RLG00000027792

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001046
Physical Location & Seq
Forward (+)
15297162 .. 15299440
2279 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027792

Sequence Viewer

Length: 465 bp
ATGACGACAAGGCCCGCGGCCTTTTCATCTCCAACCGGAAAGTCTCTCTGGCTCTTCGACATGGCCTACAGGTCGGACGACGACTACCACTTCAAGGTCGTCTTGATAGGCGATTCCGGCCTCTGCAAATCTGATTTGTTGTCCAGGTTCATGCGCAACGAGTTCAACCTCGAGTCTAAGTCCACCATCGGTGTCGAGTTTCCACCCAATTTGGATCACGACGGTGCTACTCCGTTGGGGAGTGTTGCGGATTGGGATCCAGGATTTTTTCAGGTAGTCCACTTTGCTAATCCTTTGGTTCCATTTAATAACAAGAAGTTGGATCACTACATTTATGGTGTTGTTAGTGGGACTGAAGGTGCTTTGATTCATGGTATGAGCGTCGCTAGTGTGCCTAAGAACGACAAAAAGGGTAATGTTGGTATAGATGCTTCTAATGGGGATGGTGGCCCACGCTTTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04961 ko04962 ko04972 map04144 map04961 map04962 map04972
Pfam Domains
Protein Families
Ras

Protein Analysis

155

Amino Acids

16.77

Weight (kDa)

5.64

Isoelectric Point (pI)

