Rh6AG004800

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000686
Physical Location & Seq
Reverse (-)
710154 .. 711850
1697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG004800.1

Sequence Viewer

Length: 732 bp
ATGTTGACCAGCGGCACCCTCTCCACCGAAAAACAGCTGTATCGCCAACATTCCGACCACCTTCAGCTCCCAAAACTCGTGCCCGCTCGCTCCGATCGCCACGTGGTGCGCTATGTTCCCGCCGGCGCTGTCCCCGGCCAGAAAACACTGCCCGAGATTCGCGCCCTCGAACAAGCTACTTTGACTGTCTTCGATGAACTTGAGGACTTCGAAACCGTCGTCGTACTGGCACGGATATCGATGCTCCGGCGCGAGGCGGTAGTTGACGGAGACGACGACGGCGGGGAGCTCGCGAGCGAGGCGTTGACAGAAGTCGTCGTAGGGTTCAAGAGAGAGAGAGGGAGGACTAAGGAGAGATGTTCAAGAAGGGAGTGGAAGCTCGGTCGCACCAGAGGCTGTCAGGGGCTGACAGGAAGAAGCTCAAGAGGACCGTCAATTTCCCAGAGCTTCCGACGCCGATGTCGATGCTTTACTCCCTCCCAAGTTTATGCCCTCTGGAAGGTCCCTGATATCTTACCTTCTTTCATGCTGAAAGGGGGAGAGGTTTCTCAATATGTTATTGGAGGGGCGGATTTGATGTTTCCTGGTATCAGCATACCTGCTGAAGGTCTACCACCATTTATAGCTGCAGAAGCATGGGCTGTTAAAGTTCCAGGAAATCCAGCTCCCATTGCTGTGGGAACTACTGGTATGATCTTCCCTGAAAAGCTAGGTTGCGTGGAAAAGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SUI1

Protein Analysis

243

Amino Acids

26.68

Weight (kDa)

9.02

Isoelectric Point (pI)

