Rh6AG005300

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000686
Physical Location & Seq
Reverse (-)
729304 .. 732726
3423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG005300.1

Sequence Viewer

Length: 1221 bp
ATGTTCAAGAAGGGAGTGGAAGCTCGGTCGCATCAGAGACTGTCAGGGGCTGACAGGAAGAAGCTGAAGAGGACCTCCCAAGGGGGCGAGGTTTCTCGATATGTTATTGGAGGGGCAGATTTGATGTTTCCCGGTTTCAGCATACCTGCTGAAGGTTTACCATCGTTTATAGCTGGGGAAGCATGGGCGGTTAAAGTTCCAGGAAATCCAGCTCCCATTGCTGTGGGAACTACTACAATGAGTTGCACTGAAGCATTAAAAGCTGGATTGCGTGGAAAAGCCTTAAAAATTGCTCACTATTATGGTGACTTACTTTGGGAATCAGTTGAGGGCCCTTATGTGCCAAATGCAGGTTTCTTAGAAGATGCTGTTTTTGAGGATCCTACCTTGTCATCAGCTCAAATTTCTGATCCATATAATGGTGCAAATGATGGATCACATGATCAACTGAATGGCATTGATGATAAGGAAATGGGGCAACATGTTGATGTTGCTGATGTGCAGTCTGAACCAAGTTCTGCTTCGGTTGCACCGATTGATGTTTGGAATGAGATTGCTGAGGAAGCAACTGCAAATGTAGGTGATCTGAAGCTAACAGATGATGATTCTGCTAATCAGTCGACTGGGGAGGATCAACATCCTCTTTCTGCTGAGGATGTGGACATGCTTTTGGAAAAATGCCTTTTGCAAGCATTGCATACAACTGTCAAAGGCAAAGACCTTCCCTTGCCCGGAAGCACTCTATGGTCAAACTATGTTTTGCCTTGTAGGCCTTCAGGCATCACGCTAGACATCAAGAAATCATCACACAACAAATTATCAAAGTGGTTACAAGCTAAATGTTCTACAGGATTGATTAGAGTGAAGGAAGATAAATATAAGGAGTCTGTATTACTTTCTATTGACCGCAGCCATCCAGATTACTCATCTTTCAAGCCAGAGAAACGACAAGTTGAAGAAGCTGTTCAAACTGGTGTTCCTGCTGTCAGTGAAAGTTGGTCGGTCAAAATCCTTGAAGTGGCTGAGATCTATAAACCAAGTGTACATGTAAATCCAGTATTTGCTTCTGTAGGTGCTGACACAGGCGAGCTTTACAGTGGTTCGGATGCCACTGACATTGCGTTTAAATATGTTGAAAAGGAAAACCTGGTTAAGCCGACAGATAAATCCATTGTGATTTTGGATGCGATATTGTGTGATGCACTGTTCAAAGGGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SUI1

Protein Analysis

406

Amino Acids

43.8

Weight (kDa)

4.97

Isoelectric Point (pI)

