pycom17g24240

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000634
Physical Location & Seq
Forward (+)
22546644 .. 22551249
4606 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1392 bp
ATGGAGATTTCGGAAGAGGGACTAGAGCCAGTGAGCCCTACTGGCCATTATTTCAACAACTCTGTTTTATCAATATCAGTTCTTGCTGTTTTGGAATTTGAAATGCCAATTGATGACTCTCAATTTCTGTCATTGCTCGAGAACGTGTTCCTGCAGGTCAACCCACGCTTCTCCTGTATCATGATTGAAAAAAATGGAAAGAAACAATGGAAAAGGGTTGAAGTGAAGCTTGAAGACCATGTTAAAACTCCAACCTTCCCTTCCAACTTGTCGAATGAATCATATGACGAGTATTTTGATGACTATTTGTCAAACTTAGCAACAAAAAGATTTCCACAAGACAAACCACTGTGGGAATTTCATGTTATAAAGTACCCAAATAGCAATGCAGCTGGTAATATAGTATTTAAGTTGCACCATGCGTTAGGTGATGGCCACTCACTCATGGGCGCTCTTCTCTCTTGTCTACAAAGGGCTGACAATCCTTCTCTTCCCCTAACTTTTCCTTCACGGCAGCCATCGAAACCGAAGAATGAAAATGTTATGACCAGAACTTTCTCATCCTTGTTGAACACCATGTCCGATATTTGGTGGGGCATTTCAAAGAGCATCACCGGGGAAGATGATCGATCACCAATAAAATCCTCGAACATTGGATTCGAGTTCGCGCCAATTACAGTATCAACTGTGACAATCTCTCTTGATCACATCAAATTAATTAAAAGCAGGCTTGGAGTGACGATAAATGACGTCCTTACCGGGATGGTCTTTCTTGGCTCTCGACTATATATGCAAGAGATGAACCAAAGCTCAAGGGAAGCCCGTAGCACTGCATTGATTTTACTAAATACAAGGGTCATGGGGAATTATACTTCGGTTGAAGAAATGGTCAAACCCGACAGCAAGAGCCCATGGGGAAATCGTTTTACATTCTTGCAGATTCCCATTCCCAATTTGACTGAGTTCTCAAACGCGCTTGACTTTGTTTGGAAGGCTCAAAAAATTATCAAGACGAAAAGAAATTCTTTAGCTATTTATTTCACTAGTGAGCTATTGAAGATGTTGAACAGGTTTGGAGGCCACGAGGCAGCATCAAGATTCATCCATAGAACATTAACGAACTCGAGCATGGCATTTACAAATTTGATCGGGCCCGTGGAACAAATGTCATTGGCAAACCAACCAATAAAAGGATTATACTTTATCATAGTTGGATCACCTCTGGATTTTGACATATCAGTCCTAAGTTATATGGGAAAAGTGAGGCTTACCTTAACAATGAAAAAAGGCATCATAGATCCACAAAAACTGAAGTTGTGCATGGAAAACGCCCATGATATGATATTAAATGCCTCGGACAAATATCCTGTGCAAAATAGCACCGAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

464

Amino Acids

52.34

Weight (kDa)

8.22

Isoelectric Point (pI)

45.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 68 - 251 4.6e-11 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C PF06974 305 - 448 2.2e-35 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 368
AasI GACNNNNNNGTC 1 cut(s) 1238
AatII GACGTC 1 cut(s) 753
Acc36I ACCTGC 1 cut(s) 145
AccI GTMKAC 1 cut(s) 466
AccII CGCG 2 cut(s) 668, 974
AclWI GGATC 2 cut(s) 1222, 1292
AcoI YGGCCR 2 cut(s) 43, 433
AcsI RAATTY 4 cut(s) 95, 356, 1021, 1141
AcuI CTGAAG 1 cut(s) 1331
AcyI GRCGYC 1 cut(s) 750
AfaI GTAC 1 cut(s) 374
AfiI CCNNNNNNNGG 2 cut(s) 588, 1190
AflIII ACRYGT 1 cut(s) 144
AhdI GACNNNNNGTC 1 cut(s) 307
