RchiOBHm_Chr6g0250191

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000686
Physical Location & Seq
Reverse (-)
5842747 .. 5845182
2436 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22428

Sequence Viewer

Length: 1137 bp
ATGTTCCTTCAATCCCTCGTAGTACCTCTTCTGCCAATCCTGCAATGGATCAAATCCCCCAACAACCACAATCGTCGCCGGAAAATCCACCCCCGAAATATCCATCGCCTTCGGCCCGAACACATTCACCACCGGGTGGTCCCGATCCGACCCCTCCGGCAAAAAACGCCTTCCGCATCCATTCCGTCCGCTCTATGTTCACCAGCGGTACCTCTCTTACCACTTTTTCCTTCTGAATCATTATGCTTAATTGGTTATGGCCTTGCTTTTTTTTTGCATTTCCCCAGCTCCGAATGGTTTCACTCTGATTCTGGGTTTCATTCCAGGAAACCGTATATGTGTGATATTCATCTCTTGCAAAATTTCATGATCTTTAACTTGCGATTAGTTTGCTGCCAATTTAAGGTAGACCGGAGTTTACACTGTTACTTGGGAATATATTTTTTTTTCTTCAGGGAATCAGTTGAGGGCCCTTATGTGCCAAATGCAGGTTTCTTAGAAGATGCTGTTTTTGAGGATCCTACCTTGTCATCAGCTCAAATTTCTGATTCATATAATGGTGCAAATGATGGATCACATGATCAACTGAATGGCATTGATGATAAGGAAATGGGGCAACATGTTGATGTTGCTGATGTGCAGTCTGAACCAAGTTCTGCTTCAGTTGCACCGATTGATGTTAGGAATGAGATTGCTGAGGAAGTAACTGCAAATGTAGGTGATCTGAAGCTAACAGATGATGATTCTGCTAATCAGTCGACTGGGGAGGATCAACATCCTCTTTCTGCCGAGGGTGTGGACATGCTTTTGGAAAAATGCCTTTTGCAAGCATTGCATACAACTGTCAAAGACAAAGACCTTCCCATGCCCGGAAGCACTCTATGGTCAAACTATGTTTTACCTTGTAGGCCTTCAGGCATCACACTAGACATCAAGAAATCATCACACAAGAAATTATCAAAGTGGTTACAAGCTAAATGTTCTACAGGATTGATTAGAGTGAAGGAAGATAAATATAAGAAGGAGTCTGTATTACTTTCTATTGACCGCAGCCATCCAGATTACTCATCTTTCAAGCCAGAGAAACAACAAGTTGAAGAAGCTGCATGTTCAAACTGGTGTTCCTGCTGTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SUI1

Protein Analysis

378

Amino Acids

42.61

Weight (kDa)

6.27

Isoelectric Point (pI)

