eukaryotic translation initiation factor
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1008 |
| Acc36I | ACCTGC | 1 cut(s) | 1156 |
| AccB1I | GGYRCC | 1 cut(s) | 531 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 460 |
| AccBSI | CCGCTC | 2 cut(s) | 514, 565 |
| AccI | GTMKAC | 1 cut(s) | 1159 |
| AccII | CGCG | 6 cut(s) | 248, 266, 432, 711, 801, 842 |
| AclWI | GGATC | 1 cut(s) | 381 |
| AcoI | YGGCCR | 2 cut(s) | 88, 685 |
| AcuI | CTGAAG | 2 cut(s) | 596, 1173 |
| AcvI | CACGTG | 1 cut(s) | 652 |
| AcyI | GRCGYC | 1 cut(s) | 1003 |
| AdeI | CACNNNGTG | 2 cut(s) | 460, 655 |
| AfaI | GTAC | 1 cut(s) | 774 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 90, 109, 242, 460, 802, 1048, 1154 |
| AgsI | TTSAA | 3 cut(s) | 542, 877, 912 |
| AjnI | CCWGG | 3 cut(s) | 131, 1132, 1201 |
| Alw21I | GWGCWC | 2 cut(s) | 171, 840 |
| Alw26I | GTCTC | 2 cut(s) | 554, 813 |
| AlwI | GGATC | 1 cut(s) | 381 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 945, 955 |
| Ama87I | CYCGRG | 2 cut(s) | 63, 701 |
| AoxI | GGCC | 8 cut(s) | 67, 81, 88, 127, 153, 437, 685, 1282 |
| ApaI | GGGCCC | 1 cut(s) | 1286 |
| ApeKI | GCWGC | 2 cut(s) | 178, 1175 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 753, 785 |
| AspLEI | GCGC | 6 cut(s) | 248, 250, 660, 677, 713, 801 |
| AsuC2I | CCSGG | 2 cut(s) | 458, 684 |
| AsuII | TTCGAA | 1 cut(s) | 759 |
| AvaI | CYCGRG | 2 cut(s) | 63, 701 |
| AvaII | GGWCC | 6 cut(s) | 97, 136, 463, 473, 977, 1051 |
| BaeGI | GKGCMC | 2 cut(s) | 633, 1286 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 572, 605 |
| BanI | GGYRCC | 1 cut(s) | 531 |
| BanII | GRGCYC | 3 cut(s) | 171, 840, 1286 |
| BauI | CACGAG | 1 cut(s) | 626 |
| BbrPI | CACGTG | 1 cut(s) | 652 |
| BbsI | GAAGAC | 1 cut(s) | 730 |
| Bbv12I | GWGCWC | 2 cut(s) | 171, 840 |
| BbvI | GCAGC | 2 cut(s) | 165, 1162 |
| BccI | CCATC | 4 cut(s) | 147, 299, 310, 341 |
| BceAI | ACGGC | 2 cut(s) | 169, 844 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 768, 802, 996, 1030 |
| BciT130I | CCWGG | 3 cut(s) | 133, 1134, 1203 |
| BcnI | CCSGG | 2 cut(s) | 458, 684 |
| BcoDI | GTCTC | 2 cut(s) | 554, 813 |
| BfaI | CTAG | 2 cut(s) | 45, 1259 |
| BfmI | CTRYAG | 1 cut(s) | 1176 |
| BfoI | RGCGCY | 1 cut(s) | 678 |
| BfuAI | ACCTGC | 1 cut(s) | 1156 |
| BisI | GCNGC | 5 cut(s) | 156, 179, 182, 530, 1176 |
| BlsI | GCNGC | 5 cut(s) | 157, 180, 183, 531, 1177 |
| Bme1390I | CCNGG | 5 cut(s) | 133, 458, 684, 1134, 1203 |
| Bme18I | GGWCC | 6 cut(s) | 97, 136, 463, 473, 977, 1051 |
| BmeT110I | CYCGRG | 2 cut(s) | 63, 701 |
| BmrFI | CCNGG | 5 cut(s) | 133, 458, 684, 1134, 1203 |
| BmsI | GCATC | 2 cut(s) | 780, 1004 |
