RchiOBHm_Chr3g0484181

Glucan endo-1,3-beta-glucosidase, basic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000485
Physical Location & Seq
Forward (+)
30525024 .. 30526392
1369 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44889

Sequence Viewer

Length: 1053 bp
ATGGGAAAGGCAAATATACTTGCCAAAAACTCTTCCATGCTTTCAAAAATGCTACTTTTTGGAATACTAGTAATGGCTCATATAGCAGCAACTACAGGTTCCAAGATTGGTGTTTGTTATGGAACCTTGGGAAACGACTTACCGCCAGCACAGGAAGTAATATCTATGTTCGACCAGTATAGAATTCAGAAAATCCGTCTCTATGATCCTAACCAAGGAGCTCTCCAAGCCCTTAGAGGCTCCAACATCGAGGTCATGCTTGGTGTTGAAAATGAACGCCTTCAAGACATTTCTTCTAGCCAAGACAAGGCTAATTCCTGGGTCCAAAGTAATGTCCTAAACTATGGCAATGTCAATTTCAAATACATTGTTGTAGGAAACGAAATAGATCCTACAGGTCCACAAGCATCGTTTGTTGCCCCTGCAATGGAAAACATTCAAAAAGCAATTACTTCGGAGAATCTTGCCAGTAAAATTAAAGTCTCCACTGCCATTTCCACTGCCGTCCTCAAAGAACCCTCTTACCCTCCATCAAACGGAGCATTCCGTATCGACCATTTACCATTTCTTAATCCCATCATCGCCTTCTTAGCACGCAACCAATCTCCACTGCTTGTTAATCTATACCCTTATTTTACTTACGATCAAAACCGTCAGATTAATATCGAGTATGCTCTTTTTACATCTCCATCACCAGTAGTGCATGATGGCCAGCTTGCGTATCAAAACCTGTTTGATGCTATTCTCGATACAGTTTACTCGGCACTAGAGAGAGCTGGCAGCGGTTCCTTGGGAATCGTTGTATCGGAGTCTGGTTGGCCCTCTTCCGGTGGGGACGGAGAAGTGACTACAATTGAGAATGCAAGAATTTACAACTCCAATTTGATCAATCATGTGAAGGCTAATGGGACTCCAAAGAAACCTGGAAGCACTATAGAGGCTTATGTGTTTGCCATGTTTAATGAGAATCAAAAGAAAGGTCCAGAGATTGAGAGACATTGGGGGCTCTTCTACCCAACCAAACAGCCTAAATATCACATTGACTTCAATTAA

Protein Analysis

350

Amino Acids

38.49

Weight (kDa)