48.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUA_elF2D PF26292 180 - 231 2.1e-12 elF2D, PUA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000686)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71350
fragaria_vesca FvH4_2g00430 FvH4_2g00430 FvH4_2g00430 FvH4_2g00430
malus_domestica MD10G1002600.v1.1
prunus_persica Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0249251 RchiOBHm_Chr6g0249261 RchiOBHm_Chr6g0249701 RchiOBHm_Chr6g0249761 RchiOBHm_Chr6g0249851 RchiOBHm_Chr6g0249861 RchiOBHm_Chr6g0249871 RchiOBHm_Chr6g0249891 RchiOBHm_Chr6g0249901 RchiOBHm_Chr6g0249911 RchiOBHm_Chr6g0249981 RchiOBHm_Chr6g0249991 RchiOBHm_Chr6g0250001 RchiOBHm_Chr6g0250151 RchiOBHm_Chr6g0250171 RchiOBHm_Chr6g0250191 RchiOBHm_Chr6g0250211 RchiOBHm_Chr6g0250221 RchiOBHm_Chr6g0250321 RchiOBHm_Chr6g0250391
rosa_laevigata RLG00000015543 RLG00000015548
rosa_multiflora Rmu_sc0000411.1_g000004 Rmu_sc0000411.1_g000009 Rmu_sc0000704.1_g000007 Rmu_sc0000704.1_g000011 Rmu_sc0001209.1_g000038 Rmu_sc0001209.1_g000042 Rmu_sc0001209.1_g000048 Rmu_sc0003293.1_g000005 Rmu_sc0004691.1_g000005 Rmu_sc0004691.1_g000007 Rmu_sc0004691.1_g000009 Rmu_sc0006032.1_g000001 Rmu_sc0006032.1_g000004 Rmu_sc0006032.1_g000005 Rmu_sc0008209.1_g000003 Rmu_sc0008209.1_g000010 Rmu_sc0009002.1_g000001 Rmu_sc0009002.1_g000005 Rmu_sc0009002.1_g000009
rosa_roxburghii Rroxscaffold_3G00218110 Rroxscaffold_7G00217270 Rroxscaffold_7G00217290 Rroxscaffold_7G00217300 Rroxscaffold_7G00217340
rosa_rugosa Rorug05G0494700 Rorug05G0495000 Rorug05G0496000 Rorug05G0498100 Rorug05G0498200
rosa_samantha Rh6AG000200 Rh6AG000300 Rh6AG004700 Rh6AG004800 Rh6AG005200 Rh6AG005300 Rh6AG005600 Rh6BG005500 Rh6BG005600 Rh6CG003700 Rh6DG003600
rosa_wichuraiana Rw6G000560 Rw6G000600 Rw6G000620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 459
Acc36I ACCTGC 1 cut(s) 607
AccB1I GGYRCC 1 cut(s) 14
AccBSI CCGCTC 1 cut(s) 86
AccI GTMKAC 1 cut(s) 610
AccII CGCG 3 cut(s) 162, 252, 293
AciI CCGC 6 cut(s) 12, 84, 120, 257, 282, 569
AcoI YGGCCR 1 cut(s) 136
AcuI CTGAAG 2 cut(s) 47, 624
AcvI CACGTG 1 cut(s) 103
AcyI GRCGYC 1 cut(s) 454
AdeI CACNNNGTG 1 cut(s) 106
AfaI GTAC 1 cut(s) 225
AfiI CCNNNNNNNGG 3 cut(s) 253, 499, 605
AgsI TTSAA 2 cut(s) 328, 363
AjnI CCWGG 2 cut(s) 583, 652
Alw21I GWGCWC 1 cut(s) 291
Alw26I GTCTC 1 cut(s) 264
AlwNI CAGNNNCTG 2 cut(s) 396, 406
Ama87I CYCGRG 1 cut(s) 152
AoxI GGCC 1 cut(s) 136
ApeKI GCWGC 1 cut(s) 626
ArsI GACNNNNNNTTYG 2 cut(s) 204, 236
AspLEI GCGC 4 cut(s) 111, 128, 164, 252
AspS9I GGNCC 2 cut(s) 428, 502
AsuC2I CCSGG 1 cut(s) 135
AsuII TTCGAA 1 cut(s) 210
AvaI CYCGRG 1 cut(s) 152
AvaII GGWCC 2 cut(s) 428, 502
BaeGI GKGCMC 1 cut(s) 84
BaeI ACNNNNGTAYC 2 cut(s) 23, 56
BanI GGYRCC 1 cut(s) 14
BanII GRGCYC 1 cut(s) 291
BauI CACGAG 1 cut(s) 77
BbrPI CACGTG 1 cut(s) 103
BbsI GAAGAC 1 cut(s) 181
Bbv12I GWGCWC 1 cut(s) 291
