Belongs to the disease resistance NB-LRR family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1063, 1799 |
| AccI | GTMKAC | 1 cut(s) | 1875 |
| AccII | CGCG | 1 cut(s) | 1311 |
| AciI | CCGC | 1 cut(s) | 1863 |
| AclWI | GGATC | 3 cut(s) | 73, 86, 1484 |
| AcoI | YGGCCR | 1 cut(s) | 686 |
| AcsI | RAATTY | 7 cut(s) | 233, 649, 951, 1379, 1519, 1724, 1751 |
| AcuI | CTGAAG | 4 cut(s) | 821, 1311, 1370, 1616 |
| AcyI | GRCGYC | 1 cut(s) | 553 |
| AfaI | GTAC | 5 cut(s) | 277, 735, 1397, 1527, 1915 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 88, 266, 475, 1063, 1799, 1929 |
| AflII | CTTAAG | 1 cut(s) | 284 |
| AjiI | CACGTC | 1 cut(s) | 386 |
| AjnI | CCWGG | 5 cut(s) | 225, 547, 555, 1062, 1449 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1107, 1139 |
| Alw21I | GWGCWC | 2 cut(s) | 871, 1375 |
| Alw26I | GTCTC | 1 cut(s) | 376 |
| Alw44I | GTGCAC | 1 cut(s) | 867 |
| AlwI | GGATC | 3 cut(s) | 73, 86, 1484 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 575, 991, 1393 |
| AoxI | GGCC | 1 cut(s) | 686 |
| ApaLI | GTGCAC | 1 cut(s) | 867 |
| ApeKI | GCWGC | 4 cut(s) | 335, 482, 560, 656 |
| ApoI | RAATTY | 7 cut(s) | 233, 649, 951, 1379, 1519, 1724, 1751 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 168, 200 |
| AseI | ATTAAT | 1 cut(s) | 1775 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 696, 1383, 1626 |
| AspLEI | GCGC | 2 cut(s) | 120, 1313 |
| AspS9I | GGNCC | 3 cut(s) | 994, 1453, 1708 |
| AsuC2I | CCSGG | 1 cut(s) | 1832 |
| AsuHPI | GGTGA | 3 cut(s) | 73, 173, 955 |
| AsuII | TTCGAA | 1 cut(s) | 700 |
| AvaII | GGWCC | 3 cut(s) | 994, 1453, 1708 |
| AxyI | CCTNAGG | 1 cut(s) | 929 |
| BaeGI | GKGCMC | 1 cut(s) | 871 |
| BalI | TGGCCA | 1 cut(s) | 688 |
| BamHI | GGATCC | 1 cut(s) | 78 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 962, 994 |
| BbsI | GAAGAC | 3 cut(s) | 162, 709, 827 |
| Bbv12I | GWGCWC | 2 cut(s) | 871, 1375 |
| BbvI | GCAGC | 4 cut(s) | 322, 494, 572, 668 |
| BccI | CCATC | 9 cut(s) | 208, 572, 760, 971, 1065, 1259, 1509, 1547, 1793 |
| BciT130I | CCWGG | 5 cut(s) | 227, 549, 557, 1064, 1451 |
| BciVI | GTATCC | 1 cut(s) | 611 |
| BcnI | CCSGG | 1 cut(s) | 1832 |
| BcoDI | GTCTC | 1 cut(s) | 376 |
| BfaI | CTAG | 4 cut(s) | 1271, 1604, 1667, 1960 |
| BfmI | CTRYAG | 2 cut(s) | 828, 1128 |
| BfrI | CTTAAG | 1 cut(s) | 284 |
| BfuI | GTATCC | 1 cut(s) | 611 |
| BisI | GCNGC | 4 cut(s) | 336, 483, 561, 657 |
| BlsI | GCNGC | 4 cut(s) | 337, 484, 562, 658 |
| Bme1390I | CCNGG | 6 cut(s) | 227, 549, 557, 1064, 1451, 1832 |