6.32

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 36 - 349 2.3e-108 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000485)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16260 AT4G16260
fragaria_vesca FvH4_4g19500 FvH4_5g06210 FvH4_6g24640 FvH4_6g24680 FvH4_6g37200 FvH4_6g37201 FvH4_6g37370
malus_domestica MD12G1083900.v1.1 MD12G1084100.v1.1
prunus_persica Prupe.1G121800_v2.0.a1 Prupe.7G051600_v2.0.a1 Prupe.7G052100_v2.0.a1
pyrus_communis pycom12g07050 pycom12g07100
rosa_chinensis RchiOBHm_Chr2g0149871 RchiOBHm_Chr2g0149881 RchiOBHm_Chr3g0484031 RchiOBHm_Chr3g0484041 RchiOBHm_Chr3g0484071 RchiOBHm_Chr3g0484141 RchiOBHm_Chr3g0484181 RchiOBHm_Chr3g0484211 RchiOBHm_Chr3g0484221 RchiOBHm_Chr3g0484251 RchiOBHm_Chr4g0424631 RchiOBHm_Chr7g0178181
rosa_laevigata RLG00000007444 RLG00000007447 RLG00000020451 RLG00000020452 RLG00000023238 RLG00000023240 RLG00000023241 RLG00000023243
rosa_multiflora Rmu_co8177358.1_g000001 Rmu_co8303623.1_g000001 Rmu_co8339607.1_g000001 Rmu_sc0000730.1_g000027 Rmu_sc0001899.1_g000006 Rmu_sc0001899.1_g000012 Rmu_sc0003477.1_g000003 Rmu_sc0003477.1_g000011 Rmu_sc0006371.1_g000005 Rmu_sc0007329.1_g000005 Rmu_sc0007855.1_g000006 Rmu_sc0017706.1_g000001 Rmu_ssc0000308.1_g000021 Rmu_ssc0000308.1_g000028
rosa_roxburghii Rroxscaffold_2G00097590 Rroxscaffold_5G00366550 Rroxscaffold_6G00397840 Rroxscaffold_6G00397890
rosa_rugosa Rorug02G0417000 Rorug02G0417000 Rorug03G0210200 Rorug03G0210400 Rorug04G0198700 Rorug06G0413900
rosa_samantha Rh2AG476400 Rh2BG487900 Rh2BG488000 Rh2CG461800 Rh2DG496700 Rh2DG497100 Rh3AG259000 Rh3AG259200 Rh3BG295300 Rh3BG295500 Rh3BG295600 Rh3BG295900 Rh3CG292800 Rh3CG292900 Rh3CG293300 Rh3DG288600 Rh3DG289100 Rh3DG289300 Rh4AG255300 Rh4BG260800 Rh4CG272000 Rh4DG255500 Rh7AG013600
rosa_wichuraiana Rw2G038830 Rw3G023320 Rw3G023360 Rw3G023370 Rw3G023380 Rw4G022070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 143, 783
AclWI GGATC 2 cut(s) 200, 383
AcoI YGGCCR 1 cut(s) 709
AcsI RAATTY 2 cut(s) 183, 867
AfiI CCNNNNNNNGG 5 cut(s) 215, 307, 427, 536, 827
AgsI TTSAA 6 cut(s) 45, 269, 284, 361, 440, 1048
AhlI ACTAGT 1 cut(s) 67
AjnI CCWGG 2 cut(s) 317, 922
AluBI AGCT 3 cut(s) 221, 715, 776
AluI AGCT 3 cut(s) 221, 715, 776
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 3 cut(s) 203, 487, 988
AlwI GGATC 2 cut(s) 200, 383
AoxI GGCC 2 cut(s) 709, 818
ApeKI GCWGC 2 cut(s) 86, 780
ApoI RAATTY 2 cut(s) 183, 867
ArsI GACNNNNNNTTYG 2 cut(s) 964, 996
