MD12G1083900.v1.1

Glucan endo-1,3-beta-glucosidase, basic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000485
Physical Location & Seq
Reverse (-)
10225478 .. 10227107
1630 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1083900.v1.1.491

Sequence Viewer

Length: 999 bp
ATGGTTTCCATAATGCTACTTGTTGGAGCAAGCATTGGTGTTTGTTATGGAACGCTTGGAAACTTGCCACCCTCAGGAGAAGTAATAGCTCTCTACAAACAATATAACATCCAAAAACTACGGCTATATGCTCCCAACGACGACGCTCTCCGAGCCCTTCAAGGCTCCACCATCGAGGTCATGCTTGGCATCGAAAATGAGCGTCTTCAAGACATTTCTTCTAACCAAGCCCGTGCAGATGATTGGGTTCAAAGAAATGTCAAAAGCTATCCGAATGTCAATTTCAAATACATCGCCGTTGGAAACGAAGTAGATGCCCTGGGTAACCGAGCACCCTATGTTGGCCCTGCGATGGACAATATTCAAAATGCAATTTCTAGGGCTGGTCTTGCAAACCAAATCAAAGTCTCCACTGCCGTTTTTCCTATAATTCTCAAGGAATCATTTCCTCCATCAAAAGGCTTATTCCGGCCGGATTATAGACCATTTCTCGATCCTATCATAAGCTTCCTAGTCAGAAACCAATCTCCTTTACTTGTTAACATGTACACATATTTTAGTTACATTCAAAATAGTCAGAAGATTAAACTCGAGTACGCTCTTTTTACATCTCCATCGGTTGTAGTCAATGACGATCGGCTTGGGTATCAAAACCTGTTTGATGCAATTCTAGATGCACATTACTCAGTATTAGAGAAGGCTGGAGGCGGCTCTCTGAAAATTGTTGTATCGGAGACTGGTTGGCCCTCTGCTGGCGGAGATGAGCAAGTGACAACGACCGAGAATGCAAGAATTTACAACTCAAATTTGATTAAACATGTGAAGGGTGGGACTCCGAAGAAACCTGAAAGCCCCATAGACACTTACGTGTTTGCCGTGTTTGCCATGTTTGATGAGAACCAAAAACCTGGAGAAGAGACCGAGAGACACTGGGGCAGTGGCGGATCCAGGAATCTTACCCCAAGGGGTCGCAGTGTAAAAGTTCAAATAAAAATTTAG

Protein Analysis

333

Amino Acids

36.71

Weight (kDa)

8.41

Isoelectric Point (pI)

