RchiOBHm_Chr7g0212561

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000482
Physical Location & Seq
Reverse (-)
29793824 .. 29805063
11240 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19021

Sequence Viewer

Length: 2565 bp
ATGGAAGATGCCATTGATAAATTCAGGCTTTTCCATTCACACGATCACGGTCATGGATTCCGAGCTTCCCTTCATAAACTTTCTTGTATCATCAAGAAATGGAAAGCTAAGCATCAAATTTCTGCTGGTATTCAAAGAATAAACTCAAAAGTCAAAAACCTCTACGAGGGACATGAGAGATACAAACTTGTTGACCAAGCTGGCTCCAGTTCTGCTCGTCTGGTTCAGCGCCACAAGTTAGATCGAGGGGATGCCCTTTTACTGGAAGAGGCTGATCTTGTGGCAATTGGCGAGCGCAAAAAGCAACTGATTGAATTGCTTATGAAGGAAGACACTAGACGCCAGGTGGTTCCAGTAGTTGGGATGGGTGGACTGGGGAAAACTACCTTGGTCAAGCAAGTCTACGAGGATCCAAAAGTTCAGAAACGTTTCAAGGTACATGCTTGGATCACTGTTTCTCGATCATTCAAGATAAGTCAGCTCCTAAGACACATGATCAATAAAATCTTCAAAGTTATCAGGAAACCAAATAGTAGGTACCTGATTGTTCTGGATGATATATGGCATATACCCGACTGGGAGGCCATCAATCATGCAATGCCAAACAACAATCGTGGTAGTCGAGTAATGCTCACTACTCGCAATGTTAATGTAGCCTCTGCCTCTTGCTTGGGGAACCATGACATGTTCTACCGTTTAGAGCCCTTATCTCCAGAAGACTCATGGACTCTGTTCTGCAGGAAGACATTTCAAGGGAACACATGCCTTCCAAATTTGGAGGAAATTTGTCGATCCATCCTGATGAAATGTGGGGGACTGCCCCTTGCAATTGTGGCCATCAGCGCTGTACTAGCTTTAAAAGACAAGAGAAATTTGGATGATTGGGCTGCTGTTTGTGGCAGTATTGGAGCTGAAATAGAAGGAAATGATCAACTTGACAACATGAAAAAATTGCTGTATCTCAGTTACAGTGATTTACCATACCATTTGAAGTCTTGTTTCTTGTATCTGAGCATCTTTCCTGATCTCTACAAAATTGAGCATATGAGATTGATTCGGTTATGGTTAGCTGAAGGATTTGTTATTGGAAAAGAGGGAAGGACACCAGAAGAAGTTGCAGAGAGTTACCTTAGAGAGTTATTGGACAGAAGTTTGATTCAAGCAGCAGAAACATCAACTGATGGGAGAGTTAAATCATATCGCATCCATGATCTTTTACGGGAGATTGTCATTTTGAAGTCTAGAGAGCAAAATTTTGCAGCAATAGAAAAAGAGCACGGTACAATGTGGTCTGAGAAAGTTCGACGACTATCAATAGTTAACACACTGCAGAATGTACATCAAAAGAGGACACCGTCTCAACTTCGTTCTTTGCTCGTTTTTGGGGTAGAAGATTCACTTACTGATTTTTCCATACCAAAATTGTTTCCTACAGGTGTTCAGCTGCTTACAGTATTAGACTTACAAGGTGCACATCTGGATATGTTTCCAAGAGTGGTTGTCAAGCTTCTTCTTCTCAGGTATCTTAGCTTGAGGCATACAAAGGTGAAACAAATTCCAGGCTCCATCAAGAAGCTACAATACCTGGAGACCTTGGATCTTAAACACTCACAAGTTGTTGAATTACCTTCTGAGATTTTGAATCTCAAGCGACTTCGTCATCTCCTGGTGTATCGTTATGAGGTTGAGTCATACGCACGCTTCAATGCCAGAAAAGGAGTTAAGGTCCCTGCAAGAATATGTGGCTTGCAATTGCTCCAAAAGCTCTGCTTTATAGAGGCAAATGAAGACAATGGTGCTTTAATGGCAGAGCTCGGGAAAATGAACCAACTAAGGAGGTTAGGCATTTTCAAGTTGAAAAACGAAGATGGGGTCACCTTATGCTCGTCCATCCAAAATATGACCAATCTTCGCTCGTTCTCTGTATCTTCAACAGAGAAGGACAAGATAATTGATCTTAGCCACATTTCTCGTGCTCCTCCATTTCTTCAGCGACTCTACTTGACAGGTCGTTTAGAAAAGTTACCACACTGGATTTCTTCTCTTCAAAACCTGGTCAGACTGTTTTTAAAATGGAGTCGGTTGAAGGAGGATCCTCTATTACATCTTCAGGGTTTGCCAAACCTGGTACACCTTGAACTGCTACAAGTTTATGATGGAGGAAGCTTGCGTTTTGAGGCAGGAGGGTTTCCAAGTCTGAAGTTATTAGGTATTGATAAATTGGATGAGCTACAATCAGTAACCATAGAGGAGGGAGCAATGCCTTGTCTAGAAAAGCTAATTATCCAGCGGTGTAAATTGTTGAAGAAGGTCCCATCTGGCATTGAACATTTAACCAATTTAAAGCTGCTTGAATTTTTCGATATGCCAGATGAATTAATCATGCCACTACATCCAGATGGTGGAGAAGATCATTGGAAAGTTGCTCATGTTCCAGCAGTTTATTATTCCTATTGGAGAGGTGGGGGTTGGGACGTCTATTCATTAGTCAATGAAGGGGAGAGTTCTAATTCGGGTACTTCTGCTGTGAGGAGGCTTGAACGTAGTATTCTGTGGAAGGTGTAG

Protein Analysis

854

Amino Acids

97.86

Weight (kDa)

9.32

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 99 - 169 1.3e-17 NB-ARC domain
AAA_14 PF13173 114 - 213 4.3e-06 AAA domain
NB-ARC PF00931 167 - 253 3.9e-19 NB-ARC domain
WHD_DRP PF23559 339 - 410 9.3e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 454 - 784 9.9e-47 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000482)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g23450 FvH4_5g23460 FvH4_5g23470 FvH4_5g24290 FvH4_5g24300
malus_domestica MD06G1024000.v1.1
prunus_persica Prupe.5G025000_v2.0.a1 Prupe.5G025000_v2.0.a1 Prupe.5G025100_v2.0.a1 Prupe.5G025200_v2.0.a1
pyrus_communis pycom16g25280
rosa_chinensis RchiOBHm_Chr7g0212511 RchiOBHm_Chr7g0212531 RchiOBHm_Chr7g0212541 RchiOBHm_Chr7g0212561 RchiOBHm_Chr7g0212571 RchiOBHm_Chr7g0212621 RchiOBHm_Chr7g0212631 RchiOBHm_Chr7g0212671 RchiOBHm_Chr7g0212691 RchiOBHm_Chr7g0212701
rosa_laevigata RLG00000002879 RLG00000002883 RLG00000002884 RLG00000002885
rosa_multiflora Rmu_co8121580.1_g000001 Rmu_sc0000394.1_g000014 Rmu_sc0000394.1_g000015 Rmu_sc0001435.1_g000001 Rmu_sc0005246.1_g000005 Rmu_sc0005246.1_g000010 Rmu_sc0005246.1_g000015 Rmu_sc0005246.1_g000016 Rmu_sc0005770.1_g000018 Rmu_sc0005770.1_g000019 Rmu_sc0005770.1_g000020 Rmu_sc0007576.1_g000019 Rmu_sc0009400.1_g000001 Rmu_sc0011082.1_g000001 Rmu_sc0038076.1_g000002
rosa_roxburghii Rroxscaffold_3G00246720
