RchiOBHm_Chr6g0249991

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000686
Physical Location & Seq
Reverse (-)
5687414 .. 5688239
826 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22410

Sequence Viewer

Length: 612 bp
ATGGGGAAACATGTTGATGTTGCTGATGTGCAGTCTGAACCAAGTTCTGCTTCGGTTGCACCGATTGATGTTAGGAATGAGATTGCTGAGGAAGTAACTGCAAATGTAGGTGATCTGAAGCTAACAGATGATGATTCTGCTGATCAGTCGACTGGGGAGGATCAATATCCTCTTTCTGCTGAGGATGTGGACATGCTTTTGGAAAAATGCCTTTTGCAAGCATTGCATACAACTGTCAAAGACAAAGACCTTCTCATGCCTGGAAGCACTCTATGGCCCTCAGGCATCACACTAGACATCAAGAAATCATCATACAAGAAATTATCAAAGTGGTTGCAAGCTAAATGTTCTACAGGATTGTTGCGTTATGCTTTCATTTTCCTCTTTCTCATAAGTGACCACTTTTTCAGTTTTAACCTAGTCATTTTGAGGGTTATCTATATTGGTATTTTGGTAGTTTTGATTCTGAACACATTCCGCACATCCTTATGGTGTTGTCAGCCATCAGCACCTTTATGTATTAATGCATGGTTTTGTTGCTCTGTATCCTTGACAATATTTGAAAATTTCATTTTTCTTTTATATTTCTACCAATTACTGATCAAGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SUI1

Protein Analysis

203

Amino Acids

22.83

Weight (kDa)

4.87

Isoelectric Point (pI)