AhlI ACTAGT 1 cut(s) 1043
AjuI GAANNNNNNNTTGG 2 cut(s) 244, 276
AloI GAACNNNNNNTCC 2 cut(s) 563, 595
AluBI AGCT 5 cut(s) 229, 392, 810, 1031, 1051
AluI AGCT 5 cut(s) 229, 392, 810, 1031, 1051
AlwI GGATC 2 cut(s) 1222, 1292
Ama87I CYCGRG 2 cut(s) 137, 1123
AoxI GGCC 4 cut(s) 43, 433, 1078, 1151
ApaI GGGCCC 1 cut(s) 1155
ApeKI GCWGC 3 cut(s) 389, 514, 1088
ApoI RAATTY 4 cut(s) 95, 356, 1021, 1141
ArsI GACNNNNNNTTYG 2 cut(s) 278, 310
AseI ATTAAT 1 cut(s) 716
Asp700I GAANNNNTTC 1 cut(s) 146
AspLEI GCGC 3 cut(s) 452, 670, 976
AspS9I GGNCC 2 cut(s) 1151, 1152
AsuC2I CCSGG 2 cut(s) 616, 760
AsuHPI GGTGA 4 cut(s) 440, 604, 624, 1209
AvaI CYCGRG 2 cut(s) 137, 1123
BaeGI GKGCMC 1 cut(s) 1155
BalI TGGCCA 2 cut(s) 45, 435
BanII GRGCYC 3 cut(s) 38, 911, 1155
BauI CACGAG 1 cut(s) 1082
BbsI GAAGAC 1 cut(s) 240
BbvI GCAGC 3 cut(s) 401, 526, 1100
BccI CCATC 3 cut(s) 425, 526, 757
BceAI ACGGC 1 cut(s) 527
BclI TGATCA 1 cut(s) 703
BcnI CCSGG 2 cut(s) 616, 760
BcuI ACTAGT 1 cut(s) 1043
BfaI CTAG 2 cut(s) 23, 1044
BfmI CTRYAG 1 cut(s) 152
BfoI RGCGCY 1 cut(s) 453
BfuAI ACCTGC 1 cut(s) 145
BglI GCCNNNNNGGC 1 cut(s) 42
BisI GCNGC 3 cut(s) 390, 515, 1089
BlsI GCNGC 3 cut(s) 391, 516, 1090
Bme1390I CCNGG 2 cut(s) 616, 760
BmeRI GACNNNNNGTC 1 cut(s) 307
BmeT110I CYCGRG 2 cut(s) 137, 1123
BmgT120I GGNCC 2 cut(s) 1151, 1152
BmiI GGNNCC 1 cut(s) 1153
BmrFI CCNGG 2 cut(s) 616, 760
BmsI GCATC 3 cut(s) 618, 1100, 1299
BpiI GAAGAC 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 796
BpuMI CCSGG 2 cut(s) 616, 760
Bsa29I ATCGAT 1 cut(s) 628
BsaHI GRCGYC 1 cut(s) 750
BsaJI CCNNGG 4 cut(s) 615, 911, 1155, 1353
Bsc4I CCNNNNNNNGG 2 cut(s) 588, 1190
Bse1I ACTGG 2 cut(s) 29, 46
Bse3DI GCAATG 2 cut(s) 131, 391
BseCI ATCGAT 1 cut(s) 628
BseDI CCNNGG 4 cut(s) 615, 911, 1155, 1353
BseGI GGATG 3 cut(s) 560, 768, 1101
BseLI CCNNNNNNNGG 2 cut(s) 588, 1190
BseMI GCAATG 2 cut(s) 131, 391
BseMII CTCAG 1 cut(s) 951
BseNI ACTGG 2 cut(s) 29, 46
BseSI GKGCMC 1 cut(s) 1155
BseXI GCAGC 3 cut(s) 401, 526, 1100
Bsh1236I CGCG 2 cut(s) 668, 974
BshFI GGCC 4 cut(s) 45, 435, 1080, 1153
BshVI ATCGAT 1 cut(s) 628
BsiHKCI CYCGRG 2 cut(s) 137, 1123
BsiSI CCGG 2 cut(s) 615, 759
BslFI GGGAC 1 cut(s) 33
BslI CCNNNNNNNGG 2 cut(s) 588, 1190
BsmFI GGGAC 1 cut(s) 33
BsnI GGCC 4 cut(s) 45, 435, 1080, 1153
BsoBI CYCGRG 2 cut(s) 137, 1123
