sc0000411.1_g000009 Rmu_sc0000704.1_g000007 Rmu_sc0000704.1_g000011 Rmu_sc0001209.1_g000038 Rmu_sc0001209.1_g000042 Rmu_sc0001209.1_g000048 Rmu_sc0003293.1_g000005 Rmu_sc0004691.1_g000005 Rmu_sc0004691.1_g000007 Rmu_sc0004691.1_g000009 Rmu_sc0006032.1_g000001 Rmu_sc0006032.1_g000004 Rmu_sc0006032.1_g000005 Rmu_sc0008209.1_g000003 Rmu_sc0008209.1_g000010 Rmu_sc0009002.1_g000001 Rmu_sc0009002.1_g000005 Rmu_sc0009002.1_g000009
rosa_roxburghii Rroxscaffold_3G00218110 Rroxscaffold_7G00217270 Rroxscaffold_7G00217290 Rroxscaffold_7G00217300 Rroxscaffold_7G00217340
rosa_rugosa Rorug05G0494700 Rorug05G0495000 Rorug05G0496000 Rorug05G0498100 Rorug05G0498200
rosa_samantha Rh6AG000200 Rh6AG000300 Rh6AG004700 Rh6AG004800 Rh6AG005200 Rh6AG005300 Rh6AG005600 Rh6BG005500 Rh6BG005600 Rh6CG003700 Rh6DG003600
rosa_wichuraiana Rw6G000560 Rw6G000600 Rw6G000620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 169, 356
AccI GTMKAC 1 cut(s) 635
AccII CGCG 1 cut(s) 288
AciI CCGC 2 cut(s) 203, 288
AclWI GGATC 4 cut(s) 389, 402, 457, 653
AcsI RAATTY 1 cut(s) 417
AcuI CTGAAG 4 cut(s) 186, 285, 623, 773
AfiI CCNNNNNNNGG 4 cut(s) 61, 167, 365, 745
AflIII ACRYGT 1 cut(s) 496
AgsI TTSAA 1 cut(s) 301
AjnI CCWGG 1 cut(s) 214
AjuI GAANNNNNNNTTGG 2 cut(s) 520, 552
AluBI AGCT 5 cut(s) 188, 227, 278, 413, 607
AluI AGCT 5 cut(s) 188, 227, 278, 413, 607
AlwI GGATC 4 cut(s) 389, 402, 457, 653
AoxI GGCC 2 cut(s) 346, 784
ApaI GGGCCC 1 cut(s) 350
ApoI RAATTY 1 cut(s) 417
AspLEI GCGC 1 cut(s) 288
AspS9I GGNCC 3 cut(s) 64, 346, 347
AsuC2I CCSGG 2 cut(s) 147, 746
AsuHPI GGTGA 1 cut(s) 332
AvaII GGWCC 1 cut(s) 64
BaeGI GKGCMC 1 cut(s) 350
BamHI GGATCC 1 cut(s) 394
BanII GRGCYC 1 cut(s) 350
BbvCI CCTCAGC 1 cut(s) 573
BccI CCATC 3 cut(s) 11, 184, 440
BciT130I CCWGG 1 cut(s) 216
BclI TGATCA 1 cut(s) 457
BcnI CCSGG 2 cut(s) 147, 746
BfaI CTAG 2 cut(s) 558, 802
BfuAI ACCTGC 2 cut(s) 169, 356
Bme1390I CCNGG 3 cut(s) 147, 216, 746
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 3 cut(s) 64, 346, 347
BmiI GGNNCC 3 cut(s) 66, 348, 396
BmrFI CCNGG 3 cut(s) 147, 216, 746
BmrI ACTGGG 1 cut(s) 648
BmsI GCATC 2 cut(s) 370, 544
BmuI ACTGGG 1 cut(s) 648
Bpu10I CCTNAGC 1 cut(s) 573
BpuMI CCSGG 2 cut(s) 147, 746
BsaBI GATNNNNATC 1 cut(s) 650
BsaJI CCNNGG 1 cut(s) 665
Bsc4I CCNNNNNNNGG 4 cut(s) 61, 167, 365, 745
Bse1I ACTGG 1 cut(s) 643
Bse3DI GCAATG 2 cut(s) 231, 706
Bse8I GATNNNNATC 1 cut(s) 650
BseBI CCWGG 1 cut(s) 216
BseDI CCNNGG 1 cut(s) 665
BseGI GGATG 2 cut(s) 651, 675
BseJI GATNNNNATC 1 cut(s) 650
BseLI CCNNNNNNNGG 4 cut(s) 61, 167, 365, 745
BseMI GCAATG 2 cut(s) 231, 706
BseMII CTCAG 1 cut(s) 564
BseNI ACTGG 1 cut(s) 643
BseSI GKGCMC 1 cut(s) 350
BseYI CCCAGC 1 cut(s) 188
BsgI GTGCAG 1 cut(s) 536
Bsh1236I CGCG 1 cut(s) 288