AseI ATTAAT 1 cut(s) 660
Asp700I GAANNNNTTC 2 cut(s) 279, 435
AspS9I GGNCC 4 cut(s) 322, 398, 819, 980
AsuHPI GGTGA 1 cut(s) 684
AvaII GGWCC 3 cut(s) 322, 398, 980
BalI TGGCCA 1 cut(s) 711
BanII GRGCYC 2 cut(s) 223, 1008
Bbv12I GWGCWC 1 cut(s) 223
BbvI GCAGC 2 cut(s) 98, 792
BccI CCATC 4 cut(s) 538, 584, 697, 701
BceAI ACGGC 1 cut(s) 488
BcgI CGANNNNNNTGC 2 cut(s) 435, 469
BciT130I CCWGG 2 cut(s) 319, 924
BclI TGATCA 1 cut(s) 885
BcoDI GTCTC 3 cut(s) 203, 487, 988
BcuI ACTAGT 1 cut(s) 67
BfaI CTAG 3 cut(s) 68, 297, 767
BfmI CTRYAG 3 cut(s) 93, 393, 933
BisI GCNGC 2 cut(s) 87, 781
BlsI GCNGC 2 cut(s) 88, 782
Bme1390I CCNGG 2 cut(s) 319, 924
Bme18I GGWCC 3 cut(s) 322, 398, 980
BmgT120I GGNCC 4 cut(s) 322, 398, 819, 980
BmiI GGNNCC 5 cut(s) 100, 124, 241, 323, 787
BmrFI CCNGG 2 cut(s) 319, 924
BmsI GCATC 2 cut(s) 416, 727
BsaBI GATNNNNATC 1 cut(s) 662
BsaJI CCNNGG 4 cut(s) 126, 214, 318, 789
BsaWI WCCGGW 1 cut(s) 827
Bsc4I CCNNNNNNNGG 5 cut(s) 215, 307, 427, 536, 827
Bse1I ACTGG 3 cut(s) 175, 468, 695
Bse3DI GCAATG 2 cut(s) 355, 432
Bse8I GATNNNNATC 1 cut(s) 662
BseBI CCWGG 2 cut(s) 319, 924
BseDI CCNNGG 4 cut(s) 126, 214, 318, 789
BseJI GATNNNNATC 1 cut(s) 662
BseLI CCNNNNNNNGG 5 cut(s) 215, 307, 427, 536, 827
BseMI GCAATG 2 cut(s) 355, 432
BseNI ACTGG 3 cut(s) 175, 468, 695
BseXI GCAGC 2 cut(s) 98, 792
BshFI GGCC 2 cut(s) 711, 820
BsiHKAI GWGCWC 1 cut(s) 223
BsiSI CCGG 1 cut(s) 828
BslFI GGGAC 2 cut(s) 848, 922
BslI CCNNNNNNNGG 5 cut(s) 215, 307, 427, 536, 827
BsmAI GTCTC 3 cut(s) 203, 487, 988
BsmBI CGTCTC 1 cut(s) 203
BsmFI GGGAC 2 cut(s) 848, 922
BsmI GAATGC 2 cut(s) 542, 865
BsnI GGCC 2 cut(s) 711, 820
Bsp1286I GDGCHC 2 cut(s) 223, 1008
Bsp143I GATC 4 cut(s) 205, 388, 643, 885
BspACI CCGC 2 cut(s) 143, 783
BspANI GGCC 2 cut(s) 711, 820
BspLI GGNNCC 5 cut(s) 100, 124, 241, 323, 787
BspPI GGATC 2 cut(s) 200, 383
BspQI GCTCTTC 1 cut(s) 1013
BsrDI GCAATG 2 cut(s) 355, 432
BsrI ACTGG 3 cut(s) 175, 468, 695
BssECI CCNNGG 4 cut(s) 126, 214, 318, 789
BssMI GATC 4 cut(s) 205, 388, 643, 885
BssT1I CCWWGG 3 cut(s) 126, 214, 789
Bst2UI CCWGG 2 cut(s) 319, 924
Bst4CI ACNGT 2 cut(s) 653, 754
Bst6I CTCTTC 3 cut(s) 37, 829, 1013
BstC8I GCNNGC 5 cut(s) 147, 595, 713, 717, 778
BstDEI CTNAG 2 cut(s) 233, 589
BstENI CCTNNNNNAGG 1 cut(s) 213
BstKTI GATC 4 cut(s) 208, 391, 646, 888
BstMAI GTCTC 3 cut(s) 203, 487, 988
BstMBI GATC 4 cut(s) 205, 388, 643, 885
BstMWI GCNNNNNNNGC 3 cut(s) 83, 227, 590
BstNI CCWGG 2 cut(s) 319, 924