| BoxI | GACNNNNGTC | 1 cut(s) | 860 |
| BpiI | GAAGAC | 1 cut(s) | 730 |
| Bpu14I | TTCGAA | 1 cut(s) | 759 |
| BpuEI | CTTGAG | 2 cut(s) | 770, 955 |
| BpuMI | CCSGG | 2 cut(s) | 458, 684 |
| Bsa29I | ATCGAT | 1 cut(s) | 788 |
| BsaAI | YACGTR | 1 cut(s) | 652 |
| BsaHI | GRCGYC | 1 cut(s) | 1003 |
| BsaJI | CCNNGG | 5 cut(s) | 131, 132, 150, 185, 682 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 208, 238, 305, 335 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 90, 109, 242, 460, 802, 1048, 1154 |
| Bse118I | RCCGGY | 2 cut(s) | 86, 671 |
| Bse1I | ACTGG | 2 cut(s) | 780, 1240 |
| Bse3DI | GCAATG | 1 cut(s) | 1218 |
| BseBI | CCWGG | 3 cut(s) | 133, 1134, 1203 |
| BseCI | ATCGAT | 1 cut(s) | 788 |
| BseDI | CCNNGG | 5 cut(s) | 131, 132, 150, 185, 682 |
| BseGI | GGATG | 3 cut(s) | 310, 352, 499 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 90, 109, 242, 460, 802, 1048, 1154 |
| BseMI | GCAATG | 1 cut(s) | 1218 |
| BseNI | ACTGG | 2 cut(s) | 780, 1240 |
| BsePI | GCGCGC | 1 cut(s) | 246 |
| BseRI | GAGGAG | 2 cut(s) | 28, 557 |
| BseSI | GKGCMC | 2 cut(s) | 633, 1286 |
| BseX3I | CGGCCG | 1 cut(s) | 88 |
| BseXI | GCAGC | 2 cut(s) | 165, 1162 |
| BseYI | CCCAGC | 1 cut(s) | 1286 |
| BsgI | GTGCAG | 1 cut(s) | 100 |
| Bsh1236I | CGCG | 6 cut(s) | 248, 266, 432, 711, 801, 842 |
| Bsh1285I | CGRYCG | 4 cut(s) | 91, 471, 646, 934 |
| BshFI | GGCC | 8 cut(s) | 69, 83, 90, 129, 155, 439, 687, 1284 |
| BshNI | GGYRCC | 1 cut(s) | 531 |
| BshVI | ATCGAT | 1 cut(s) | 788 |
| BsiEI | CGRYCG | 4 cut(s) | 91, 471, 646, 934 |
| BsiHKAI | GWGCWC | 2 cut(s) | 171, 840 |
| BsiHKCI | CYCGRG | 2 cut(s) | 63, 701 |
| BsiSI | CCGG | 9 cut(s) | 87, 91, 243, 403, 457, 481, 672, 684, 796 |
| BslFI | GGGAC | 4 cut(s) | 149, 449, 665, 1037 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 90, 109, 242, 460, 802, 1048, 1154 |
| BsmAI | GTCTC | 2 cut(s) | 554, 813 |
| BsmBI | CGTCTC | 2 cut(s) | 554, 813 |
| BsmFI | GGGAC | 4 cut(s) | 149, 449, 665, 1037 |
| BsnI | GGCC | 8 cut(s) | 69, 83, 90, 129, 155, 439, 687, 1284 |
| BsoBI | CYCGRG | 2 cut(s) | 63, 701 |
| Bsp119I | TTCGAA | 1 cut(s) | 759 |
| Bsp120I | GGGCCC | 1 cut(s) | 1282 |
| Bsp1286I | GDGCHC | 4 cut(s) | 171, 633, 840, 1286 |
| Bsp143I | GATC | 4 cut(s) | 373, 468, 643, 1242 |
| Bsp68I | TCGCGA | 1 cut(s) | 842 |
| BspANI | GGCC | 8 cut(s) | 69, 83, 90, 129, 155, 439, 687, 1284 |
| BspDI | ATCGAT | 1 cut(s) | 788 |
| BspFNI | CGCG | 6 cut(s) | 248, 266, 432, 711, 801, 842 |
| BspMAI | CTGCAG | 1 cut(s) | 1180 |
| BspMI | ACCTGC | 1 cut(s) | 1156 |
| BspPI | GGATC | 1 cut(s) | 381 |
| BspT104I | TTCGAA | 1 cut(s) | 759 |
| BspT107I | GGYRCC | 1 cut(s) | 531 |
