Rroxscaffold_7G00217290

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000686
Physical Location & Seq
Forward (+)
67767463 .. 67771321
3859 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00217290.1

Sequence Viewer

Length: 861 bp
ATGCTTTTGGAAAAATGCCTTTTACAAGCATTGCGTACAACTGTCAAGGACAAAGACCTTCCCATGCCCGGAAGCACTCTATGGTCAAACTATGTTTTACCTTGTAGGCCTTCAGGCATCACACTAGACATCAAGAAATCATCATACAAGAAATTATCAAAGTGGTTACAAGCTAAATGTTCTAAAGGATTGATTAGAGTGAAGGAAGATAAATATAAGGAGTCTGTATTACTTTCTGTTAACCGCAGCCATCCAGATTACTCATCTTTCAAGCCAGAGAAACGACAAGTTGAAGAAGCTGTTCAAACTGGTGTTCCTGCTGTCAGTGAAAGTCGGTCGGTCAAAATCCTTGAAGTGGCTGAGATCTATAAACCAAGTGTACAAGTAAATCCAGTATTTGCTTCTGTAGGTGCTGACACAGGCGAGCTTTACAGTGCTTCAGATGCCACTGACATTGTGTTTAAATATGTTGAAAAGGAAAACCTGGTTAAGCCGACAGATAAATCCATTGTGATTTTGGATGCGATATTGTGTGATGCACTGTTCAAGGGGGCCATAAAGAAAGGTTCAATATACCCTACTGAAATCCATAAAAGAGACTTGGGAGCAGCATTTGTGATCCGGATGCAAGCCCATCACATTGTGACAAGGGGAAATGACTCGGTTGTTCGTAAAGGTGGTTTGAAAACAGTCCAGATAATGACAGAAAGGCGGCATGGTAATAAGAAAATGACCAAGTTATCAGGACTGGAAACATTTTTAGTGGATCCTGAAGCTTTGGCATCAGAGCTGCAAAAGAAGTTCGCTTGCAGTACAACGGTGGCAGAACTACCAGGCGTGTGGCACAAATATGCACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SUI1

Protein Analysis

286

Amino Acids

31.85

Weight (kDa)

9.45

Isoelectric Point (pI)