BbvI GCAGC 1 cut(s) 613
BceAI ACGGC 1 cut(s) 295
BcgI CGANNNNNNTGC 4 cut(s) 219, 253, 447, 481
BciT130I CCWGG 2 cut(s) 585, 654
BcnI CCSGG 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 264
BfaI CTAG 1 cut(s) 710
BfmI CTRYAG 1 cut(s) 627
BfoI RGCGCY 1 cut(s) 129
BfuAI ACCTGC 1 cut(s) 607
BisI GCNGC 2 cut(s) 13, 627
BlsI GCNGC 2 cut(s) 14, 628
Bme1390I CCNGG 3 cut(s) 135, 585, 654
Bme18I GGWCC 2 cut(s) 428, 502
BmeT110I CYCGRG 1 cut(s) 152
BmgT120I GGNCC 2 cut(s) 428, 502
BmiI GGNNCC 2 cut(s) 16, 504
BmrFI CCNGG 3 cut(s) 135, 585, 654
BmsI GCATC 2 cut(s) 231, 455
BoxI GACNNNNGTC 1 cut(s) 311
BpiI GAAGAC 1 cut(s) 181
Bpu14I TTCGAA 1 cut(s) 210
BpuEI CTTGAG 2 cut(s) 221, 406
BpuMI CCSGG 1 cut(s) 135
Bsa29I ATCGAT 1 cut(s) 239
BsaAI YACGTR 1 cut(s) 103
BsaHI GRCGYC 1 cut(s) 454
BsaJI CCNNGG 1 cut(s) 133
Bsc4I CCNNNNNNNGG 3 cut(s) 253, 499, 605
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 2 cut(s) 231, 691
Bse3DI GCAATG 1 cut(s) 669
BseBI CCWGG 2 cut(s) 585, 654
BseCI ATCGAT 1 cut(s) 239
BseDI CCNNGG 1 cut(s) 133
BseLI CCNNNNNNNGG 3 cut(s) 253, 499, 605
BseMI GCAATG 1 cut(s) 669
BseNI ACTGG 2 cut(s) 231, 691
BseSI GKGCMC 1 cut(s) 84
BseXI GCAGC 1 cut(s) 613
Bsh1236I CGCG 3 cut(s) 162, 252, 293
Bsh1285I CGRYCG 2 cut(s) 97, 385
BshFI GGCC 1 cut(s) 138
BshNI GGYRCC 1 cut(s) 14
BshVI ATCGAT 1 cut(s) 239
BsiEI CGRYCG 2 cut(s) 97, 385
BsiHKAI GWGCWC 1 cut(s) 291
BsiHKCI CYCGRG 1 cut(s) 152
BsiSI CCGG 3 cut(s) 123, 135, 247
BslFI GGGAC 2 cut(s) 116, 488
BslI CCNNNNNNNGG 3 cut(s) 253, 499, 605
BsmAI GTCTC 1 cut(s) 264
BsmBI CGTCTC 1 cut(s) 264
BsmFI GGGAC 2 cut(s) 116, 488
BsnI GGCC 1 cut(s) 138
BsoBI CYCGRG 1 cut(s) 152
Bsp119I TTCGAA 1 cut(s) 210
Bsp1286I GDGCHC 2 cut(s) 84, 291
Bsp143I GATC 2 cut(s) 94, 693
Bsp68I TCGCGA 1 cut(s) 293
BspACI CCGC 6 cut(s) 12, 84, 120, 257, 282, 569
BspANI GGCC 1 cut(s) 138
BspDI ATCGAT 1 cut(s) 239
BspFNI CGCG 3 cut(s) 162, 252, 293
BspLI GGNNCC 2 cut(s) 16, 504
BspMAI CTGCAG 1 cut(s) 631
BspMI ACCTGC 1 cut(s) 607
BspT104I TTCGAA 1 cut(s) 210
BspT107I GGYRCC 1 cut(s) 14
BsrBI CCGCTC 1 cut(s) 86
BsrDI GCAATG 1 cut(s) 669
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 2 cut(s) 231, 691
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 133
BssMI GATC 2 cut(s) 94, 693
BssNI GRCGYC 1 cut(s) 454
BssSI CACGAG 1 cut(s) 77
Bst2BI CACGAG 1 cut(s) 77
Bst2UI CCWGG 2 cut(s) 585, 654
Bst4CI ACNGT 3 cut(s) 187, 217, 432
BstACI GRCGYC 1 cut(s) 454
BstBAI YACGTR 1 cut(s) 103
BstBI TTCGAA 1 cut(s) 210
BstC8I GCNNGC 5 cut(s) 84, 88, 124, 291, 295
BstDEI CTNAG 1 cut(s) 348
BstENI CCTNNNNNAGG 2 cut(s) 497, 603
BstFNI CGCG 3 cut(s) 162, 252, 293
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 4 cut(s) 111, 128, 164, 252
BstKTI GATC 2 cut(s) 97, 696
BstMAI GTCTC 1 cut(s) 264
BstMBI GATC 2 cut(s) 94, 693
BstMCI CGRYCG 2 cut(s) 97, 385
BstMWI GCNNNNNNNGC 6 cut(s) 96, 299, 393, 453, 632, 671