rosa_rugosa Rorug07G0134800 Rorug07G0134900 Rorug07G0135100 Rorug07G0135200 Rorug07G0135400 Rorug07G0135500 Rorug07G0135800 Rorug07G0135900
rosa_samantha Rh2AG310100 Rh2CG296700 Rh2CG297000 Rh2CG301700 Rh7BG258900 Rh7BG261600 Rh7BG261700 Rh7BG261800 Rh7BG261900 Rh7BG262000 Rh7BG262100 Rh7BG262400 Rh7BG262500 Rh7BG263100 Rh7BG263200 Rh7CG284400 Rh7CG284600 Rh7CG284700 Rh7CG284900 Rh7CG285100 Rh7CG285500 Rh7CG285600 Rh7CG285700 Rh7CG286200 Rh7CG286600 Rh7CG286700 Rh7DG274400 Rh7DG274600 Rh7DG274800 Rh7DG274900 Rh7DG275100 Rh7DG275200 Rh7DG275500 Rh7DG275600 Rh7DG275700
rosa_wichuraiana Rw7G022910 Rw7G022960 Rw7G022980 Rw7G023010 Rw7G023050 Rw7G023110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2478
Acc65I GGTACC 1 cut(s) 537
AccB1I GGYRCC 1 cut(s) 537
AccB7I CCANNNNNTGG 2 cut(s) 359, 2402
AccI GTMKAC 1 cut(s) 402
AciI CCGC 1 cut(s) 2290
AclI AACGTT 1 cut(s) 427
AclWI GGATC 7 cut(s) 404, 417, 455, 786, 1605, 2087, 2100
AcoI YGGCCR 1 cut(s) 834
AcsI RAATTY 8 cut(s) 20, 117, 772, 783, 871, 1252, 1554, 2354
AcuI CTGAAG 4 cut(s) 1092, 1973, 2093, 2219
AcyI GRCGYC 2 cut(s) 340, 2475
AfaI GTAC 7 cut(s) 438, 539, 849, 1282, 1338, 2130, 2518
AfeI AGCGCT 1 cut(s) 844
AfiI CCNNNNNNNGG 4 cut(s) 166, 262, 359, 2402
AflIII ACRYGT 1 cut(s) 684
AjnI CCWGG 6 cut(s) 342, 1558, 1584, 1665, 2052, 2124
AjuI GAANNNNNNNTTGG 2 cut(s) 371, 403
Alw21I GWGCWC 4 cut(s) 1278, 1474, 1815, 1978
Alw26I GTCTC 2 cut(s) 1362, 1583
Alw44I GTGCAC 1 cut(s) 1470
AlwI GGATC 7 cut(s) 404, 417, 455, 786, 1605, 2087, 2100
Ama87I CYCGRG 1 cut(s) 1814
Aor51HI AGCGCT 1 cut(s) 844
AoxI GGCC 2 cut(s) 582, 834
ApaLI GTGCAC 1 cut(s) 1470
ApeKI GCWGC 5 cut(s) 887, 1163, 1259, 1444, 2347
ApoI RAATTY 8 cut(s) 20, 117, 772, 783, 871, 1252, 1554, 2354
ArsI GACNNNNNNTTYG 2 cut(s) 1857, 1889
AseI ATTAAT 1 cut(s) 2378
Asp700I GAANNNNTTC 1 cut(s) 428
Asp718I GGTACC 1 cut(s) 537
AspLEI GCGC 3 cut(s) 231, 297, 845
AspS9I GGNCC 2 cut(s) 1726, 2311
AsuHPI GGTGA 2 cut(s) 1558, 1867
AvaI CYCGRG 1 cut(s) 1814
AvaII GGWCC 2 cut(s) 1726, 2311
BaeGI GKGCMC 1 cut(s) 1474
BalI TGGCCA 1 cut(s) 836
BamHI GGATCC 2 cut(s) 409, 2092
BanI GGYRCC 1 cut(s) 537