35.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_eIF2D PF25304 64 - 122 9.3e-13 eIF2D winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000686)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71350
fragaria_vesca FvH4_2g00430 FvH4_2g00430 FvH4_2g00430 FvH4_2g00430
malus_domestica MD10G1002600.v1.1
prunus_persica Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0249251 RchiOBHm_Chr6g0249261 RchiOBHm_Chr6g0249701 RchiOBHm_Chr6g0249761 RchiOBHm_Chr6g0249851 RchiOBHm_Chr6g0249861 RchiOBHm_Chr6g0249871 RchiOBHm_Chr6g0249891 RchiOBHm_Chr6g0249901 RchiOBHm_Chr6g0249911 RchiOBHm_Chr6g0249981 RchiOBHm_Chr6g0249991 RchiOBHm_Chr6g0250001 RchiOBHm_Chr6g0250151 RchiOBHm_Chr6g0250171 RchiOBHm_Chr6g0250191 RchiOBHm_Chr6g0250211 RchiOBHm_Chr6g0250221 RchiOBHm_Chr6g0250321 RchiOBHm_Chr6g0250391
rosa_laevigata RLG00000015543 RLG00000015548
rosa_multiflora Rmu_sc0000411.1_g000004 Rmu_sc0000411.1_g000009 Rmu_sc0000704.1_g000007 Rmu_sc0000704.1_g000011 Rmu_sc0001209.1_g000038 Rmu_sc0001209.1_g000042 Rmu_sc0001209.1_g000048 Rmu_sc0003293.1_g000005 Rmu_sc0004691.1_g000005 Rmu_sc0004691.1_g000007 Rmu_sc0004691.1_g000009 Rmu_sc0006032.1_g000001 Rmu_sc0006032.1_g000004 Rmu_sc0006032.1_g000005 Rmu_sc0008209.1_g000003 Rmu_sc0008209.1_g000010 Rmu_sc0009002.1_g000001 Rmu_sc0009002.1_g000005 Rmu_sc0009002.1_g000009
rosa_roxburghii Rroxscaffold_3G00218110 Rroxscaffold_7G00217270 Rroxscaffold_7G00217290 Rroxscaffold_7G00217300 Rroxscaffold_7G00217340
rosa_rugosa Rorug05G0494700 Rorug05G0495000 Rorug05G0496000 Rorug05G0498100 Rorug05G0498200
rosa_samantha Rh6AG000200 Rh6AG000300 Rh6AG004700 Rh6AG004800 Rh6AG005200 Rh6AG005300 Rh6AG005600 Rh6BG005500 Rh6BG005600 Rh6CG003700 Rh6DG003600
rosa_wichuraiana Rw6G000560 Rw6G000600 Rw6G000620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 149
AciI CCGC 1 cut(s) 478
AclWI GGATC 1 cut(s) 168
AcsI RAATTY 1 cut(s) 565
AcuI CTGAAG 1 cut(s) 137
AflIII ACRYGT 1 cut(s) 10
AgsI TTSAA 1 cut(s) 563
AjnI CCWGG 1 cut(s) 259
AjuI GAANNNNNNNTTGG 2 cut(s) 34, 66
AluBI AGCT 2 cut(s) 121, 341
AluI AGCT 2 cut(s) 121, 341
AlwI GGATC 1 cut(s) 168
AoxI GGCC 1 cut(s) 275
ApoI RAATTY 1 cut(s) 565
AseI ATTAAT 1 cut(s) 522
Asp700I GAANNNNTTC 1 cut(s) 473
AspS9I GGNCC 1 cut(s) 276
AsuHPI GGTGA 1 cut(s) 122
AxyI CCTNAGG 1 cut(s) 280
BbvCI CCTCAGC 2 cut(s) 87, 180
BccI CCATC 1 cut(s) 511
BciT130I CCWGG 1 cut(s) 261
BciVI GTATCC 1 cut(s) 556
BclI TGATCA 2 cut(s) 142, 600
BfaI CTAG 2 cut(s) 293, 419
BfmI CTRYAG 1 cut(s) 351
BfuI GTATCC 1 cut(s) 556
Bme1390I CCNGG 1 cut(s) 261
BmgT120I GGNCC 1 cut(s) 276
BmrFI CCNGG 1 cut(s) 261
BmrI ACTGGG 1 cut(s) 162
BmsI GCATC 1 cut(s) 294
BmuI ACTGGG 1 cut(s) 162
Bpu10I CCTNAGC 2 cut(s) 87, 180
BsaBI GATNNNNATC 1 cut(s) 165
Bse1I ACTGG 1 cut(s) 157
Bse21I CCTNAGG 1 cut(s) 280
Bse3DI GCAATG 1 cut(s) 221
Bse8I GATNNNNATC 1 cut(s) 165
BseBI CCWGG 1 cut(s) 261
BseGI GGATG 2 cut(s) 190, 482
BseJI GATNNNNATC 1 cut(s) 165
BseMI GCAATG 1 cut(s) 221
BseMII CTCAG 3 cut(s) 78, 171, 294
BseNI ACTGG 1 cut(s) 157
BsgI GTGCAG 1 cut(s) 50
BshFI GGCC 1 cut(s) 277
BsnI GGCC 1 cut(s) 277
Bsp143I GATC 4 cut(s) 112, 142, 160, 600
BspACI CCGC 1 cut(s) 478
BspANI GGCC 1 cut(s) 277
BspCNI CTCAG 3 cut(s) 79, 172, 293
BspPI GGATC 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 221
BsrI ACTGG 1 cut(s) 157
BssMI GATC 4 cut(s) 112, 142, 160, 600
Bst2UI CCWGG 1 cut(s) 261
Bst4CI ACNGT 1 cut(s) 235
BstAPI GCANNNNNTGC 1 cut(s) 223
BstC8I GCNNGC 2 cut(s) 219, 339
BstDEI CTNAG 3 cut(s) 87, 180, 280