BstSCI CCNGG 2 cut(s) 317, 922
BstSFI CTRYAG 3 cut(s) 93, 393, 933
BstV1I GCAGC 2 cut(s) 98, 792
BstX2I RGATCY 1 cut(s) 388
BstYI RGATCY 1 cut(s) 388
BsuRI GGCC 2 cut(s) 711, 820
BtgZI GCGATG 1 cut(s) 565
BtsI GCAGTG 3 cut(s) 486, 498, 608
BtsIMutI CAGTG 3 cut(s) 486, 498, 608
Cac8I GCNNGC 5 cut(s) 147, 595, 713, 717, 778
Cfr13I GGNCC 4 cut(s) 322, 398, 819, 980
CviAII CATG 5 cut(s) 37, 256, 704, 893, 955
DdeI CTNAG 2 cut(s) 233, 589
DpnI GATC 4 cut(s) 207, 390, 645, 887
DpnII GATC 4 cut(s) 205, 388, 643, 885
EaeI YGGCCR 1 cut(s) 709
Eam1104I CTCTTC 3 cut(s) 37, 829, 1013
EarI CTCTTC 3 cut(s) 37, 829, 1013
Ecl136II GAGCTC 1 cut(s) 221
Eco130I CCWWGG 3 cut(s) 126, 214, 789
Eco24I GRGCYC 2 cut(s) 223, 1008
Eco47I GGWCC 3 cut(s) 322, 398, 980
Eco53kI GAGCTC 1 cut(s) 221
EcoICRI GAGCTC 1 cut(s) 221
EcoNI CCTNNNNNAGG 1 cut(s) 213
EcoRI GAATTC 1 cut(s) 183
EcoRII CCWGG 2 cut(s) 317, 922
EcoT14I CCWWGG 3 cut(s) 126, 214, 789
EcoT38I GRGCYC 2 cut(s) 223, 1008
ErhI CCWWGG 3 cut(s) 126, 214, 789
Esp3I CGTCTC 1 cut(s) 203
FaeI CATG 5 cut(s) 40, 259, 707, 896, 958
FaqI GGGAC 2 cut(s) 848, 922
FatI CATG 5 cut(s) 36, 255, 703, 892, 954
FbaI TGATCA 1 cut(s) 885
Fnu4HI GCNGC 2 cut(s) 87, 781
FriOI GRGCYC 2 cut(s) 223, 1008
Fsp4HI GCNGC 2 cut(s) 87, 781
FspBI CTAG 3 cut(s) 68, 297, 767
GluI GCNGC 2 cut(s) 87, 781
HaeIII GGCC 2 cut(s) 711, 820
HapII CCGG 1 cut(s) 828
Hin1II CATG 5 cut(s) 40, 259, 707, 896, 958
HinfI GANTC 5 cut(s) 460, 795, 809, 910, 967
HpaII CCGG 1 cut(s) 828
HphI GGTGA 1 cut(s) 684
Hpy166II GTNNAC 2 cut(s) 401, 757
Hpy188I TCNGA 4 cut(s) 189, 457, 657, 808
Hpy188III TCNNGA 3 cut(s) 284, 746, 983
Hpy8I GTNNAC 2 cut(s) 401, 757
HpyAV CCTTC 3 cut(s) 290, 595, 892
HpyCH4III ACNGT 2 cut(s) 653, 754
HpyCH4V TGCA 3 cut(s) 425, 703, 863
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 227, 590
HpyF3I CTNAG 2 cut(s) 233, 589
Hsp92II CATG 5 cut(s) 40, 259, 707, 896, 958
Ksp22I TGATCA 1 cut(s) 885
Kzo9I GATC 4 cut(s) 205, 388, 643, 885
LguI GCTCTTC 1 cut(s) 1013
LmnI GCTCC 3 cut(s) 218, 245, 539
Lsp1109I GCAGC 2 cut(s) 98, 792
LweI GCATC 2 cut(s) 416, 727
MaeI CTAG 3 cut(s) 68, 297, 767
MaeIII GTNAC 1 cut(s) 844
MalI GATC 4 cut(s) 207, 390, 645, 887
MboI GATC 4 cut(s) 205, 388, 643, 885
MboII GAAGA 4 cut(s) 24, 285, 816, 1000
MfeI CAATTG 1 cut(s) 852
MflI RGATCY 1 cut(s) 388
MhlI GDGCHC 2 cut(s) 223, 1008
MlsI TGGCCA 1 cut(s) 711
MluCI AATT 9 cut(s) 183, 313, 355, 447, 474, 852, 867, 880, 1048
MluNI TGGCCA 1 cut(s) 711
