Protein kinase domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 70 |
| AccB1I | GGYRCC | 1 cut(s) | 182 |
| AccI | GTMKAC | 1 cut(s) | 915 |
| AccII | CGCG | 1 cut(s) | 856 |
| AccIII | TCCGGA | 1 cut(s) | 871 |
| AciI | CCGC | 5 cut(s) | 17, 56, 848, 966, 1064 |
| AcsI | RAATTY | 2 cut(s) | 780, 823 |
| AcuI | CTGAAG | 1 cut(s) | 1068 |
| AfaI | GTAC | 3 cut(s) | 457, 652, 1125 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1011 |
| AflIII | ACRYGT | 2 cut(s) | 653, 854 |
| AgsI | TTSAA | 7 cut(s) | 278, 484, 624, 671, 718, 812, 1310 |
| AluBI | AGCT | 5 cut(s) | 215, 415, 600, 991, 1292 |
| AluI | AGCT | 5 cut(s) | 215, 415, 600, 991, 1292 |
| Alw21I | GWGCWC | 1 cut(s) | 1207 |
| Alw26I | GTCTC | 2 cut(s) | 873, 1249 |
| Alw44I | GTGCAC | 1 cut(s) | 1203 |
| Ama87I | CYCGRG | 1 cut(s) | 377 |
| Aor13HI | TCCGGA | 1 cut(s) | 871 |
| AoxI | GGCC | 1 cut(s) | 1145 |
| ApaLI | GTGCAC | 1 cut(s) | 1203 |
| ApeKI | GCWGC | 4 cut(s) | 546, 951, 1114, 1216 |
| ApoI | RAATTY | 2 cut(s) | 780, 823 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 513, 545 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 895, 1274 |
| AspS9I | GGNCC | 1 cut(s) | 395 |
| AsuII | TTCGAA | 1 cut(s) | 1268 |
| AsuNHI | GCTAGC | 1 cut(s) | 415 |
| AvaI | CYCGRG | 1 cut(s) | 377 |
| AvaII | GGWCC | 1 cut(s) | 395 |
| AxyI | CCTNAGG | 1 cut(s) | 460 |
| BaeGI | GKGCMC | 1 cut(s) | 1207 |
| BanI | GGYRCC | 1 cut(s) | 182 |
| Bbv12I | GWGCWC | 1 cut(s) | 1207 |
| BbvI | GCAGC | 4 cut(s) | 533, 963, 1101, 1228 |
| BccI | CCATC | 1 cut(s) | 708 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 29, 63 |
| BciVI | GTATCC | 1 cut(s) | 598 |
| BcoDI | GTCTC | 2 cut(s) | 873, 1249 |
| BfaI | CTAG | 4 cut(s) | 117, 416, 992, 1001 |
| BfmI | CTRYAG | 1 cut(s) | 916 |
| BfuAI | ACCTGC | 1 cut(s) | 70 |
| BfuI | GTATCC | 1 cut(s) | 598 |
| BglII | AGATCT | 1 cut(s) | 733 |
| BisI | GCNGC | 6 cut(s) | 57, 547, 952, 1065, 1115, 1217 |
| BlpI | GCTNAGC | 1 cut(s) | 216 |
| BlsI | GCNGC | 6 cut(s) | 58, 548, 953, 1066, 1116, 1218 |
| Bme18I | GGWCC | 1 cut(s) | 395 |
| BmeT110I | CYCGRG | 1 cut(s) | 377 |
| BmgT120I | GGNCC | 1 cut(s) | 395 |
| BmiI | GGNNCC | 2 cut(s) | 184, 1183 |
| BmtI | GCTAGC | 1 cut(s) | 419 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1212 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 216 |
