pycom10g23030

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001046
Physical Location & Seq
Forward (+)
25280773 .. 25283710
2938 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g23030.1

Sequence Viewer

Length: 387 bp
ATGGCTGGGTACAGAGCTGAGGACGACTACGACTACCTTTTCAAGGTGGTGCTGATCGGCGACTCCGGCGTCGGCAAGTCCAACTTGCTGTCGAGGTTCACCAGGAACGAGTTCAGCCTCGAGTCTAAGTCCACCATCGGAGTCGAGTTCGCCACCCGCAGCTTGGATGTCGACGGCAAAGTCATCAAGGCCCAGATTTGGGACACTGCTGGCCAAGAAAGGTACCGGGCAATAACAAGTGCTTACTATCGAGGGGCTGTGGGTGCACTGCTTGTGTATGATGTCACTCGGAATTCAACATTTGAAAGTGTGGAAAGGTGGTTAAAGGAGTTAAGAGACCACGTGACTGATGATCGATCCCAACATTGTCGTCATGCTTGTCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04961 ko04962 ko04972 map04144 map04961 map04962 map04972
Pfam Domains
Protein Families
Ras

Protein Analysis

129

Amino Acids

14.62

Weight (kDa)

6.83

Isoelectric Point (pI)

29.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 14 - 112 1.1e-08 ADP-ribosylation factor family
Ras PF00071 15 - 117 1.2e-41 Ras family
Roc PF08477 15 - 117 1.3e-31 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 15 - 111 2e-06 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 68, 179
Acc65I GGTACC 1 cut(s) 222
AccB1I GGYRCC 1 cut(s) 222
AccI GTMKAC 1 cut(s) 171
AciI CCGC 1 cut(s) 157
AclWI GGATC 1 cut(s) 351
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 1 cut(s) 292
AcvI CACGTG 1 cut(s) 343
AcyI GRCGYC 1 cut(s) 69
AfaI GTAC 2 cut(s) 11, 224
AfiI CCNNNNNNNGG 4 cut(s) 43, 163, 198, 199
AgsI TTSAA 3 cut(s) 43, 297, 305
AjnI CCWGG 1 cut(s) 101
AluBI AGCT 2 cut(s) 17, 162
AluI AGCT 2 cut(s) 17, 162
Alw21I GWGCWC 1 cut(s) 268
Alw26I GTCTC 1 cut(s) 330
Alw44I GTGCAC 1 cut(s) 264
AlwI GGATC 1 cut(s) 351
Ama87I CYCGRG 1 cut(s) 119
AoxI GGCC 2 cut(s) 189, 211
ApaLI GTGCAC 1 cut(s) 264
ApeKI GCWGC 1 cut(s) 159
ApoI RAATTY 1 cut(s) 292
Asp700I GAANNNNTTC 1 cut(s) 110
Asp718I GGTACC 1 cut(s) 222
AspS9I GGNCC 1 cut(s) 190
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 1 cut(s) 91
AvaI CYCGRG 1 cut(s) 119
BaeGI GKGCMC 1 cut(s) 268
BalI TGGCCA 1 cut(s) 213
BanI GGYRCC 1 cut(s) 222
BbrPI CACGTG 1 cut(s) 343
Bbv12I GWGCWC 1 cut(s) 268
BbvCI CCTCAGC 1 cut(s) 18
BbvI GCAGC 1 cut(s) 171
BccI CCATC 1 cut(s) 143
BceAI ACGGC 1 cut(s) 190
BciT130I CCWGG 1 cut(s) 103
BcnI CCSGG 1 cut(s) 227
BcoDI GTCTC 1 cut(s) 330
BisI GCNGC 1 cut(s) 160
BlsI GCNGC 1 cut(s) 161
Bme1390I CCNGG 2 cut(s) 103, 227
BmeT110I CYCGRG 1 cut(s) 119
BmgT120I GGNCC 1 cut(s) 190
BmiI GGNNCC 1 cut(s) 224
BmrFI CCNGG 2 cut(s) 103, 227
Bpu10I CCTNAGC 1 cut(s) 18
BpuMI CCSGG 1 cut(s) 227
Bsa29I ATCGAT 1 cut(s) 355
BsaAI YACGTR 1 cut(s) 343
BsaHI GRCGYC 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 330
Bsc4I CCNNNNNNNGG 4 cut(s) 43, 163, 198, 199
BseBI CCWGG 1 cut(s) 103
BseCI ATCGAT 1 cut(s) 355