BstNI CCWGG 2 cut(s) 585, 654
BstPAI GACNNNNGTC 1 cut(s) 311
BstSCI CCNGG 3 cut(s) 133, 583, 652
BstSFI CTRYAG 1 cut(s) 627
BstSLI GKGCMC 1 cut(s) 84
BstUI CGCG 3 cut(s) 162, 252, 293
BstV1I GCAGC 1 cut(s) 613
BstV2I GAAGAC 1 cut(s) 181
BstXI CCANNNNNNTGG 1 cut(s) 676
Bsu15I ATCGAT 1 cut(s) 239
BsuRI GGCC 1 cut(s) 138
BsuTUI ATCGAT 1 cut(s) 239
BtsI GCAGTG 1 cut(s) 146
BtsIMutI CAGTG 1 cut(s) 146
BtuMI TCGCGA 1 cut(s) 293
BveI ACCTGC 1 cut(s) 607
Cac8I GCNNGC 5 cut(s) 84, 88, 124, 291, 295
CaiI CAGNNNCTG 2 cut(s) 396, 406
CfoI GCGC 4 cut(s) 111, 128, 164, 252
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 2 cut(s) 428, 502
ClaI ATCGAT 1 cut(s) 239
CseI GACGC 1 cut(s) 462
Csp6I GTAC 1 cut(s) 224
CviAII CATG 2 cut(s) 526, 636
CviQI GTAC 1 cut(s) 224
DdeI CTNAG 1 cut(s) 348
DpnI GATC 2 cut(s) 96, 695
DpnII GATC 2 cut(s) 94, 693
DraIII CACNNNGTG 1 cut(s) 106
DrdI GACNNNNNNGTC 1 cut(s) 459
DseDI GACNNNNNNGTC 1 cut(s) 459
EaeI YGGCCR 1 cut(s) 136
EciI GGCGGA 1 cut(s) 584
Ecl136II GAGCTC 1 cut(s) 289
Eco24I GRGCYC 1 cut(s) 291
Eco32I GATATC 2 cut(s) 237, 511
Eco47I GGWCC 2 cut(s) 428, 502
Eco53kI GAGCTC 1 cut(s) 289
Eco57I CTGAAG 2 cut(s) 47, 624
Eco72I CACGTG 1 cut(s) 103
Eco88I CYCGRG 1 cut(s) 152
EcoICRI GAGCTC 1 cut(s) 289
EcoNI CCTNNNNNAGG 2 cut(s) 497, 603
EcoO109I RGGNCCY 1 cut(s) 502
EcoRII CCWGG 2 cut(s) 583, 652
EcoRV GATATC 2 cut(s) 237, 511
EcoT38I GRGCYC 1 cut(s) 291
Esp3I CGTCTC 1 cut(s) 264
FaeI CATG 2 cut(s) 529, 639
FaiI YATR 8 cut(s) 114, 489, 527, 555, 596, 623, 637, 692
FaqI GGGAC 2 cut(s) 116, 488
FatI CATG 2 cut(s) 525, 635
FauI CCCGC 3 cut(s) 91, 127, 275
FblI GTMKAC 1 cut(s) 610
Fnu4HI GCNGC 2 cut(s) 13, 627
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 2 cut(s) 13, 627
FspBI CTAG 1 cut(s) 710
GlaI GCGC 4 cut(s) 110, 127, 163, 251
GluI GCNGC 2 cut(s) 13, 627
HaeII RGCGCY 1 cut(s) 129
HaeIII GGCC 1 cut(s) 138
HapII CCGG 3 cut(s) 123, 135, 247
HgaI GACGC 1 cut(s) 462
HhaI GCGC 4 cut(s) 111, 128, 164, 252
Hin1I GRCGYC 1 cut(s) 454
Hin1II CATG 2 cut(s) 529, 639
Hin6I GCGC 4 cut(s) 109, 126, 162, 250
HinP1I GCGC 4 cut(s) 109, 126, 162, 250
HincII GTYRAC 3 cut(s) 6, 265, 306
HindII GTYRAC 3 cut(s) 6, 265, 306
HinfI GANTC 1 cut(s) 157
HpaII CCGG 3 cut(s) 123, 135, 247
Hpy166II GTNNAC 4 cut(s) 6, 265, 306, 611
Hpy188I TCNGA 3 cut(s) 55, 94, 452
Hpy188III TCNNGA 5 cut(s) 292, 328, 363, 423, 496
Hpy8I GTNNAC 4 cut(s) 6, 265, 306, 611
Hpy99I CGWCG 6 cut(s) 221, 224, 278, 281, 320, 456
HpyAV CCTTC 5 cut(s) 71, 360, 493, 528, 599
HpyCH4III ACNGT 3 cut(s) 187, 217, 432
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 1 cut(s) 629
HpyF10VI GCNNNNNNNGC 6 cut(s) 96, 299, 393, 453, 632, 671
HpyF3I CTNAG 1 cut(s) 348
HpySE526I ACGT 1 cut(s) 102
Hsp92I GRCGYC 1 cut(s) 454
Hsp92II CATG 2 cut(s) 529, 639
HspAI GCGC 4 cut(s) 109, 126, 162, 250