53.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_eIF2D PF25304 267 - 345 5.8e-29 eIF2D winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000686)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71350
fragaria_vesca FvH4_2g00430 FvH4_2g00430 FvH4_2g00430 FvH4_2g00430
malus_domestica MD10G1002600.v1.1
prunus_persica Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0249251 RchiOBHm_Chr6g0249261 RchiOBHm_Chr6g0249701 RchiOBHm_Chr6g0249761 RchiOBHm_Chr6g0249851 RchiOBHm_Chr6g0249861 RchiOBHm_Chr6g0249871 RchiOBHm_Chr6g0249891 RchiOBHm_Chr6g0249901 RchiOBHm_Chr6g0249911 RchiOBHm_Chr6g0249981 RchiOBHm_Chr6g0249991 RchiOBHm_Chr6g0250001 RchiOBHm_Chr6g0250151 RchiOBHm_Chr6g0250171 RchiOBHm_Chr6g0250191 RchiOBHm_Chr6g0250211 RchiOBHm_Chr6g0250221 RchiOBHm_Chr6g0250321 RchiOBHm_Chr6g0250391
rosa_laevigata RLG00000015543 RLG00000015548
rosa_multiflora Rmu_sc0000411.1_g000004 Rmu_sc0000411.1_g000009 Rmu_sc0000704.1_g000007 Rmu_sc0000704.1_g000011 Rmu_sc0001209.1_g000038 Rmu_sc0001209.1_g000042 Rmu_sc0001209.1_g000048 Rmu_sc0003293.1_g000005 Rmu_sc0004691.1_g000005 Rmu_sc0004691.1_g000007 Rmu_sc0004691.1_g000009 Rmu_sc0006032.1_g000001 Rmu_sc0006032.1_g000004 Rmu_sc0006032.1_g000005 Rmu_sc0008209.1_g000003 Rmu_sc0008209.1_g000010 Rmu_sc0009002.1_g000001 Rmu_sc0009002.1_g000005 Rmu_sc0009002.1_g000009
rosa_roxburghii Rroxscaffold_3G00218110 Rroxscaffold_7G00217270 Rroxscaffold_7G00217290 Rroxscaffold_7G00217300 Rroxscaffold_7G00217340
rosa_rugosa Rorug05G0494700 Rorug05G0495000 Rorug05G0496000 Rorug05G0498100 Rorug05G0498200
rosa_samantha Rh6AG000200 Rh6AG000300 Rh6AG004700 Rh6AG004800 Rh6AG005200 Rh6AG005300 Rh6AG005600 Rh6BG005500 Rh6BG005600 Rh6CG003700 Rh6DG003600
rosa_wichuraiana Rw6G000560 Rw6G000600 Rw6G000620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 479
Acc65I GGTACC 1 cut(s) 208
AccB1I GGYRCC 1 cut(s) 208
AccB7I CCANNNNNTGG 1 cut(s) 136
AccBSI CCGCTC 1 cut(s) 191
AccI GTMKAC 2 cut(s) 408, 758
AciI CCGC 4 cut(s) 174, 189, 206, 1048
AclWI GGATC 6 cut(s) 56, 139, 512, 525, 580, 777
AcsI RAATTY 2 cut(s) 361, 540
AcuI CTGAAG 4 cut(s) 436, 645, 746, 897
AdeI CACNNNGTG 1 cut(s) 136
AfaI GTAC 2 cut(s) 24, 210
AfiI CCNNNNNNNGG 4 cut(s) 136, 403, 488, 869
AflIII ACRYGT 1 cut(s) 619
AgsI TTSAA 4 cut(s) 11, 1075, 1097, 1113
AjnI CCWGG 1 cut(s) 323
AjuI GAANNNNNNNTTGG 2 cut(s) 643, 675
AluBI AGCT 5 cut(s) 288, 536, 730, 974, 1103
AluI AGCT 5 cut(s) 288, 536, 730, 974, 1103
AlwI GGATC 6 cut(s) 56, 139, 512, 525, 580, 777
AoxI GGCC 4 cut(s) 113, 259, 469, 908
ApaI GGGCCC 1 cut(s) 473
ApeKI GCWGC 3 cut(s) 393, 1050, 1103
ApoI RAATTY 2 cut(s) 361, 540
Asp700I GAANNNNTTC 2 cut(s) 123, 297