| Bpu14I | TTCGAA | 1 cut(s) | 1268 |
| BsaJI | CCNNGG | 1 cut(s) | 882 |
| BsaWI | WCCGGW | 1 cut(s) | 871 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1011 |
| Bse1I | ACTGG | 2 cut(s) | 398, 453 |
| Bse21I | CCTNAGG | 1 cut(s) | 460 |
| BseAI | TCCGGA | 1 cut(s) | 871 |
| BseDI | CCNNGG | 1 cut(s) | 882 |
| BseGI | GGATG | 5 cut(s) | 213, 565, 766, 1165, 1249 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1011 |
| BseMII | CTCAG | 3 cut(s) | 300, 474, 851 |
| BseNI | ACTGG | 2 cut(s) | 398, 453 |
| BseSI | GKGCMC | 1 cut(s) | 1207 |
| BseXI | GCAGC | 4 cut(s) | 533, 963, 1101, 1228 |
| BseYI | CCCAGC | 1 cut(s) | 75 |
| BsgI | GTGCAG | 1 cut(s) | 223 |
| Bsh1236I | CGCG | 1 cut(s) | 856 |
| BshFI | GGCC | 1 cut(s) | 1147 |
| BshNI | GGYRCC | 1 cut(s) | 182 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1207 |
| BsiHKCI | CYCGRG | 1 cut(s) | 377 |
| BsiSI | CCGG | 2 cut(s) | 872, 1157 |
| BslFI | GGGAC | 2 cut(s) | 281, 829 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1011 |
| BsmAI | GTCTC | 2 cut(s) | 873, 1249 |
| BsmFI | GGGAC | 2 cut(s) | 281, 829 |
| BsmI | GAATGC | 1 cut(s) | 1276 |
| BsnI | GGCC | 1 cut(s) | 1147 |
| BsoBI | CYCGRG | 1 cut(s) | 377 |
| Bsp119I | TTCGAA | 1 cut(s) | 1268 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1207 |
| Bsp13I | TCCGGA | 1 cut(s) | 871 |
| Bsp1407I | TGTACA | 1 cut(s) | 1123 |
| Bsp143I | GATC | 5 cut(s) | 301, 318, 501, 733, 982 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 216 |
| Bsp19I | CCATGG | 1 cut(s) | 882 |
| BspACI | CCGC | 5 cut(s) | 17, 56, 848, 966, 1064 |
| BspANI | GGCC | 1 cut(s) | 1147 |
| BspCNI | CTCAG | 3 cut(s) | 301, 473, 850 |
| BspEI | TCCGGA | 1 cut(s) | 871 |
| BspFNI | CGCG | 1 cut(s) | 856 |
| BspLI | GGNNCC | 2 cut(s) | 184, 1183 |
| BspMI | ACCTGC | 1 cut(s) | 70 |
| BspOI | GCTAGC | 1 cut(s) | 419 |
| BspQI | GCTCTTC | 1 cut(s) | 625 |
| BspT104I | TTCGAA | 1 cut(s) | 1268 |
| BspT107I | GGYRCC | 1 cut(s) | 182 |
| BsrGI | TGTACA | 1 cut(s) | 1123 |
| BsrI | ACTGG | 2 cut(s) | 398, 453 |
| BssECI | CCNNGG | 1 cut(s) | 882 |
| BssMI | GATC | 5 cut(s) | 301, 318, 501, 733, 982 |
| BssT1I | CCWWGG | 1 cut(s) | 882 |
| Bst4CI | ACNGT | 4 cut(s) | 157, 920, 1192, 1243 |
| Bst6I | CTCTTC | 1 cut(s) | 625 |
