RchiOBHm_Chr6g0249251

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000686
Physical Location & Seq
Reverse (-)
5137579 .. 5138444
866 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22346

Sequence Viewer

Length: 531 bp
ATGGGGCAACGTGTTGATGTTGCTGATGTGCAGTCTGAACCAAGTTCTGCTCCGGTTGCACCGATTAATGTTAGGAATGAGATTGCTGAGGAAGTAACTGCAAATGTAGGTGATCTGAAGCTAACAGATGATGATTCCGCTGATCAGTCGACTGGGGAGGATCAACAACCTCTTTCTGCTGAGGATGTTGACATGCTTTTGGAAAAATGCCTCCTGCAAGCATTGCATACAACTGTCAAAGACAAAGACCTTCCCATGCCTGGAAGCACTCTATGGTCAAACTATGTTTTACCTTGTGGTTCTTCAGGCATCACACTAGACATCAAGAAATCATCATACAAAAAATTATCAAAGTGGTTGCAAGCTAAATGTTCTACAGGATTGATACGTTATGCTTTAATTTTCCTCTTTCTCATAAGTGACCACCTTTTCAGTTTTAACCTTGTCCTTTTGAGGGTTATCTATATTGGTATTTTTGTAGTTTTGATTCTGAACACATTCCGCACATCCTTATGGTGTTGTCAGCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SUI1

Protein Analysis

176

Amino Acids

19.54

Weight (kDa)

4.97

Isoelectric Point (pI)