Bsp120I GGGCCC 1 cut(s) 1151
Bsp1286I GDGCHC 3 cut(s) 38, 911, 1155
Bsp143I GATC 6 cut(s) 625, 629, 703, 1146, 1214, 1297
Bsp19I CCATGG 1 cut(s) 911
BspANI GGCC 4 cut(s) 45, 435, 1080, 1153
BspCNI CTCAG 1 cut(s) 952
BspDI ATCGAT 1 cut(s) 628
BspFNI CGCG 2 cut(s) 668, 974
BspHI TCATGA 1 cut(s) 180
BspLI GGNNCC 1 cut(s) 1153
BspMAI CTGCAG 1 cut(s) 156
BspMI ACCTGC 1 cut(s) 145
BspPI GGATC 2 cut(s) 1222, 1292
BspQI GCTCTTC 1 cut(s) 459
BsrDI GCAATG 2 cut(s) 131, 391
BsrI ACTGG 2 cut(s) 29, 46
BssECI CCNNGG 4 cut(s) 615, 911, 1155, 1353
BssMI GATC 6 cut(s) 625, 629, 703, 1146, 1214, 1297
BssNI GRCGYC 1 cut(s) 750
BssSI CACGAG 1 cut(s) 1082
BssT1I CCWWGG 1 cut(s) 911
Bst2BI CACGAG 1 cut(s) 1082
Bst4CI ACNGT 3 cut(s) 351, 679, 688
Bst6I CTCTTC 3 cut(s) 9, 459, 495
BstACI GRCGYC 1 cut(s) 750
BstC8I GCNNGC 1 cut(s) 728
BstDEI CTNAG 3 cut(s) 316, 960, 1244
BstDSI CCRYGG 2 cut(s) 911, 1155
BstF5I GGATG 3 cut(s) 560, 768, 1101
BstFNI CGCG 2 cut(s) 668, 974
BstH2I RGCGCY 1 cut(s) 453
BstHHI GCGC 3 cut(s) 452, 670, 976
BstKTI GATC 6 cut(s) 628, 632, 706, 1149, 1217, 1300
BstMBI GATC 6 cut(s) 625, 629, 703, 1146, 1214, 1297
BstMWI GCNNNNNNNGC 1 cut(s) 42
BstSCI CCNGG 2 cut(s) 614, 758
BstSFI CTRYAG 1 cut(s) 152
BstSLI GKGCMC 1 cut(s) 1155
BstUI CGCG 2 cut(s) 668, 974
BstV1I GCAGC 3 cut(s) 401, 526, 1100
BstV2I GAAGAC 1 cut(s) 240
BstX2I RGATCY 1 cut(s) 1297
BstYI RGATCY 1 cut(s) 1297
Bsu15I ATCGAT 1 cut(s) 628
BsuRI GGCC 4 cut(s) 45, 435, 1080, 1153
BsuTUI ATCGAT 1 cut(s) 628
BtgI CCRYGG 2 cut(s) 911, 1155
BtsCI GGATG 3 cut(s) 560, 768, 1101
BtsI GCAGTG 1 cut(s) 828
BtsIMutI CAGTG 3 cut(s) 36, 347, 828
BveI ACCTGC 1 cut(s) 145
Cac8I GCNNGC 1 cut(s) 728
CciI TCATGA 1 cut(s) 180
CfoI GCGC 3 cut(s) 452, 670, 976
Cfr13I GGNCC 2 cut(s) 1151, 1152
ClaI ATCGAT 1 cut(s) 628
Csp6I GTAC 1 cut(s) 373
CviQI GTAC 1 cut(s) 373
DdeI CTNAG 3 cut(s) 316, 960, 1244
DpnI GATC 6 cut(s) 627, 631, 705, 1148, 1216, 1299
DpnII GATC 6 cut(s) 625, 629, 703, 1146, 1214, 1297
DrdI GACNNNNNNGTC 1 cut(s) 1238
DriI GACNNNNNGTC 1 cut(s) 307
DseDI GACNNNNNNGTC 1 cut(s) 1238
EaeI YGGCCR 2 cut(s) 43, 433
Eam1104I CTCTTC 3 cut(s) 9, 459, 495
Eam1105I GACNNNNNGTC 1 cut(s) 307
EarI CTCTTC 3 cut(s) 9, 459, 495
Eco130I CCWWGG 1 cut(s) 911
Eco24I GRGCYC 3 cut(s) 38, 911, 1155
Eco57I CTGAAG 1 cut(s) 1331
Eco88I CYCGRG 2 cut(s) 137, 1123