| BstAUI | TGTACA | 1 cut(s) | 1123 |
| BstBI | TTCGAA | 1 cut(s) | 1268 |
| BstC8I | GCNNGC | 3 cut(s) | 417, 1062, 1154 |
| BstDEI | CTNAG | 5 cut(s) | 216, 309, 460, 837, 1212 |
| BstDSI | CCRYGG | 1 cut(s) | 882 |
| BstF5I | GGATG | 5 cut(s) | 213, 565, 766, 1165, 1249 |
| BstFNI | CGCG | 1 cut(s) | 856 |
| BstKTI | GATC | 5 cut(s) | 304, 321, 504, 736, 985 |
| BstMAI | GTCTC | 2 cut(s) | 873, 1249 |
| BstMBI | GATC | 5 cut(s) | 301, 318, 501, 733, 982 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 56, 1111, 1153 |
| BstNSI | RCATGY | 1 cut(s) | 1156 |
| BstSFI | CTRYAG | 1 cut(s) | 916 |
| BstSLI | GKGCMC | 1 cut(s) | 1207 |
| BstUI | CGCG | 1 cut(s) | 856 |
| BstV1I | GCAGC | 4 cut(s) | 533, 963, 1101, 1228 |
| BstX2I | RGATCY | 1 cut(s) | 733 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 405 |
| BstYI | RGATCY | 1 cut(s) | 733 |
| Bsu36I | CCTNAGG | 1 cut(s) | 460 |
| BsuI | GTATCC | 1 cut(s) | 598 |
| BsuRI | GGCC | 1 cut(s) | 1147 |
| BtgI | CCRYGG | 1 cut(s) | 882 |
| BtsCI | GGATG | 5 cut(s) | 213, 565, 766, 1165, 1249 |
| BtsI | GCAGTG | 1 cut(s) | 642 |
| BtsIMutI | CAGTG | 1 cut(s) | 642 |
| BveI | ACCTGC | 1 cut(s) | 70 |
| Cac8I | GCNNGC | 3 cut(s) | 417, 1062, 1154 |
| Cfr13I | GGNCC | 1 cut(s) | 395 |
| Csp6I | GTAC | 3 cut(s) | 456, 651, 1124 |
| CviAII | CATG | 6 cut(s) | 299, 538, 586, 883, 1011, 1153 |
| CviQI | GTAC | 3 cut(s) | 456, 651, 1124 |
| DdeI | CTNAG | 5 cut(s) | 216, 309, 460, 837, 1212 |
| DpnI | GATC | 5 cut(s) | 303, 320, 503, 735, 984 |
| DpnII | GATC | 5 cut(s) | 301, 318, 501, 733, 982 |
| Eam1104I | CTCTTC | 1 cut(s) | 625 |
| EarI | CTCTTC | 1 cut(s) | 625 |
| Eco130I | CCWWGG | 1 cut(s) | 882 |
| Eco47I | GGWCC | 1 cut(s) | 395 |
| Eco57I | CTGAAG | 1 cut(s) | 1068 |
| Eco81I | CCTNAGG | 1 cut(s) | 460 |
| Eco88I | CYCGRG | 1 cut(s) | 377 |
| EcoT14I | CCWWGG | 1 cut(s) | 882 |
| ErhI | CCWWGG | 1 cut(s) | 882 |
| FaeI | CATG | 6 cut(s) | 302, 541, 589, 886, 1014, 1156 |
| FaqI | GGGAC | 2 cut(s) | 281, 829 |
| FatI | CATG | 6 cut(s) | 298, 537, 585, 882, 1010, 1152 |
| FauI | CCCGC | 2 cut(s) | 10, 841 |
| FblI | GTMKAC | 1 cut(s) | 915 |
| Fnu4HI | GCNGC | 6 cut(s) | 57, 547, 952, 1065, 1115, 1217 |
| FokI | GGATG | 5 cut(s) | 220, 572, 773, 1172, 1236 |
| Fsp4HI | GCNGC | 6 cut(s) | 57, 547, 952, 1065, 1115, 1217 |