39.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_eIF2D PF25304 64 - 131 3.3e-24 eIF2D winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000686)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71350
fragaria_vesca FvH4_2g00430 FvH4_2g00430 FvH4_2g00430 FvH4_2g00430
malus_domestica MD10G1002600.v1.1
prunus_persica Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0249251 RchiOBHm_Chr6g0249261 RchiOBHm_Chr6g0249701 RchiOBHm_Chr6g0249761 RchiOBHm_Chr6g0249851 RchiOBHm_Chr6g0249861 RchiOBHm_Chr6g0249871 RchiOBHm_Chr6g0249891 RchiOBHm_Chr6g0249901 RchiOBHm_Chr6g0249911 RchiOBHm_Chr6g0249981 RchiOBHm_Chr6g0249991 RchiOBHm_Chr6g0250001 RchiOBHm_Chr6g0250151 RchiOBHm_Chr6g0250171 RchiOBHm_Chr6g0250191 RchiOBHm_Chr6g0250211 RchiOBHm_Chr6g0250221 RchiOBHm_Chr6g0250321 RchiOBHm_Chr6g0250391
rosa_laevigata RLG00000015543 RLG00000015548
rosa_multiflora Rmu_sc0000411.1_g000004 Rmu_sc0000411.1_g000009 Rmu_sc0000704.1_g000007 Rmu_sc0000704.1_g000011 Rmu_sc0001209.1_g000038 Rmu_sc0001209.1_g000042 Rmu_sc0001209.1_g000048 Rmu_sc0003293.1_g000005 Rmu_sc0004691.1_g000005 Rmu_sc0004691.1_g000007 Rmu_sc0004691.1_g000009 Rmu_sc0006032.1_g000001 Rmu_sc0006032.1_g000004 Rmu_sc0006032.1_g000005 Rmu_sc0008209.1_g000003 Rmu_sc0008209.1_g000010 Rmu_sc0009002.1_g000001 Rmu_sc0009002.1_g000005 Rmu_sc0009002.1_g000009
rosa_roxburghii Rroxscaffold_3G00218110 Rroxscaffold_7G00217270 Rroxscaffold_7G00217290 Rroxscaffold_7G00217300 Rroxscaffold_7G00217340
rosa_rugosa Rorug05G0494700 Rorug05G0495000 Rorug05G0496000 Rorug05G0498100 Rorug05G0498200
rosa_samantha Rh6AG000200 Rh6AG000300 Rh6AG004700 Rh6AG004800 Rh6AG005200 Rh6AG005300 Rh6AG005600 Rh6BG005500 Rh6BG005600 Rh6CG003700 Rh6DG003600
rosa_wichuraiana Rw6G000560 Rw6G000600 Rw6G000620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 149
AciI CCGC 3 cut(s) 138, 502, 526
AclWI GGATC 1 cut(s) 168
AcuI CTGAAG 2 cut(s) 137, 288
AfiI CCNNNNNNNGG 2 cut(s) 260, 454
AflIII ACRYGT 1 cut(s) 10
AjnI CCWGG 1 cut(s) 259
AluBI AGCT 2 cut(s) 121, 365
AluI AGCT 2 cut(s) 121, 365
AlwI GGATC 1 cut(s) 168
AseI ATTAAT 1 cut(s) 66
Asp700I GAANNNNTTC 1 cut(s) 497
AsuHPI GGTGA 1 cut(s) 122
BbvCI CCTCAGC 2 cut(s) 87, 180
BciT130I CCWGG 1 cut(s) 261
BclI TGATCA 1 cut(s) 142
BfaI CTAG 1 cut(s) 317
BfmI CTRYAG 1 cut(s) 375
Bme1390I CCNGG 1 cut(s) 261
BmrFI CCNGG 1 cut(s) 261
BmrI ACTGGG 1 cut(s) 162
BmsI GCATC 1 cut(s) 318
BmuI ACTGGG 1 cut(s) 162
Bpu10I CCTNAGC 2 cut(s) 87, 180
BsaWI WCCGGW 1 cut(s) 52
Bsc4I CCNNNNNNNGG 2 cut(s) 260, 454
Bse1I ACTGG 1 cut(s) 157
Bse3DI GCAATG 1 cut(s) 221
BseBI CCWGG 1 cut(s) 261
BseGI GGATG 2 cut(s) 190, 506
BseLI CCNNNNNNNGG 2 cut(s) 260, 454
BseMI GCAATG 1 cut(s) 221
BseMII CTCAG 2 cut(s) 78, 171
BseNI ACTGG 1 cut(s) 157
BsgI GTGCAG 1 cut(s) 50
BsiSI CCGG 1 cut(s) 53
BslI CCNNNNNNNGG 2 cut(s) 260, 454
Bsp143I GATC 3 cut(s) 112, 142, 160
BspACI CCGC 3 cut(s) 138, 502, 526
BspCNI CTCAG 2 cut(s) 79, 172
BspPI GGATC 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 221
BsrI ACTGG 1 cut(s) 157
BssMI GATC 3 cut(s) 112, 142, 160
Bst2UI CCWGG 1 cut(s) 261
Bst4CI ACNGT 1 cut(s) 235