BanII GRGCYC 2 cut(s) 705, 1815
BarI GAAGNNNNNNTAC 2 cut(s) 1565, 1597
BauI CACGAG 1 cut(s) 1971
BbsI GAAGAC 4 cut(s) 336, 723, 749, 1794
Bbv12I GWGCWC 4 cut(s) 1278, 1474, 1815, 1978
BbvI GCAGC 5 cut(s) 874, 1175, 1271, 1431, 2334
BciT130I CCWGG 6 cut(s) 344, 1560, 1586, 1667, 2054, 2126
BclI TGATCA 2 cut(s) 495, 928
BcoDI GTCTC 2 cut(s) 1362, 1583
BfaI CTAG 4 cut(s) 336, 851, 1242, 2270
BfmI CTRYAG 3 cut(s) 736, 1328, 1431
BfoI RGCGCY 2 cut(s) 232, 846
BisI GCNGC 5 cut(s) 888, 1164, 1260, 1445, 2348
BlpI GCTNAGC 1 cut(s) 108
BlsI GCNGC 5 cut(s) 889, 1165, 1261, 1446, 2349
Bme1390I CCNGG 6 cut(s) 344, 1560, 1586, 1667, 2054, 2126
Bme18I GGWCC 2 cut(s) 1726, 2311
BmeT110I CYCGRG 1 cut(s) 1814
BmgT120I GGNCC 2 cut(s) 1726, 2311
BmiI GGNNCC 9 cut(s) 205, 351, 411, 539, 677, 1564, 1728, 2094, 2313
BmrFI CCNGG 6 cut(s) 344, 1560, 1586, 1667, 2054, 2126
BmrI ACTGGG 2 cut(s) 383, 586
BmsI GCATC 4 cut(s) 121, 241, 1023, 1212
BmuI ACTGGG 2 cut(s) 383, 586
BpiI GAAGAC 4 cut(s) 336, 723, 749, 1794
BplI GAGNNNNNCTC 2 cut(s) 615, 647
BpmI CTGGAG 3 cut(s) 190, 696, 1607
Bpu1102I GCTNAGC 1 cut(s) 108
BpuEI CTTGAG 2 cut(s) 1552, 1631
BsaHI GRCGYC 2 cut(s) 340, 2475
BsaI GGTCTC 1 cut(s) 1583
BsaJI CCNNGG 2 cut(s) 387, 1593
BsaXI ACNNNNNCTCC 2 cut(s) 1710, 1740
Bsc4I CCNNNNNNNGG 4 cut(s) 166, 262, 359, 2402
Bse1I ACTGG 6 cut(s) 207, 267, 353, 378, 581, 2036
Bse3DI GCAATG 3 cut(s) 603, 649, 2265
BseBI CCWGG 6 cut(s) 344, 1560, 1586, 1667, 2054, 2126
BseDI CCNNGG 2 cut(s) 387, 1593
BseGI GGATG 9 cut(s) 256, 369, 559, 795, 883, 1203, 1890, 2230, 2392
BseLI CCNNNNNNNGG 4 cut(s) 166, 262, 359, 2402
BseMI GCAATG 3 cut(s) 603, 649, 2265
BseMII CTCAG 5 cut(s) 976, 1001, 1284, 1531, 1623
BseNI ACTGG 6 cut(s) 207, 267, 353, 378, 581, 2036
BseRI GAGGAG 3 cut(s) 1968, 2264, 2545
BseSI GKGCMC 1 cut(s) 1474
BseXI GCAGC 5 cut(s) 874, 1175, 1271, 1431, 2334
BshFI GGCC 2 cut(s) 584, 836
BshNI GGYRCC 1 cut(s) 537
BsiHKAI GWGCWC 4 cut(s) 1278, 1474, 1815, 1978
BsiHKCI CYCGRG 1 cut(s) 1814
BslFI GGGAC 5 cut(s) 183, 828, 1712, 2297, 2486
BslI CCNNNNNNNGG 4 cut(s) 166, 262, 359, 2402
BsmAI GTCTC 2 cut(s) 1362, 1583
BsmBI CGTCTC 1 cut(s) 1362