41.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_eIF2D PF25304 2 - 81 2.9e-29 eIF2D winged helix domain
SWIB_eIF2D PF26291 123 - 183 6.5e-13 eIF2D, SWIB domain
SUI1 PF01253 229 - 276 1.9e-14 Translation initiation factor SUI1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000686)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71350
fragaria_vesca FvH4_2g00430 FvH4_2g00430 FvH4_2g00430 FvH4_2g00430
malus_domestica MD10G1002600.v1.1
prunus_persica Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0249251 RchiOBHm_Chr6g0249261 RchiOBHm_Chr6g0249701 RchiOBHm_Chr6g0249761 RchiOBHm_Chr6g0249851 RchiOBHm_Chr6g0249861 RchiOBHm_Chr6g0249871 RchiOBHm_Chr6g0249891 RchiOBHm_Chr6g0249901 RchiOBHm_Chr6g0249911 RchiOBHm_Chr6g0249981 RchiOBHm_Chr6g0249991 RchiOBHm_Chr6g0250001 RchiOBHm_Chr6g0250151 RchiOBHm_Chr6g0250171 RchiOBHm_Chr6g0250191 RchiOBHm_Chr6g0250211 RchiOBHm_Chr6g0250221 RchiOBHm_Chr6g0250321 RchiOBHm_Chr6g0250391
rosa_laevigata RLG00000015543 RLG00000015548
rosa_multiflora Rmu_sc0000411.1_g000004 Rmu_sc0000411.1_g000009 Rmu_sc0000704.1_g000007 Rmu_sc0000704.1_g000011 Rmu_sc0001209.1_g000038 Rmu_sc0001209.1_g000042 Rmu_sc0001209.1_g000048 Rmu_sc0003293.1_g000005 Rmu_sc0004691.1_g000005 Rmu_sc0004691.1_g000007 Rmu_sc0004691.1_g000009 Rmu_sc0006032.1_g000001 Rmu_sc0006032.1_g000004 Rmu_sc0006032.1_g000005 Rmu_sc0008209.1_g000003 Rmu_sc0008209.1_g000010 Rmu_sc0009002.1_g000001 Rmu_sc0009002.1_g000005 Rmu_sc0009002.1_g000009
rosa_roxburghii Rroxscaffold_3G00218110 Rroxscaffold_7G00217270 Rroxscaffold_7G00217290 Rroxscaffold_7G00217300 Rroxscaffold_7G00217340
rosa_rugosa Rorug05G0494700 Rorug05G0495000 Rorug05G0496000 Rorug05G0498100 Rorug05G0498200
rosa_samantha Rh6AG000200 Rh6AG000300 Rh6AG004700 Rh6AG004800 Rh6AG005200 Rh6AG005300 Rh6AG005600 Rh6BG005500 Rh6BG005600 Rh6CG003700 Rh6DG003600
rosa_wichuraiana Rw6G000560 Rw6G000600 Rw6G000620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 621
AciI CCGC 2 cut(s) 244, 712
AclWI GGATC 3 cut(s) 613, 761, 774
AcuI CTGAAG 3 cut(s) 96, 423, 792
AdeI CACNNNGTG 1 cut(s) 643
AfaI GTAC 3 cut(s) 37, 381, 814
AfiI CCNNNNNNNGG 2 cut(s) 68, 355
AgsI TTSAA 8 cut(s) 271, 293, 305, 353, 473, 547, 570, 685
AjnI CCWGG 2 cut(s) 483, 832
AluBI AGCT 5 cut(s) 173, 299, 427, 776, 790
AluI AGCT 5 cut(s) 173, 299, 427, 776, 790
Alw26I GTCTC 1 cut(s) 591
AlwI GGATC 3 cut(s) 613, 761, 774
Aor13HI TCCGGA 1 cut(s) 621
AoxI GGCC 2 cut(s) 107, 552
ApeKI GCWGC 3 cut(s) 246, 608, 790
Asp700I GAANNNNTTC 1 cut(s) 300
AspS9I GGNCC 1 cut(s) 552
AsuC2I CCSGG 1 cut(s) 69
BamHI GGATCC 1 cut(s) 766
BbvI GCAGC 3 cut(s) 258, 620, 777
BccI CCATC 2 cut(s) 258, 642
BciT130I CCWGG 2 cut(s) 485, 834
BcnI CCSGG 1 cut(s) 69
BcoDI GTCTC 1 cut(s) 591
BfaI CTAG 1 cut(s) 125
BfmI CTRYAG 1 cut(s) 405