37.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 12 - 312 7.8e-98 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000485)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16260 AT4G16260
fragaria_vesca FvH4_4g19500 FvH4_5g06210 FvH4_6g24640 FvH4_6g24680 FvH4_6g37200 FvH4_6g37201 FvH4_6g37370
malus_domestica MD12G1083900.v1.1 MD12G1084100.v1.1
prunus_persica Prupe.1G121800_v2.0.a1 Prupe.7G051600_v2.0.a1 Prupe.7G052100_v2.0.a1
pyrus_communis pycom12g07050 pycom12g07100
rosa_chinensis RchiOBHm_Chr2g0149871 RchiOBHm_Chr2g0149881 RchiOBHm_Chr3g0484031 RchiOBHm_Chr3g0484041 RchiOBHm_Chr3g0484071 RchiOBHm_Chr3g0484141 RchiOBHm_Chr3g0484181 RchiOBHm_Chr3g0484211 RchiOBHm_Chr3g0484221 RchiOBHm_Chr3g0484251 RchiOBHm_Chr4g0424631 RchiOBHm_Chr7g0178181
rosa_laevigata RLG00000007444 RLG00000007447 RLG00000020451 RLG00000020452 RLG00000023238 RLG00000023240 RLG00000023241 RLG00000023243
rosa_multiflora Rmu_co8177358.1_g000001 Rmu_co8303623.1_g000001 Rmu_co8339607.1_g000001 Rmu_sc0000730.1_g000027 Rmu_sc0001899.1_g000006 Rmu_sc0001899.1_g000012 Rmu_sc0003477.1_g000003 Rmu_sc0003477.1_g000011 Rmu_sc0006371.1_g000005 Rmu_sc0007329.1_g000005 Rmu_sc0007855.1_g000006 Rmu_sc0017706.1_g000001 Rmu_ssc0000308.1_g000021 Rmu_ssc0000308.1_g000028
rosa_roxburghii Rroxscaffold_2G00097590 Rroxscaffold_5G00366550 Rroxscaffold_6G00397840 Rroxscaffold_6G00397890
rosa_rugosa Rorug02G0417000 Rorug02G0417000 Rorug03G0210200 Rorug03G0210400 Rorug04G0198700 Rorug06G0413900
rosa_samantha Rh2AG476400 Rh2BG487900 Rh2BG488000 Rh2CG461800 Rh2DG496700 Rh2DG497100 Rh3AG259000 Rh3AG259200 Rh3BG295300 Rh3BG295500 Rh3BG295600 Rh3BG295900 Rh3CG292800 Rh3CG292900 Rh3CG293300 Rh3DG288600 Rh3DG289100 Rh3DG289300 Rh4AG255300 Rh4BG260800 Rh4CG272000 Rh4DG255500 Rh7AG013600
rosa_wichuraiana Rw2G038830 Rw3G023320 Rw3G023360 Rw3G023370 Rw3G023380 Rw4G022070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 708, 756, 942
AclWI GGATC 3 cut(s) 488, 939, 952
AcoI YGGCCR 1 cut(s) 470
AcsI RAATTY 3 cut(s) 792, 805, 993
AfaI GTAC 2 cut(s) 548, 596
AfiI CCNNNNNNNGG 5 cut(s) 74, 341, 352, 458, 752
AflIII ACRYGT 3 cut(s) 543, 817, 867
AgsI TTSAA 7 cut(s) 161, 209, 251, 286, 365, 569, 986
AjnI CCWGG 3 cut(s) 318, 907, 947
AleI CACNNNNGTG 1 cut(s) 866
AluBI AGCT 3 cut(s) 89, 267, 507
AluI AGCT 3 cut(s) 89, 267, 507
Alw21I GWGCWC 1 cut(s) 334
Alw26I GTCTC 4 cut(s) 412, 728, 911, 919
AlwI GGATC 3 cut(s) 488, 939, 952
Ama87I CYCGRG 1 cut(s) 590
AoxI GGCC 3 cut(s) 343, 470, 743
ApoI RAATTY 3 cut(s) 792, 805, 993
Asp700I GAANNNNTTC 1 cut(s) 444
AspS9I GGNCC 2 cut(s) 344, 744
AvaI CYCGRG 1 cut(s) 590
