Protein of unknown function (DUF3754)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 480 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 145 |
| AciI | CCGC | 2 cut(s) | 228, 330 |
| AclWI | GGATC | 1 cut(s) | 259 |
| AdeI | CACNNNGTG | 1 cut(s) | 142 |
| AfaI | GTAC | 2 cut(s) | 209, 238 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 145 |
| AluBI | AGCT | 2 cut(s) | 124, 326 |
| AluI | AGCT | 2 cut(s) | 124, 326 |
| Alw21I | GWGCWC | 1 cut(s) | 84 |
| Alw44I | GTGCAC | 1 cut(s) | 80 |
| AlwI | GGATC | 1 cut(s) | 259 |
| Ama87I | CYCGRG | 1 cut(s) | 444 |
| ApaLI | GTGCAC | 1 cut(s) | 80 |
| ApeKI | GCWGC | 1 cut(s) | 59 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 377, 409 |
| AseI | ATTAAT | 1 cut(s) | 308 |
| AsuHPI | GGTGA | 2 cut(s) | 128, 298 |
| AvaI | CYCGRG | 1 cut(s) | 444 |
| BaeGI | GKGCMC | 1 cut(s) | 84 |
| BanI | GGYRCC | 1 cut(s) | 480 |
| Bbv12I | GWGCWC | 1 cut(s) | 84 |
| BbvI | GCAGC | 1 cut(s) | 71 |
| BclI | TGATCA | 1 cut(s) | 363 |
| BfaI | CTAG | 1 cut(s) | 335 |
| BfmI | CTRYAG | 2 cut(s) | 30, 154 |
| BglII | AGATCT | 1 cut(s) | 337 |
| BisI | GCNGC | 1 cut(s) | 60 |
| BlsI | GCNGC | 1 cut(s) | 61 |
| BmeT110I | CYCGRG | 1 cut(s) | 444 |
| BmiI | GGNNCC | 1 cut(s) | 482 |
| BmsI | GCATC | 1 cut(s) | 397 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 145 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 145 |
| BseMII | CTCAG | 1 cut(s) | 191 |
| BseRI | GAGGAG | 1 cut(s) | 507 |
| BseSI | GKGCMC | 1 cut(s) | 84 |
| BseXI | GCAGC | 1 cut(s) | 71 |
| BshNI | GGYRCC | 1 cut(s) | 480 |
| BsiHKAI | GWGCWC | 1 cut(s) | 84 |
| BsiHKCI | CYCGRG | 1 cut(s) | 444 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 145 |
| BsoBI | CYCGRG | 1 cut(s) | 444 |
| Bsp1286I | GDGCHC | 1 cut(s) | 84 |
| Bsp143I | GATC | 4 cut(s) | 264, 337, 363, 472 |
| BspACI | CCGC | 2 cut(s) | 228, 330 |
| BspCNI | CTCAG | 1 cut(s) | 190 |
| BspLI | GGNNCC | 1 cut(s) | 482 |
| BspMAI | CTGCAG | 1 cut(s) | 158 |
| BspPI | GGATC | 1 cut(s) | 259 |
| BspT107I | GGYRCC | 1 cut(s) | 480 |
| BssMI | GATC | 4 cut(s) | 264, 337, 363, 472 |
| Bst4CI | ACNGT | 4 cut(s) | 86, 201, 375, 389 |
| BstDEI | CTNAG | 1 cut(s) | 177 |
| BstKTI | GATC | 4 cut(s) | 267, 340, 366, 475 |
| BstMBI | GATC | 4 cut(s) | 264, 337, 363, 472 |
| BstSFI | CTRYAG | 2 cut(s) | 30, 154 |
| BstSLI | GKGCMC | 1 cut(s) | 84 |
| BstV1I | GCAGC | 1 cut(s) | 71 |
| BstX2I | RGATCY | 1 cut(s) | 337 |
| BstYI | RGATCY | 1 cut(s) | 337 |
| BtgZI | GCGATG | 1 cut(s) | 270 |
| BtsIMutI | CAGTG | 1 cut(s) | 82 |
| Csp6I | GTAC | 2 cut(s) | 208, 237 |
| CviAII | CATG | 2 cut(s) | 140, 205 |
| CviJI | RGCY | 7 cut(s) | 10, 24, 40, 124, 176, 326, 455 |
| CviKI_1 | RGCY | 7 cut(s) | 10, 24, 40, 124, 176, 326, 455 |
| CviQI | GTAC | 2 cut(s) | 208, 237 |
| DdeI | CTNAG | 1 cut(s) | 177 |
| DpnI | GATC | 4 cut(s) | 266, 339, 365, 474 |
| DpnII | GATC | 4 cut(s) | 264, 337, 363, 472 |
| DraIII | CACNNNGTG | 1 cut(s) | 142 |
| Eco88I | CYCGRG | 1 cut(s) | 444 |
| FaeI | CATG | 2 cut(s) | 143, 208 |
| FaiI | YATR | 3 cut(s) | 141, 206, 305 |