| Bme18I | GGWCC | 3 cut(s) | 994, 1453, 1708 |
| BmgBI | CACGTC | 1 cut(s) | 386 |
| BmgT120I | GGNCC | 3 cut(s) | 994, 1453, 1708 |
| BmiI | GGNNCC | 5 cut(s) | 80, 418, 507, 1125, 1710 |
| BmrFI | CCNGG | 6 cut(s) | 227, 549, 557, 1064, 1451, 1832 |
| BmsI | GCATC | 4 cut(s) | 136, 211, 309, 454 |
| BpiI | GAAGAC | 3 cut(s) | 162, 709, 827 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1807, 1839 |
| Bpu14I | TTCGAA | 1 cut(s) | 700 |
| BpuEI | CTTGAG | 1 cut(s) | 1028 |
| BpuMI | CCSGG | 1 cut(s) | 1832 |
| BsaHI | GRCGYC | 1 cut(s) | 553 |
| BsaJI | CCNNGG | 1 cut(s) | 225 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1107, 1137 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 88, 266, 475, 1063, 1799, 1929 |
| Bse1I | ACTGG | 4 cut(s) | 54, 480, 1433, 1734 |
| Bse21I | CCTNAGG | 1 cut(s) | 929 |
| Bse3DI | GCAATG | 2 cut(s) | 666, 1662 |
| BseBI | CCWGG | 5 cut(s) | 227, 549, 557, 1064, 1451 |
| BseDI | CCNNGG | 1 cut(s) | 225 |
| BseGI | GGATG | 3 cut(s) | 469, 1789, 1876 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 88, 266, 475, 1063, 1799, 1929 |
| BseMI | GCAATG | 2 cut(s) | 666, 1662 |
| BseMII | CTCAG | 5 cut(s) | 388, 920, 928, 1020, 1346 |
| BseNI | ACTGG | 4 cut(s) | 54, 480, 1433, 1734 |
| BseRI | GAGGAG | 1 cut(s) | 946 |
| BseSI | GKGCMC | 1 cut(s) | 871 |
| BseXI | GCAGC | 4 cut(s) | 322, 494, 572, 668 |
| BsgI | GTGCAG | 1 cut(s) | 746 |
| Bsh1236I | CGCG | 1 cut(s) | 1311 |
| BshFI | GGCC | 1 cut(s) | 688 |
| BsiHKAI | GWGCWC | 2 cut(s) | 871, 1375 |
| BsiSI | CCGG | 1 cut(s) | 1832 |
| BslFI | GGGAC | 3 cut(s) | 1357, 1694, 1814 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 88, 266, 475, 1063, 1799, 1929 |
| BsmAI | GTCTC | 1 cut(s) | 376 |
| BsmBI | CGTCTC | 1 cut(s) | 376 |
| BsmFI | GGGAC | 3 cut(s) | 1357, 1694, 1814 |
| BsmI | GAATGC | 1 cut(s) | 120 |
| BsnI | GGCC | 1 cut(s) | 688 |
| Bsp119I | TTCGAA | 1 cut(s) | 700 |
| Bsp1286I | GDGCHC | 2 cut(s) | 871, 1375 |
| Bsp1407I | TGTACA | 1 cut(s) | 733 |
| Bsp143I | GATC | 6 cut(s) | 78, 487, 522, 1351, 1489, 1807 |
| BspACI | CCGC | 1 cut(s) | 1863 |
| BspANI | GGCC | 1 cut(s) | 688 |
| BspCNI | CTCAG | 5 cut(s) | 387, 921, 927, 1021, 1347 |
| BspFNI | CGCG | 1 cut(s) | 1311 |
| BspHI | TCATGA | 1 cut(s) | 208 |
| BspLI | GGNNCC | 5 cut(s) | 80, 418, 507, 1125, 1710 |
| BspMAI | CTGCAG | 1 cut(s) | 1132 |
| BspPI | GGATC | 3 cut(s) | 73, 86, 1484 |
| BspT104I | TTCGAA | 1 cut(s) | 700 |
| BspTI | CTTAAG | 1 cut(s) | 284 |
| BsrDI | GCAATG | 2 cut(s) | 666, 1662 |
| BsrGI | TGTACA | 1 cut(s) | 733 |