33.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUA_elF2D PF26292 30 - 103 3.3e-21 elF2D, PUA domain
WHD_eIF2D PF25304 221 - 301 3.1e-27 eIF2D winged helix domain
SWIB_eIF2D PF26291 344 - 403 6.5e-11 eIF2D, SWIB domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000686)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71350
fragaria_vesca FvH4_2g00430 FvH4_2g00430 FvH4_2g00430 FvH4_2g00430
malus_domestica MD10G1002600.v1.1
prunus_persica Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0249251 RchiOBHm_Chr6g0249261 RchiOBHm_Chr6g0249701 RchiOBHm_Chr6g0249761 RchiOBHm_Chr6g0249851 RchiOBHm_Chr6g0249861 RchiOBHm_Chr6g0249871 RchiOBHm_Chr6g0249891 RchiOBHm_Chr6g0249901 RchiOBHm_Chr6g0249911 RchiOBHm_Chr6g0249981 RchiOBHm_Chr6g0249991 RchiOBHm_Chr6g0250001 RchiOBHm_Chr6g0250151 RchiOBHm_Chr6g0250171 RchiOBHm_Chr6g0250191 RchiOBHm_Chr6g0250211 RchiOBHm_Chr6g0250221 RchiOBHm_Chr6g0250321 RchiOBHm_Chr6g0250391
rosa_laevigata RLG00000015543 RLG00000015548
rosa_multiflora Rmu_sc0000411.1_g000004 Rmu_sc0000411.1_g000009 Rmu_sc0000704.1_g000007 Rmu_sc0000704.1_g000011 Rmu_sc0001209.1_g000038 Rmu_sc0001209.1_g000042 Rmu_sc0001209.1_g000048 Rmu_sc0003293.1_g000005 Rmu_sc0004691.1_g000005 Rmu_sc0004691.1_g000007 Rmu_sc0004691.1_g000009 Rmu_sc0006032.1_g000001 Rmu_sc0006032.1_g000004 Rmu_sc0006032.1_g000005 Rmu_sc0008209.1_g000003 Rmu_sc0008209.1_g000010 Rmu_sc0009002.1_g000001 Rmu_sc0009002.1_g000005 Rmu_sc0009002.1_g000009
rosa_roxburghii Rroxscaffold_3G00218110 Rroxscaffold_7G00217270 Rroxscaffold_7G00217290 Rroxscaffold_7G00217300 Rroxscaffold_7G00217340
rosa_rugosa Rorug05G0494700 Rorug05G0495000 Rorug05G0496000 Rorug05G0498100 Rorug05G0498200
rosa_samantha Rh6AG000200 Rh6AG000300 Rh6AG004700 Rh6AG004800 Rh6AG005200 Rh6AG005300 Rh6AG005600 Rh6BG005500 Rh6BG005600 Rh6CG003700 Rh6DG003600
rosa_wichuraiana Rw6G000560 Rw6G000600 Rw6G000620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 154, 341
AccB7I CCANNNNNTGG 1 cut(s) 419
AccI GTMKAC 1 cut(s) 620
AciI CCGC 2 cut(s) 188, 907
AclWI GGATC 5 cut(s) 374, 387, 404, 442, 639
AcsI RAATTY 1 cut(s) 402
AcuI CTGAAG 5 cut(s) 86, 171, 270, 608, 759
AfaI GTAC 1 cut(s) 1044
AfiI CCNNNNNNNGG 6 cut(s) 81, 152, 350, 419, 731, 1018