MlyI GAGTC 2 cut(s) 818, 904
MmeI TCCRAC 1 cut(s) 267
MnlI CCTC 7 cut(s) 230, 244, 518, 529, 537, 832, 931
Mox20I TGGCCA 1 cut(s) 711
MroXI GAANNNNTTC 2 cut(s) 279, 435
MscI TGGCCA 1 cut(s) 711
MseI TTAA 6 cut(s) 477, 570, 618, 660, 960, 1051
Msp20I TGGCCA 1 cut(s) 711
MspA1I CMGCKG 1 cut(s) 783
MspI CCGG 1 cut(s) 828
MspR9I CCNGG 2 cut(s) 319, 924
MunI CAATTG 1 cut(s) 852
Mva1269I GAATGC 2 cut(s) 542, 865
MvaI CCWGG 2 cut(s) 319, 924
MwoI GCNNNNNNNGC 3 cut(s) 83, 227, 590
NdeII GATC 4 cut(s) 205, 388, 643, 885
NlaIII CATG 5 cut(s) 40, 259, 707, 896, 958
NlaIV GGNNCC 5 cut(s) 100, 124, 241, 323, 787
NmeAIII GCCGAG 1 cut(s) 740
NmuCI GTSAC 1 cut(s) 844
PciSI GCTCTTC 1 cut(s) 1013
PctI GAATGC 2 cut(s) 542, 865
PdmI GAANNNNTTC 2 cut(s) 279, 435
PfeI GAWTC 3 cut(s) 460, 795, 967
PkrI GCNGC 2 cut(s) 88, 782
PleI GAGTC 2 cut(s) 817, 904
PpsI GAGTC 2 cut(s) 817, 904
PshBI ATTAAT 1 cut(s) 660
Psp124BI GAGCTC 1 cut(s) 223
Psp6I CCWGG 2 cut(s) 317, 922
PspGI CCWGG 2 cut(s) 317, 922
PspN4I GGNNCC 5 cut(s) 100, 124, 241, 323, 787
PspPI GGNCC 4 cut(s) 322, 398, 819, 980
PsuI RGATCY 1 cut(s) 388
SacI GAGCTC 1 cut(s) 223
SapI GCTCTTC 1 cut(s) 1013
SaqAI TTAA 6 cut(s) 477, 570, 618, 660, 960, 1051
SatI GCNGC 2 cut(s) 87, 781
Sau3AI GATC 4 cut(s) 205, 388, 643, 885
Sau96I GGNCC 4 cut(s) 322, 398, 819, 980
SchI GAGTC 2 cut(s) 818, 904
ScrFI CCNGG 2 cut(s) 319, 924
SduI GDGCHC 2 cut(s) 223, 1008
SfaNI GCATC 2 cut(s) 416, 727
SfcI CTRYAG 3 cut(s) 93, 393, 933
SinI GGWCC 3 cut(s) 322, 398, 980
SpeI ACTAGT 1 cut(s) 67
Sse9I AATT 9 cut(s) 183, 313, 355, 447, 474, 852, 867, 880, 1048
SsiI CCGC 2 cut(s) 143, 783
SspMI CTAG 3 cut(s) 68, 297, 767
SstI GAGCTC 1 cut(s) 223
StyD4I CCNGG 2 cut(s) 317, 922
StyI CCWWGG 3 cut(s) 126, 214, 789
TaaI ACNGT 2 cut(s) 653, 754
TaqI TCGA 5 cut(s) 171, 249, 552, 666, 747
TasI AATT 9 cut(s) 183, 313, 355, 447, 474, 852, 867, 880, 1048
TfiI GAWTC 3 cut(s) 460, 795, 967
Tru1I TTAA 6 cut(s) 477, 570, 618, 660, 960, 1051
Tru9I TTAA 6 cut(s) 477, 570, 618, 660, 960, 1051
TscAI CASTG 3 cut(s) 493, 505, 615
TseFI GTSAC 1 cut(s) 844
TseI GCWGC 2 cut(s) 86, 780
Tsp45I GTSAC 1 cut(s) 844
TspDTI ATGAA 1 cut(s) 288
TspGWI ACGGA 4 cut(s) 185, 536, 552, 852
TspRI CASTG 3 cut(s) 493, 505, 615
VpaK11BI GGWCC 3 cut(s) 322, 398, 980
VspI ATTAAT 1 cut(s) 660
XagI CCTNNNNNAGG 1 cut(s) 213
XapI RAATTY 2 cut(s) 183, 867
XmnI GAANNNNTTC 2 cut(s) 279, 435
XspI CTAG 3 cut(s) 68, 297, 767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.