| BsrI | ACTGG | 4 cut(s) | 54, 480, 1433, 1734 |
| BssECI | CCNNGG | 1 cut(s) | 225 |
| BssMI | GATC | 6 cut(s) | 78, 487, 522, 1351, 1489, 1807 |
| BssNI | GRCGYC | 1 cut(s) | 553 |
| Bst2UI | CCWGG | 5 cut(s) | 227, 549, 557, 1064, 1451 |
| Bst4CI | ACNGT | 4 cut(s) | 161, 850, 1395, 1461 |
| Bst6I | CTCTTC | 3 cut(s) | 430, 737, 1446 |
| BstACI | GRCGYC | 1 cut(s) | 553 |
| BstAFI | CTTAAG | 1 cut(s) | 284 |
| BstAUI | TGTACA | 1 cut(s) | 733 |
| BstBI | TTCGAA | 1 cut(s) | 700 |
| BstC8I | GCNNGC | 6 cut(s) | 322, 415, 1026, 1096, 1514, 1565 |
| BstDEI | CTNAG | 8 cut(s) | 374, 914, 923, 929, 974, 1029, 1229, 1355 |
| BstEII | GGTNACC | 1 cut(s) | 161 |
| BstF5I | GGATG | 3 cut(s) | 469, 1789, 1876 |
| BstFNI | CGCG | 1 cut(s) | 1311 |
| BstHHI | GCGC | 2 cut(s) | 120, 1313 |
| BstKTI | GATC | 6 cut(s) | 81, 490, 525, 1354, 1492, 1810 |
| BstMAI | GTCTC | 1 cut(s) | 376 |
| BstMBI | GATC | 6 cut(s) | 78, 487, 522, 1351, 1489, 1807 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 414, 538, 569, 1202 |
| BstNI | CCWGG | 5 cut(s) | 227, 549, 557, 1064, 1451 |
| BstPI | GGTNACC | 1 cut(s) | 161 |
| BstSCI | CCNGG | 6 cut(s) | 225, 547, 555, 1062, 1449, 1830 |
| BstSFI | CTRYAG | 2 cut(s) | 828, 1128 |
| BstSLI | GKGCMC | 1 cut(s) | 871 |
| BstUI | CGCG | 1 cut(s) | 1311 |
| BstV1I | GCAGC | 4 cut(s) | 322, 494, 572, 668 |
| BstV2I | GAAGAC | 3 cut(s) | 162, 709, 827 |
| BstX2I | RGATCY | 2 cut(s) | 78, 1489 |
| BstYI | RGATCY | 2 cut(s) | 78, 1489 |
| Bsu36I | CCTNAGG | 1 cut(s) | 929 |
| BsuI | GTATCC | 1 cut(s) | 611 |
| BsuRI | GGCC | 1 cut(s) | 688 |
| BtrI | CACGTC | 1 cut(s) | 386 |
| BtsCI | GGATG | 3 cut(s) | 469, 1789, 1876 |
| BtsIMutI | CAGTG | 1 cut(s) | 1426 |
| Cac8I | GCNNGC | 6 cut(s) | 322, 415, 1026, 1096, 1514, 1565 |
| CaiI | CAGNNNCTG | 3 cut(s) | 575, 991, 1393 |
| CciI | TCATGA | 1 cut(s) | 208 |
| CfoI | GCGC | 2 cut(s) | 120, 1313 |
| Cfr13I | GGNCC | 3 cut(s) | 994, 1453, 1708 |
| CseI | GACGC | 2 cut(s) | 398, 561 |
| CsiI | ACCWGGT | 1 cut(s) | 1449 |
| Csp6I | GTAC | 5 cut(s) | 276, 734, 1396, 1526, 1914 |
| CviAII | CATG | 8 cut(s) | 100, 209, 326, 542, 1010, 1780, 1826, 1910 |
| CviQI | GTAC | 5 cut(s) | 276, 734, 1396, 1526, 1914 |
| DdeI | CTNAG | 8 cut(s) | 374, 914, 923, 929, 974, 1029, 1229, 1355 |
| DpnI | GATC | 6 cut(s) | 80, 489, 524, 1353, 1491, 1809 |
| DpnII | GATC | 6 cut(s) | 78, 487, 522, 1351, 1489, 1807 |
| DraI | TTTAAA | 1 cut(s) | 1248 |
| EaeI | YGGCCR | 1 cut(s) | 686 |
| Eam1104I | CTCTTC | 3 cut(s) | 430, 737, 1446 |
| EarI | CTCTTC | 3 cut(s) | 430, 737, 1446 |