30.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ras PF00071 32 - 67 7e-08 Ras family
Roc PF08477 32 - 68 2.4e-07 Ras of Complex, Roc, domain of DAPkinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 155
AccII CGCG 1 cut(s) 17
AciI CCGC 3 cut(s) 15, 17, 248
AclWI GGATC 4 cut(s) 222, 251, 264, 330
AcuI CTGAAG 1 cut(s) 375
AfiI CCNNNNNNNGG 1 cut(s) 94
AgsI TTSAA 2 cut(s) 94, 166
AjnI CCWGG 2 cut(s) 143, 259
AleI CACNNNNGTG 1 cut(s) 222
Alw26I GTCTC 1 cut(s) 48
AlwI GGATC 4 cut(s) 222, 251, 264, 330
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 5 cut(s) 11, 18, 63, 118, 448
AspLEI GCGC 1 cut(s) 156
AspS9I GGNCC 2 cut(s) 12, 449
AvaI CYCGRG 1 cut(s) 170
BamHI GGATCC 1 cut(s) 256
BarI GAAGNNNNNNTAC 2 cut(s) 415, 447
BccI CCATC 2 cut(s) 194, 437
BciT130I CCWGG 2 cut(s) 145, 261
BcoDI GTCTC 1 cut(s) 48
BfaI CTAG 1 cut(s) 387
BfmI CTRYAG 1 cut(s) 67
BisI GCNGC 1 cut(s) 18
BlsI GCNGC 1 cut(s) 19
Bme1390I CCNGG 2 cut(s) 145, 261
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 2 cut(s) 12, 449
BmiI GGNNCC 2 cut(s) 258, 300
BmrFI CCNGG 2 cut(s) 145, 261
BmsI GCATC 1 cut(s) 418
BsaBI GATNNNNATC 1 cut(s) 255
BsaJI CCNNGG 1 cut(s) 15
BsaWI WCCGGW 1 cut(s) 35
Bsc4I CCNNNNNNNGG 1 cut(s) 94
Bse8I GATNNNNATC 1 cut(s) 255
BseBI CCWGG 2 cut(s) 145, 261
BseDI CCNNGG 1 cut(s) 15
BseGI GGATG 1 cut(s) 448
BseJI GATNNNNATC 1 cut(s) 255
BseLI CCNNNNNNNGG 1 cut(s) 94
Bsh1236I CGCG 1 cut(s) 17
BshFI GGCC 5 cut(s) 13, 20, 65, 120, 450
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 2 cut(s) 36, 117
BslFI GGGAC 1 cut(s) 364
BslI CCNNNNNNNGG 1 cut(s) 94
BsmAI GTCTC 1 cut(s) 48
BsmFI GGGAC 1 cut(s) 364
BsnI GGCC 5 cut(s) 13, 20, 65, 120, 450
BsoBI CYCGRG 1 cut(s) 170
Bsp143I GATC 3 cut(s) 214, 256, 322
BspACI CCGC 3 cut(s) 15, 17, 248
BspANI GGCC 5 cut(s) 13, 20, 65, 120, 450
BspFNI CGCG 1 cut(s) 17
BspLI GGNNCC 2 cut(s) 258, 300
BspPI GGATC 4 cut(s) 222, 251, 264, 330
BspQI GCTCTTC 1 cut(s) 59
BssECI CCNNGG 1 cut(s) 15
BssMI GATC 3 cut(s) 214, 256, 322
Bst2UI CCWGG 2 cut(s) 145, 261
Bst4CI ACNGT 1 cut(s) 224
Bst6I CTCTTC 1 cut(s) 59
BstC8I GCNNGC 1 cut(s) 15
BstDEI CTNAG 2 cut(s) 177, 396
BstDSI CCRYGG 1 cut(s) 15
BstF5I GGATG 1 cut(s) 448
BstFNI CGCG 1 cut(s) 17
BstHHI GCGC 1 cut(s) 156
BstKTI GATC 3 cut(s) 217, 259, 325
BstMAI GTCTC 1 cut(s) 48
BstMBI GATC 3 cut(s) 214, 256, 322
BstMWI GCNNNNNNNGC 1 cut(s) 117
BstNI CCWGG 2 cut(s) 145, 261
BstSCI CCNGG 2 cut(s) 143, 259
BstSFI CTRYAG 1 cut(s) 67
BstUI CGCG 1 cut(s) 17
BstX2I RGATCY 1 cut(s) 256
BstYI RGATCY 1 cut(s) 256
BsuRI GGCC 5 cut(s) 13, 20, 65, 120, 450
BtgI CCRYGG 1 cut(s) 15
BtsCI GGATG 1 cut(s) 448
Cac8I GCNNGC 1 cut(s) 15
CfoI GCGC 1 cut(s) 156
Cfr13I GGNCC 2 cut(s) 12, 449
Cfr42I CCGCGG 1 cut(s) 18
CseI GACGC 1 cut(s) 370
CspCI CAANNNNNGTGG 2 cut(s) 192, 227
CviAII CATG 3 cut(s) 61, 151, 371
CviJI RGCY 6 cut(s) 13, 20, 52, 65, 120, 450
CviKI_1 RGCY 6 cut(s) 13, 20, 52, 65, 120, 450
DdeI CTNAG 2 cut(s) 177, 396
DpnI GATC 3 cut(s) 216, 258, 324
DpnII GATC 3 cut(s) 214, 256, 322
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Eco57I CTGAAG 1 cut(s) 375
Eco88I CYCGRG 1 cut(s) 170
EcoRII CCWGG 2 cut(s) 143, 259
FaeI CATG 3 cut(s) 64, 154, 374