BshFI GGCC 2 cut(s) 348, 786
BsiSI CCGG 2 cut(s) 147, 746
BslFI GGGAC 1 cut(s) 50
BslI CCNNNNNNNGG 4 cut(s) 61, 167, 365, 745
BsmFI GGGAC 1 cut(s) 50
BsnI GGCC 2 cut(s) 348, 786
Bsp120I GGGCCC 1 cut(s) 346
Bsp1286I GDGCHC 1 cut(s) 350
Bsp143I GATC 6 cut(s) 70, 394, 449, 457, 598, 645
BspACI CCGC 2 cut(s) 203, 288
BspANI GGCC 2 cut(s) 348, 786
BspCNI CTCAG 1 cut(s) 565
BspFNI CGCG 1 cut(s) 288
BspLI GGNNCC 3 cut(s) 66, 348, 396
BspMI ACCTGC 2 cut(s) 169, 356
BspPI GGATC 4 cut(s) 389, 402, 457, 653
BsrDI GCAATG 2 cut(s) 231, 706
BsrI ACTGG 1 cut(s) 643
BssECI CCNNGG 1 cut(s) 665
BssMI GATC 6 cut(s) 70, 394, 449, 457, 598, 645
Bst2UI CCWGG 1 cut(s) 216
Bst4CI ACNGT 2 cut(s) 46, 720
BstAPI GCANNNNNTGC 1 cut(s) 708
BstC8I GCNNGC 1 cut(s) 704
BstDEI CTNAG 2 cut(s) 373, 573
BstENI CCTNNNNNAGG 2 cut(s) 59, 165
BstF5I GGATG 2 cut(s) 651, 675
BstFNI CGCG 1 cut(s) 288
BstHHI GCGC 1 cut(s) 288
BstKTI GATC 6 cut(s) 73, 397, 452, 460, 601, 648
BstMBI GATC 6 cut(s) 70, 394, 449, 457, 598, 645
BstMWI GCNNNNNNNGC 5 cut(s) 194, 233, 275, 542, 708
BstNI CCWGG 1 cut(s) 216
BstNSI RCATGY 2 cut(s) 500, 681
BstSCI CCNGG 3 cut(s) 145, 214, 744
BstSLI GKGCMC 1 cut(s) 350
BstUI CGCG 1 cut(s) 288
BstX2I RGATCY 1 cut(s) 394
BstXI CCANNNNNNTGG 1 cut(s) 238
BstYI RGATCY 1 cut(s) 394
BsuRI GGCC 2 cut(s) 348, 786
BtsCI GGATG 2 cut(s) 651, 675
BtsIMutI CAGTG 2 cut(s) 261, 545
BveI ACCTGC 2 cut(s) 169, 356
Cac8I GCNNGC 1 cut(s) 704
CfoI GCGC 1 cut(s) 288
Cfr13I GGNCC 3 cut(s) 64, 346, 347
CviAII CATG 6 cut(s) 88, 198, 455, 497, 678, 741
CviJI RGCY 8 cut(s) 188, 227, 278, 296, 348, 413, 607, 786
CviKI_1 RGCY 8 cut(s) 188, 227, 278, 296, 348, 413, 607, 786
DdeI CTNAG 2 cut(s) 373, 573
DpnI GATC 6 cut(s) 72, 396, 451, 459, 600, 647
DpnII GATC 6 cut(s) 70, 394, 449, 457, 598, 645
Eco147I AGGCCT 1 cut(s) 786
Eco24I GRGCYC 1 cut(s) 350
Eco47I GGWCC 1 cut(s) 64
Eco57I CTGAAG 4 cut(s) 186, 285, 623, 773
EcoNI CCTNNNNNAGG 2 cut(s) 59, 165
EcoO109I RGGNCCY 3 cut(s) 64, 346, 347
EcoRII CCWGG 1 cut(s) 214
EcoT38I GRGCYC 1 cut(s) 350
FaeI CATG 6 cut(s) 91, 201, 458, 500, 681, 744
FalI AAGNNNNNCTT 2 cut(s) 520, 552
FaqI GGGAC 1 cut(s) 50
FatI CATG 6 cut(s) 87, 197, 454, 496, 677, 740
FbaI TGATCA 1 cut(s) 457
FblI GTMKAC 1 cut(s) 635
FokI GGATG 2 cut(s) 638, 682
FriOI GRGCYC 1 cut(s) 350
FspBI CTAG 2 cut(s) 558, 802
GlaI GCGC 1 cut(s) 287
GsaI CCCAGC 1 cut(s) 192
HaeIII GGCC 2 cut(s) 348, 786
HapII CCGG 2 cut(s) 147, 746
HhaI GCGC 1 cut(s) 288
Hin1II CATG 6 cut(s) 91, 201, 458, 500, 681, 744
Hin6I GCGC 1 cut(s) 286
HinP1I GCGC 1 cut(s) 286
HincII GTYRAC 1 cut(s) 636
HindII GTYRAC 1 cut(s) 636
HinfI GANTC 3 cut(s) 335, 425, 620
HpaII CCGG 2 cut(s) 147, 746
HphI GGTGA 1 cut(s) 332
Hpy166II GTNNAC 3 cut(s) 173, 636, 675
Hpy188I TCNGA 3 cut(s) 424, 523, 603
