RchiOBHm_Chr6g0249901

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000686
Physical Location & Seq
Reverse (-)
5669894 .. 5672278
2385 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22402

Sequence Viewer

Length: 666 bp
ATGAGTTGCACTGAAGCATTAAAAGCTGGATTGCGTGGAAAAGCCTTAAAAATTGCTCACTATTATGGTGACTTACTTTGGGAATCAGTTGAGGGCCCTTATGTGCCAAATGCAGGTTTCTTAGAAGATGCTGTTTTTGAGGATCCTACCTTGTCATCAGCTCAAATTTCTGATTCATATGATGGTGCAAATGATGGATCACATGATCAACTGAATGGCATTGATGATAAGGAAATGGGGCAACATGTTGACGTTGCTGATGTGCAGTCTGAACAAAGTTCTGCTTCAGTTGCACCGATTGATGTTAGGAATGAGATTGCTGAGGAAGTAACTGCAAATGTAGGTGATCTGAAGCTAACAGATGATGATTCTGCTAATCAGTCGACTGGGGAGGATCAACATCATCTTTCTGCCAAGGATGTGGACATGCTTTTGGAAAAATGCCTTTTGCAAGCATTGCATACAACTGTCAAAGACAAAGACCTTCCCATGCCCGGAAGCACTCTATGGTCAAACTATGTTTTACCTTGTAGGCCTTCAGGCATCACACTAGACATCAAGAAATCATCACACAAGAAATTATCAAAGTGGTTACAAGCTAAATGTTCTACAGGATTGATTAGAGTGAAGGAAGATAAATATAAGGAGTCTATTACTTTCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SUI1

Protein Analysis

221

Amino Acids

24.22

Weight (kDa)

4.76

Isoelectric Point (pI)