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
Kzo9I GATC 2 cut(s) 94, 693
LmnI GCTCC 5 cut(s) 72, 95, 249, 286, 670
Lsp1109I GCAGC 1 cut(s) 613
LweI GCATC 2 cut(s) 231, 455
MaeI CTAG 1 cut(s) 710
MaeII ACGT 1 cut(s) 102
MalI GATC 2 cut(s) 96, 695
MbiI CCGCTC 1 cut(s) 86
MboI GATC 2 cut(s) 94, 693
MboII GAAGA 3 cut(s) 181, 426, 688
MhlI GDGCHC 2 cut(s) 84, 291
MluCI AATT 1 cut(s) 435
MmeI TCCRAC 2 cut(s) 78, 475
MreI CGCCGGCG 1 cut(s) 122
MroNI GCCGGC 1 cut(s) 122
MseI TTAA 2 cut(s) 645, 730
MslI CAYNNNNRTG 1 cut(s) 674
MspA1I CMGCKG 2 cut(s) 12, 37
MspI CCGG 3 cut(s) 123, 135, 247
MspR9I CCNGG 3 cut(s) 135, 585, 654
MvaI CCWGG 2 cut(s) 585, 654
MvnI CGCG 3 cut(s) 162, 252, 293
MwoI GCNNNNNNNGC 6 cut(s) 96, 299, 393, 453, 632, 671
NaeI GCCGGC 1 cut(s) 124
NciI CCSGG 1 cut(s) 135
NdeII GATC 2 cut(s) 94, 693
NgoMIV GCCGGC 1 cut(s) 122
NlaIII CATG 2 cut(s) 529, 639
NlaIV GGNNCC 2 cut(s) 16, 504
NruI TCGCGA 1 cut(s) 293
NspV TTCGAA 1 cut(s) 210
PcsI WCGNNNNNNNCGW 3 cut(s) 216, 273, 460
PdiI GCCGGC 1 cut(s) 124
PfeI GAWTC 1 cut(s) 157
PfoI TCCNGGA 1 cut(s) 652
PkrI GCNGC 2 cut(s) 14, 628
Ple19I CGATCG 1 cut(s) 97
PmaCI CACGTG 1 cut(s) 103
PmlI CACGTG 1 cut(s) 103
Ppu21I YACGTR 1 cut(s) 103
PpuMI RGGWCCY 1 cut(s) 502
PshAI GACNNNNGTC 1 cut(s) 311
Psp124BI GAGCTC 1 cut(s) 291
Psp5II RGGWCCY 1 cut(s) 502
Psp6I CCWGG 2 cut(s) 583, 652
PspCI CACGTG 1 cut(s) 103
PspGI CCWGG 2 cut(s) 583, 652
PspN4I GGNNCC 2 cut(s) 16, 504
PspPI GGNCC 2 cut(s) 428, 502
PspPPI RGGWCCY 1 cut(s) 502
PstI CTGCAG 1 cut(s) 631
PstNI CAGNNNCTG 2 cut(s) 396, 406
PvuI CGATCG 1 cut(s) 97
PvuII CAGCTG 1 cut(s) 37
RruI TCGCGA 1 cut(s) 293
RsaI GTAC 1 cut(s) 225
RsaNI GTAC 1 cut(s) 224
RseI CAYNNNNRTG 1 cut(s) 674
SacI GAGCTC 1 cut(s) 291
SaqAI TTAA 2 cut(s) 645, 730
SatI GCNGC 2 cut(s) 13, 627
Sau3AI GATC 2 cut(s) 94, 693
Sau96I GGNCC 2 cut(s) 428, 502
ScrFI CCNGG 3 cut(s) 135, 585, 654
SduI GDGCHC 2 cut(s) 84, 291
SfaNI GCATC 2 cut(s) 231, 455
SfcI CTRYAG 1 cut(s) 627
SfuI TTCGAA 1 cut(s) 210
SgrAI CRCCGGYG 1 cut(s) 122
SinI GGWCC 2 cut(s) 428, 502
SmiMI CAYNNNNRTG 1 cut(s) 674
SmlI CTYRAG 2 cut(s) 200, 421
SmoI CTYRAG 2 cut(s) 200, 421
Sse9I AATT 1 cut(s) 435
SsiI CCGC 6 cut(s) 12, 84, 120, 257, 282, 569
SspMI CTAG 1 cut(s) 710
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 3 cut(s) 133, 583, 652
TaaI ACNGT 3 cut(s) 187, 217, 432
TaiI ACGT 1 cut(s) 105
TaqI TCGA 5 cut(s) 168, 192, 210, 239, 463
TaqII GACCGA 1 cut(s) 371
TasI AATT 1 cut(s) 435
TauI GCSGC 1 cut(s) 15
TfiI GAWTC 1 cut(s) 157
Tru1I TTAA 2 cut(s) 645, 730
Tru9I TTAA 2 cut(s) 645, 730
TscAI CASTG 1 cut(s) 153
TseI GCWGC 1 cut(s) 626
TspDTI ATGAA 2 cut(s) 210, 514
TspGWI ACGGA 2 cut(s) 247, 282
TspRI CASTG 1 cut(s) 153
VpaK11BI GGWCC 2 cut(s) 428, 502
XagI CCTNNNNNAGG 2 cut(s) 497, 603
XmiI GTMKAC 1 cut(s) 610
XspI CTAG 1 cut(s) 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.