AflIII ACRYGT 2 cut(s) 481, 1045
AgsI TTSAA 7 cut(s) 7, 934, 956, 968, 1016, 1136, 1210
AjnI CCWGG 2 cut(s) 199, 1146
AjuI GAANNNNNNNTTGG 2 cut(s) 505, 537
Alw26I GTCTC 1 cut(s) 31
AlwI GGATC 5 cut(s) 374, 387, 404, 442, 639
AlwNI CAGNNNCTG 2 cut(s) 40, 50
AoxI GGCC 3 cut(s) 331, 770, 1214
ApaI GGGCCC 1 cut(s) 335
ApeKI GCWGC 1 cut(s) 909
ApoI RAATTY 1 cut(s) 402
Asp700I GAANNNNTTC 1 cut(s) 963
AspS9I GGNCC 4 cut(s) 72, 331, 332, 1214
AsuC2I CCSGG 2 cut(s) 132, 732
AsuHPI GGTGA 2 cut(s) 317, 593
AvaII GGWCC 1 cut(s) 72
BaeGI GKGCMC 1 cut(s) 335
BamHI GGATCC 1 cut(s) 379
BanII GRGCYC 1 cut(s) 335
BbvCI CCTCAGC 2 cut(s) 558, 651
BbvI GCAGC 1 cut(s) 921
BccI CCATC 3 cut(s) 169, 425, 921
BciT130I CCWGG 2 cut(s) 201, 1148
BclI TGATCA 1 cut(s) 442
BcnI CCSGG 2 cut(s) 132, 732
BcoDI GTCTC 1 cut(s) 31
BfaI CTAG 1 cut(s) 788
BfmI CTRYAG 2 cut(s) 846, 1068
BfuAI ACCTGC 2 cut(s) 154, 341
BglI GCCNNNNNGGC 1 cut(s) 769
BglII AGATCT 1 cut(s) 1026
BisI GCNGC 1 cut(s) 910
BlsI GCNGC 1 cut(s) 911
Bme1390I CCNGG 4 cut(s) 132, 201, 732, 1148
Bme18I GGWCC 1 cut(s) 72
BmgT120I GGNCC 4 cut(s) 72, 331, 332, 1214
BmiI GGNNCC 2 cut(s) 333, 381
BmrFI CCNGG 4 cut(s) 132, 201, 732, 1148
BmrI ACTGGG 1 cut(s) 633
BmsI GCATC 6 cut(s) 40, 355, 789, 1096, 1174, 1189
BmuI ACTGGG 1 cut(s) 633
Bpu10I CCTNAGC 2 cut(s) 558, 651
BpuMI CCSGG 2 cut(s) 132, 732
BsaBI GATNNNNATC 1 cut(s) 636
BsaJI CCNNGG 1 cut(s) 79
Bsc4I CCNNNNNNNGG 6 cut(s) 81, 152, 350, 419, 731, 1018
Bse1I ACTGG 3 cut(s) 628, 976, 1055
Bse3DI GCAATG 3 cut(s) 216, 692, 1116
Bse8I GATNNNNATC 1 cut(s) 636
BseBI CCWGG 2 cut(s) 201, 1148
BseDI CCNNGG 1 cut(s) 79
BseGI GGATG 5 cut(s) 637, 661, 913, 1111, 1189
BseJI GATNNNNATC 1 cut(s) 636
BseLI CCNNNNNNNGG 6 cut(s) 81, 152, 350, 419, 731, 1018
BseMI GCAATG 3 cut(s) 216, 692, 1116
BseMII CTCAG 3 cut(s) 549, 642, 1014
BseNI ACTGG 3 cut(s) 628, 976, 1055
BseSI GKGCMC 1 cut(s) 335
BseXI GCAGC 1 cut(s) 921
BseYI CCCAGC 1 cut(s) 173
BsgI GTGCAG 1 cut(s) 521
Bsh1285I CGRYCG 1 cut(s) 29
BshFI GGCC 3 cut(s) 333, 772, 1216
BsiEI CGRYCG 1 cut(s) 29
BsiSI CCGG 2 cut(s) 132, 732
BslI CCNNNNNNNGG 6 cut(s) 81, 152, 350, 419, 731, 1018
BsmAI GTCTC 1 cut(s) 31
BsnI GGCC 3 cut(s) 333, 772, 1216
Bsp120I GGGCCC 1 cut(s) 331