| Eco47I | GGWCC | 3 cut(s) | 994, 1453, 1708 |
| Eco57I | CTGAAG | 4 cut(s) | 821, 1311, 1370, 1616 |
| Eco81I | CCTNAGG | 1 cut(s) | 929 |
| Eco91I | GGTNACC | 1 cut(s) | 161 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1708 |
| EcoO65I | GGTNACC | 1 cut(s) | 161 |
| EcoRI | GAATTC | 1 cut(s) | 1724 |
| EcoRII | CCWGG | 5 cut(s) | 225, 547, 555, 1062, 1449 |
| Esp3I | CGTCTC | 1 cut(s) | 376 |
| FaeI | CATG | 8 cut(s) | 103, 212, 329, 545, 1013, 1783, 1829, 1913 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 780, 812 |
| FaqI | GGGAC | 3 cut(s) | 1357, 1694, 1814 |
| FatI | CATG | 8 cut(s) | 99, 208, 325, 541, 1009, 1779, 1825, 1909 |
| FauNDI | CATATG | 1 cut(s) | 1714 |
| FblI | GTMKAC | 1 cut(s) | 1875 |
| Fnu4HI | GCNGC | 4 cut(s) | 336, 483, 561, 657 |
| FokI | GGATG | 3 cut(s) | 476, 1776, 1883 |
| Fsp4HI | GCNGC | 4 cut(s) | 336, 483, 561, 657 |
| FspBI | CTAG | 4 cut(s) | 1271, 1604, 1667, 1960 |
| GlaI | GCGC | 2 cut(s) | 119, 1312 |
| GluI | GCNGC | 4 cut(s) | 336, 483, 561, 657 |
| HaeIII | GGCC | 1 cut(s) | 688 |
| HapII | CCGG | 1 cut(s) | 1832 |
| HgaI | GACGC | 2 cut(s) | 398, 561 |
| HhaI | GCGC | 2 cut(s) | 120, 1313 |
| Hin1I | GRCGYC | 1 cut(s) | 553 |
| Hin1II | CATG | 8 cut(s) | 103, 212, 329, 545, 1013, 1783, 1829, 1913 |
| Hin6I | GCGC | 2 cut(s) | 118, 1311 |
| HinP1I | GCGC | 2 cut(s) | 118, 1311 |
| HincII | GTYRAC | 1 cut(s) | 718 |
| HindII | GTYRAC | 1 cut(s) | 718 |
| HindIII | AAGCTT | 4 cut(s) | 290, 318, 1561, 1673 |
| HinfI | GANTC | 8 cut(s) | 585, 748, 988, 1039, 1085, 1222, 1474, 1818 |
| HpaI | GTTAAC | 1 cut(s) | 718 |
| HpaII | CCGG | 1 cut(s) | 1832 |
| HphI | GGTGA | 3 cut(s) | 73, 173, 955 |
| Hpy166II | GTNNAC | 5 cut(s) | 64, 718, 794, 869, 1876 |
| Hpy188I | TCNGA | 8 cut(s) | 197, 368, 377, 435, 802, 1356, 1457, 1596 |
| Hpy188III | TCNNGA | 9 cut(s) | 209, 967, 992, 1184, 1329, 1376, 1532, 1667, 1793 |
| Hpy8I | GTNNAC | 5 cut(s) | 64, 718, 794, 869, 1876 |
| Hpy99I | CGWCG | 1 cut(s) | 435 |
| HpyAV | CCTTC | 9 cut(s) | 47, 341, 782, 1316, 1330, 1334, 1477, 1699, 1948 |
| HpyCH4III | ACNGT | 4 cut(s) | 161, 850, 1395, 1461 |
| HpyCH4IV | ACGT | 2 cut(s) | 385, 445 |
| HpyCH4V | TGCA | 9 cut(s) | 149, 300, 656, 727, 862, 869, 1094, 1130, 1567 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 414, 538, 569, 1202 |
| HpyF3I | CTNAG | 8 cut(s) | 374, 914, 923, 929, 974, 1029, 1229, 1355 |
| HpySE526I | ACGT | 2 cut(s) | 385, 445 |
| Hsp92I | GRCGYC | 1 cut(s) | 553 |
| Hsp92II | CATG | 8 cut(s) | 103, 212, 329, 545, 1013, 1783, 1829, 1913 |