| FspBI | CTAG | 4 cut(s) | 117, 416, 992, 1001 |
| GluI | GCNGC | 6 cut(s) | 57, 547, 952, 1065, 1115, 1217 |
| GsaI | CCCAGC | 1 cut(s) | 79 |
| HaeIII | GGCC | 1 cut(s) | 1147 |
| HapII | CCGG | 2 cut(s) | 872, 1157 |
| Hin1II | CATG | 6 cut(s) | 302, 541, 589, 886, 1014, 1156 |
| HindIII | AAGCTT | 1 cut(s) | 598 |
| HinfI | GANTC | 6 cut(s) | 25, 65, 534, 620, 707, 1070 |
| HpaII | CCGG | 2 cut(s) | 872, 1157 |
| Hpy166II | GTNNAC | 5 cut(s) | 111, 653, 916, 1124, 1205 |
| Hpy188I | TCNGA | 7 cut(s) | 30, 310, 533, 787, 840, 910, 1087 |
| Hpy188III | TCNNGA | 4 cut(s) | 278, 484, 704, 872 |
| Hpy8I | GTNNAC | 5 cut(s) | 111, 653, 916, 1124, 1205 |
| HpyCH4III | ACNGT | 4 cut(s) | 157, 920, 1192, 1243 |
| HpyCH4IV | ACGT | 2 cut(s) | 655, 802 |
| HpyCH4V | TGCA | 5 cut(s) | 50, 204, 514, 1105, 1205 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 56, 1111, 1153 |
| HpyF3I | CTNAG | 5 cut(s) | 216, 309, 460, 837, 1212 |
| HpySE526I | ACGT | 2 cut(s) | 655, 802 |
| Hsp92II | CATG | 6 cut(s) | 302, 541, 589, 886, 1014, 1156 |
| Kpn2I | TCCGGA | 1 cut(s) | 871 |
| Kzo9I | GATC | 5 cut(s) | 301, 318, 501, 733, 982 |
| LguI | GCTCTTC | 1 cut(s) | 625 |
| Lsp1109I | GCAGC | 4 cut(s) | 533, 963, 1101, 1228 |
| MaeI | CTAG | 4 cut(s) | 117, 416, 992, 1001 |
| MaeII | ACGT | 2 cut(s) | 655, 802 |
| MaeIII | GTNAC | 3 cut(s) | 70, 312, 567 |
| MalI | GATC | 5 cut(s) | 303, 320, 503, 735, 984 |
| MboI | GATC | 5 cut(s) | 301, 318, 501, 733, 982 |
| MboII | GAAGA | 2 cut(s) | 642, 970 |
| MflI | RGATCY | 1 cut(s) | 733 |
| MhlI | GDGCHC | 1 cut(s) | 1207 |
| MluCI | AATT | 7 cut(s) | 236, 355, 780, 823, 1037, 1194, 1305 |
| MluI | ACGCGT | 1 cut(s) | 854 |
| MlyI | GAGTC | 2 cut(s) | 19, 74 |
| MnlI | CCTC | 7 cut(s) | 380, 469, 550, 677, 1230, 1263, 1289 |
| MroI | TCCGGA | 1 cut(s) | 871 |
| MroXI | GAANNNNTTC | 2 cut(s) | 895, 1274 |
| MseI | TTAA | 3 cut(s) | 239, 552, 660 |
| MspI | CCGG | 2 cut(s) | 872, 1157 |
| Mva1269I | GAATGC | 1 cut(s) | 1276 |
| MvnI | CGCG | 1 cut(s) | 856 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 56, 1111, 1153 |
| NcoI | CCATGG | 1 cut(s) | 882 |
| NdeII | GATC | 5 cut(s) | 301, 318, 501, 733, 982 |
| NheI | GCTAGC | 1 cut(s) | 415 |
| NlaIII | CATG | 6 cut(s) | 302, 541, 589, 886, 1014, 1156 |
| NlaIV | GGNNCC | 2 cut(s) | 184, 1183 |