AxyI CCTNAGG 1 cut(s) 73
BamHI GGATCC 1 cut(s) 944
BanII GRGCYC 1 cut(s) 157
BbsI GAAGAC 1 cut(s) 197
Bbv12I GWGCWC 1 cut(s) 334
BccI CCATC 4 cut(s) 179, 346, 460, 622
BceAI ACGGC 4 cut(s) 137, 281, 401, 860
BcgI CGANNNNNNTGC 2 cut(s) 296, 330
BciT130I CCWGG 3 cut(s) 320, 909, 949
BcoDI GTCTC 4 cut(s) 412, 728, 911, 919
BfaI CTAG 3 cut(s) 378, 512, 671
BisI GCNGC 1 cut(s) 709
BlsI GCNGC 1 cut(s) 710
Bme1390I CCNGG 3 cut(s) 320, 909, 949
BmeT110I CYCGRG 1 cut(s) 590
BmgT120I GGNCC 2 cut(s) 344, 744
BmiI GGNNCC 2 cut(s) 166, 946
BmrFI CCNGG 3 cut(s) 320, 909, 949
BmrI ACTGGG 1 cut(s) 940
BmsI GCATC 4 cut(s) 198, 304, 652, 664
BmuI ACTGGG 1 cut(s) 940
BpiI GAAGAC 1 cut(s) 197
BpmI CTGGAG 2 cut(s) 723, 930
BpuEI CTTGAG 1 cut(s) 419
BsaAI YACGTR 1 cut(s) 868
BsaI GGTCTC 1 cut(s) 911
BsaJI CCNNGG 3 cut(s) 318, 319, 962
Bsc4I CCNNNNNNNGG 5 cut(s) 74, 341, 352, 458, 752
Bse1I ACTGG 2 cut(s) 742, 935
Bse21I CCTNAGG 1 cut(s) 73
BseBI CCWGG 3 cut(s) 320, 909, 949
BseDI CCNNGG 3 cut(s) 318, 319, 962
BseGI GGATG 1 cut(s) 108
BseLI CCNNNNNNNGG 5 cut(s) 74, 341, 352, 458, 752
BseMII CTCAG 2 cut(s) 87, 699
BseNI ACTGG 2 cut(s) 742, 935
BseX3I CGGCCG 1 cut(s) 470
BsgI GTGCAG 1 cut(s) 255
Bsh1285I CGRYCG 3 cut(s) 473, 637, 780
BshFI GGCC 3 cut(s) 345, 472, 745
BsiEI CGRYCG 3 cut(s) 473, 637, 780
BsiHKAI GWGCWC 1 cut(s) 334
BsiHKCI CYCGRG 1 cut(s) 590
BsiSI CCGG 2 cut(s) 469, 473
BslFI GGGAC 1 cut(s) 844
BslI CCNNNNNNNGG 5 cut(s) 74, 341, 352, 458, 752
BsmAI GTCTC 4 cut(s) 412, 728, 911, 919
BsmFI GGGAC 1 cut(s) 844
BsmI GAATGC 1 cut(s) 790
BsnI GGCC 3 cut(s) 345, 472, 745
Bso31I GGTCTC 1 cut(s) 911
BsoBI CYCGRG 1 cut(s) 590
Bsp1286I GDGCHC 2 cut(s) 157, 334
Bsp1407I TGTACA 1 cut(s) 546
Bsp143I GATC 3 cut(s) 493, 634, 944
BspACI CCGC 3 cut(s) 708, 756, 942
BspANI GGCC 3 cut(s) 345, 472, 745
BspCNI CTCAG 2 cut(s) 86, 698
BspLI GGNNCC 2 cut(s) 166, 946
BspPI GGATC 3 cut(s) 488, 939, 952
BspTNI GGTCTC 1 cut(s) 911
BsrGI TGTACA 1 cut(s) 546
BsrI ACTGG 2 cut(s) 742, 935
BssECI CCNNGG 3 cut(s) 318, 319, 962
BssMI GATC 3 cut(s) 493, 634, 944
BssT1I CCWWGG 1 cut(s) 962
Bst2UI CCWGG 3 cut(s) 320, 909, 949
Bst6I CTCTTC 1 cut(s) 909
BstAUI TGTACA 1 cut(s) 546
BstBAI YACGTR 1 cut(s) 868
BstC8I GCNNGC 2 cut(s) 31, 754
BstDEI CTNAG 2 cut(s) 73, 685
BstEII GGTNACC 1 cut(s) 323
BstF5I GGATG 1 cut(s) 108
BstKTI GATC 3 cut(s) 496, 637, 947
BstMAI GTCTC 4 cut(s) 412, 728, 911, 919
BstMBI GATC 3 cut(s) 493, 634, 944
BstMCI CGRYCG 3 cut(s) 473, 637, 780
BstMWI GCNNNNNNNGC 3 cut(s) 152, 389, 881
BstNI CCWGG 3 cut(s) 320, 909, 949
BstNSI RCATGY 2 cut(s) 547, 821
BstPI GGTNACC 1 cut(s) 323
BstSCI CCNGG 3 cut(s) 318, 907, 947
