eukaryotic translation initiation factor
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 88 |
| AclWI | GGATC | 4 cut(s) | 137, 150, 283, 296 |
| AcuI | CTGAAG | 1 cut(s) | 168 |
| AdeI | CACNNNGTG | 1 cut(s) | 19 |
| AfaI | GTAC | 1 cut(s) | 190 |
| AgsI | TTSAA | 3 cut(s) | 61, 239, 278 |
| AjnI | CCWGG | 1 cut(s) | 323 |
| AluBI | AGCT | 2 cut(s) | 152, 166 |
| AluI | AGCT | 2 cut(s) | 152, 166 |
| AlwI | GGATC | 4 cut(s) | 137, 150, 283, 296 |
| ApeKI | GCWGC | 1 cut(s) | 166 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 176 |
| BamHI | GGATCC | 2 cut(s) | 142, 288 |
| BbvI | GCAGC | 1 cut(s) | 153 |
| BccI | CCATC | 1 cut(s) | 18 |
| BciT130I | CCWGG | 1 cut(s) | 325 |
| BfmI | CTRYAG | 1 cut(s) | 167 |
| BisI | GCNGC | 2 cut(s) | 89, 167 |
| BlsI | GCNGC | 2 cut(s) | 90, 168 |
| Bme1390I | CCNGG | 1 cut(s) | 325 |
| BmiI | GGNNCC | 2 cut(s) | 144, 290 |
| BmrFI | CCNGG | 1 cut(s) | 325 |
| BmsI | GCATC | 1 cut(s) | 167 |
| Bse1I | ACTGG | 1 cut(s) | 129 |
| BseBI | CCWGG | 1 cut(s) | 325 |
| BseNI | ACTGG | 1 cut(s) | 129 |
| BseXI | GCAGC | 1 cut(s) | 153 |
| Bsp143I | GATC | 2 cut(s) | 142, 288 |
| BspACI | CCGC | 1 cut(s) | 88 |
| BspLI | GGNNCC | 2 cut(s) | 144, 290 |
| BspMAI | CTGCAG | 1 cut(s) | 171 |
| BspPI | GGATC | 4 cut(s) | 137, 150, 283, 296 |
| BsrI | ACTGG | 1 cut(s) | 129 |
| BssMI | GATC | 2 cut(s) | 142, 288 |
| Bst2UI | CCWGG | 1 cut(s) | 325 |
| Bst4CI | ACNGT | 2 cut(s) | 67, 196 |
| BstC8I | GCNNGC | 2 cut(s) | 6, 184 |
| BstKTI | GATC | 2 cut(s) | 145, 291 |
| BstMBI | GATC | 2 cut(s) | 142, 288 |
| BstNI | CCWGG | 1 cut(s) | 325 |
| BstSCI | CCNGG | 1 cut(s) | 323 |
| BstSFI | CTRYAG | 1 cut(s) | 167 |
| BstV1I | GCAGC | 1 cut(s) | 153 |
| BstX2I | RGATCY | 2 cut(s) | 142, 288 |
| BstYI | RGATCY | 2 cut(s) | 142, 288 |
| Cac8I | GCNNGC | 2 cut(s) | 6, 184 |
| Csp6I | GTAC | 1 cut(s) | 189 |
| CviAII | CATG | 1 cut(s) | 210 |
| CviJI | RGCY | 3 cut(s) | 8, 152, 166 |
| CviKI_1 | RGCY | 3 cut(s) | 8, 152, 166 |
| CviQI | GTAC | 1 cut(s) | 189 |
| DpnI | GATC | 2 cut(s) | 144, 290 |
| DpnII | GATC | 2 cut(s) | 142, 288 |
| DraIII | CACNNNGTG | 1 cut(s) | 19 |
| Eco57I | CTGAAG | 1 cut(s) | 168 |
| EcoRII | CCWGG | 1 cut(s) | 323 |
| FaeI | CATG | 1 cut(s) | 213 |
| FaiI | YATR | 2 cut(s) | 211, 285 |