Asp718I GGTACC 1 cut(s) 208
AspS9I GGNCC 4 cut(s) 114, 139, 469, 470
AsuC2I CCSGG 2 cut(s) 134, 870
AsuHPI GGTGA 3 cut(s) 119, 192, 731
AvaII GGWCC 1 cut(s) 139
BaeGI GKGCMC 1 cut(s) 473
BamHI GGATCC 1 cut(s) 517
BanI GGYRCC 1 cut(s) 208
BanII GRGCYC 1 cut(s) 473
BarI GAAGNNNNNNTAC 2 cut(s) 12, 44
BbvCI CCTCAGC 1 cut(s) 696
BbvI GCAGC 3 cut(s) 380, 1062, 1090
BccI CCATC 3 cut(s) 111, 563, 1062
BcgI CGANNNNNNTGC 2 cut(s) 372, 406
BciT130I CCWGG 1 cut(s) 325
BclI TGATCA 1 cut(s) 580
BcnI CCSGG 2 cut(s) 134, 870
BfaI CTAG 1 cut(s) 926
BfmI CTRYAG 1 cut(s) 984
BfuAI ACCTGC 1 cut(s) 479
BisI GCNGC 3 cut(s) 394, 1051, 1104
BlsI GCNGC 3 cut(s) 395, 1052, 1105
Bme1390I CCNGG 3 cut(s) 134, 325, 870
Bme18I GGWCC 1 cut(s) 139
BmgT120I GGNCC 4 cut(s) 114, 139, 469, 470
BmiI GGNNCC 4 cut(s) 141, 210, 471, 519
BmrFI CCNGG 3 cut(s) 134, 325, 870
BmrI ACTGGG 1 cut(s) 771
BmsI GCATC 3 cut(s) 185, 493, 927
BmuI ACTGGG 1 cut(s) 771
Bpu10I CCTNAGC 1 cut(s) 696
BpuMI CCSGG 2 cut(s) 134, 870
BsaBI GATNNNNATC 2 cut(s) 348, 774
BsaJI CCNNGG 1 cut(s) 789
BsaWI WCCGGW 1 cut(s) 411
Bsc4I CCNNNNNNNGG 4 cut(s) 136, 403, 488, 869
Bse1I ACTGG 2 cut(s) 766, 1121
Bse3DI GCAATG 2 cut(s) 50, 830
Bse8I GATNNNNATC 2 cut(s) 348, 774
BseBI CCWGG 1 cut(s) 325
BseDI CCNNGG 1 cut(s) 789
BseGI GGATG 3 cut(s) 176, 775, 1054
BseJI GATNNNNATC 2 cut(s) 348, 774
BseLI CCNNNNNNNGG 4 cut(s) 136, 403, 488, 869
BseMI GCAATG 2 cut(s) 50, 830
BseMII CTCAG 1 cut(s) 687
BseNI ACTGG 2 cut(s) 766, 1121
BseSI GKGCMC 1 cut(s) 473
BseXI GCAGC 3 cut(s) 380, 1062, 1090
BseYI CCCAGC 1 cut(s) 284
BsgI GTGCAG 1 cut(s) 659
BshFI GGCC 4 cut(s) 115, 261, 471, 910
BshNI GGYRCC 1 cut(s) 208
BsiSI CCGG 5 cut(s) 79, 133, 157, 412, 870
BslFI GGGAC 1 cut(s) 125
BslI CCNNNNNNNGG 4 cut(s) 136, 403, 488, 869
BsmFI GGGAC 1 cut(s) 125
BsnI GGCC 4 cut(s) 115, 261, 471, 910
Bsp120I GGGCCC 1 cut(s) 469
Bsp1286I GDGCHC 1 cut(s) 473
Bsp143I GATC 8 cut(s) 48, 144, 369, 517, 572, 580, 721, 769
BspACI CCGC 4 cut(s) 174, 189, 206, 1048
BspANI GGCC 4 cut(s) 115, 261, 471, 910
BspCNI CTCAG 1 cut(s) 688
BspHI TCATGA 1 cut(s) 366
BspLI GGNNCC 4 cut(s) 141, 210, 471, 519
BspMI ACCTGC 1 cut(s) 479
BspPI GGATC 6 cut(s) 56, 139, 512, 525, 580, 777
BspT107I GGYRCC 1 cut(s) 208
BsrBI CCGCTC 1 cut(s) 191
BsrDI GCAATG 2 cut(s) 50, 830
BsrI ACTGG 2 cut(s) 766, 1121
BssECI CCNNGG 1 cut(s) 789
BssMI GATC 8 cut(s) 48, 144, 369, 517, 572, 580, 721, 769
Bst2UI CCWGG 1 cut(s) 325
Bst4CI ACNGT 3 cut(s) 333, 425, 844
Bst6I CTCTTC 1 cut(s) 33
BstAPI GCANNNNNTGC 1 cut(s) 832
BstC8I GCNNGC 1 cut(s) 828
BstDEI CTNAG 2 cut(s) 496, 696
BstF5I GGATG 3 cut(s) 176, 775, 1054
BstKTI GATC 8 cut(s) 51, 147, 372, 520, 575, 583, 724, 772