FaiI YATR 6 cut(s) 62, 152, 336, 372, 377, 425
FalI AAGNNNNNCTT 2 cut(s) 86, 118
FaqI GGGAC 1 cut(s) 364
FatI CATG 3 cut(s) 60, 150, 370
FauI CCCGC 1 cut(s) 22
Fnu4HI GCNGC 1 cut(s) 18
FokI GGATG 1 cut(s) 455
Fsp4HI GCNGC 1 cut(s) 18
FspBI CTAG 1 cut(s) 387
FspI TGCGCA 1 cut(s) 155
GlaI GCGC 1 cut(s) 155
GluI GCNGC 1 cut(s) 18
HaeIII GGCC 5 cut(s) 13, 20, 65, 120, 450
HapII CCGG 2 cut(s) 36, 117
HgaI GACGC 1 cut(s) 370
HhaI GCGC 1 cut(s) 156
Hin1II CATG 3 cut(s) 64, 154, 374
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HinfI GANTC 3 cut(s) 113, 173, 367
HpaII CCGG 2 cut(s) 36, 117
Hpy166II GTNNAC 2 cut(s) 183, 280
Hpy188I TCNGA 2 cut(s) 76, 133
Hpy188III TCNNGA 2 cut(s) 103, 218
Hpy8I GTNNAC 2 cut(s) 183, 280
Hpy99I CGWCG 3 cut(s) 83, 224, 386
HpyAV CCTTC 1 cut(s) 350
HpyCH4III ACNGT 1 cut(s) 224
HpyCH4V TGCA 1 cut(s) 126
HpyF10VI GCNNNNNNNGC 1 cut(s) 117
HpyF3I CTNAG 2 cut(s) 177, 396
Hsp92II CATG 3 cut(s) 64, 154, 374
HspAI GCGC 1 cut(s) 154
KspI CCGCGG 1 cut(s) 18
Kzo9I GATC 3 cut(s) 214, 256, 322
LguI GCTCTTC 1 cut(s) 59
LpnPI CCDG 9 cut(s) 34, 49, 55, 130, 130, 157, 246, 257, 273
LweI GCATC 1 cut(s) 418
MaeI CTAG 1 cut(s) 387
MalI GATC 3 cut(s) 216, 258, 324
MboI GATC 3 cut(s) 214, 256, 322
MboII GAAGA 1 cut(s) 46
MflI RGATCY 1 cut(s) 256
MluCI AATT 2 cut(s) 208, 460
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 3 cut(s) 54, 56, 300
MnlI CCTC 2 cut(s) 131, 179
MseI TTAA 2 cut(s) 306, 459
MslI CAYNNNNRTG 1 cut(s) 222
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 2 cut(s) 36, 117
MspR9I CCNGG 2 cut(s) 145, 261
MvaI CCWGG 2 cut(s) 145, 261
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 1 cut(s) 117
NdeII GATC 3 cut(s) 214, 256, 322
NlaIII CATG 3 cut(s) 64, 154, 374
NlaIV GGNNCC 2 cut(s) 258, 300
NsbI TGCGCA 1 cut(s) 155
OliI CACNNNNGTG 1 cut(s) 222
PaeR7I CTCGAG 1 cut(s) 170
PciSI GCTCTTC 1 cut(s) 59
PfeI GAWTC 2 cut(s) 113, 367
PfoI TCCNGGA 1 cut(s) 259
PkrI GCNGC 1 cut(s) 19
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
Psp6I CCWGG 2 cut(s) 143, 259
PspGI CCWGG 2 cut(s) 143, 259
PspN4I GGNNCC 2 cut(s) 258, 300
PspPI GGNCC 2 cut(s) 12, 449
PspXI VCTCGAGB 1 cut(s) 170
PsuI RGATCY 1 cut(s) 256
RseI CAYNNNNRTG 1 cut(s) 222
SacII CCGCGG 1 cut(s) 18
SapI GCTCTTC 1 cut(s) 59
SaqAI TTAA 2 cut(s) 306, 459
SatI GCNGC 1 cut(s) 18
Sau3AI GATC 3 cut(s) 214, 256, 322
Sau96I GGNCC 2 cut(s) 12, 449
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 2 cut(s) 145, 261
SetI ASST 6 cut(s) 74, 99, 149, 171, 276, 361
SfaNI GCATC 1 cut(s) 418
SfcI CTRYAG 1 cut(s) 67
Sfr274I CTCGAG 1 cut(s) 170
Sfr303I CCGCGG 1 cut(s) 18
SgrBI CCGCGG 1 cut(s) 18
SlaI CTCGAG 1 cut(s) 170
SmiMI CAYNNNNRTG 1 cut(s) 222
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 2 cut(s) 208, 460
SsiI CCGC 3 cut(s) 15, 17, 248
SspMI CTAG 1 cut(s) 387
StyD4I CCNGG 2 cut(s) 143, 259
TaaI ACNGT 1 cut(s) 224
TaqI TCGA 3 cut(s) 57, 171, 195
TasI AATT 2 cut(s) 208, 460
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 2 cut(s) 113, 367
Tru1I TTAA 2 cut(s) 306, 459
Tru9I TTAA 2 cut(s) 306, 459
TspDTI ATGAA 3 cut(s) 15, 139, 359
TspGWI ACGGA 1 cut(s) 222
XhoI CTCGAG 1 cut(s) 170
XspI CTAG 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.