| BsrBI | CCGCTC | 2 cut(s) | 514, 565 |
| BsrDI | GCAATG | 1 cut(s) | 1218 |
| BsrFI | RCCGGY | 2 cut(s) | 86, 671 |
| BsrI | ACTGG | 2 cut(s) | 780, 1240 |
| BssAI | RCCGGY | 2 cut(s) | 86, 671 |
| BssECI | CCNNGG | 5 cut(s) | 131, 132, 150, 185, 682 |
| BssHII | GCGCGC | 1 cut(s) | 246 |
| BssMI | GATC | 4 cut(s) | 373, 468, 643, 1242 |
| BssNI | GRCGYC | 1 cut(s) | 1003 |
| BssSI | CACGAG | 1 cut(s) | 626 |
| BssT1I | CCWWGG | 1 cut(s) | 150 |
| Bst2BI | CACGAG | 1 cut(s) | 626 |
| Bst2UI | CCWGG | 3 cut(s) | 133, 1134, 1203 |
| Bst4CI | ACNGT | 4 cut(s) | 232, 736, 766, 981 |
| Bst6I | CTCTTC | 1 cut(s) | 543 |
| BstACI | GRCGYC | 1 cut(s) | 1003 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 114 |
| BstBAI | YACGTR | 1 cut(s) | 652 |
| BstBI | TTCGAA | 1 cut(s) | 759 |
| BstC8I | GCNNGC | 6 cut(s) | 248, 633, 637, 673, 840, 844 |
| BstDEI | CTNAG | 2 cut(s) | 77, 897 |
| BstDSI | CCRYGG | 1 cut(s) | 185 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1046, 1152 |
| BstF5I | GGATG | 3 cut(s) | 310, 352, 499 |
| BstFNI | CGCG | 6 cut(s) | 248, 266, 432, 711, 801, 842 |
| BstH2I | RGCGCY | 1 cut(s) | 678 |
| BstHHI | GCGC | 6 cut(s) | 248, 250, 660, 677, 713, 801 |
| BstKTI | GATC | 4 cut(s) | 376, 471, 646, 1245 |
| BstMAI | GTCTC | 2 cut(s) | 554, 813 |
| BstMBI | GATC | 4 cut(s) | 373, 468, 643, 1242 |
| BstMCI | CGRYCG | 4 cut(s) | 91, 471, 646, 934 |
| BstNI | CCWGG | 3 cut(s) | 133, 1134, 1203 |
| BstNSI | RCATGY | 1 cut(s) | 329 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 860 |
| BstSCI | CCNGG | 5 cut(s) | 131, 456, 682, 1132, 1201 |
| BstSFI | CTRYAG | 1 cut(s) | 1176 |
| BstSLI | GKGCMC | 2 cut(s) | 633, 1286 |
| BstUI | CGCG | 6 cut(s) | 248, 266, 432, 711, 801, 842 |
| BstV1I | GCAGC | 2 cut(s) | 165, 1162 |
| BstV2I | GAAGAC | 1 cut(s) | 730 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1225 |
| BstZI | CGGCCG | 1 cut(s) | 88 |
| Bsu15I | ATCGAT | 1 cut(s) | 788 |
| BsuRI | GGCC | 8 cut(s) | 69, 83, 90, 129, 155, 439, 687, 1284 |
| BsuTUI | ATCGAT | 1 cut(s) | 788 |
| BtgI | CCRYGG | 1 cut(s) | 185 |
| BtsCI | GGATG | 3 cut(s) | 310, 352, 499 |
| BtsI | GCAGTG | 1 cut(s) | 695 |
| BtsIMutI | CAGTG | 2 cut(s) | 237, 695 |
| BtuMI | TCGCGA | 1 cut(s) | 842 |
| BveI | ACCTGC | 1 cut(s) | 1156 |
| Cac8I | GCNNGC | 6 cut(s) | 248, 633, 637, 673, 840, 844 |
| CaiI | CAGNNNCTG | 2 cut(s) | 945, 955 |
| CfoI | GCGC | 6 cut(s) | 248, 250, 660, 677, 713, 801 |
| Cfr10I | RCCGGY | 2 cut(s) | 86, 671 |
| ClaI | ATCGAT | 1 cut(s) | 788 |
| CseI | GACGC | 1 cut(s) | 1011 |
| Csp6I | GTAC | 1 cut(s) | 773 |
| CviAII | CATG | 3 cut(s) | 326, 1075, 1185 |
| CviQI | GTAC | 1 cut(s) | 773 |