BstMBI GATC 8 cut(s) 48, 144, 369, 517, 572, 580, 721, 769
BstMWI GCNNNNNNNGC 4 cut(s) 40, 166, 665, 832
BstNI CCWGG 1 cut(s) 325
BstNSI RCATGY 3 cut(s) 623, 805, 1110
BstSCI CCNGG 3 cut(s) 132, 323, 868
BstSFI CTRYAG 1 cut(s) 984
BstSLI GKGCMC 1 cut(s) 473
BstV1I GCAGC 3 cut(s) 380, 1062, 1090
BstX2I RGATCY 1 cut(s) 517
BstYI RGATCY 1 cut(s) 517
BsuRI GGCC 4 cut(s) 115, 261, 471, 910
BtgZI GCGATG 1 cut(s) 89
BtsCI GGATG 3 cut(s) 176, 775, 1054
BtsIMutI CAGTG 1 cut(s) 421
BveI ACCTGC 1 cut(s) 479
Cac8I GCNNGC 1 cut(s) 828
CciI TCATGA 1 cut(s) 366
Cfr13I GGNCC 4 cut(s) 114, 139, 469, 470
Csp6I GTAC 2 cut(s) 23, 209
CviAII CATG 6 cut(s) 367, 578, 620, 802, 865, 1107
CviQI GTAC 2 cut(s) 23, 209
DdeI CTNAG 2 cut(s) 496, 696
DpnI GATC 8 cut(s) 50, 146, 371, 519, 574, 582, 723, 771
DpnII GATC 8 cut(s) 48, 144, 369, 517, 572, 580, 721, 769
DraIII CACNNNGTG 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
Eco147I AGGCCT 1 cut(s) 910
Eco24I GRGCYC 1 cut(s) 473
Eco47I GGWCC 1 cut(s) 139
Eco57I CTGAAG 4 cut(s) 436, 645, 746, 897
EcoO109I RGGNCCY 2 cut(s) 469, 470
EcoRII CCWGG 1 cut(s) 323
EcoT38I GRGCYC 1 cut(s) 473
FaeI CATG 6 cut(s) 370, 581, 623, 805, 868, 1110
FalI AAGNNNNNCTT 2 cut(s) 643, 675
FaqI GGGAC 1 cut(s) 125
FatI CATG 6 cut(s) 366, 577, 619, 801, 864, 1106
FbaI TGATCA 1 cut(s) 580
FblI GTMKAC 2 cut(s) 408, 758
Fnu4HI GCNGC 3 cut(s) 394, 1051, 1104
FokI GGATG 3 cut(s) 163, 762, 1041
FriOI GRGCYC 1 cut(s) 473
Fsp4HI GCNGC 3 cut(s) 394, 1051, 1104
FspBI CTAG 1 cut(s) 926
GluI GCNGC 3 cut(s) 394, 1051, 1104
GsaI CCCAGC 1 cut(s) 288
HaeIII GGCC 4 cut(s) 115, 261, 471, 910
HapII CCGG 5 cut(s) 79, 133, 157, 412, 870
Hin1II CATG 6 cut(s) 370, 581, 623, 805, 868, 1110
HincII GTYRAC 1 cut(s) 759
HindII GTYRAC 1 cut(s) 759
HinfI GANTC 6 cut(s) 236, 308, 458, 548, 743, 1025
HpaII CCGG 5 cut(s) 79, 133, 157, 412, 870
HphI GGTGA 3 cut(s) 119, 192, 731
Hpy166II GTNNAC 5 cut(s) 200, 409, 419, 759, 799
Hpy188I TCNGA 7 cut(s) 149, 235, 292, 307, 547, 646, 726
Hpy188III TCNNGA 4 cut(s) 142, 367, 934, 1058
Hpy8I GTNNAC 5 cut(s) 200, 409, 419, 759, 799
Hpy99I CGWCG 1 cut(s) 78
HpyAV CCTTC 8 cut(s) 17, 119, 180, 240, 869, 921, 997, 1015
HpyCH4III ACNGT 3 cut(s) 333, 425, 844
HpyF10VI GCNNNNNNNGC 4 cut(s) 40, 166, 665, 832
HpyF3I CTNAG 2 cut(s) 496, 696
Hsp92II CATG 6 cut(s) 370, 581, 623, 805, 868, 1110
KpnI GGTACC 1 cut(s) 212
Ksp22I TGATCA 1 cut(s) 580
Kzo9I GATC 8 cut(s) 48, 144, 369, 517, 572, 580, 721, 769
LmnI GCTCC 1 cut(s) 293
Lsp1109I GCAGC 3 cut(s) 380, 1062, 1090
LweI GCATC 3 cut(s) 185, 493, 927
MaeI CTAG 1 cut(s) 926
MaeIII GTNAC 3 cut(s) 425, 703, 966
MalI GATC 8 cut(s) 50, 146, 371, 519, 574, 582, 723, 771