44.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_eIF2D PF25304 142 - 219 3.2e-29 eIF2D winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000686)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71350
fragaria_vesca FvH4_2g00430 FvH4_2g00430 FvH4_2g00430 FvH4_2g00430
malus_domestica MD10G1002600.v1.1
prunus_persica Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0249251 RchiOBHm_Chr6g0249261 RchiOBHm_Chr6g0249701 RchiOBHm_Chr6g0249761 RchiOBHm_Chr6g0249851 RchiOBHm_Chr6g0249861 RchiOBHm_Chr6g0249871 RchiOBHm_Chr6g0249891 RchiOBHm_Chr6g0249901 RchiOBHm_Chr6g0249911 RchiOBHm_Chr6g0249981 RchiOBHm_Chr6g0249991 RchiOBHm_Chr6g0250001 RchiOBHm_Chr6g0250151 RchiOBHm_Chr6g0250171 RchiOBHm_Chr6g0250191 RchiOBHm_Chr6g0250211 RchiOBHm_Chr6g0250221 RchiOBHm_Chr6g0250321 RchiOBHm_Chr6g0250391
rosa_laevigata RLG00000015543 RLG00000015548
rosa_multiflora Rmu_sc0000411.1_g000004 Rmu_sc0000411.1_g000009 Rmu_sc0000704.1_g000007 Rmu_sc0000704.1_g000011 Rmu_sc0001209.1_g000038 Rmu_sc0001209.1_g000042 Rmu_sc0001209.1_g000048 Rmu_sc0003293.1_g000005 Rmu_sc0004691.1_g000005 Rmu_sc0004691.1_g000007 Rmu_sc0004691.1_g000009 Rmu_sc0006032.1_g000001 Rmu_sc0006032.1_g000004 Rmu_sc0006032.1_g000005 Rmu_sc0008209.1_g000003 Rmu_sc0008209.1_g000010 Rmu_sc0009002.1_g000001 Rmu_sc0009002.1_g000005 Rmu_sc0009002.1_g000009
rosa_roxburghii Rroxscaffold_3G00218110 Rroxscaffold_7G00217270 Rroxscaffold_7G00217290 Rroxscaffold_7G00217300 Rroxscaffold_7G00217340
rosa_rugosa Rorug05G0494700 Rorug05G0495000 Rorug05G0496000 Rorug05G0498100 Rorug05G0498200
rosa_samantha Rh6AG000200 Rh6AG000300 Rh6AG004700 Rh6AG004800 Rh6AG005200 Rh6AG005300 Rh6AG005600 Rh6BG005500 Rh6BG005600 Rh6CG003700 Rh6DG003600
rosa_wichuraiana Rw6G000560 Rw6G000600 Rw6G000620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 104
AccI GTMKAC 1 cut(s) 383
AclWI GGATC 4 cut(s) 137, 150, 205, 402
AcsI RAATTY 1 cut(s) 165
AcuI CTGAAG 4 cut(s) 33, 270, 371, 522
AfiI CCNNNNNNNGG 2 cut(s) 113, 494
AflIII ACRYGT 1 cut(s) 244
AluBI AGCT 4 cut(s) 26, 161, 355, 599
AluI AGCT 4 cut(s) 26, 161, 355, 599
AlwI GGATC 4 cut(s) 137, 150, 205, 402
AoxI GGCC 2 cut(s) 94, 533
ApaI GGGCCC 1 cut(s) 98
ApoI RAATTY 1 cut(s) 165
AspS9I GGNCC 2 cut(s) 94, 95
AsuC2I CCSGG 1 cut(s) 495
AsuHPI GGTGA 2 cut(s) 80, 356
BaeGI GKGCMC 1 cut(s) 98
BamHI GGATCC 1 cut(s) 142
BanII GRGCYC 1 cut(s) 98
BbvCI CCTCAGC 1 cut(s) 321
BccI CCATC 2 cut(s) 176, 188
BclI TGATCA 1 cut(s) 205
BcnI CCSGG 1 cut(s) 495
BfaI CTAG 1 cut(s) 551
BfmI CTRYAG 1 cut(s) 609
BfuAI ACCTGC 1 cut(s) 104
Bme1390I CCNGG 1 cut(s) 495
BmgT120I GGNCC 2 cut(s) 94, 95
BmiI GGNNCC 2 cut(s) 96, 144
BmrFI CCNGG 1 cut(s) 495