BstV2I GAAGAC 1 cut(s) 197
BstX2I RGATCY 1 cut(s) 944
BstXI CCANNNNNNTGG 1 cut(s) 908
BstYI RGATCY 1 cut(s) 944
BstZI CGGCCG 1 cut(s) 470
Bsu36I CCTNAGG 1 cut(s) 73
BsuRI GGCC 3 cut(s) 345, 472, 745
BtgZI GCGATG 2 cut(s) 277, 365
BtsCI GGATG 1 cut(s) 108
BtsI GCAGTG 3 cut(s) 411, 943, 979
BtsIMutI CAGTG 4 cut(s) 411, 928, 943, 979
Cac8I GCNNGC 2 cut(s) 31, 754
Cfr13I GGNCC 2 cut(s) 344, 744
CseI GACGC 2 cut(s) 152, 191
Csp6I GTAC 2 cut(s) 547, 595
CviAII CATG 4 cut(s) 181, 544, 818, 886
CviQI GTAC 2 cut(s) 547, 595
DdeI CTNAG 2 cut(s) 73, 685
DpnI GATC 3 cut(s) 495, 636, 946
DpnII GATC 3 cut(s) 493, 634, 944
EaeI YGGCCR 1 cut(s) 470
EagI CGGCCG 1 cut(s) 470
Eam1104I CTCTTC 1 cut(s) 909
EarI CTCTTC 1 cut(s) 909
EciI GGCGGA 2 cut(s) 771, 957
EclXI CGGCCG 1 cut(s) 470
Eco130I CCWWGG 1 cut(s) 962
Eco24I GRGCYC 1 cut(s) 157
Eco31I GGTCTC 1 cut(s) 911
Eco52I CGGCCG 1 cut(s) 470
Eco81I CCTNAGG 1 cut(s) 73
Eco88I CYCGRG 1 cut(s) 590
Eco91I GGTNACC 1 cut(s) 323
EcoO65I GGTNACC 1 cut(s) 323
EcoRII CCWGG 3 cut(s) 318, 907, 947
EcoT14I CCWWGG 1 cut(s) 962
EcoT38I GRGCYC 1 cut(s) 157
ErhI CCWWGG 1 cut(s) 962
FaeI CATG 4 cut(s) 184, 547, 821, 889
FaqI GGGAC 1 cut(s) 844
FatI CATG 4 cut(s) 180, 543, 817, 885
Fnu4HI GCNGC 1 cut(s) 709
FokI GGATG 1 cut(s) 95
FriOI GRGCYC 1 cut(s) 157
Fsp4HI GCNGC 1 cut(s) 709
FspBI CTAG 3 cut(s) 378, 512, 671
GluI GCNGC 1 cut(s) 709
GsuI CTGGAG 2 cut(s) 723, 930
HaeIII GGCC 3 cut(s) 345, 472, 745
HapII CCGG 2 cut(s) 469, 473
HgaI GACGC 2 cut(s) 152, 191
Hin1II CATG 4 cut(s) 184, 547, 821, 889
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HindIII AAGCTT 1 cut(s) 505
HinfI GANTC 3 cut(s) 440, 832, 952
HpaI GTTAAC 1 cut(s) 541
HpaII CCGG 2 cut(s) 469, 473
Hpy166II GTNNAC 2 cut(s) 541, 549
Hpy188I TCNGA 7 cut(s) 152, 273, 518, 579, 717, 733, 837
Hpy188III TCNNGA 4 cut(s) 75, 209, 491, 671
Hpy8I GTNNAC 2 cut(s) 541, 549
Hpy99I CGWCG 2 cut(s) 143, 146
HpyAV CCTTC 3 cut(s) 167, 691, 817
HpyCH4IV ACGT 1 cut(s) 867
HpyCH4V TGCA 6 cut(s) 236, 371, 392, 665, 677, 788
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 389, 881
HpyF3I CTNAG 2 cut(s) 73, 685
HpySE526I ACGT 1 cut(s) 867
Hsp92II CATG 4 cut(s) 184, 547, 821, 889
KspAI GTTAAC 1 cut(s) 541
Kzo9I GATC 3 cut(s) 493, 634, 944
LmnI GCTCC 3 cut(s) 26, 136, 170
LweI GCATC 4 cut(s) 198, 304, 652, 664
MaeI CTAG 3 cut(s) 378, 512, 671
MaeII ACGT 1 cut(s) 867
MaeIII GTNAC 3 cut(s) 323, 560, 769
MalI GATC 3 cut(s) 495, 636, 946
MboI GATC 3 cut(s) 493, 634, 944
MboII GAAGA 5 cut(s) 197, 210, 592, 850, 926
MflI RGATCY 1 cut(s) 944
MhlI GDGCHC 2 cut(s) 157, 334