| HspAI | GCGC | 2 cut(s) | 118, 1311 |
| KspAI | GTTAAC | 1 cut(s) | 718 |
| Kzo9I | GATC | 6 cut(s) | 78, 487, 522, 1351, 1489, 1807 |
| LmnI | GCTCC | 4 cut(s) | 422, 505, 1378, 1652 |
| Lsp1109I | GCAGC | 4 cut(s) | 322, 494, 572, 668 |
| LweI | GCATC | 4 cut(s) | 136, 211, 309, 454 |
| MabI | ACCWGGT | 1 cut(s) | 1449 |
| MaeI | CTAG | 4 cut(s) | 1271, 1604, 1667, 1960 |
| MaeII | ACGT | 2 cut(s) | 385, 445 |
| MaeIII | GTNAC | 6 cut(s) | 16, 42, 161, 386, 446, 1599 |
| MalI | GATC | 6 cut(s) | 80, 489, 524, 1353, 1491, 1809 |
| MboI | GATC | 6 cut(s) | 78, 487, 522, 1351, 1489, 1807 |
| MfeI | CAATTG | 2 cut(s) | 1347, 1631 |
| MflI | RGATCY | 2 cut(s) | 78, 1489 |
| MhlI | GDGCHC | 2 cut(s) | 871, 1375 |
| MlsI | TGGCCA | 1 cut(s) | 688 |
| MluNI | TGGCCA | 1 cut(s) | 688 |
| MlyI | GAGTC | 7 cut(s) | 594, 742, 982, 1094, 1216, 1483, 1827 |
| MmeI | TCCRAC | 4 cut(s) | 413, 980, 1480, 1708 |
| Mox20I | TGGCCA | 1 cut(s) | 688 |
| MroXI | GAANNNNTTC | 3 cut(s) | 696, 1383, 1626 |
| MscI | TGGCCA | 1 cut(s) | 688 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1008, 1794 |
| Msp20I | TGGCCA | 1 cut(s) | 688 |
| MspA1I | CMGCKG | 3 cut(s) | 485, 520, 1028 |
| MspCI | CTTAAG | 1 cut(s) | 284 |
| MspI | CCGG | 1 cut(s) | 1832 |
| MspR9I | CCNGG | 6 cut(s) | 227, 549, 557, 1064, 1451, 1832 |
| MunI | CAATTG | 2 cut(s) | 1347, 1631 |
| Mva1269I | GAATGC | 1 cut(s) | 120 |
| MvaI | CCWGG | 5 cut(s) | 227, 549, 557, 1064, 1451 |
| MvnI | CGCG | 1 cut(s) | 1311 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 414, 538, 569, 1202 |
| NciI | CCSGG | 1 cut(s) | 1832 |
| NdeI | CATATG | 1 cut(s) | 1714 |
| NdeII | GATC | 6 cut(s) | 78, 487, 522, 1351, 1489, 1807 |
| NlaIII | CATG | 8 cut(s) | 103, 212, 329, 545, 1013, 1783, 1829, 1913 |
| NlaIV | GGNNCC | 5 cut(s) | 80, 418, 507, 1125, 1710 |
| NmuCI | GTSAC | 5 cut(s) | 16, 42, 161, 386, 446 |
| NspV | TTCGAA | 1 cut(s) | 700 |
| PagI | TCATGA | 1 cut(s) | 208 |
| PctI | GAATGC | 1 cut(s) | 120 |
| PdmI | GAANNNNTTC | 3 cut(s) | 696, 1383, 1626 |
| PfeI | GAWTC | 1 cut(s) | 1039 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1063, 1799 |
| PkrI | GCNGC | 4 cut(s) | 337, 484, 562, 658 |
| PleI | GAGTC | 7 cut(s) | 593, 742, 982, 1093, 1216, 1482, 1826 |
| PpsI | GAGTC | 7 cut(s) | 593, 742, 982, 1093, 1216, 1482, 1826 |
| PpuMI | RGGWCCY | 1 cut(s) | 1708 |
| PshBI | ATTAAT | 1 cut(s) | 1775 |
| Psp5II | RGGWCCY | 1 cut(s) | 1708 |
| Psp6I | CCWGG | 5 cut(s) | 225, 547, 555, 1062, 1449 |
| PspEI | GGTNACC | 1 cut(s) | 161 |