BsmFI GGGAC 5 cut(s) 183, 828, 1712, 2297, 2486
BsnI GGCC 2 cut(s) 584, 836
Bso31I GGTCTC 1 cut(s) 1583
BsoBI CYCGRG 1 cut(s) 1814
Bsp1286I GDGCHC 5 cut(s) 705, 1278, 1474, 1815, 1978
Bsp1407I TGTACA 1 cut(s) 1336
Bsp1720I GCTNAGC 1 cut(s) 108
BspACI CCGC 1 cut(s) 2290
BspANI GGCC 2 cut(s) 584, 836
BspCNI CTCAG 5 cut(s) 975, 1002, 1285, 1530, 1624
BspLI GGNNCC 9 cut(s) 205, 351, 411, 539, 677, 1564, 1728, 2094, 2313
BspMAI CTGCAG 2 cut(s) 740, 1332
BspPI GGATC 7 cut(s) 404, 417, 455, 786, 1605, 2087, 2100
BspT107I GGYRCC 1 cut(s) 537
BspTNI GGTCTC 1 cut(s) 1583
BsrDI GCAATG 3 cut(s) 603, 649, 2265
BsrGI TGTACA 1 cut(s) 1336
BsrI ACTGG 6 cut(s) 207, 267, 353, 378, 581, 2036
BssECI CCNNGG 2 cut(s) 387, 1593
BssNI GRCGYC 2 cut(s) 340, 2475
BssSI CACGAG 1 cut(s) 1971
BssT1I CCWWGG 2 cut(s) 387, 1593
Bst2BI CACGAG 1 cut(s) 1971
Bst2UI CCWGG 6 cut(s) 344, 1560, 1586, 1667, 2054, 2126
Bst4CI ACNGT 8 cut(s) 50, 454, 695, 971, 1280, 1356, 1453, 2064
Bst6I CTCTTC 2 cut(s) 261, 2049
BstACI GRCGYC 2 cut(s) 340, 2475
BstAUI TGTACA 1 cut(s) 1336
BstC8I GCNNGC 5 cut(s) 202, 293, 1699, 1748, 2168
BstEII GGTNACC 1 cut(s) 1873
BstENI CCTNNNNNAGG 1 cut(s) 164
BstF5I GGATG 9 cut(s) 256, 369, 559, 795, 883, 1203, 1890, 2230, 2392
BstH2I RGCGCY 2 cut(s) 232, 846
BstHHI GCGC 3 cut(s) 231, 297, 845
BstMAI GTCTC 2 cut(s) 1362, 1583
BstMWI GCNNNNNNNGC 6 cut(s) 301, 833, 842, 851, 1762, 1805
BstNI CCWGG 6 cut(s) 344, 1560, 1586, 1667, 2054, 2126
BstNSI RCATGY 3 cut(s) 443, 688, 765
BstPI GGTNACC 1 cut(s) 1873
BstSCI CCNGG 6 cut(s) 342, 1558, 1584, 1665, 2052, 2124
BstSFI CTRYAG 3 cut(s) 736, 1328, 1431
BstSLI GKGCMC 1 cut(s) 1474
BstV1I GCAGC 5 cut(s) 874, 1175, 1271, 1431, 2334
BstV2I GAAGAC 4 cut(s) 336, 723, 749, 1794
BstX2I RGATCY 3 cut(s) 409, 1597, 2092
BstYI RGATCY 3 cut(s) 409, 1597, 2092
BsuRI GGCC 2 cut(s) 584, 836
BtsCI GGATG 9 cut(s) 256, 369, 559, 795, 883, 1203, 1890, 2230, 2392
BtsI GCAGTG 1 cut(s) 1325
BtsIMutI CAGTG 4 cut(s) 450, 976, 1325, 2029
Cac8I GCNNGC 5 cut(s) 202, 293, 1699, 1748, 2168
CfoI GCGC 3 cut(s) 231, 297, 845
Cfr13I GGNCC 2 cut(s) 1726, 2311
CseI GACGC 1 cut(s) 348