BseGI GGATG 1 cut(s) 172
BseLI CCNNNNNNNGG 4 cut(s) 43, 163, 198, 199
BseMII CTCAG 1 cut(s) 9
BseSI GKGCMC 1 cut(s) 268
BseXI GCAGC 1 cut(s) 171
BseYI CCCAGC 1 cut(s) 5
BshFI GGCC 2 cut(s) 191, 213
BshNI GGYRCC 1 cut(s) 222
BshVI ATCGAT 1 cut(s) 355
BsiHKAI GWGCWC 1 cut(s) 268
BsiHKCI CYCGRG 1 cut(s) 119
BsiSI CCGG 2 cut(s) 66, 226
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 4 cut(s) 43, 163, 198, 199
BsmAI GTCTC 1 cut(s) 330
BsmFI GGGAC 1 cut(s) 215
BsnI GGCC 2 cut(s) 191, 213
Bso31I GGTCTC 1 cut(s) 330
BsoBI CYCGRG 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 268
Bsp143I GATC 3 cut(s) 54, 352, 356
BspACI CCGC 1 cut(s) 157
BspANI GGCC 2 cut(s) 191, 213
BspCNI CTCAG 1 cut(s) 10
BspDI ATCGAT 1 cut(s) 355
BspLI GGNNCC 1 cut(s) 224
BspPI GGATC 1 cut(s) 351
BspT107I GGYRCC 1 cut(s) 222
BspTNI GGTCTC 1 cut(s) 330
BssMI GATC 3 cut(s) 54, 352, 356
BssNI GRCGYC 1 cut(s) 69
Bst2UI CCWGG 1 cut(s) 103
BstACI GRCGYC 1 cut(s) 69
BstBAI YACGTR 1 cut(s) 343
BstC8I GCNNGC 1 cut(s) 211
BstDEI CTNAG 2 cut(s) 18, 126
BstENI CCTNNNNNAGG 1 cut(s) 41
BstF5I GGATG 1 cut(s) 172
BstKTI GATC 3 cut(s) 57, 355, 359
BstMAI GTCTC 1 cut(s) 330
BstMBI GATC 3 cut(s) 54, 352, 356
BstMWI GCNNNNNNNGC 2 cut(s) 66, 263
BstNI CCWGG 1 cut(s) 103
BstSCI CCNGG 2 cut(s) 101, 225
BstSLI GKGCMC 1 cut(s) 268
BstV1I GCAGC 1 cut(s) 171
Bsu15I ATCGAT 1 cut(s) 355
BsuRI GGCC 2 cut(s) 191, 213
BsuTUI ATCGAT 1 cut(s) 355
BtsCI GGATG 1 cut(s) 172
BtsI GCAGTG 2 cut(s) 204, 266
BtsIMutI CAGTG 2 cut(s) 204, 266
Cac8I GCNNGC 1 cut(s) 211
Cfr13I GGNCC 1 cut(s) 190
ClaI ATCGAT 1 cut(s) 355
CseI GACGC 1 cut(s) 58
Csp6I GTAC 2 cut(s) 10, 223
CviAII CATG 1 cut(s) 374
CviJI RGCY 7 cut(s) 5, 17, 117, 162, 191, 213, 257
CviKI_1 RGCY 7 cut(s) 5, 17, 117, 162, 191, 213, 257
CviQI GTAC 2 cut(s) 10, 223
DdeI CTNAG 2 cut(s) 18, 126
DpnI GATC 3 cut(s) 56, 354, 358
DpnII GATC 3 cut(s) 54, 352, 356
DrdI GACNNNNNNGTC 2 cut(s) 68, 179
DseDI GACNNNNNNGTC 2 cut(s) 68, 179
EaeI YGGCCR 1 cut(s) 211
Eco31I GGTCTC 1 cut(s) 330
Eco72I CACGTG 1 cut(s) 343
Eco88I CYCGRG 1 cut(s) 119
EcoNI CCTNNNNNAGG 1 cut(s) 41
EcoRI GAATTC 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 101
FaeI CATG 1 cut(s) 377
FaiI YATR 2 cut(s) 279, 375
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FaqI GGGAC 1 cut(s) 215
FatI CATG 1 cut(s) 373
FauI CCCGC 1 cut(s) 164
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 1 cut(s) 160
FokI GGATG 1 cut(s) 179
Fsp4HI GCNGC 1 cut(s) 160
GluI GCNGC 1 cut(s) 160
GsaI CCCAGC 1 cut(s) 9
HaeIII GGCC 2 cut(s) 191, 213
HapII CCGG 2 cut(s) 66, 226
HgaI GACGC 1 cut(s) 58
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 1 cut(s) 377
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 3 cut(s) 62, 122, 141
HpaII CCGG 2 cut(s) 66, 226