Hpy188III TCNNGA 2 cut(s) 58, 111
Hpy8I GTNNAC 3 cut(s) 173, 636, 675
HpyAV CCTTC 5 cut(s) 55, 90, 161, 745, 797
HpyCH4III ACNGT 2 cut(s) 46, 720
HpyCH4V TGCA 8 cut(s) 261, 365, 440, 517, 545, 587, 702, 711
HpyF10VI GCNNNNNNNGC 5 cut(s) 194, 233, 275, 542, 708
HpyF3I CTNAG 2 cut(s) 373, 573
Hsp92II CATG 6 cut(s) 91, 201, 458, 500, 681, 744
HspAI GCGC 1 cut(s) 286
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 6 cut(s) 70, 394, 449, 457, 598, 645
LweI GCATC 2 cut(s) 370, 544
MaeI CTAG 2 cut(s) 558, 802
MaeIII GTNAC 1 cut(s) 320
MalI GATC 6 cut(s) 72, 396, 451, 459, 600, 647
MboI GATC 6 cut(s) 70, 394, 449, 457, 598, 645
MboII GAAGA 1 cut(s) 389
MflI RGATCY 1 cut(s) 394
MhlI GDGCHC 1 cut(s) 350
MluCI AATT 2 cut(s) 303, 417
MnlI CCTC 9 cut(s) 65, 97, 119, 337, 385, 568, 636, 660, 665
MseI TTAA 2 cut(s) 207, 272
MslI CAYNNNNRTG 3 cut(s) 236, 315, 501
MspI CCGG 2 cut(s) 147, 746
MspR9I CCNGG 3 cut(s) 147, 216, 746
MvaI CCWGG 1 cut(s) 216
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 5 cut(s) 194, 233, 275, 542, 708
NciI CCSGG 2 cut(s) 147, 746
NdeII GATC 6 cut(s) 70, 394, 449, 457, 598, 645
NlaIII CATG 6 cut(s) 91, 201, 458, 500, 681, 744
NlaIV GGNNCC 3 cut(s) 66, 348, 396
NmeAIII GCCGAG 1 cut(s) 690
NmuCI GTSAC 1 cut(s) 320
NspI RCATGY 2 cut(s) 500, 681
PceI AGGCCT 1 cut(s) 786
PciI ACATGT 1 cut(s) 496
PfeI GAWTC 3 cut(s) 335, 425, 620
PfoI TCCNGGA 1 cut(s) 214
PpuMI RGGWCCY 1 cut(s) 64
PscI ACATGT 1 cut(s) 496
Psp5II RGGWCCY 1 cut(s) 64
Psp6I CCWGG 1 cut(s) 214
PspFI CCCAGC 1 cut(s) 188
PspGI CCWGG 1 cut(s) 214
PspN4I GGNNCC 3 cut(s) 66, 348, 396
PspOMI GGGCCC 1 cut(s) 346
PspPI GGNCC 3 cut(s) 64, 346, 347
PspPPI RGGWCCY 1 cut(s) 64
PsuI RGATCY 1 cut(s) 394
RseI CAYNNNNRTG 3 cut(s) 236, 315, 501
SalI GTCGAC 1 cut(s) 634
SaqAI TTAA 2 cut(s) 207, 272
Sau3AI GATC 6 cut(s) 70, 394, 449, 457, 598, 645
Sau96I GGNCC 3 cut(s) 64, 346, 347
ScrFI CCNGG 3 cut(s) 147, 216, 746
SduI GDGCHC 1 cut(s) 350
SfaNI GCATC 2 cut(s) 370, 544
SinI GGWCC 1 cut(s) 64
SmiMI CAYNNNNRTG 3 cut(s) 236, 315, 501
Sse9I AATT 2 cut(s) 303, 417
SseBI AGGCCT 1 cut(s) 786
SsiI CCGC 2 cut(s) 203, 288
SspMI CTAG 2 cut(s) 558, 802
StuI AGGCCT 1 cut(s) 786
StyD4I CCNGG 3 cut(s) 145, 214, 744
TaaI ACNGT 2 cut(s) 46, 720
TaqI TCGA 2 cut(s) 112, 635
TasI AATT 2 cut(s) 303, 417
TfiI GAWTC 3 cut(s) 335, 425, 620
Tru1I TTAA 2 cut(s) 207, 272
Tru9I TTAA 2 cut(s) 207, 272
TscAI CASTG 2 cut(s) 268, 552
TseFI GTSAC 1 cut(s) 320
Tsp45I GTSAC 1 cut(s) 320
TspDTI ATGAA 2 cut(s) 76, 417
TspRI CASTG 2 cut(s) 268, 552
VpaK11BI GGWCC 1 cut(s) 64
XagI CCTNNNNNAGG 2 cut(s) 59, 165
XapI RAATTY 1 cut(s) 417
XceI RCATGY 2 cut(s) 500, 681
XmiI GTMKAC 1 cut(s) 635
XspI CTAG 2 cut(s) 558, 802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.