CsiI ACCWGGT 2 cut(s) 2052, 2124
Csp6I GTAC 7 cut(s) 437, 538, 848, 1281, 1337, 2129, 2517
CspCI CAANNNNNGTGG 2 cut(s) 595, 630
CviQI GTAC 7 cut(s) 437, 538, 848, 1281, 1337, 2129, 2517
DraI TTTAAA 3 cut(s) 858, 2070, 2343
EaeI YGGCCR 1 cut(s) 834
Eam1104I CTCTTC 2 cut(s) 261, 2049
EarI CTCTTC 2 cut(s) 261, 2049
Ecl136II GAGCTC 1 cut(s) 1813
Eco130I CCWWGG 2 cut(s) 387, 1593
Eco24I GRGCYC 2 cut(s) 705, 1815
Eco31I GGTCTC 1 cut(s) 1583
Eco47I GGWCC 2 cut(s) 1726, 2311
Eco47III AGCGCT 1 cut(s) 844
Eco53kI GAGCTC 1 cut(s) 1813
Eco57I CTGAAG 4 cut(s) 1092, 1973, 2093, 2219
Eco88I CYCGRG 1 cut(s) 1814
Eco91I GGTNACC 1 cut(s) 1873
EcoICRI GAGCTC 1 cut(s) 1813
EcoNI CCTNNNNNAGG 1 cut(s) 164
EcoO109I RGGNCCY 2 cut(s) 1726, 2311
EcoO65I GGTNACC 1 cut(s) 1873
EcoRII CCWGG 6 cut(s) 342, 1558, 1584, 1665, 2052, 2124
EcoT14I CCWWGG 2 cut(s) 387, 1593
EcoT38I GRGCYC 2 cut(s) 705, 1815
ErhI CCWWGG 2 cut(s) 387, 1593
Esp3I CGTCTC 1 cut(s) 1362
FalI AAGNNNNNCTT 4 cut(s) 1383, 1415, 1754, 1786
FaqI GGGAC 5 cut(s) 183, 828, 1712, 2297, 2486
FauNDI CATATG 1 cut(s) 1044
FbaI TGATCA 2 cut(s) 495, 928
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 5 cut(s) 888, 1164, 1260, 1445, 2348
FokI GGATG 9 cut(s) 263, 376, 566, 782, 890, 1190, 1877, 2237, 2379
FriOI GRGCYC 2 cut(s) 705, 1815
Fsp4HI GCNGC 5 cut(s) 888, 1164, 1260, 1445, 2348
FspBI CTAG 4 cut(s) 336, 851, 1242, 2270
GlaI GCGC 3 cut(s) 230, 296, 844
GluI GCNGC 5 cut(s) 888, 1164, 1260, 1445, 2348
GsuI CTGGAG 3 cut(s) 190, 696, 1607
HaeII RGCGCY 2 cut(s) 232, 846
HaeIII GGCC 2 cut(s) 584, 836
HgaI GACGC 1 cut(s) 348
HhaI GCGC 3 cut(s) 231, 297, 845
Hin1I GRCGYC 2 cut(s) 340, 2475
Hin6I GCGC 3 cut(s) 229, 295, 843
HinP1I GCGC 3 cut(s) 229, 295, 843
HincII GTYRAC 2 cut(s) 193, 1321
HindII GTYRAC 2 cut(s) 193, 1321
HindIII AAGCTT 2 cut(s) 1505, 2164
HpaI GTTAAC 1 cut(s) 1321
HphI GGTGA 2 cut(s) 1558, 1867
Hpy166II GTNNAC 6 cut(s) 193, 371, 403, 1321, 1472, 2131
Hpy188I TCNGA 7 cut(s) 62, 423, 1011, 1294, 1633, 2060, 2199
Hpy8I GTNNAC 6 cut(s) 193, 371, 403, 1321, 1472, 2131
Hpy99I CGWCG 1 cut(s) 1308
HpyCH4III ACNGT 8 cut(s) 50, 454, 695, 971, 1280, 1356, 1453, 2064