MbiI CCGCTC 1 cut(s) 191
MboI GATC 8 cut(s) 48, 144, 369, 517, 572, 580, 721, 769
MboII GAAGA 5 cut(s) 20, 442, 512, 1019, 1109
MflI RGATCY 1 cut(s) 517
MhlI GDGCHC 1 cut(s) 473
MluCI AATT 5 cut(s) 249, 361, 398, 540, 953
MlyI GAGTC 1 cut(s) 1034
MmeI TCCRAC 1 cut(s) 172
MroXI GAANNNNTTC 2 cut(s) 123, 297
MseI TTAA 3 cut(s) 248, 375, 402
MslI CAYNNNNRTG 1 cut(s) 624
MspA1I CMGCKG 1 cut(s) 206
MspI CCGG 5 cut(s) 79, 133, 157, 412, 870
MspR9I CCNGG 3 cut(s) 134, 325, 870
MvaI CCWGG 1 cut(s) 325
MwoI GCNNNNNNNGC 4 cut(s) 40, 166, 665, 832
NciI CCSGG 2 cut(s) 134, 870
NdeII GATC 8 cut(s) 48, 144, 369, 517, 572, 580, 721, 769
NlaIII CATG 6 cut(s) 370, 581, 623, 805, 868, 1110
NlaIV GGNNCC 4 cut(s) 141, 210, 471, 519
NmeAIII GCCGAG 1 cut(s) 814
NspI RCATGY 3 cut(s) 623, 805, 1110
PagI TCATGA 1 cut(s) 366
PceI AGGCCT 1 cut(s) 910
PciI ACATGT 1 cut(s) 619
PdmI GAANNNNTTC 2 cut(s) 123, 297
PfeI GAWTC 5 cut(s) 236, 308, 458, 548, 743
PflMI CCANNNNNTGG 1 cut(s) 136
PfoI TCCNGGA 1 cut(s) 323
PkrI GCNGC 3 cut(s) 395, 1052, 1105
PleI GAGTC 1 cut(s) 1033
PpsI GAGTC 1 cut(s) 1033
PscI ACATGT 1 cut(s) 619
Psp6I CCWGG 1 cut(s) 323
PspFI CCCAGC 1 cut(s) 284
PspGI CCWGG 1 cut(s) 323
PspN4I GGNNCC 4 cut(s) 141, 210, 471, 519
PspOMI GGGCCC 1 cut(s) 469
PspPI GGNCC 4 cut(s) 114, 139, 469, 470
PsuI RGATCY 1 cut(s) 517
RsaI GTAC 2 cut(s) 24, 210
RsaNI GTAC 2 cut(s) 23, 209
RseI CAYNNNNRTG 1 cut(s) 624
SalI GTCGAC 1 cut(s) 757
SaqAI TTAA 3 cut(s) 248, 375, 402
SatI GCNGC 3 cut(s) 394, 1051, 1104
Sau3AI GATC 8 cut(s) 48, 144, 369, 517, 572, 580, 721, 769
Sau96I GGNCC 4 cut(s) 114, 139, 469, 470
SchI GAGTC 1 cut(s) 1034
ScrFI CCNGG 3 cut(s) 134, 325, 870
SduI GDGCHC 1 cut(s) 473
SfaNI GCATC 3 cut(s) 185, 493, 927
SfcI CTRYAG 1 cut(s) 984
SinI GGWCC 1 cut(s) 139
SmiMI CAYNNNNRTG 1 cut(s) 624
Sse9I AATT 5 cut(s) 249, 361, 398, 540, 953
SseBI AGGCCT 1 cut(s) 910
SsiI CCGC 4 cut(s) 174, 189, 206, 1048
SspMI CTAG 1 cut(s) 926
StuI AGGCCT 1 cut(s) 910
StyD4I CCNGG 3 cut(s) 132, 323, 868
TaaI ACNGT 3 cut(s) 333, 425, 844
TaqI TCGA 1 cut(s) 758
TasI AATT 5 cut(s) 249, 361, 398, 540, 953
TfiI GAWTC 5 cut(s) 236, 308, 458, 548, 743
Tru1I TTAA 3 cut(s) 248, 375, 402
Tru9I TTAA 3 cut(s) 248, 375, 402
TscAI CASTG 1 cut(s) 428
TseI GCWGC 3 cut(s) 393, 1050, 1103
TspDTI ATGAA 4 cut(s) 308, 338, 355, 540
TspGWI ACGGA 1 cut(s) 174
TspRI CASTG 1 cut(s) 428
Van91I CCANNNNNTGG 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 139
XapI RAATTY 2 cut(s) 361, 540
XceI RCATGY 3 cut(s) 623, 805, 1110
XcmI CCANNNNNNNNNTGG 1 cut(s) 42
XmiI GTMKAC 2 cut(s) 408, 758
XmnI GAANNNNTTC 2 cut(s) 123, 297
XspI CTAG 1 cut(s) 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.