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 4 cut(s) 99, 132, 172, 266
Hpy188I TCNGA 2 cut(s) 140, 291
Hpy8I GTNNAC 4 cut(s) 99, 132, 172, 266
Hpy99I CGWCG 2 cut(s) 74, 176
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 1 cut(s) 266
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 263
HpyF3I CTNAG 2 cut(s) 18, 126
HpySE526I ACGT 1 cut(s) 342
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 1 cut(s) 377
KpnI GGTACC 1 cut(s) 226
Kzo9I GATC 3 cut(s) 54, 352, 356
LpnPI CCDG 6 cut(s) 79, 88, 115, 195, 206, 239
Lsp1109I GCAGC 1 cut(s) 171
MaeII ACGT 1 cut(s) 342
MaeIII GTNAC 2 cut(s) 283, 343
MalI GATC 3 cut(s) 56, 354, 358
MboI GATC 3 cut(s) 54, 352, 356
MhlI GDGCHC 1 cut(s) 268
MlsI TGGCCA 1 cut(s) 213
MluCI AATT 1 cut(s) 292
MluNI TGGCCA 1 cut(s) 213
MlyI GAGTC 3 cut(s) 56, 131, 150
MmeI TCCRAC 1 cut(s) 105
MnlI CCTC 4 cut(s) 13, 87, 128, 245
Mox20I TGGCCA 1 cut(s) 213
MroXI GAANNNNTTC 1 cut(s) 110
MscI TGGCCA 1 cut(s) 213
MseI TTAA 2 cut(s) 323, 332
Msp20I TGGCCA 1 cut(s) 213
MspI CCGG 2 cut(s) 66, 226
MspR9I CCNGG 2 cut(s) 103, 227
MvaI CCWGG 1 cut(s) 103
MwoI GCNNNNNNNGC 2 cut(s) 66, 263
NciI CCSGG 1 cut(s) 227
NdeII GATC 3 cut(s) 54, 352, 356
NlaIII CATG 1 cut(s) 377
NlaIV GGNNCC 1 cut(s) 224
NmuCI GTSAC 2 cut(s) 283, 343
PaeR7I CTCGAG 1 cut(s) 119
PdmI GAANNNNTTC 1 cut(s) 110
PkrI GCNGC 1 cut(s) 161
PleI GAGTC 3 cut(s) 56, 130, 149
PmaCI CACGTG 1 cut(s) 343
PmlI CACGTG 1 cut(s) 343
PpsI GAGTC 3 cut(s) 56, 130, 149
Ppu21I YACGTR 1 cut(s) 343
Psp6I CCWGG 1 cut(s) 101
PspCI CACGTG 1 cut(s) 343
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 1 cut(s) 101
PspN4I GGNNCC 1 cut(s) 224
PspPI GGNCC 1 cut(s) 190
PspXI VCTCGAGB 1 cut(s) 119
RsaI GTAC 2 cut(s) 11, 224
RsaNI GTAC 2 cut(s) 10, 223
SalI GTCGAC 1 cut(s) 170
SaqAI TTAA 2 cut(s) 323, 332
SatI GCNGC 1 cut(s) 160
Sau3AI GATC 3 cut(s) 54, 352, 356
Sau96I GGNCC 1 cut(s) 190
SchI GAGTC 3 cut(s) 56, 131, 150
ScrFI CCNGG 2 cut(s) 103, 227
SduI GDGCHC 1 cut(s) 268
SetI ASST 8 cut(s) 19, 39, 48, 98, 164, 224, 320, 345
Sfr274I CTCGAG 1 cut(s) 119
SlaI CTCGAG 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
Sse9I AATT 1 cut(s) 292
SsiI CCGC 1 cut(s) 157
StyD4I CCNGG 2 cut(s) 101, 225
TaiI ACGT 1 cut(s) 345
TaqI TCGA 6 cut(s) 92, 120, 144, 171, 250, 355
TasI AATT 1 cut(s) 292
Tru1I TTAA 2 cut(s) 323, 332
Tru9I TTAA 2 cut(s) 323, 332
TscAI CASTG 2 cut(s) 211, 273
TseFI GTSAC 2 cut(s) 283, 343
TseI GCWGC 1 cut(s) 159
Tsp45I GTSAC 2 cut(s) 283, 343
TspRI CASTG 2 cut(s) 211, 273
VneI GTGCAC 1 cut(s) 264
XagI CCTNNNNNAGG 1 cut(s) 41
XapI RAATTY 1 cut(s) 292
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
XhoI CTCGAG 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 171
XmnI GAANNNNTTC 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.