RchiOBHm_Chr6g0250001

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000686
Physical Location & Seq
Reverse (-)
5688241 .. 5689742
1502 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22411

Sequence Viewer

Length: 489 bp
ATGTGGGTCCGTATGATATATGAGGGGGATGTTGAGGAGCTTTGGGAAACATTATTGAAGCAGAGCAGTACCAAGCAAGGCCTTCAACACTACTATGATGATGATGATGCAGCATCTAGTAGTACTGGCAATACCAAGCGAGGCCGTCAACACTACTATGATAATGTTATTTATGCCCTCTGGAAGGTCCCTGATATATTGCCTTCTTTCATGCTGAAAGGGGGCGAGGTTTCTCGATATGTTATTGGAGGGGCGGATTTGATGTTTCCTGGTATCAGCATAGCTGCTGAAGGTCTACCATCATTTATAGCATGGGCGGTTAAAGTTCCAGGAAATCCAGCTCCCATTGCTGTGGGAACTACTACTATGAGCTGCACAGAAGCATTAAAAGCTGGGTTGCGTGGAAAAGCCTTAAAAATTGCTCACTATTATGGTGACTTACTTTGGGAATCAGTTGAGGGCCCTTATGTGCCAAATGCAGGTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SUI1

Protein Analysis

162

Amino Acids

17.81

Weight (kDa)

5.32

Isoelectric Point (pI)