EcoT14I CCWWGG 1 cut(s) 911
EcoT38I GRGCYC 3 cut(s) 38, 911, 1155
ErhI CCWWGG 1 cut(s) 911
FalI AAGNNNNNCTT 6 cut(s) 213, 245, 1009, 1041, 1251, 1283
FaqI GGGAC 1 cut(s) 33
FauNDI CATATG 1 cut(s) 283
FbaI TGATCA 1 cut(s) 703
FblI GTMKAC 1 cut(s) 466
Fnu4HI GCNGC 3 cut(s) 390, 515, 1089
FokI GGATG 3 cut(s) 547, 775, 1088
FriOI GRGCYC 3 cut(s) 38, 911, 1155
Fsp4HI GCNGC 3 cut(s) 390, 515, 1089
FspBI CTAG 2 cut(s) 23, 1044
GlaI GCGC 3 cut(s) 451, 669, 975
GluI GCNGC 3 cut(s) 390, 515, 1089
HaeII RGCGCY 1 cut(s) 453
HaeIII GGCC 4 cut(s) 45, 435, 1080, 1153
HapII CCGG 2 cut(s) 615, 759
HhaI GCGC 3 cut(s) 452, 670, 976
Hin1I GRCGYC 1 cut(s) 750
Hin6I GCGC 3 cut(s) 450, 668, 974
HinP1I GCGC 3 cut(s) 450, 668, 974
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 5 cut(s) 116, 278, 657, 940, 1098
HpaII CCGG 2 cut(s) 615, 759
HphI GGTGA 4 cut(s) 440, 604, 624, 1209
Hpy166II GTNNAC 2 cut(s) 160, 467
Hpy188I TCNGA 3 cut(s) 13, 583, 1357
Hpy188III TCNNGA 7 cut(s) 139, 181, 701, 780, 1009, 1095, 1223
Hpy8I GTNNAC 2 cut(s) 160, 467
HpyAV CCTTC 5 cut(s) 265, 270, 495, 516, 985
HpyCH4III ACNGT 3 cut(s) 351, 679, 688
HpyCH4IV ACGT 2 cut(s) 144, 750
HpyCH4V TGCA 8 cut(s) 154, 389, 415, 793, 833, 937, 1320, 1372
HpyF10VI GCNNNNNNNGC 1 cut(s) 42
HpyF3I CTNAG 3 cut(s) 316, 960, 1244
HpySE526I ACGT 2 cut(s) 144, 750
Hsp92I GRCGYC 1 cut(s) 750
HspAI GCGC 3 cut(s) 450, 668, 974
Ksp22I TGATCA 1 cut(s) 703
Kzo9I GATC 6 cut(s) 625, 629, 703, 1146, 1214, 1297
LguI GCTCTTC 1 cut(s) 459
Lsp1109I GCAGC 3 cut(s) 401, 526, 1100
LweI GCATC 3 cut(s) 618, 1100, 1299
MaeI CTAG 2 cut(s) 23, 1044
MaeII ACGT 2 cut(s) 144, 750
MaeIII GTNAC 2 cut(s) 688, 736
MalI GATC 6 cut(s) 627, 631, 705, 1148, 1216, 1299
MboI GATC 6 cut(s) 625, 629, 703, 1146, 1214, 1297
MboII GAAGA 8 cut(s) 26, 245, 446, 482, 541, 632, 893, 1069
MfeI CAATTG 1 cut(s) 108
MflI RGATCY 1 cut(s) 1297
MhlI GDGCHC 3 cut(s) 38, 911, 1155
MlsI TGGCCA 2 cut(s) 45, 435
MluNI TGGCCA 2 cut(s) 45, 435
MlyI GAGTC 1 cut(s) 110
MmeI TCCRAC 3 cut(s) 275, 288, 1192
MnlI CCTC 7 cut(s) 10, 655, 1070, 1078, 1230, 1257, 1363
Mox20I TGGCCA 2 cut(s) 45, 435
MroXI GAANNNNTTC 1 cut(s) 146
MscI TGGCCA 2 cut(s) 45, 435
MseI TTAA 7 cut(s) 243, 408, 716, 720, 1115, 1274, 1346
Msp20I TGGCCA 2 cut(s) 45, 435
MspA1I CMGCKG 1 cut(s) 392
MspI CCGG 2 cut(s) 615, 759