Bsp1286I GDGCHC 1 cut(s) 335
Bsp1407I TGTACA 1 cut(s) 1042
Bsp143I GATC 7 cut(s) 379, 409, 434, 442, 583, 631, 1026
BspACI CCGC 2 cut(s) 188, 907
BspANI GGCC 3 cut(s) 333, 772, 1216
BspCNI CTCAG 3 cut(s) 550, 643, 1015
BspLI GGNNCC 2 cut(s) 333, 381
BspMI ACCTGC 2 cut(s) 154, 341
BspPI GGATC 5 cut(s) 374, 387, 404, 442, 639
BsrDI GCAATG 3 cut(s) 216, 692, 1116
BsrGI TGTACA 1 cut(s) 1042
BsrI ACTGG 3 cut(s) 628, 976, 1055
BssECI CCNNGG 1 cut(s) 79
BssMI GATC 7 cut(s) 379, 409, 434, 442, 583, 631, 1026
BssT1I CCWWGG 1 cut(s) 79
Bst2UI CCWGG 2 cut(s) 201, 1148
Bst4CI ACNGT 4 cut(s) 42, 706, 1097, 1206
Bst6I CTCTTC 1 cut(s) 62
BstAPI GCANNNNNTGC 1 cut(s) 694
BstAUI TGTACA 1 cut(s) 1042
BstC8I GCNNGC 2 cut(s) 690, 1088
BstDEI CTNAG 4 cut(s) 358, 558, 651, 1023
BstENI CCTNNNNNAGG 1 cut(s) 150
BstF5I GGATG 5 cut(s) 637, 661, 913, 1111, 1189
BstKTI GATC 7 cut(s) 382, 412, 437, 445, 586, 634, 1029
BstMAI GTCTC 1 cut(s) 31
BstMBI GATC 7 cut(s) 379, 409, 434, 442, 583, 631, 1026
BstMCI CGRYCG 1 cut(s) 29
BstMWI GCNNNNNNNGC 7 cut(s) 179, 218, 260, 527, 563, 694, 769
BstNI CCWGG 2 cut(s) 201, 1148
BstNSI RCATGY 3 cut(s) 485, 667, 1049
BstSCI CCNGG 4 cut(s) 130, 199, 730, 1146
BstSFI CTRYAG 2 cut(s) 846, 1068
BstSLI GKGCMC 1 cut(s) 335
BstV1I GCAGC 1 cut(s) 921
BstX2I RGATCY 2 cut(s) 379, 1026
BstXI CCANNNNNNTGG 1 cut(s) 223
BstYI RGATCY 2 cut(s) 379, 1026
BsuRI GGCC 3 cut(s) 333, 772, 1216
BtsCI GGATG 5 cut(s) 637, 661, 913, 1111, 1189
BtsIMutI CAGTG 5 cut(s) 246, 994, 1102, 1110, 1202
BveI ACCTGC 2 cut(s) 154, 341
Cac8I GCNNGC 2 cut(s) 690, 1088
CaiI CAGNNNCTG 2 cut(s) 40, 50
Cfr13I GGNCC 4 cut(s) 72, 331, 332, 1214
CsiI ACCWGGT 1 cut(s) 1146
Csp6I GTAC 1 cut(s) 1043
CspCI CAANNNNNGTGG 2 cut(s) 1099, 1134
CviAII CATG 5 cut(s) 183, 440, 482, 664, 1046
CviQI GTAC 1 cut(s) 1043
DdeI CTNAG 4 cut(s) 358, 558, 651, 1023
DpnI GATC 7 cut(s) 381, 411, 436, 444, 585, 633, 1028
DpnII GATC 7 cut(s) 379, 409, 434, 442, 583, 631, 1026
DraI TTTAAA 1 cut(s) 1126
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Eco130I CCWWGG 1 cut(s) 79
Eco147I AGGCCT 1 cut(s) 772
Eco24I GRGCYC 1 cut(s) 335
Eco47I GGWCC 1 cut(s) 72
Eco57I CTGAAG 5 cut(s) 86, 171, 270, 608, 759
EcoNI CCTNNNNNAGG 1 cut(s) 150