BglII AGATCT 1 cut(s) 363
BisI GCNGC 4 cut(s) 247, 609, 713, 791
BlsI GCNGC 4 cut(s) 248, 610, 714, 792
Bme1390I CCNGG 3 cut(s) 69, 485, 834
BmgT120I GGNCC 1 cut(s) 552
BmiI GGNNCC 2 cut(s) 553, 768
BmrFI CCNGG 3 cut(s) 69, 485, 834
BmsI GCATC 6 cut(s) 126, 433, 511, 526, 615, 791
BpuMI CCSGG 1 cut(s) 69
BsaWI WCCGGW 1 cut(s) 621
Bsc4I CCNNNNNNNGG 2 cut(s) 68, 355
Bse1I ACTGG 3 cut(s) 313, 392, 753
Bse3DI GCAATG 1 cut(s) 29
BseAI TCCGGA 1 cut(s) 621
BseBI CCWGG 2 cut(s) 485, 834
BseGI GGATG 3 cut(s) 250, 526, 630
BseLI CCNNNNNNNGG 2 cut(s) 68, 355
BseMI GCAATG 1 cut(s) 29
BseMII CTCAG 1 cut(s) 351
BseNI ACTGG 3 cut(s) 313, 392, 753
BseXI GCAGC 3 cut(s) 258, 620, 777
Bsh1285I CGRYCG 1 cut(s) 338
BshFI GGCC 2 cut(s) 109, 554
BsiEI CGRYCG 1 cut(s) 338
BsiSI CCGG 2 cut(s) 69, 622
BslI CCNNNNNNNGG 2 cut(s) 68, 355
BsmAI GTCTC 1 cut(s) 591
BsnI GGCC 2 cut(s) 109, 554
Bsp13I TCCGGA 1 cut(s) 621
Bsp1407I TGTACA 1 cut(s) 379
Bsp143I GATC 3 cut(s) 363, 618, 766
BspACI CCGC 2 cut(s) 244, 712
BspANI GGCC 2 cut(s) 109, 554
BspCNI CTCAG 1 cut(s) 352
BspEI TCCGGA 1 cut(s) 621
BspLI GGNNCC 2 cut(s) 553, 768
BspPI GGATC 3 cut(s) 613, 761, 774
BsrDI GCAATG 1 cut(s) 29
BsrGI TGTACA 1 cut(s) 379
BsrI ACTGG 3 cut(s) 313, 392, 753
BssMI GATC 3 cut(s) 363, 618, 766
Bst2UI CCWGG 2 cut(s) 485, 834
Bst4CI ACNGT 6 cut(s) 43, 434, 543, 691, 820, 858
BstAUI TGTACA 1 cut(s) 379
BstC8I GCNNGC 3 cut(s) 425, 630, 808
BstDEI CTNAG 1 cut(s) 360
BstF5I GGATG 3 cut(s) 250, 526, 630
BstKTI GATC 3 cut(s) 366, 621, 769
BstMAI GTCTC 1 cut(s) 591
BstMBI GATC 3 cut(s) 363, 618, 766
BstMCI CGRYCG 1 cut(s) 338
BstMWI GCNNNNNNNGC 1 cut(s) 443
BstNI CCWGG 2 cut(s) 485, 834
BstSCI CCNGG 3 cut(s) 67, 483, 832
BstSFI CTRYAG 1 cut(s) 405
BstV1I GCAGC 3 cut(s) 258, 620, 777
BstX2I RGATCY 2 cut(s) 363, 766
BstXI CCANNNNNNTGG 1 cut(s) 840
BstYI RGATCY 2 cut(s) 363, 766
BsuRI GGCC 2 cut(s) 109, 554
BtsCI GGATG 3 cut(s) 250, 526, 630
BtsIMutI CAGTG 4 cut(s) 331, 439, 447, 539
Cac8I GCNNGC 3 cut(s) 425, 630, 808
Cfr13I GGNCC 1 cut(s) 552
CsiI ACCWGGT 1 cut(s) 483
Csp6I GTAC 3 cut(s) 36, 380, 813
CspCI CAANNNNNGTGG 2 cut(s) 436, 471
CviAII CATG 2 cut(s) 64, 716
CviQI GTAC 3 cut(s) 36, 380, 813
DdeI CTNAG 1 cut(s) 360
DpnI GATC 3 cut(s) 365, 620, 768
DpnII GATC 3 cut(s) 363, 618, 766
DraI TTTAAA 1 cut(s) 463
DraIII CACNNNGTG 1 cut(s) 643
Eco147I AGGCCT 1 cut(s) 109
Eco57I CTGAAG 3 cut(s) 96, 423, 792
EcoRII CCWGG 2 cut(s) 483, 832
FaeI CATG 2 cut(s) 67, 719
FatI CATG 2 cut(s) 63, 715
Fnu4HI GCNGC 4 cut(s) 247, 609, 713, 791
FokI GGATG 3 cut(s) 237, 533, 637
Fsp4HI GCNGC 4 cut(s) 247, 609, 713, 791
FspBI CTAG 1 cut(s) 125
GluI GCNGC 4 cut(s) 247, 609, 713, 791
HaeIII GGCC 2 cut(s) 109, 554
HapII CCGG 2 cut(s) 69, 622
Hin1II CATG 2 cut(s) 67, 719