| FatI | CATG | 1 cut(s) | 209 |
| Fnu4HI | GCNGC | 2 cut(s) | 89, 167 |
| Fsp4HI | GCNGC | 2 cut(s) | 89, 167 |
| GluI | GCNGC | 2 cut(s) | 89, 167 |
| Hin1II | CATG | 1 cut(s) | 213 |
| HindIII | AAGCTT | 1 cut(s) | 150 |
| HinfI | GANTC | 2 cut(s) | 35, 256 |
| Hpy188I | TCNGA | 1 cut(s) | 163 |
| Hpy188III | TCNNGA | 4 cut(s) | 70, 120, 146, 314 |
| HpyAV | CCTTC | 1 cut(s) | 211 |
| HpyCH4III | ACNGT | 2 cut(s) | 67, 196 |
| HpyCH4V | TGCA | 3 cut(s) | 4, 169, 186 |
| Hsp92II | CATG | 1 cut(s) | 213 |
| Kzo9I | GATC | 2 cut(s) | 142, 288 |
| LpnPI | CCDG | 8 cut(s) | 83, 105, 110, 159, 209, 218, 245, 310 |
| Lsp1109I | GCAGC | 1 cut(s) | 153 |
| LweI | GCATC | 1 cut(s) | 167 |
| MaeIII | GTNAC | 1 cut(s) | 19 |
| MalI | GATC | 2 cut(s) | 144, 290 |
| MboI | GATC | 2 cut(s) | 142, 288 |
| MboII | GAAGA | 1 cut(s) | 184 |
| MflI | RGATCY | 2 cut(s) | 142, 288 |
| MlyI | GAGTC | 1 cut(s) | 29 |
| MnlI | CCTC | 2 cut(s) | 220, 301 |
| MroXI | GAANNNNTTC | 1 cut(s) | 176 |
| MspR9I | CCNGG | 1 cut(s) | 325 |
| MvaI | CCWGG | 1 cut(s) | 325 |
| NdeII | GATC | 2 cut(s) | 142, 288 |
| NlaIII | CATG | 1 cut(s) | 213 |
| NlaIV | GGNNCC | 2 cut(s) | 144, 290 |
| NmuCI | GTSAC | 1 cut(s) | 19 |
| PdmI | GAANNNNTTC | 1 cut(s) | 176 |
| PfeI | GAWTC | 1 cut(s) | 256 |
| PkrI | GCNGC | 2 cut(s) | 90, 168 |
| PleI | GAGTC | 1 cut(s) | 29 |
| PpsI | GAGTC | 1 cut(s) | 29 |
| Psp6I | CCWGG | 1 cut(s) | 323 |
| PspGI | CCWGG | 1 cut(s) | 323 |
| PspN4I | GGNNCC | 2 cut(s) | 144, 290 |
| PstI | CTGCAG | 1 cut(s) | 171 |
| PsuI | RGATCY | 2 cut(s) | 142, 288 |
| RsaI | GTAC | 1 cut(s) | 190 |
| RsaNI | GTAC | 1 cut(s) | 189 |
| SatI | GCNGC | 2 cut(s) | 89, 167 |
| Sau3AI | GATC | 2 cut(s) | 142, 288 |
| SchI | GAGTC | 1 cut(s) | 29 |
| ScrFI | CCNGG | 1 cut(s) | 325 |
| SetI | ASST | 7 cut(s) | 55, 97, 154, 168, 231, 244, 312 |
| SfaNI | GCATC | 1 cut(s) | 167 |
| SfcI | CTRYAG | 1 cut(s) | 167 |
| SsiI | CCGC | 1 cut(s) | 88 |
| StyD4I | CCNGG | 1 cut(s) | 323 |
| TaaI | ACNGT | 2 cut(s) | 67, 196 |
| TatI | WGTACW | 1 cut(s) | 188 |
| TauI | GCSGC | 1 cut(s) | 91 |
| TfiI | GAWTC | 1 cut(s) | 256 |
| TseFI | GTSAC | 1 cut(s) | 19 |
| TseI | GCWGC | 1 cut(s) | 166 |
| Tsp45I | GTSAC | 1 cut(s) | 19 |
| XmnI | GAANNNNTTC | 1 cut(s) | 176 |