| DdeI | CTNAG | 2 cut(s) | 77, 897 |
| DpnI | GATC | 4 cut(s) | 375, 470, 645, 1244 |
| DpnII | GATC | 4 cut(s) | 373, 468, 643, 1242 |
| DraIII | CACNNNGTG | 2 cut(s) | 460, 655 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1008 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1008 |
| EaeI | YGGCCR | 2 cut(s) | 88, 685 |
| EagI | CGGCCG | 1 cut(s) | 88 |
| Eam1104I | CTCTTC | 1 cut(s) | 543 |
| EarI | CTCTTC | 1 cut(s) | 543 |
| EciI | GGCGGA | 1 cut(s) | 1133 |
| Ecl136II | GAGCTC | 2 cut(s) | 169, 838 |
| EclXI | CGGCCG | 1 cut(s) | 88 |
| Eco130I | CCWWGG | 1 cut(s) | 150 |
| Eco24I | GRGCYC | 3 cut(s) | 171, 840, 1286 |
| Eco32I | GATATC | 3 cut(s) | 240, 786, 1060 |
| Eco47I | GGWCC | 6 cut(s) | 97, 136, 463, 473, 977, 1051 |
| Eco52I | CGGCCG | 1 cut(s) | 88 |
| Eco53kI | GAGCTC | 2 cut(s) | 169, 838 |
| Eco57I | CTGAAG | 2 cut(s) | 596, 1173 |
| Eco72I | CACGTG | 1 cut(s) | 652 |
| Eco88I | CYCGRG | 2 cut(s) | 63, 701 |
| EcoICRI | GAGCTC | 2 cut(s) | 169, 838 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1046, 1152 |
| EcoO109I | RGGNCCY | 3 cut(s) | 128, 1051, 1283 |
| EcoRII | CCWGG | 3 cut(s) | 131, 1132, 1201 |
| EcoRV | GATATC | 3 cut(s) | 240, 786, 1060 |
| EcoT14I | CCWWGG | 1 cut(s) | 150 |
| EcoT38I | GRGCYC | 3 cut(s) | 171, 840, 1286 |
| ErhI | CCWWGG | 1 cut(s) | 150 |
| Esp3I | CGTCTC | 2 cut(s) | 554, 813 |
| FaeI | CATG | 3 cut(s) | 329, 1078, 1188 |
| FaqI | GGGAC | 4 cut(s) | 149, 449, 665, 1037 |
| FatI | CATG | 3 cut(s) | 325, 1074, 1184 |
| FauI | CCCGC | 4 cut(s) | 344, 640, 676, 824 |
| FblI | GTMKAC | 1 cut(s) | 1159 |
| Fnu4HI | GCNGC | 5 cut(s) | 156, 179, 182, 530, 1176 |
| FokI | GGATG | 3 cut(s) | 317, 359, 486 |
| FriOI | GRGCYC | 3 cut(s) | 171, 840, 1286 |
| Fsp4HI | GCNGC | 5 cut(s) | 156, 179, 182, 530, 1176 |
| FspBI | CTAG | 2 cut(s) | 45, 1259 |
| GlaI | GCGC | 6 cut(s) | 247, 249, 659, 676, 712, 800 |
| GluI | GCNGC | 5 cut(s) | 156, 179, 182, 530, 1176 |
| GsaI | CCCAGC | 1 cut(s) | 1290 |
| HaeII | RGCGCY | 1 cut(s) | 678 |
| HaeIII | GGCC | 8 cut(s) | 69, 83, 90, 129, 155, 439, 687, 1284 |
| HapII | CCGG | 9 cut(s) | 87, 91, 243, 403, 457, 481, 672, 684, 796 |
| HgaI | GACGC | 1 cut(s) | 1011 |
| HhaI | GCGC | 6 cut(s) | 248, 250, 660, 677, 713, 801 |
| Hin1I | GRCGYC | 1 cut(s) | 1003 |
| Hin1II | CATG | 3 cut(s) | 329, 1078, 1188 |
| Hin6I | GCGC | 6 cut(s) | 246, 248, 658, 675, 711, 799 |
| HinP1I | GCGC | 6 cut(s) | 246, 248, 658, 675, 711, 799 |
| HincII | GTYRAC | 3 cut(s) | 523, 814, 855 |
| HindII | GTYRAC | 3 cut(s) | 523, 814, 855 |
| HinfI | GANTC | 3 cut(s) | 555, 706, 1271 |
| HpaII | CCGG | 9 cut(s) | 87, 91, 243, 403, 457, 481, 672, 684, 796 |