BmrI ACTGGG 1 cut(s) 396
BmsI GCATC 2 cut(s) 118, 552
BmuI ACTGGG 1 cut(s) 396
Bpu10I CCTNAGC 1 cut(s) 321
BpuMI CCSGG 1 cut(s) 495
BsaBI GATNNNNATC 1 cut(s) 399
BsaJI CCNNGG 1 cut(s) 414
Bsc4I CCNNNNNNNGG 2 cut(s) 113, 494
Bse1I ACTGG 1 cut(s) 391
Bse3DI GCAATG 1 cut(s) 455
Bse8I GATNNNNATC 1 cut(s) 399
BseDI CCNNGG 1 cut(s) 414
BseGI GGATG 1 cut(s) 424
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 2 cut(s) 113, 494
BseMI GCAATG 1 cut(s) 455
BseMII CTCAG 1 cut(s) 312
BseNI ACTGG 1 cut(s) 391
BseSI GKGCMC 1 cut(s) 98
BsgI GTGCAG 1 cut(s) 284
BshFI GGCC 2 cut(s) 96, 535
BsiSI CCGG 1 cut(s) 495
BslI CCNNNNNNNGG 2 cut(s) 113, 494
BsnI GGCC 2 cut(s) 96, 535
Bsp120I GGGCCC 1 cut(s) 94
Bsp1286I GDGCHC 1 cut(s) 98
Bsp143I GATC 5 cut(s) 142, 197, 205, 346, 394
BspANI GGCC 2 cut(s) 96, 535
BspCNI CTCAG 1 cut(s) 313
BspLI GGNNCC 2 cut(s) 96, 144
BspMI ACCTGC 1 cut(s) 104
BspPI GGATC 4 cut(s) 137, 150, 205, 402
BsrDI GCAATG 1 cut(s) 455
BsrI ACTGG 1 cut(s) 391
BssECI CCNNGG 1 cut(s) 414
BssMI GATC 5 cut(s) 142, 197, 205, 346, 394
BssT1I CCWWGG 1 cut(s) 414
Bst4CI ACNGT 1 cut(s) 469
BstAPI GCANNNNNTGC 1 cut(s) 457
BstC8I GCNNGC 1 cut(s) 453
BstDEI CTNAG 2 cut(s) 121, 321
BstF5I GGATG 1 cut(s) 424
BstKTI GATC 5 cut(s) 145, 200, 208, 349, 397
BstMBI GATC 5 cut(s) 142, 197, 205, 346, 394
BstMWI GCNNNNNNNGC 3 cut(s) 23, 290, 457
BstNSI RCATGY 2 cut(s) 248, 430
BstSCI CCNGG 1 cut(s) 493
BstSFI CTRYAG 1 cut(s) 609
BstSLI GKGCMC 1 cut(s) 98
BstX2I RGATCY 1 cut(s) 142
BstXI CCANNNNNNTGG 1 cut(s) 421
BstYI RGATCY 1 cut(s) 142
BsuRI GGCC 2 cut(s) 96, 535
BtsCI GGATG 1 cut(s) 424
BtsIMutI CAGTG 1 cut(s) 9
BveI ACCTGC 1 cut(s) 104
Cac8I GCNNGC 1 cut(s) 453
Cfr13I GGNCC 2 cut(s) 94, 95
CviAII CATG 4 cut(s) 203, 245, 427, 490
CviJI RGCY 7 cut(s) 26, 44, 96, 161, 355, 535, 599
CviKI_1 RGCY 7 cut(s) 26, 44, 96, 161, 355, 535, 599
DdeI CTNAG 2 cut(s) 121, 321
DpnI GATC 5 cut(s) 144, 199, 207, 348, 396
DpnII GATC 5 cut(s) 142, 197, 205, 346, 394
Eco130I CCWWGG 1 cut(s) 414
Eco147I AGGCCT 1 cut(s) 535
Eco24I GRGCYC 1 cut(s) 98
Eco57I CTGAAG 4 cut(s) 33, 270, 371, 522
EcoO109I RGGNCCY 2 cut(s) 94, 95
EcoT14I CCWWGG 1 cut(s) 414
EcoT38I GRGCYC 1 cut(s) 98
ErhI CCWWGG 1 cut(s) 414
FaeI CATG 4 cut(s) 206, 248, 430, 493
FalI AAGNNNNNCTT 2 cut(s) 268, 300
FatI CATG 4 cut(s) 202, 244, 426, 489
FauNDI CATATG 1 cut(s) 178
FbaI TGATCA 1 cut(s) 205
FblI GTMKAC 1 cut(s) 383
FokI GGATG 1 cut(s) 431
FriOI GRGCYC 1 cut(s) 98
FspBI CTAG 1 cut(s) 551
HaeIII GGCC 2 cut(s) 96, 535
HapII CCGG 1 cut(s) 495
Hin1II CATG 4 cut(s) 206, 248, 430, 493
HincII GTYRAC 2 cut(s) 250, 384
HindII GTYRAC 2 cut(s) 250, 384