| NmeAIII | GCCGAG | 1 cut(s) | 1173 |
| NspI | RCATGY | 1 cut(s) | 1156 |
| NspV | TTCGAA | 1 cut(s) | 1268 |
| PaeI | GCATGC | 1 cut(s) | 1156 |
| PciSI | GCTCTTC | 1 cut(s) | 625 |
| PctI | GAATGC | 1 cut(s) | 1276 |
| PdmI | GAANNNNTTC | 2 cut(s) | 895, 1274 |
| PfeI | GAWTC | 4 cut(s) | 534, 620, 707, 1070 |
| PkrI | GCNGC | 6 cut(s) | 58, 548, 953, 1066, 1116, 1218 |
| PleI | GAGTC | 2 cut(s) | 19, 73 |
| PpsI | GAGTC | 2 cut(s) | 19, 73 |
| PspFI | CCCAGC | 1 cut(s) | 75 |
| PspN4I | GGNNCC | 2 cut(s) | 184, 1183 |
| PspPI | GGNCC | 1 cut(s) | 395 |
| PsuI | RGATCY | 1 cut(s) | 733 |
| RsaI | GTAC | 3 cut(s) | 457, 652, 1125 |
| RsaNI | GTAC | 3 cut(s) | 456, 651, 1124 |
| SapI | GCTCTTC | 1 cut(s) | 625 |
| SaqAI | TTAA | 3 cut(s) | 239, 552, 660 |
| SatI | GCNGC | 6 cut(s) | 57, 547, 952, 1065, 1115, 1217 |
| Sau3AI | GATC | 5 cut(s) | 301, 318, 501, 733, 982 |
| Sau96I | GGNCC | 1 cut(s) | 395 |
| SchI | GAGTC | 2 cut(s) | 19, 74 |
| SduI | GDGCHC | 1 cut(s) | 1207 |
| SfcI | CTRYAG | 1 cut(s) | 916 |
| SfuI | TTCGAA | 1 cut(s) | 1268 |
| SinI | GGWCC | 1 cut(s) | 395 |
| SphI | GCATGC | 1 cut(s) | 1156 |
| Sse9I | AATT | 7 cut(s) | 236, 355, 780, 823, 1037, 1194, 1305 |
| SsiI | CCGC | 5 cut(s) | 17, 56, 848, 966, 1064 |
| SspI | AATATT | 2 cut(s) | 664, 894 |
| SspMI | CTAG | 4 cut(s) | 117, 416, 992, 1001 |
| StyI | CCWWGG | 1 cut(s) | 882 |
| TaaI | ACNGT | 4 cut(s) | 157, 920, 1192, 1243 |
| TaiI | ACGT | 2 cut(s) | 658, 805 |
| TaqI | TCGA | 4 cut(s) | 63, 618, 985, 1268 |
| TasI | AATT | 7 cut(s) | 236, 355, 780, 823, 1037, 1194, 1305 |
| TatI | WGTACW | 1 cut(s) | 1123 |
| TauI | GCSGC | 2 cut(s) | 59, 1067 |
| TfiI | GAWTC | 4 cut(s) | 534, 620, 707, 1070 |
| Tru1I | TTAA | 3 cut(s) | 239, 552, 660 |
| Tru9I | TTAA | 3 cut(s) | 239, 552, 660 |
| TscAI | CASTG | 1 cut(s) | 642 |
| TseI | GCWGC | 4 cut(s) | 546, 951, 1114, 1216 |
| TspDTI | ATGAA | 8 cut(s) | 130, 504, 574, 785, 1062, 1068, 1109, 1302 |
| TspGWI | ACGGA | 1 cut(s) | 365 |
| TspRI | CASTG | 1 cut(s) | 642 |
| VneI | GTGCAC | 1 cut(s) | 1203 |
| VpaK11BI | GGWCC | 1 cut(s) | 395 |
| XapI | RAATTY | 2 cut(s) | 780, 823 |
| XceI | RCATGY | 1 cut(s) | 1156 |
| XmiI | GTMKAC | 1 cut(s) | 915 |
| XmnI | GAANNNNTTC | 2 cut(s) | 895, 1274 |
| XspI | CTAG | 4 cut(s) | 117, 416, 992, 1001 |