| Hpy166II | GTNNAC | 4 cut(s) | 523, 814, 855, 1160 |
| Hpy188I | TCNGA | 9 cut(s) | 144, 213, 228, 297, 473, 554, 604, 643, 1001 |
| Hpy188III | TCNNGA | 8 cut(s) | 331, 371, 466, 841, 877, 912, 972, 1045 |
| Hpy8I | GTNNAC | 4 cut(s) | 523, 814, 855, 1160 |
| HpyAV | CCTTC | 7 cut(s) | 88, 503, 620, 909, 1042, 1077, 1148 |
| HpyCH4III | ACNGT | 4 cut(s) | 232, 736, 766, 981 |
| HpyCH4IV | ACGT | 1 cut(s) | 651 |
| HpyCH4V | TGCA | 4 cut(s) | 4, 117, 325, 1178 |
| HpyF3I | CTNAG | 2 cut(s) | 77, 897 |
| HpySE526I | ACGT | 1 cut(s) | 651 |
| Hsp92I | GRCGYC | 1 cut(s) | 1003 |
| Hsp92II | CATG | 3 cut(s) | 329, 1078, 1188 |
| HspAI | GCGC | 6 cut(s) | 246, 248, 658, 675, 711, 799 |
| KroI | GCCGGC | 1 cut(s) | 671 |
| KroNI | GCCGGC | 1 cut(s) | 673 |
| Kzo9I | GATC | 4 cut(s) | 373, 468, 643, 1242 |
| LmnI | GCTCC | 8 cut(s) | 298, 364, 570, 621, 644, 798, 835, 1219 |
| Lsp1109I | GCAGC | 2 cut(s) | 165, 1162 |
| LweI | GCATC | 2 cut(s) | 780, 1004 |
| MaeI | CTAG | 2 cut(s) | 45, 1259 |
| MaeII | ACGT | 1 cut(s) | 651 |
| MalI | GATC | 4 cut(s) | 375, 470, 645, 1244 |
| MbiI | CCGCTC | 2 cut(s) | 514, 565 |
| MboI | GATC | 4 cut(s) | 373, 468, 643, 1242 |
| MboII | GAAGA | 6 cut(s) | 20, 213, 530, 730, 975, 1237 |
| MhlI | GDGCHC | 4 cut(s) | 171, 633, 840, 1286 |
| MluCI | AATT | 1 cut(s) | 984 |
| MmeI | TCCRAC | 3 cut(s) | 251, 627, 1024 |
| MreI | CGCCGGCG | 1 cut(s) | 671 |
| MroNI | GCCGGC | 1 cut(s) | 671 |
| MseI | TTAA | 1 cut(s) | 1194 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1223 |
| MspA1I | CMGCKG | 4 cut(s) | 529, 586, 635, 1290 |
| MspI | CCGG | 9 cut(s) | 87, 91, 243, 403, 457, 481, 672, 684, 796 |
| MspR9I | CCNGG | 5 cut(s) | 133, 458, 684, 1134, 1203 |
| MvaI | CCWGG | 3 cut(s) | 133, 1134, 1203 |
| MvnI | CGCG | 6 cut(s) | 248, 266, 432, 711, 801, 842 |
| NaeI | GCCGGC | 1 cut(s) | 673 |
| NciI | CCSGG | 2 cut(s) | 458, 684 |
| NdeII | GATC | 4 cut(s) | 373, 468, 643, 1242 |
| NgoMIV | GCCGGC | 1 cut(s) | 671 |
| NlaIII | CATG | 3 cut(s) | 329, 1078, 1188 |
| NruI | TCGCGA | 1 cut(s) | 842 |
| NspI | RCATGY | 1 cut(s) | 329 |
| NspV | TTCGAA | 1 cut(s) | 759 |
| PaeR7I | CTCGAG | 1 cut(s) | 63 |
| PasI | CCCWGGG | 1 cut(s) | 132 |
| PauI | GCGCGC | 1 cut(s) | 246 |
| PcsI | WCGNNNNNNNCGW | 6 cut(s) | 168, 171, 263, 765, 822, 1009 |
| PdiI | GCCGGC | 1 cut(s) | 673 |
| PfeI | GAWTC | 3 cut(s) | 555, 706, 1271 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 460 |
| PfoI | TCCNGGA | 1 cut(s) | 1201 |
| PkrI | GCNGC | 5 cut(s) | 157, 180, 183, 531, 1177 |
| Ple19I | CGATCG | 2 cut(s) | 471, 646 |
| PmaCI | CACGTG | 1 cut(s) | 652 |
| PmlI | CACGTG | 1 cut(s) | 652 |