EcoO109I RGGNCCY 3 cut(s) 72, 331, 332
EcoRII CCWGG 2 cut(s) 199, 1146
EcoT14I CCWWGG 1 cut(s) 79
EcoT38I GRGCYC 1 cut(s) 335
ErhI CCWWGG 1 cut(s) 79
FaeI CATG 5 cut(s) 186, 443, 485, 667, 1049
FalI AAGNNNNNCTT 2 cut(s) 505, 537
FatI CATG 5 cut(s) 182, 439, 481, 663, 1045
FbaI TGATCA 1 cut(s) 442
FblI GTMKAC 1 cut(s) 620
Fnu4HI GCNGC 1 cut(s) 910
FokI GGATG 5 cut(s) 624, 668, 900, 1118, 1196
FriOI GRGCYC 1 cut(s) 335
Fsp4HI GCNGC 1 cut(s) 910
FspBI CTAG 1 cut(s) 788
GluI GCNGC 1 cut(s) 910
GsaI CCCAGC 1 cut(s) 177
HaeIII GGCC 3 cut(s) 333, 772, 1216
HapII CCGG 2 cut(s) 132, 732
Hin1II CATG 5 cut(s) 186, 443, 485, 667, 1049
HincII GTYRAC 1 cut(s) 621
HindII GTYRAC 1 cut(s) 621
HinfI GANTC 3 cut(s) 320, 605, 884
HpaII CCGG 2 cut(s) 132, 732
HphI GGTGA 2 cut(s) 317, 593
Hpy166II GTNNAC 4 cut(s) 158, 621, 661, 1043
Hpy188I TCNGA 5 cut(s) 36, 409, 508, 588, 1105
Hpy188III TCNNGA 4 cut(s) 7, 96, 796, 917
Hpy8I GTNNAC 4 cut(s) 158, 621, 661, 1043
HpyAV CCTTC 5 cut(s) 4, 146, 731, 783, 859
HpyCH4III ACNGT 4 cut(s) 42, 706, 1097, 1206
HpyCH4V TGCA 9 cut(s) 246, 350, 425, 502, 530, 572, 688, 697, 1202
HpyF10VI GCNNNNNNNGC 7 cut(s) 179, 218, 260, 527, 563, 694, 769
HpyF3I CTNAG 4 cut(s) 358, 558, 651, 1023
Hsp92II CATG 5 cut(s) 186, 443, 485, 667, 1049
Ksp22I TGATCA 1 cut(s) 442
Kzo9I GATC 7 cut(s) 379, 409, 434, 442, 583, 631, 1026
LmnI GCTCC 1 cut(s) 217
Lsp1109I GCAGC 1 cut(s) 921
LweI GCATC 6 cut(s) 40, 355, 789, 1096, 1174, 1189
MabI ACCWGGT 1 cut(s) 1146
MaeI CTAG 1 cut(s) 788
MaeIII GTNAC 2 cut(s) 305, 828
MalI GATC 7 cut(s) 381, 411, 436, 444, 585, 633, 1028
MboI GATC 7 cut(s) 379, 409, 434, 442, 583, 631, 1026
MboII GAAGA 5 cut(s) 70, 79, 374, 881, 968
MflI RGATCY 2 cut(s) 379, 1026
MhlI GDGCHC 1 cut(s) 335
MluCI AATT 3 cut(s) 288, 402, 815
MlyI GAGTC 1 cut(s) 893
MroXI GAANNNNTTC 1 cut(s) 963
MseI TTAA 5 cut(s) 192, 257, 284, 1125, 1152
MslI CAYNNNNRTG 3 cut(s) 221, 300, 486
MspI CCGG 2 cut(s) 132, 732
MspR9I CCNGG 4 cut(s) 132, 201, 732, 1148
MvaI CCWGG 2 cut(s) 201, 1148
MwoI GCNNNNNNNGC 7 cut(s) 179, 218, 260, 527, 563, 694, 769
NciI CCSGG 2 cut(s) 132, 732
NdeII GATC 7 cut(s) 379, 409, 434, 442, 583, 631, 1026
NlaIII CATG 5 cut(s) 186, 443, 485, 667, 1049
NlaIV GGNNCC 2 cut(s) 333, 381