56.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUA_elF2D PF26292 75 - 146 1.2e-18 elF2D, PUA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000686)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71350
fragaria_vesca FvH4_2g00430 FvH4_2g00430 FvH4_2g00430 FvH4_2g00430
malus_domestica MD10G1002600.v1.1
prunus_persica Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0249251 RchiOBHm_Chr6g0249261 RchiOBHm_Chr6g0249701 RchiOBHm_Chr6g0249761 RchiOBHm_Chr6g0249851 RchiOBHm_Chr6g0249861 RchiOBHm_Chr6g0249871 RchiOBHm_Chr6g0249891 RchiOBHm_Chr6g0249901 RchiOBHm_Chr6g0249911 RchiOBHm_Chr6g0249981 RchiOBHm_Chr6g0249991 RchiOBHm_Chr6g0250001 RchiOBHm_Chr6g0250151 RchiOBHm_Chr6g0250171 RchiOBHm_Chr6g0250191 RchiOBHm_Chr6g0250211 RchiOBHm_Chr6g0250221 RchiOBHm_Chr6g0250321 RchiOBHm_Chr6g0250391
rosa_laevigata RLG00000015543 RLG00000015548
rosa_multiflora Rmu_sc0000411.1_g000004 Rmu_sc0000411.1_g000009 Rmu_sc0000704.1_g000007 Rmu_sc0000704.1_g000011 Rmu_sc0001209.1_g000038 Rmu_sc0001209.1_g000042 Rmu_sc0001209.1_g000048 Rmu_sc0003293.1_g000005 Rmu_sc0004691.1_g000005 Rmu_sc0004691.1_g000007 Rmu_sc0004691.1_g000009 Rmu_sc0006032.1_g000001 Rmu_sc0006032.1_g000004 Rmu_sc0006032.1_g000005 Rmu_sc0008209.1_g000003 Rmu_sc0008209.1_g000010 Rmu_sc0009002.1_g000001 Rmu_sc0009002.1_g000005 Rmu_sc0009002.1_g000009
rosa_roxburghii Rroxscaffold_3G00218110 Rroxscaffold_7G00217270 Rroxscaffold_7G00217290 Rroxscaffold_7G00217300 Rroxscaffold_7G00217340
rosa_rugosa Rorug05G0494700 Rorug05G0495000 Rorug05G0496000 Rorug05G0498100 Rorug05G0498200
rosa_samantha Rh6AG000200 Rh6AG000300 Rh6AG004700 Rh6AG004800 Rh6AG005200 Rh6AG005300 Rh6AG005600 Rh6BG005500 Rh6BG005600 Rh6CG003700 Rh6DG003600
rosa_wichuraiana Rw6G000560 Rw6G000600 Rw6G000620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 470
AccI GTMKAC 1 cut(s) 295
AciI CCGC 2 cut(s) 254, 317
AcuI CTGAAG 1 cut(s) 309
AfaI GTAC 2 cut(s) 70, 124
AfiI CCNNNNNNNGG 2 cut(s) 184, 479
AgsI TTSAA 2 cut(s) 58, 86
AjnI CCWGG 2 cut(s) 268, 328
AluBI AGCT 5 cut(s) 40, 284, 341, 372, 392
AluI AGCT 5 cut(s) 40, 284, 341, 372, 392
AoxI GGCC 3 cut(s) 79, 142, 460
ApaI GGGCCC 1 cut(s) 464
ApeKI GCWGC 3 cut(s) 110, 284, 372
AspS9I GGNCC 4 cut(s) 7, 187, 460, 461
AsuHPI GGTGA 1 cut(s) 446
AvaII GGWCC 2 cut(s) 7, 187
BaeGI GKGCMC 1 cut(s) 464
BanII GRGCYC 1 cut(s) 464
BbvI GCAGC 3 cut(s) 122, 271, 359
BccI CCATC 1 cut(s) 307
BceAI ACGGC 1 cut(s) 129
BciT130I CCWGG 2 cut(s) 270, 330
BfaI CTAG 1 cut(s) 117
BfuAI ACCTGC 1 cut(s) 470
BisI GCNGC 3 cut(s) 111, 285, 373
BlsI GCNGC 3 cut(s) 112, 286, 374
BmcAI AGTACT 1 cut(s) 124
Bme1390I CCNGG 2 cut(s) 270, 330
Bme18I GGWCC 2 cut(s) 7, 187
BmgT120I GGNCC 4 cut(s) 7, 187, 460, 461
BmiI GGNNCC 3 cut(s) 8, 189, 462
BmrFI CCNGG 2 cut(s) 270, 330
BmsI GCATC 2 cut(s) 97, 122
Bsc4I CCNNNNNNNGG 2 cut(s) 184, 479
Bse1I ACTGG 1 cut(s) 130
Bse3DI GCAATG 1 cut(s) 345
BseBI CCWGG 2 cut(s) 270, 330
BseGI GGATG 1 cut(s) 34
BseLI CCNNNNNNNGG 2 cut(s) 184, 479
BseMI GCAATG 1 cut(s) 345
BseNI ACTGG 1 cut(s) 130
BseRI GAGGAG 1 cut(s) 50
BseSI GKGCMC 1 cut(s) 464
BseXI GCAGC 3 cut(s) 122, 271, 359
BseYI CCCAGC 1 cut(s) 392
BsgI GTGCAG 1 cut(s) 358
BshFI GGCC 3 cut(s) 81, 144, 462
BslFI GGGAC 1 cut(s) 173
BslI CCNNNNNNNGG 2 cut(s) 184, 479
BsmFI GGGAC 1 cut(s) 173
BsnI GGCC 3 cut(s) 81, 144, 462
Bsp120I GGGCCC 1 cut(s) 460
Bsp1286I GDGCHC 1 cut(s) 464
BspACI CCGC 2 cut(s) 254, 317
BspANI GGCC 3 cut(s) 81, 144, 462
BspLI GGNNCC 3 cut(s) 8, 189, 462
BspMI ACCTGC 1 cut(s) 470
BsrDI GCAATG 1 cut(s) 345
BsrI ACTGG 1 cut(s) 130
Bst2UI CCWGG 2 cut(s) 270, 330