MluCI AATT 8 cut(s) 280, 372, 429, 666, 720, 792, 805, 993
MlyI GAGTC 1 cut(s) 826
MmeI TCCRAC 2 cut(s) 4, 280
MnlI CCTC 5 cut(s) 82, 169, 459, 698, 757
MroXI GAANNNNTTC 1 cut(s) 444
MseI TTAA 3 cut(s) 540, 585, 813
MslI CAYNNNNRTG 1 cut(s) 866
MspI CCGG 2 cut(s) 469, 473
MspR9I CCNGG 3 cut(s) 320, 909, 949
Mva1269I GAATGC 1 cut(s) 790
MvaI CCWGG 3 cut(s) 320, 909, 949
MwoI GCNNNNNNNGC 3 cut(s) 152, 389, 881
NdeII GATC 3 cut(s) 493, 634, 944
NlaIII CATG 4 cut(s) 184, 547, 821, 889
NlaIV GGNNCC 2 cut(s) 166, 946
NmuCI GTSAC 1 cut(s) 769
NspI RCATGY 2 cut(s) 547, 821
OliI CACNNNNGTG 1 cut(s) 866
PaeR7I CTCGAG 1 cut(s) 590
PasI CCCWGGG 1 cut(s) 319
PciI ACATGT 2 cut(s) 543, 817
PcsI WCGNNNNNNNCGW 1 cut(s) 873
PctI GAATGC 1 cut(s) 790
PdmI GAANNNNTTC 1 cut(s) 444
PfeI GAWTC 2 cut(s) 440, 952
PfoI TCCNGGA 1 cut(s) 947
PkrI GCNGC 1 cut(s) 710
Ple19I CGATCG 1 cut(s) 637
PleI GAGTC 1 cut(s) 826
PpsI GAGTC 1 cut(s) 826
Ppu21I YACGTR 1 cut(s) 868
PscI ACATGT 2 cut(s) 543, 817
Psp6I CCWGG 3 cut(s) 318, 907, 947
PspEI GGTNACC 1 cut(s) 323
PspGI CCWGG 3 cut(s) 318, 907, 947
PspN4I GGNNCC 2 cut(s) 166, 946
PspPI GGNCC 2 cut(s) 344, 744
PspXI VCTCGAGB 1 cut(s) 590
PsuI RGATCY 1 cut(s) 944
PvuI CGATCG 1 cut(s) 637
RsaI GTAC 2 cut(s) 548, 596
RsaNI GTAC 2 cut(s) 547, 595
RseI CAYNNNNRTG 1 cut(s) 866
SaqAI TTAA 3 cut(s) 540, 585, 813
SatI GCNGC 1 cut(s) 709
Sau3AI GATC 3 cut(s) 493, 634, 944
Sau96I GGNCC 2 cut(s) 344, 744
SchI GAGTC 1 cut(s) 826
ScrFI CCNGG 3 cut(s) 320, 909, 949
SduI GDGCHC 2 cut(s) 157, 334
SetI ASST 8 cut(s) 91, 180, 269, 509, 657, 847, 870, 910
SfaNI GCATC 4 cut(s) 198, 304, 652, 664
Sfr274I CTCGAG 1 cut(s) 590
SlaI CTCGAG 1 cut(s) 590
SmiMI CAYNNNNRTG 1 cut(s) 866
SmlI CTYRAG 2 cut(s) 434, 590
SmoI CTYRAG 2 cut(s) 434, 590
Sse9I AATT 8 cut(s) 280, 372, 429, 666, 720, 792, 805, 993
SsiI CCGC 3 cut(s) 708, 756, 942
SspI AATATT 1 cut(s) 361
SspMI CTAG 3 cut(s) 378, 512, 671
StyD4I CCNGG 3 cut(s) 318, 907, 947
StyI CCWWGG 1 cut(s) 962
TaiI ACGT 1 cut(s) 870
TaqI TCGA 4 cut(s) 174, 192, 492, 591
TaqII GACCGA 2 cut(s) 794, 935
TasI AATT 8 cut(s) 280, 372, 429, 666, 720, 792, 805, 993
TatI WGTACW 1 cut(s) 546
TauI GCSGC 1 cut(s) 711
TfiI GAWTC 2 cut(s) 440, 952
Tru1I TTAA 3 cut(s) 540, 585, 813
Tru9I TTAA 3 cut(s) 540, 585, 813
TscAI CASTG 4 cut(s) 418, 935, 943, 979
TseFI GTSAC 1 cut(s) 769
Tsp45I GTSAC 1 cut(s) 769
TspRI CASTG 4 cut(s) 418, 935, 943, 979
XapI RAATTY 3 cut(s) 792, 805, 993
XbaI TCTAGA 1 cut(s) 670
XceI RCATGY 2 cut(s) 547, 821
XhoI CTCGAG 1 cut(s) 590
XmnI GAANNNNTTC 1 cut(s) 444
XspI CTAG 3 cut(s) 378, 512, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.