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HindIII AAGCTT 1 cut(s) 774
HinfI GANTC 2 cut(s) 221, 659
HpaI GTTAAC 1 cut(s) 241
HpaII CCGG 2 cut(s) 69, 622
Hpy166II GTNNAC 2 cut(s) 241, 380
Hpy188I TCNGA 2 cut(s) 442, 787
Hpy188III TCNNGA 6 cut(s) 133, 254, 622, 694, 744, 770
Hpy8I GTNNAC 2 cut(s) 241, 380
HpyAV CCTTC 3 cut(s) 68, 120, 196
HpyCH4III ACNGT 6 cut(s) 43, 434, 543, 691, 820, 858
HpyCH4V TGCA 5 cut(s) 539, 628, 793, 810, 854
HpyF10VI GCNNNNNNNGC 1 cut(s) 443
HpyF3I CTNAG 1 cut(s) 360
Hsp92II CATG 2 cut(s) 67, 719
Kpn2I TCCGGA 1 cut(s) 621
KspAI GTTAAC 1 cut(s) 241
Kzo9I GATC 3 cut(s) 363, 618, 766
LmnI GCTCC 1 cut(s) 605
Lsp1109I GCAGC 3 cut(s) 258, 620, 777
LweI GCATC 6 cut(s) 126, 433, 511, 526, 615, 791
MabI ACCWGGT 1 cut(s) 483
MaeI CTAG 1 cut(s) 125
MaeIII GTNAC 2 cut(s) 165, 643
MalI GATC 3 cut(s) 365, 620, 768
MboI GATC 3 cut(s) 363, 618, 766
MboII GAAGA 2 cut(s) 218, 305
MflI RGATCY 2 cut(s) 363, 766
MluCI AATT 1 cut(s) 152
MlyI GAGTC 2 cut(s) 230, 653
MroI TCCGGA 1 cut(s) 621
MroXI GAANNNNTTC 1 cut(s) 300
MseI TTAA 3 cut(s) 240, 462, 489
MslI CAYNNNNRTG 1 cut(s) 849
MspI CCGG 2 cut(s) 69, 622
MspR9I CCNGG 3 cut(s) 69, 485, 834
MvaI CCWGG 2 cut(s) 485, 834
MwoI GCNNNNNNNGC 1 cut(s) 443
NciI CCSGG 1 cut(s) 69
NdeII GATC 3 cut(s) 363, 618, 766
NlaIII CATG 2 cut(s) 67, 719
NlaIV GGNNCC 2 cut(s) 553, 768
NmuCI GTSAC 1 cut(s) 643
PceI AGGCCT 1 cut(s) 109
PdmI GAANNNNTTC 1 cut(s) 300
PkrI GCNGC 4 cut(s) 248, 610, 714, 792
PleI GAGTC 2 cut(s) 229, 653
PpsI GAGTC 2 cut(s) 229, 653
Psp6I CCWGG 2 cut(s) 483, 832
PspGI CCWGG 2 cut(s) 483, 832
PspN4I GGNNCC 2 cut(s) 553, 768
PspPI GGNCC 1 cut(s) 552
PsuI RGATCY 2 cut(s) 363, 766
RsaI GTAC 3 cut(s) 37, 381, 814
RsaNI GTAC 3 cut(s) 36, 380, 813
RseI CAYNNNNRTG 1 cut(s) 849
SaqAI TTAA 3 cut(s) 240, 462, 489
SatI GCNGC 4 cut(s) 247, 609, 713, 791
Sau3AI GATC 3 cut(s) 363, 618, 766
Sau96I GGNCC 1 cut(s) 552
SchI GAGTC 2 cut(s) 230, 653
ScrFI CCNGG 3 cut(s) 69, 485, 834
SexAI ACCWGGT 1 cut(s) 483
SfaNI GCATC 6 cut(s) 126, 433, 511, 526, 615, 791
SfcI CTRYAG 1 cut(s) 405
SmiMI CAYNNNNRTG 1 cut(s) 849
Sse9I AATT 1 cut(s) 152
SseBI AGGCCT 1 cut(s) 109
SsiI CCGC 2 cut(s) 244, 712
SspMI CTAG 1 cut(s) 125
StuI AGGCCT 1 cut(s) 109
StyD4I CCNGG 3 cut(s) 67, 483, 832
TaaI ACNGT 6 cut(s) 43, 434, 543, 691, 820, 858
TaqII GACCGA 2 cut(s) 324, 328
TasI AATT 1 cut(s) 152
TatI WGTACW 2 cut(s) 379, 812
TauI GCSGC 1 cut(s) 715
Tru1I TTAA 3 cut(s) 240, 462, 489
Tru9I TTAA 3 cut(s) 240, 462, 489
TscAI CASTG 4 cut(s) 331, 439, 454, 546
TseFI GTSAC 1 cut(s) 643
TseI GCWGC 3 cut(s) 246, 608, 790
Tsp45I GTSAC 1 cut(s) 643
TspRI CASTG 4 cut(s) 331, 439, 454, 546
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XmnI GAANNNNTTC 1 cut(s) 300
XspI CTAG 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.