HpyCH4IV ACGT 3 cut(s) 427, 2475, 2542
HpyCH4V TGCA 9 cut(s) 596, 738, 827, 1118, 1259, 1330, 1472, 1733, 1750
HpyF10VI GCNNNNNNNGC 6 cut(s) 301, 833, 842, 851, 1762, 1805
HpySE526I ACGT 3 cut(s) 427, 2475, 2542
Hsp92I GRCGYC 2 cut(s) 340, 2475
HspAI GCGC 3 cut(s) 229, 295, 843
KpnI GGTACC 1 cut(s) 541
Ksp22I TGATCA 2 cut(s) 495, 928
KspAI GTTAAC 1 cut(s) 1321
LmnI GCTCC 7 cut(s) 209, 486, 908, 1568, 1761, 1981, 2255
Lsp1109I GCAGC 5 cut(s) 874, 1175, 1271, 1431, 2334
LweI GCATC 4 cut(s) 121, 241, 1023, 1212
MabI ACCWGGT 2 cut(s) 2052, 2124
MaeI CTAG 4 cut(s) 336, 851, 1242, 2270
MaeII ACGT 3 cut(s) 427, 2475, 2542
MaeIII GTNAC 5 cut(s) 965, 1124, 1873, 2022, 2239
MfeI CAATTG 3 cut(s) 285, 828, 1751
MflI RGATCY 3 cut(s) 409, 1597, 2092
MhlI GDGCHC 5 cut(s) 705, 1278, 1474, 1815, 1978
MlsI TGGCCA 1 cut(s) 836
MluNI TGGCCA 1 cut(s) 836
MlyI GAGTC 5 cut(s) 713, 721, 1697, 1989, 2086
Mox20I TGGCCA 1 cut(s) 836
MroXI GAANNNNTTC 1 cut(s) 428
MscI TGGCCA 1 cut(s) 836
MslI CAYNNNNRTG 3 cut(s) 51, 800, 2397
Msp20I TGGCCA 1 cut(s) 836
MspA1I CMGCKG 2 cut(s) 1444, 2290
MspR9I CCNGG 6 cut(s) 344, 1560, 1586, 1667, 2054, 2126
MunI CAATTG 3 cut(s) 285, 828, 1751
MvaI CCWGG 6 cut(s) 344, 1560, 1586, 1667, 2054, 2126
MwoI GCNNNNNNNGC 6 cut(s) 301, 833, 842, 851, 1762, 1805
NdeI CATATG 1 cut(s) 1044
NlaIV GGNNCC 9 cut(s) 205, 351, 411, 539, 677, 1564, 1728, 2094, 2313
NmuCI GTSAC 1 cut(s) 1873
NspI RCATGY 3 cut(s) 443, 688, 765
PciI ACATGT 1 cut(s) 684
PcsI WCGNNNNNNNCGW 1 cut(s) 619
PdmI GAANNNNTTC 1 cut(s) 428
PfeI GAWTC 5 cut(s) 57, 1054, 1156, 1394, 1642
PflFI GACNNNGTC 2 cut(s) 1354, 1656
PflMI CCANNNNNTGG 2 cut(s) 359, 2402
PkrI GCNGC 5 cut(s) 889, 1165, 1261, 1446, 2349
PleI GAGTC 5 cut(s) 713, 721, 1696, 1989, 2085
PpsI GAGTC 5 cut(s) 713, 721, 1696, 1989, 2085
PpuMI RGGWCCY 2 cut(s) 1726, 2311
PscI ACATGT 1 cut(s) 684
PshBI ATTAAT 1 cut(s) 2378
Psp124BI GAGCTC 1 cut(s) 1815
Psp1406I AACGTT 1 cut(s) 427
Psp5II RGGWCCY 2 cut(s) 1726, 2311
Psp6I CCWGG 6 cut(s) 342, 1558, 1584, 1665, 2052, 2124
PspEI GGTNACC 1 cut(s) 1873
PspGI CCWGG 6 cut(s) 342, 1558, 1584, 1665, 2052, 2124