BstF5I GGATG 2 cut(s) 190, 482
BstKTI GATC 4 cut(s) 115, 145, 163, 603
BstMBI GATC 4 cut(s) 112, 142, 160, 600
BstMWI GCNNNNNNNGC 2 cut(s) 56, 223
BstNI CCWGG 1 cut(s) 261
BstNSI RCATGY 2 cut(s) 14, 196
BstSCI CCNGG 1 cut(s) 259
BstSFI CTRYAG 1 cut(s) 351
Bsu36I CCTNAGG 1 cut(s) 280
BsuI GTATCC 1 cut(s) 556
BsuRI GGCC 1 cut(s) 277
BtsCI GGATG 2 cut(s) 190, 482
Cac8I GCNNGC 2 cut(s) 219, 339
Cfr13I GGNCC 1 cut(s) 276
CviAII CATG 4 cut(s) 11, 193, 256, 528
CviJI RGCY 4 cut(s) 121, 277, 341, 502
CviKI_1 RGCY 4 cut(s) 121, 277, 341, 502
DdeI CTNAG 3 cut(s) 87, 180, 280
DpnI GATC 4 cut(s) 114, 144, 162, 602
DpnII GATC 4 cut(s) 112, 142, 160, 600
Eco57I CTGAAG 1 cut(s) 137
Eco81I CCTNAGG 1 cut(s) 280
EcoRII CCWGG 1 cut(s) 259
EcoT22I ATGCAT 1 cut(s) 529
FaeI CATG 4 cut(s) 14, 196, 259, 531
FalI AAGNNNNNCTT 2 cut(s) 34, 66
FatI CATG 4 cut(s) 10, 192, 255, 527
FbaI TGATCA 2 cut(s) 142, 600
FblI GTMKAC 1 cut(s) 149
FokI GGATG 2 cut(s) 197, 469
FspBI CTAG 2 cut(s) 293, 419
HaeIII GGCC 1 cut(s) 277
Hin1II CATG 4 cut(s) 14, 196, 259, 531
HincII GTYRAC 1 cut(s) 150
HindII GTYRAC 1 cut(s) 150
HinfI GANTC 2 cut(s) 134, 463
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 2 cut(s) 150, 190
Hpy188I TCNGA 3 cut(s) 37, 117, 468
Hpy188III TCNNGA 1 cut(s) 301
Hpy8I GTNNAC 2 cut(s) 150, 190
HpyAV CCTTC 1 cut(s) 260
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4V TGCA 7 cut(s) 31, 59, 101, 217, 226, 337, 527
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 223
HpyF3I CTNAG 3 cut(s) 87, 180, 280
Hsp92II CATG 4 cut(s) 14, 196, 259, 531
Ksp22I TGATCA 2 cut(s) 142, 600
Kzo9I GATC 4 cut(s) 112, 142, 160, 600
LpnPI CCDG 5 cut(s) 138, 246, 267, 273, 339
LweI GCATC 1 cut(s) 294
MaeI CTAG 2 cut(s) 293, 419
MaeIII GTNAC 2 cut(s) 94, 395
MalI GATC 4 cut(s) 114, 144, 162, 602
MboI GATC 4 cut(s) 112, 142, 160, 600
MluCI AATT 3 cut(s) 320, 565, 593
MnlI CCTC 7 cut(s) 82, 151, 175, 180, 289, 392, 423
Mph1103I ATGCAT 1 cut(s) 529
MroXI GAANNNNTTC 1 cut(s) 473
MseI TTAA 2 cut(s) 414, 522
MslI CAYNNNNRTG 3 cut(s) 15, 487, 514
MspR9I CCNGG 1 cut(s) 261
MvaI CCWGG 1 cut(s) 261
MwoI GCNNNNNNNGC 2 cut(s) 56, 223
NdeII GATC 4 cut(s) 112, 142, 160, 600
NlaIII CATG 4 cut(s) 14, 196, 259, 531
NmuCI GTSAC 1 cut(s) 395
NsiI ATGCAT 1 cut(s) 529
NspI RCATGY 2 cut(s) 14, 196
PciI ACATGT 1 cut(s) 10
PcsI WCGNNNNNNNCGW 1 cut(s) 59
PdmI GAANNNNTTC 1 cut(s) 473
PfeI GAWTC 2 cut(s) 134, 463
PscI ACATGT 1 cut(s) 10
PshBI ATTAAT 1 cut(s) 522
Psp6I CCWGG 1 cut(s) 259
PspGI CCWGG 1 cut(s) 259
PspPI GGNCC 1 cut(s) 276
RseI CAYNNNNRTG 3 cut(s) 15, 487, 514
SalI GTCGAC 1 cut(s) 148
SaqAI TTAA 2 cut(s) 414, 522
Sau3AI GATC 4 cut(s) 112, 142, 160, 600
Sau96I GGNCC 1 cut(s) 276
ScrFI CCNGG 1 cut(s) 261
SetI ASST 6 cut(s) 112, 123, 252, 343, 420, 514
SfaNI GCATC 1 cut(s) 294
SfcI CTRYAG 1 cut(s) 351
SmiMI CAYNNNNRTG 3 cut(s) 15, 487, 514
Sse9I AATT 3 cut(s) 320, 565, 593
SsiI CCGC 1 cut(s) 478
SspI AATATT 1 cut(s) 558
SspMI CTAG 2 cut(s) 293, 419
StyD4I CCNGG 1 cut(s) 259
TaaI ACNGT 1 cut(s) 235
TaqI TCGA 1 cut(s) 149
TasI AATT 3 cut(s) 320, 565, 593
TfiI GAWTC 2 cut(s) 134, 463
Tru1I TTAA 2 cut(s) 414, 522
Tru9I TTAA 2 cut(s) 414, 522
TseFI GTSAC 1 cut(s) 395
Tsp45I GTSAC 1 cut(s) 395
TspDTI ATGAA 2 cut(s) 364, 559
VspI ATTAAT 1 cut(s) 522
XapI RAATTY 1 cut(s) 565
XceI RCATGY 2 cut(s) 14, 196
XmiI GTMKAC 1 cut(s) 149
XmnI GAANNNNTTC 1 cut(s) 473
XspI CTAG 2 cut(s) 293, 419
Zsp2I ATGCAT 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.