HinfI GANTC 4 cut(s) 83, 173, 368, 647
HpaII CCGG 1 cut(s) 495
HphI GGTGA 2 cut(s) 80, 356
Hpy166II GTNNAC 3 cut(s) 250, 384, 424
Hpy188I TCNGA 3 cut(s) 172, 271, 351
Hpy188III TCNNGA 1 cut(s) 559
Hpy8I GTNNAC 3 cut(s) 250, 384, 424
HpyAV CCTTC 3 cut(s) 494, 546, 622
HpyCH4III ACNGT 1 cut(s) 469
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 8 cut(s) 9, 113, 188, 265, 293, 335, 451, 460
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 290, 457
HpyF3I CTNAG 2 cut(s) 121, 321
HpySE526I ACGT 1 cut(s) 252
Hsp92II CATG 4 cut(s) 206, 248, 430, 493
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 5 cut(s) 142, 197, 205, 346, 394
LpnPI CCDG 6 cut(s) 12, 99, 372, 508, 525, 597
LweI GCATC 2 cut(s) 118, 552
MaeI CTAG 1 cut(s) 551
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 3 cut(s) 68, 328, 591
MalI GATC 5 cut(s) 144, 199, 207, 348, 396
MboI GATC 5 cut(s) 142, 197, 205, 346, 394
MboII GAAGA 2 cut(s) 137, 644
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 1 cut(s) 98
MluCI AATT 3 cut(s) 51, 165, 578
MlyI GAGTC 1 cut(s) 656
MnlI CCTC 4 cut(s) 85, 133, 316, 385
MseI TTAA 2 cut(s) 20, 47
MslI CAYNNNNRTG 1 cut(s) 63
MspI CCGG 1 cut(s) 495
MspR9I CCNGG 1 cut(s) 495
MwoI GCNNNNNNNGC 3 cut(s) 23, 290, 457
NciI CCSGG 1 cut(s) 495
NdeI CATATG 1 cut(s) 178
NdeII GATC 5 cut(s) 142, 197, 205, 346, 394
NlaIII CATG 4 cut(s) 206, 248, 430, 493
NlaIV GGNNCC 2 cut(s) 96, 144
NmuCI GTSAC 1 cut(s) 68
NspI RCATGY 2 cut(s) 248, 430
PceI AGGCCT 1 cut(s) 535
PciI ACATGT 1 cut(s) 244
PfeI GAWTC 3 cut(s) 83, 173, 368
PleI GAGTC 1 cut(s) 655
PpsI GAGTC 1 cut(s) 655
PscI ACATGT 1 cut(s) 244
PspN4I GGNNCC 2 cut(s) 96, 144
PspOMI GGGCCC 1 cut(s) 94
PspPI GGNCC 2 cut(s) 94, 95
PsuI RGATCY 1 cut(s) 142
RseI CAYNNNNRTG 1 cut(s) 63
SalI GTCGAC 1 cut(s) 382
SaqAI TTAA 2 cut(s) 20, 47
Sau3AI GATC 5 cut(s) 142, 197, 205, 346, 394
Sau96I GGNCC 2 cut(s) 94, 95
SchI GAGTC 1 cut(s) 656
ScrFI CCNGG 1 cut(s) 495
SduI GDGCHC 1 cut(s) 98
SfaNI GCATC 2 cut(s) 118, 552
SfcI CTRYAG 1 cut(s) 609
SmiMI CAYNNNNRTG 1 cut(s) 63
Sse9I AATT 3 cut(s) 51, 165, 578
SseBI AGGCCT 1 cut(s) 535
SspMI CTAG 1 cut(s) 551
StuI AGGCCT 1 cut(s) 535
StyD4I CCNGG 1 cut(s) 493
StyI CCWWGG 1 cut(s) 414
TaaI ACNGT 1 cut(s) 469
TaiI ACGT 1 cut(s) 255
TaqI TCGA 1 cut(s) 383
TasI AATT 3 cut(s) 51, 165, 578
TfiI GAWTC 3 cut(s) 83, 173, 368
Tru1I TTAA 2 cut(s) 20, 47
Tru9I TTAA 2 cut(s) 20, 47
TscAI CASTG 1 cut(s) 16
TseFI GTSAC 1 cut(s) 68
Tsp45I GTSAC 1 cut(s) 68
TspDTI ATGAA 1 cut(s) 165
TspRI CASTG 1 cut(s) 16
XapI RAATTY 1 cut(s) 165
XceI RCATGY 2 cut(s) 248, 430
XmiI GTMKAC 1 cut(s) 383
XspI CTAG 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.