PspN4I GGNNCC 9 cut(s) 205, 351, 411, 539, 677, 1564, 1728, 2094, 2313
PspPI GGNCC 2 cut(s) 1726, 2311
PspPPI RGGWCCY 2 cut(s) 1726, 2311
PstI CTGCAG 2 cut(s) 740, 1332
PsuI RGATCY 3 cut(s) 409, 1597, 2092
PsyI GACNNNGTC 2 cut(s) 1354, 1656
PvuII CAGCTG 1 cut(s) 1444
RsaI GTAC 7 cut(s) 438, 539, 849, 1282, 1338, 2130, 2518
RsaNI GTAC 7 cut(s) 437, 538, 848, 1281, 1337, 2129, 2517
RseI CAYNNNNRTG 3 cut(s) 51, 800, 2397
SacI GAGCTC 1 cut(s) 1815
SatI GCNGC 5 cut(s) 888, 1164, 1260, 1445, 2348
Sau96I GGNCC 2 cut(s) 1726, 2311
SchI GAGTC 5 cut(s) 713, 721, 1697, 1989, 2086
ScrFI CCNGG 6 cut(s) 344, 1560, 1586, 1667, 2054, 2126
SduI GDGCHC 5 cut(s) 705, 1278, 1474, 1815, 1978
SexAI ACCWGGT 2 cut(s) 2052, 2124
SfaNI GCATC 4 cut(s) 121, 241, 1023, 1212
SfcI CTRYAG 3 cut(s) 736, 1328, 1431
SinI GGWCC 2 cut(s) 1726, 2311
SmiMI CAYNNNNRTG 3 cut(s) 51, 800, 2397
SmlI CTYRAG 2 cut(s) 1531, 1646
SmoI CTYRAG 2 cut(s) 1531, 1646
SsiI CCGC 1 cut(s) 2290
SspMI CTAG 4 cut(s) 336, 851, 1242, 2270
SstI GAGCTC 1 cut(s) 1815
StyD4I CCNGG 6 cut(s) 342, 1558, 1584, 1665, 2052, 2124
StyI CCWWGG 2 cut(s) 387, 1593
TaaI ACNGT 8 cut(s) 50, 454, 695, 971, 1280, 1356, 1453, 2064
TaiI ACGT 3 cut(s) 430, 2478, 2545
TaqI TCGA 6 cut(s) 244, 460, 622, 790, 1303, 2361
TatI WGTACW 2 cut(s) 847, 1336
TfiI GAWTC 5 cut(s) 57, 1054, 1156, 1394, 1642
TscAI CASTG 4 cut(s) 457, 976, 1332, 2036
TseFI GTSAC 1 cut(s) 1873
TseI GCWGC 5 cut(s) 887, 1163, 1259, 1444, 2347
Tsp45I GTSAC 1 cut(s) 1873
TspDTI ATGAA 9 cut(s) 62, 338, 818, 959, 1800, 1838, 2388, 2472, 2508
TspRI CASTG 4 cut(s) 457, 976, 1332, 2036
Tth111I GACNNNGTC 2 cut(s) 1354, 1656
Van91I CCANNNNNTGG 2 cut(s) 359, 2402
VneI GTGCAC 1 cut(s) 1470
VpaK11BI GGWCC 2 cut(s) 1726, 2311
VspI ATTAAT 1 cut(s) 2378
XagI CCTNNNNNAGG 1 cut(s) 164
XapI RAATTY 8 cut(s) 20, 117, 772, 783, 871, 1252, 1554, 2354
XbaI TCTAGA 2 cut(s) 1241, 2269
XceI RCATGY 3 cut(s) 443, 688, 765
XcmI CCANNNNNNNNNTGG 1 cut(s) 720
XmiI GTMKAC 1 cut(s) 402
XmnI GAANNNNTTC 1 cut(s) 428
XspI CTAG 4 cut(s) 336, 851, 1242, 2270
ZraI GACGTC 1 cut(s) 2476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.