BstENI CCTNNNNNAGG 1 cut(s) 182
BstF5I GGATG 1 cut(s) 34
BstMWI GCNNNNNNNGC 2 cut(s) 347, 389
BstNI CCWGG 2 cut(s) 270, 330
BstSCI CCNGG 2 cut(s) 268, 328
BstSLI GKGCMC 1 cut(s) 464
BstV1I GCAGC 3 cut(s) 122, 271, 359
BstXI CCANNNNNNTGG 1 cut(s) 352
BsuRI GGCC 3 cut(s) 81, 144, 462
BtsCI GGATG 1 cut(s) 34
BveI ACCTGC 1 cut(s) 470
Cfr13I GGNCC 4 cut(s) 7, 187, 460, 461
Csp6I GTAC 2 cut(s) 69, 123
CviAII CATG 2 cut(s) 211, 312
CviJI RGCY 9 cut(s) 40, 81, 144, 284, 341, 372, 392, 410, 462
CviKI_1 RGCY 9 cut(s) 40, 81, 144, 284, 341, 372, 392, 410, 462
CviQI GTAC 2 cut(s) 69, 123
EciI GGCGGA 1 cut(s) 269
Eco147I AGGCCT 1 cut(s) 81
Eco24I GRGCYC 1 cut(s) 464
Eco47I GGWCC 2 cut(s) 7, 187
Eco57I CTGAAG 1 cut(s) 309
EcoNI CCTNNNNNAGG 1 cut(s) 182
EcoO109I RGGNCCY 3 cut(s) 187, 460, 461
EcoRII CCWGG 2 cut(s) 268, 328
EcoT38I GRGCYC 1 cut(s) 464
FaeI CATG 2 cut(s) 214, 315
FaqI GGGAC 1 cut(s) 173
FatI CATG 2 cut(s) 210, 311
FblI GTMKAC 1 cut(s) 295
Fnu4HI GCNGC 3 cut(s) 111, 285, 373
FokI GGATG 1 cut(s) 41
FriOI GRGCYC 1 cut(s) 464
Fsp4HI GCNGC 3 cut(s) 111, 285, 373
FspBI CTAG 1 cut(s) 117
GluI GCNGC 3 cut(s) 111, 285, 373
GsaI CCCAGC 1 cut(s) 396
HaeIII GGCC 3 cut(s) 81, 144, 462
Hin1II CATG 2 cut(s) 214, 315
HincII GTYRAC 1 cut(s) 149
HindII GTYRAC 1 cut(s) 149
HinfI GANTC 1 cut(s) 449
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 2 cut(s) 149, 296
Hpy188I TCNGA 1 cut(s) 488
Hpy188III TCNNGA 2 cut(s) 181, 234
Hpy8I GTNNAC 2 cut(s) 149, 296
HpyAV CCTTC 4 cut(s) 92, 178, 213, 284
HpyCH4V TGCA 3 cut(s) 110, 375, 479
HpyF10VI GCNNNNNNNGC 2 cut(s) 347, 389
Hsp92II CATG 2 cut(s) 214, 315
LmnI GCTCC 2 cut(s) 37, 346
Lsp1109I GCAGC 3 cut(s) 122, 271, 359
LweI GCATC 2 cut(s) 97, 122
MaeI CTAG 1 cut(s) 117
MaeIII GTNAC 1 cut(s) 434
MhlI GDGCHC 1 cut(s) 464
MluCI AATT 1 cut(s) 417
MnlI CCTC 7 cut(s) 16, 28, 134, 188, 220, 242, 451
MseI TTAA 3 cut(s) 321, 386, 413
MslI CAYNNNNRTG 4 cut(s) 93, 156, 350, 429
MspR9I CCNGG 2 cut(s) 270, 330
MvaI CCWGG 2 cut(s) 270, 330
MwoI GCNNNNNNNGC 2 cut(s) 347, 389
NlaIII CATG 2 cut(s) 214, 315
NlaIV GGNNCC 3 cut(s) 8, 189, 462
NmuCI GTSAC 1 cut(s) 434
PceI AGGCCT 1 cut(s) 81
PfeI GAWTC 1 cut(s) 449
PfoI TCCNGGA 1 cut(s) 328
PkrI GCNGC 3 cut(s) 112, 286, 374
PpuMI RGGWCCY 1 cut(s) 187
Psp5II RGGWCCY 1 cut(s) 187
Psp6I CCWGG 2 cut(s) 268, 328
PspFI CCCAGC 1 cut(s) 392
PspGI CCWGG 2 cut(s) 268, 328
PspN4I GGNNCC 3 cut(s) 8, 189, 462
PspOMI GGGCCC 1 cut(s) 460
PspPI GGNCC 4 cut(s) 7, 187, 460, 461
PspPPI RGGWCCY 1 cut(s) 187
RsaI GTAC 2 cut(s) 70, 124
RsaNI GTAC 2 cut(s) 69, 123
RseI CAYNNNNRTG 4 cut(s) 93, 156, 350, 429
SaqAI TTAA 3 cut(s) 321, 386, 413
SatI GCNGC 3 cut(s) 111, 285, 373
Sau96I GGNCC 4 cut(s) 7, 187, 460, 461
ScaI AGTACT 1 cut(s) 124
ScrFI CCNGG 2 cut(s) 270, 330
SduI GDGCHC 1 cut(s) 464
SetI ASST 9 cut(s) 42, 189, 231, 286, 295, 343, 374, 394, 484
SfaNI GCATC 2 cut(s) 97, 122
SinI GGWCC 2 cut(s) 7, 187
SmiMI CAYNNNNRTG 4 cut(s) 93, 156, 350, 429
Sse9I AATT 1 cut(s) 417
SseBI AGGCCT 1 cut(s) 81
SsiI CCGC 2 cut(s) 254, 317
SspMI CTAG 1 cut(s) 117
StuI AGGCCT 1 cut(s) 81
StyD4I CCNGG 2 cut(s) 268, 328
TaqI TCGA 1 cut(s) 235
TasI AATT 1 cut(s) 417
TatI WGTACW 1 cut(s) 122
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 3 cut(s) 321, 386, 413
Tru9I TTAA 3 cut(s) 321, 386, 413
TseFI GTSAC 1 cut(s) 434
TseI GCWGC 3 cut(s) 110, 284, 372
Tsp45I GTSAC 1 cut(s) 434
TspDTI ATGAA 1 cut(s) 199
VpaK11BI GGWCC 2 cut(s) 7, 187
XagI CCTNNNNNAGG 1 cut(s) 182
XmiI GTMKAC 1 cut(s) 295
XspI CTAG 1 cut(s) 117
ZrmI AGTACT 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.