| Ppu21I | YACGTR | 1 cut(s) | 652 |
| PpuMI | RGGWCCY | 1 cut(s) | 1051 |
| PshAI | GACNNNNGTC | 1 cut(s) | 860 |
| Psp124BI | GAGCTC | 2 cut(s) | 171, 840 |
| Psp5II | RGGWCCY | 1 cut(s) | 1051 |
| Psp6I | CCWGG | 3 cut(s) | 131, 1132, 1201 |
| PspCI | CACGTG | 1 cut(s) | 652 |
| PspFI | CCCAGC | 1 cut(s) | 1286 |
| PspGI | CCWGG | 3 cut(s) | 131, 1132, 1201 |
| PspOMI | GGGCCC | 1 cut(s) | 1282 |
| PspPPI | RGGWCCY | 1 cut(s) | 1051 |
| PspXI | VCTCGAGB | 1 cut(s) | 63 |
| PstI | CTGCAG | 1 cut(s) | 1180 |
| PstNI | CAGNNNCTG | 2 cut(s) | 945, 955 |
| PteI | GCGCGC | 1 cut(s) | 246 |
| PvuI | CGATCG | 2 cut(s) | 471, 646 |
| PvuII | CAGCTG | 2 cut(s) | 586, 1290 |
| RruI | TCGCGA | 1 cut(s) | 842 |
| RsaI | GTAC | 1 cut(s) | 774 |
| RsaNI | GTAC | 1 cut(s) | 773 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1223 |
| SacI | GAGCTC | 2 cut(s) | 171, 840 |
| SaqAI | TTAA | 1 cut(s) | 1194 |
| SatI | GCNGC | 5 cut(s) | 156, 179, 182, 530, 1176 |
| Sau3AI | GATC | 4 cut(s) | 373, 468, 643, 1242 |
| ScrFI | CCNGG | 5 cut(s) | 133, 458, 684, 1134, 1203 |
| SduI | GDGCHC | 4 cut(s) | 171, 633, 840, 1286 |
| SfaNI | GCATC | 2 cut(s) | 780, 1004 |
| SfcI | CTRYAG | 1 cut(s) | 1176 |
| Sfr274I | CTCGAG | 1 cut(s) | 63 |
| SfuI | TTCGAA | 1 cut(s) | 759 |
| SgrAI | CRCCGGYG | 1 cut(s) | 671 |
| SinI | GGWCC | 6 cut(s) | 97, 136, 463, 473, 977, 1051 |
| SlaI | CTCGAG | 1 cut(s) | 63 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1223 |
| SmlI | CTYRAG | 3 cut(s) | 63, 749, 970 |
| SmoI | CTYRAG | 3 cut(s) | 63, 749, 970 |
| Sse9I | AATT | 1 cut(s) | 984 |
| SspMI | CTAG | 2 cut(s) | 45, 1259 |
| SstI | GAGCTC | 2 cut(s) | 171, 840 |
| StyD4I | CCNGG | 5 cut(s) | 131, 456, 682, 1132, 1201 |
| StyI | CCWWGG | 1 cut(s) | 150 |
| TaaI | ACNGT | 4 cut(s) | 232, 736, 766, 981 |
| TaiI | ACGT | 1 cut(s) | 654 |
| TaqI | TCGA | 8 cut(s) | 64, 162, 171, 717, 741, 759, 788, 1012 |
| TaqII | GACCGA | 1 cut(s) | 920 |
| TasI | AATT | 1 cut(s) | 984 |
| TauI | GCSGC | 3 cut(s) | 158, 184, 532 |
| TfiI | GAWTC | 3 cut(s) | 555, 706, 1271 |
| Tru1I | TTAA | 1 cut(s) | 1194 |
| Tru9I | TTAA | 1 cut(s) | 1194 |
| TscAI | CASTG | 2 cut(s) | 237, 702 |
| TseI | GCWGC | 2 cut(s) | 178, 1175 |
| TspDTI | ATGAA | 2 cut(s) | 759, 1063 |
| TspGWI | ACGGA | 4 cut(s) | 354, 548, 796, 831 |
| TspRI | CASTG | 2 cut(s) | 237, 702 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 460 |
| VpaK11BI | GGWCC | 6 cut(s) | 97, 136, 463, 473, 977, 1051 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1046, 1152 |
| XceI | RCATGY | 1 cut(s) | 329 |
| XhoI | CTCGAG | 1 cut(s) | 63 |
| XmiI | GTMKAC | 1 cut(s) | 1159 |
| XspI | CTAG | 2 cut(s) | 45, 1259 |