| PspGI | CCWGG | 5 cut(s) | 225, 547, 555, 1062, 1449 |
| PspN4I | GGNNCC | 5 cut(s) | 80, 418, 507, 1125, 1710 |
| PspPI | GGNCC | 3 cut(s) | 994, 1453, 1708 |
| PspPPI | RGGWCCY | 1 cut(s) | 1708 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1929, 1961 |
| PstI | CTGCAG | 1 cut(s) | 1132 |
| PstNI | CAGNNNCTG | 3 cut(s) | 575, 991, 1393 |
| PsuI | RGATCY | 2 cut(s) | 78, 1489 |
| PvuII | CAGCTG | 3 cut(s) | 485, 520, 1028 |
| RsaI | GTAC | 5 cut(s) | 277, 735, 1397, 1527, 1915 |
| RsaNI | GTAC | 5 cut(s) | 276, 734, 1396, 1526, 1914 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1008, 1794 |
| SatI | GCNGC | 4 cut(s) | 336, 483, 561, 657 |
| Sau3AI | GATC | 6 cut(s) | 78, 487, 522, 1351, 1489, 1807 |
| Sau96I | GGNCC | 3 cut(s) | 994, 1453, 1708 |
| SbfI | CCTGCAGG | 1 cut(s) | 1132 |
| SchI | GAGTC | 7 cut(s) | 594, 742, 982, 1094, 1216, 1483, 1827 |
| ScrFI | CCNGG | 6 cut(s) | 227, 549, 557, 1064, 1451, 1832 |
| SdaI | CCTGCAGG | 1 cut(s) | 1132 |
| SduI | GDGCHC | 2 cut(s) | 871, 1375 |
| SexAI | ACCWGGT | 1 cut(s) | 1449 |
| SfaNI | GCATC | 4 cut(s) | 136, 211, 309, 454 |
| SfcI | CTRYAG | 2 cut(s) | 828, 1128 |
| SfuI | TTCGAA | 1 cut(s) | 700 |
| SinI | GGWCC | 3 cut(s) | 994, 1453, 1708 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1008, 1794 |
| SmlI | CTYRAG | 2 cut(s) | 284, 1043 |
| SmoI | CTYRAG | 2 cut(s) | 284, 1043 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 1132 |
| SsiI | CCGC | 1 cut(s) | 1863 |
| SspMI | CTAG | 4 cut(s) | 1271, 1604, 1667, 1960 |
| StyD4I | CCNGG | 6 cut(s) | 225, 547, 555, 1062, 1449, 1830 |
| TaaI | ACNGT | 4 cut(s) | 161, 850, 1395, 1461 |
| TaiI | ACGT | 2 cut(s) | 388, 448 |
| TaqI | TCGA | 4 cut(s) | 525, 700, 1758, 1935 |
| TatI | WGTACW | 2 cut(s) | 733, 1395 |
| TfiI | GAWTC | 1 cut(s) | 1039 |
| TscAI | CASTG | 1 cut(s) | 1433 |
| TseFI | GTSAC | 5 cut(s) | 16, 42, 161, 386, 446 |
| TseI | GCWGC | 4 cut(s) | 335, 482, 560, 656 |
| Tsp45I | GTSAC | 5 cut(s) | 16, 42, 161, 386, 446 |
| TspDTI | ATGAA | 8 cut(s) | 121, 123, 342, 551, 1092, 1313, 1785, 1895 |
| TspRI | CASTG | 1 cut(s) | 1433 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1063, 1799 |
| Vha464I | CTTAAG | 1 cut(s) | 284 |
| VneI | GTGCAC | 1 cut(s) | 867 |
| VpaK11BI | GGWCC | 3 cut(s) | 994, 1453, 1708 |
| VspI | ATTAAT | 1 cut(s) | 1775 |
| XapI | RAATTY | 7 cut(s) | 233, 649, 951, 1379, 1519, 1724, 1751 |
| XbaI | TCTAGA | 1 cut(s) | 1666 |
| XmiI | GTMKAC | 1 cut(s) | 1875 |
| XmnI | GAANNNNTTC | 3 cut(s) | 696, 1383, 1626 |
| XspI | CTAG | 4 cut(s) | 1271, 1604, 1667, 1960 |