| FatI | CATG | 2 cut(s) | 139, 204 |
| FbaI | TGATCA | 1 cut(s) | 363 |
| Fnu4HI | GCNGC | 1 cut(s) | 60 |
| Fsp4HI | GCNGC | 1 cut(s) | 60 |
| FspBI | CTAG | 1 cut(s) | 335 |
| GluI | GCNGC | 1 cut(s) | 60 |
| Hin1II | CATG | 2 cut(s) | 143, 208 |
| HphI | GGTGA | 2 cut(s) | 128, 298 |
| Hpy166II | GTNNAC | 3 cut(s) | 82, 151, 403 |
| Hpy188I | TCNGA | 2 cut(s) | 186, 417 |
| Hpy188III | TCNNGA | 2 cut(s) | 52, 341 |
| Hpy8I | GTNNAC | 3 cut(s) | 82, 151, 403 |
| Hpy99I | CGWCG | 1 cut(s) | 386 |
| HpyAV | CCTTC | 2 cut(s) | 254, 274 |
| HpyCH4III | ACNGT | 4 cut(s) | 86, 201, 375, 389 |
| HpyCH4IV | ACGT | 2 cut(s) | 14, 461 |
| HpyCH4V | TGCA | 3 cut(s) | 82, 156, 410 |
| HpyF3I | CTNAG | 1 cut(s) | 177 |
| HpySE526I | ACGT | 2 cut(s) | 14, 461 |
| Hsp92II | CATG | 2 cut(s) | 143, 208 |
| Ksp22I | TGATCA | 1 cut(s) | 363 |
| Kzo9I | GATC | 4 cut(s) | 264, 337, 363, 472 |
| LpnPI | CCDG | 3 cut(s) | 26, 257, 326 |
| Lsp1109I | GCAGC | 1 cut(s) | 71 |
| LweI | GCATC | 1 cut(s) | 397 |
| MaeI | CTAG | 1 cut(s) | 335 |
| MaeII | ACGT | 2 cut(s) | 14, 461 |
| MaeIII | GTNAC | 1 cut(s) | 439 |
| MalI | GATC | 4 cut(s) | 266, 339, 365, 474 |
| MboI | GATC | 4 cut(s) | 264, 337, 363, 472 |
| MfeI | CAATTG | 1 cut(s) | 218 |
| MflI | RGATCY | 1 cut(s) | 337 |
| MhlI | GDGCHC | 1 cut(s) | 84 |
| MluCI | AATT | 5 cut(s) | 218, 291, 391, 428, 435 |
| MmeI | TCCRAC | 2 cut(s) | 292, 402 |
| MnlI | CCTC | 4 cut(s) | 277, 349, 485, 488 |
| MseI | TTAA | 5 cut(s) | 105, 126, 308, 378, 505 |
| MslI | CAYNNNNRTG | 1 cut(s) | 308 |
| MunI | CAATTG | 1 cut(s) | 218 |
| NdeII | GATC | 4 cut(s) | 264, 337, 363, 472 |
| NlaIII | CATG | 2 cut(s) | 143, 208 |
| NlaIV | GGNNCC | 1 cut(s) | 482 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 145 |
| PkrI | GCNGC | 1 cut(s) | 61 |
| PshBI | ATTAAT | 1 cut(s) | 308 |
| PspN4I | GGNNCC | 1 cut(s) | 482 |
| PstI | CTGCAG | 1 cut(s) | 158 |
| PsuI | RGATCY | 1 cut(s) | 337 |
| RsaI | GTAC | 2 cut(s) | 209, 238 |
| RsaNI | GTAC | 2 cut(s) | 208, 237 |
| RseI | CAYNNNNRTG | 1 cut(s) | 308 |
| SaqAI | TTAA | 5 cut(s) | 105, 126, 308, 378, 505 |
| SatI | GCNGC | 1 cut(s) | 60 |
| Sau3AI | GATC | 4 cut(s) | 264, 337, 363, 472 |
| SduI | GDGCHC | 1 cut(s) | 84 |
| SetI | ASST | 6 cut(s) | 17, 126, 288, 328, 464, 499 |
| SfaNI | GCATC | 1 cut(s) | 397 |
| SfcI | CTRYAG | 2 cut(s) | 30, 154 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 308 |
| Sse9I | AATT | 5 cut(s) | 218, 291, 391, 428, 435 |
| SsiI | CCGC | 2 cut(s) | 228, 330 |
| SspMI | CTAG | 1 cut(s) | 335 |
| TaaI | ACNGT | 4 cut(s) | 86, 201, 375, 389 |
| TaiI | ACGT | 2 cut(s) | 17, 464 |
| TasI | AATT | 5 cut(s) | 218, 291, 391, 428, 435 |
| Tru1I | TTAA | 5 cut(s) | 105, 126, 308, 378, 505 |
| Tru9I | TTAA | 5 cut(s) | 105, 126, 308, 378, 505 |
| TscAI | CASTG | 1 cut(s) | 89 |
| TseI | GCWGC | 1 cut(s) | 59 |
| TspRI | CASTG | 1 cut(s) | 89 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 145 |
| VneI | GTGCAC | 1 cut(s) | 80 |
| VspI | ATTAAT | 1 cut(s) | 308 |
| XspI | CTAG | 1 cut(s) | 335 |