MspR9I CCNGG 2 cut(s) 616, 760
MunI CAATTG 1 cut(s) 108
MvnI CGCG 2 cut(s) 668, 974
MwoI GCNNNNNNNGC 1 cut(s) 42
NciI CCSGG 2 cut(s) 616, 760
NcoI CCATGG 1 cut(s) 911
NdeI CATATG 1 cut(s) 283
NdeII GATC 6 cut(s) 625, 629, 703, 1146, 1214, 1297
NlaIV GGNNCC 1 cut(s) 1153
NmuCI GTSAC 2 cut(s) 688, 736
PacI TTAATTAA 1 cut(s) 720
PaeR7I CTCGAG 2 cut(s) 137, 1123
PagI TCATGA 1 cut(s) 180
PciSI GCTCTTC 1 cut(s) 459
PdmI GAANNNNTTC 1 cut(s) 146
PfeI GAWTC 4 cut(s) 278, 657, 940, 1098
PkrI GCNGC 3 cut(s) 391, 516, 1090
PleI GAGTC 1 cut(s) 110
PpsI GAGTC 1 cut(s) 110
PshBI ATTAAT 1 cut(s) 716
PsiI TTATAA 1 cut(s) 368
PspN4I GGNNCC 1 cut(s) 1153
PspOMI GGGCCC 1 cut(s) 1151
PspPI GGNCC 2 cut(s) 1151, 1152
PspXI VCTCGAGB 1 cut(s) 1123
PstI CTGCAG 1 cut(s) 156
PsuI RGATCY 1 cut(s) 1297
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 1 cut(s) 374
RsaNI GTAC 1 cut(s) 373
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 7 cut(s) 243, 408, 716, 720, 1115, 1274, 1346
SatI GCNGC 3 cut(s) 390, 515, 1089
Sau3AI GATC 6 cut(s) 625, 629, 703, 1146, 1214, 1297
Sau96I GGNCC 2 cut(s) 1151, 1152
SbfI CCTGCAGG 1 cut(s) 156
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 2 cut(s) 616, 760
SdaI CCTGCAGG 1 cut(s) 156
SduI GDGCHC 3 cut(s) 38, 911, 1155
SfaNI GCATC 3 cut(s) 618, 1100, 1299
SfcI CTRYAG 1 cut(s) 152
Sfr274I CTCGAG 2 cut(s) 137, 1123
SlaI CTCGAG 2 cut(s) 137, 1123
SmlI CTYRAG 3 cut(s) 137, 811, 1123
SmoI CTYRAG 3 cut(s) 137, 811, 1123
SpeI ACTAGT 1 cut(s) 1043
Sse8387I CCTGCAGG 1 cut(s) 156
SspMI CTAG 2 cut(s) 23, 1044
StyD4I CCNGG 2 cut(s) 614, 758
StyI CCWWGG 1 cut(s) 911
TaaI ACNGT 3 cut(s) 351, 679, 688
TaiI ACGT 2 cut(s) 147, 753
TaqI TCGA 8 cut(s) 138, 272, 521, 628, 647, 660, 781, 1124
TfiI GAWTC 4 cut(s) 278, 657, 940, 1098
Tru1I TTAA 7 cut(s) 243, 408, 716, 720, 1115, 1274, 1346
Tru9I TTAA 7 cut(s) 243, 408, 716, 720, 1115, 1274, 1346
TscAI CASTG 3 cut(s) 36, 354, 835
TseFI GTSAC 2 cut(s) 688, 736
TseI GCWGC 3 cut(s) 389, 514, 1088
Tsp45I GTSAC 2 cut(s) 688, 736
TspDTI ATGAA 6 cut(s) 291, 350, 549, 815, 1090, 1295
TspRI CASTG 3 cut(s) 36, 354, 835
VspI ATTAAT 1 cut(s) 716
XapI RAATTY 4 cut(s) 95, 356, 1021, 1141
XhoI CTCGAG 2 cut(s) 137, 1123
XmiI GTMKAC 1 cut(s) 466
XmnI GAANNNNTTC 1 cut(s) 146
XspI CTAG 2 cut(s) 23, 1044
ZraI GACGTC 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.