NmuCI GTSAC 1 cut(s) 305
NspI RCATGY 3 cut(s) 485, 667, 1049
PceI AGGCCT 1 cut(s) 772
PciI ACATGT 2 cut(s) 481, 1045
PcsI WCGNNNNNNNCGW 1 cut(s) 530
PdmI GAANNNNTTC 1 cut(s) 963
PfeI GAWTC 2 cut(s) 320, 605
PflMI CCANNNNNTGG 1 cut(s) 419
PfoI TCCNGGA 1 cut(s) 199
PkrI GCNGC 1 cut(s) 911
PleI GAGTC 1 cut(s) 892
PpsI GAGTC 1 cut(s) 892
PpuMI RGGWCCY 1 cut(s) 72
PscI ACATGT 2 cut(s) 481, 1045
Psp5II RGGWCCY 1 cut(s) 72
Psp6I CCWGG 2 cut(s) 199, 1146
PspFI CCCAGC 1 cut(s) 173
PspGI CCWGG 2 cut(s) 199, 1146
PspN4I GGNNCC 2 cut(s) 333, 381
PspOMI GGGCCC 1 cut(s) 331
PspPI GGNCC 4 cut(s) 72, 331, 332, 1214
PspPPI RGGWCCY 1 cut(s) 72
PstNI CAGNNNCTG 2 cut(s) 40, 50
PsuI RGATCY 2 cut(s) 379, 1026
RsaI GTAC 1 cut(s) 1044
RsaNI GTAC 1 cut(s) 1043
RseI CAYNNNNRTG 3 cut(s) 221, 300, 486
SalI GTCGAC 1 cut(s) 619
SaqAI TTAA 5 cut(s) 192, 257, 284, 1125, 1152
SatI GCNGC 1 cut(s) 910
Sau3AI GATC 7 cut(s) 379, 409, 434, 442, 583, 631, 1026
Sau96I GGNCC 4 cut(s) 72, 331, 332, 1214
SchI GAGTC 1 cut(s) 893
ScrFI CCNGG 4 cut(s) 132, 201, 732, 1148
SduI GDGCHC 1 cut(s) 335
SexAI ACCWGGT 1 cut(s) 1146
SfaNI GCATC 6 cut(s) 40, 355, 789, 1096, 1174, 1189
SfcI CTRYAG 2 cut(s) 846, 1068
SinI GGWCC 1 cut(s) 72
SmiMI CAYNNNNRTG 3 cut(s) 221, 300, 486
Sse9I AATT 3 cut(s) 288, 402, 815
SseBI AGGCCT 1 cut(s) 772
SsiI CCGC 2 cut(s) 188, 907
SspMI CTAG 1 cut(s) 788
StuI AGGCCT 1 cut(s) 772
StyD4I CCNGG 4 cut(s) 130, 199, 730, 1146
StyI CCWWGG 1 cut(s) 79
TaaI ACNGT 4 cut(s) 42, 706, 1097, 1206
TaqI TCGA 2 cut(s) 97, 620
TaqII GACCGA 2 cut(s) 15, 991
TasI AATT 3 cut(s) 288, 402, 815
TatI WGTACW 1 cut(s) 1042
TfiI GAWTC 2 cut(s) 320, 605
Tru1I TTAA 5 cut(s) 192, 257, 284, 1125, 1152
Tru9I TTAA 5 cut(s) 192, 257, 284, 1125, 1152
TscAI CASTG 5 cut(s) 253, 994, 1102, 1117, 1209
TseFI GTSAC 1 cut(s) 305
TseI GCWGC 1 cut(s) 909
Tsp45I GTSAC 1 cut(s) 305
TspRI CASTG 5 cut(s) 253, 994, 1102, 1117, 1209
Van91I CCANNNNNTGG 1 cut(s) 419
VpaK11BI GGWCC 1 cut(s) 72
XagI CCTNNNNNAGG 1 cut(s) 150
XapI RAATTY 1 cut(s) 402
XceI RCATGY 3 cut(s) 485, 667, 1049
XcmI CCANNNNNNNNNTGG 1 cut(s) 1177
XmiI GTMKAC 1 cut(s) 620
XmnI GAANNNNTTC 1 cut(s) 963
XspI CTAG 1 cut(s) 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.