BstAPI GCANNNNNTGC 1 cut(s) 223
BstC8I GCNNGC 2 cut(s) 219, 363
BstDEI CTNAG 2 cut(s) 87, 180
BstF5I GGATG 2 cut(s) 190, 506
BstKTI GATC 3 cut(s) 115, 145, 163
BstMBI GATC 3 cut(s) 112, 142, 160
BstMWI GCNNNNNNNGC 2 cut(s) 56, 223
BstNI CCWGG 1 cut(s) 261
BstNSI RCATGY 1 cut(s) 196
BstSCI CCNGG 1 cut(s) 259
BstSFI CTRYAG 1 cut(s) 375
BtsCI GGATG 2 cut(s) 190, 506
Cac8I GCNNGC 2 cut(s) 219, 363
CviAII CATG 2 cut(s) 193, 256
CviJI RGCY 2 cut(s) 121, 365
CviKI_1 RGCY 2 cut(s) 121, 365
DdeI CTNAG 2 cut(s) 87, 180
DpnI GATC 3 cut(s) 114, 144, 162
DpnII GATC 3 cut(s) 112, 142, 160
Eco57I CTGAAG 2 cut(s) 137, 288
EcoRII CCWGG 1 cut(s) 259
FaeI CATG 2 cut(s) 196, 259
FatI CATG 2 cut(s) 192, 255
FbaI TGATCA 1 cut(s) 142
FblI GTMKAC 1 cut(s) 149
FokI GGATG 2 cut(s) 197, 493
FspBI CTAG 1 cut(s) 317
HapII CCGG 1 cut(s) 53
Hin1II CATG 2 cut(s) 196, 259
HincII GTYRAC 2 cut(s) 150, 190
HindII GTYRAC 2 cut(s) 150, 190
HinfI GANTC 2 cut(s) 134, 487
HpaII CCGG 1 cut(s) 53
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 2 cut(s) 150, 190
Hpy188I TCNGA 3 cut(s) 37, 117, 492
Hpy188III TCNNGA 1 cut(s) 325
Hpy8I GTNNAC 2 cut(s) 150, 190
HpyAV CCTTC 1 cut(s) 260
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4IV ACGT 2 cut(s) 10, 388
HpyCH4V TGCA 6 cut(s) 31, 59, 101, 217, 226, 361
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 223
HpyF3I CTNAG 2 cut(s) 87, 180
HpySE526I ACGT 2 cut(s) 10, 388
Hsp92II CATG 2 cut(s) 196, 259
Ksp22I TGATCA 1 cut(s) 142
Kzo9I GATC 3 cut(s) 112, 142, 160
LmnI GCTCC 1 cut(s) 55
LpnPI CCDG 7 cut(s) 66, 138, 227, 246, 273, 291, 363
LweI GCATC 1 cut(s) 318
MaeI CTAG 1 cut(s) 317
MaeII ACGT 2 cut(s) 10, 388
MaeIII GTNAC 2 cut(s) 94, 419
MalI GATC 3 cut(s) 114, 144, 162
MboI GATC 3 cut(s) 112, 142, 160
MboII GAAGA 1 cut(s) 294
MluCI AATT 2 cut(s) 344, 399
MnlI CCTC 7 cut(s) 82, 151, 175, 180, 221, 416, 447
MroXI GAANNNNTTC 1 cut(s) 497
MseI TTAA 3 cut(s) 66, 398, 438
MslI CAYNNNNRTG 1 cut(s) 511
MspA1I CMGCKG 2 cut(s) 140, 526
MspI CCGG 1 cut(s) 53
MspR9I CCNGG 1 cut(s) 261
MvaI CCWGG 1 cut(s) 261
MwoI GCNNNNNNNGC 2 cut(s) 56, 223
NdeII GATC 3 cut(s) 112, 142, 160
NlaIII CATG 2 cut(s) 196, 259
NmuCI GTSAC 1 cut(s) 419
NspI RCATGY 1 cut(s) 196
PdmI GAANNNNTTC 1 cut(s) 497
PfeI GAWTC 2 cut(s) 134, 487
PshBI ATTAAT 1 cut(s) 66
Psp6I CCWGG 1 cut(s) 259
PspGI CCWGG 1 cut(s) 259
RseI CAYNNNNRTG 1 cut(s) 511
SalI GTCGAC 1 cut(s) 148
SaqAI TTAA 3 cut(s) 66, 398, 438
Sau3AI GATC 3 cut(s) 112, 142, 160
ScrFI CCNGG 1 cut(s) 261
SfaNI GCATC 1 cut(s) 318
SfcI CTRYAG 1 cut(s) 375
SmiMI CAYNNNNRTG 1 cut(s) 511
Sse9I AATT 2 cut(s) 344, 399
SsiI CCGC 3 cut(s) 138, 502, 526
SspMI CTAG 1 cut(s) 317
StyD4I CCNGG 1 cut(s) 259
TaaI ACNGT 1 cut(s) 235
TaiI ACGT 2 cut(s) 13, 391
TaqI TCGA 1 cut(s) 149
TasI AATT 2 cut(s) 344, 399
TfiI GAWTC 2 cut(s) 134, 487
Tru1I TTAA 3 cut(s) 66, 398, 438
Tru9I TTAA 3 cut(s) 66, 398, 438
TseFI GTSAC 1 cut(s) 419
Tsp45I GTSAC 1 cut(s) 419
VspI ATTAAT 1 cut(s) 66
XceI RCATGY 1 cut(s) 196
XmiI GTMKAC 1 cut(s) 149
XmnI GAANNNNTTC 1 cut(s) 497
XspI CTAG 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.