Rh2CG296700

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000482
Physical Location & Seq
Forward (+)
34218153 .. 34218521
369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG296700.1

Sequence Viewer

Length: 369 bp
ATGACGGCTTTCCTGAGGGTTGCTGATGCATATGAAGAGAGTGATGCGGAGCTCAAAGTATGGGTTAAACAAATAAGAGACATTGCTCACGAAAGTGAAGATGTTCTTGATGAATTTACCCTTCTGCAAGCACCTGATCATGGGGAGGGATTATATGGTTCAATCCATAAGCTTTCTTGCTGCATCAAGAATACAAAAGCTCGATATCGAATTGCTTCGGAGTTACGAGCCATAAAAACTCCAGGATCAAAAAAATCTCTGAGGTTCATAAGCGACTCCGCCACAAATTCAATATGGCTGAACAAGGCTCAAGCTCCAAAAGTGCAGGTTACCTGTTGGAGGACCATCGAGGTGATGCTTTACTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

122

Amino Acids

13.91

Weight (kDa)

7.72

Isoelectric Point (pI)

33.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 1 - 45 1.8e-11 Rx N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000482)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g23450 FvH4_5g23460 FvH4_5g23470 FvH4_5g24290 FvH4_5g24300
malus_domestica MD06G1024000.v1.1
prunus_persica Prupe.5G025000_v2.0.a1 Prupe.5G025000_v2.0.a1 Prupe.5G025100_v2.0.a1 Prupe.5G025200_v2.0.a1
pyrus_communis pycom16g25280
rosa_chinensis RchiOBHm_Chr7g0212511 RchiOBHm_Chr7g0212531 RchiOBHm_Chr7g0212541 RchiOBHm_Chr7g0212561 RchiOBHm_Chr7g0212571 RchiOBHm_Chr7g0212621 RchiOBHm_Chr7g0212631 RchiOBHm_Chr7g0212671 RchiOBHm_Chr7g0212691 RchiOBHm_Chr7g0212701
rosa_laevigata RLG00000002879 RLG00000002883 RLG00000002884 RLG00000002885
rosa_multiflora Rmu_co8121580.1_g000001 Rmu_sc0000394.1_g000014 Rmu_sc0000394.1_g000015 Rmu_sc0001435.1_g000001 Rmu_sc0005246.1_g000005 Rmu_sc0005246.1_g000010 Rmu_sc0005246.1_g000015 Rmu_sc0005246.1_g000016 Rmu_sc0005770.1_g000018 Rmu_sc0005770.1_g000019 Rmu_sc0005770.1_g000020 Rmu_sc0007576.1_g000019 Rmu_sc0009400.1_g000001 Rmu_sc0011082.1_g000001 Rmu_sc0038076.1_g000002
rosa_roxburghii Rroxscaffold_3G00246720
rosa_rugosa Rorug07G0134800 Rorug07G0134900 Rorug07G0135100 Rorug07G0135200 Rorug07G0135400 Rorug07G0135500 Rorug07G0135800 Rorug07G0135900
rosa_samantha Rh2AG310100 Rh2CG296700 Rh2CG297000 Rh2CG301700 Rh7BG258900 Rh7BG261600 Rh7BG261700 Rh7BG261800 Rh7BG261900 Rh7BG262000 Rh7BG262100 Rh7BG262400 Rh7BG262500 Rh7BG263100 Rh7BG263200 Rh7CG284400 Rh7CG284600 Rh7CG284700 Rh7CG284900 Rh7CG285100 Rh7CG285500 Rh7CG285600 Rh7CG285700 Rh7CG286200 Rh7CG286600 Rh7CG286700 Rh7DG274400 Rh7DG274600 Rh7DG274800 Rh7DG274900 Rh7DG275100 Rh7DG275200 Rh7DG275500 Rh7DG275600 Rh7DG275700
rosa_wichuraiana Rw7G022910 Rw7G022960 Rw7G022980 Rw7G023010 Rw7G023050 Rw7G023110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 316
AciI CCGC 2 cut(s) 47, 279
AclWI GGATC 1 cut(s) 253
AcsI RAATTY 2 cut(s) 113, 286
AfiI CCNNNNNNNGG 2 cut(s) 140, 339
AgsI TTSAA 2 cut(s) 162, 291
AjnI CCWGG 1 cut(s) 241
AleI CACNNNNGTG 1 cut(s) 93
AluBI AGCT 4 cut(s) 52, 172, 200, 314
AluI AGCT 4 cut(s) 52, 172, 200, 314
Alw21I GWGCWC 1 cut(s) 54
Alw26I GTCTC 1 cut(s) 72
AlwI GGATC 1 cut(s) 253
ApeKI GCWGC 1 cut(s) 180
ApoI RAATTY 2 cut(s) 113, 286
Asp700I GAANNNNTTC 2 cut(s) 102, 214
AspS9I GGNCC 1 cut(s) 342
AsuHPI GGTGA 1 cut(s) 364
AvaII GGWCC 1 cut(s) 342
AxyI CCTNAGG 1 cut(s) 14
BanII GRGCYC 1 cut(s) 54
Bbv12I GWGCWC 1 cut(s) 54
BbvI GCAGC 1 cut(s) 167
BccI CCATC 1 cut(s) 353
BceAI ACGGC 1 cut(s) 21
BciT130I CCWGG 1 cut(s) 243
BclI TGATCA 1 cut(s) 136
BcoDI GTCTC 1 cut(s) 72
BfuAI ACCTGC 1 cut(s) 316
BisI GCNGC 1 cut(s) 181
BlsI GCNGC 1 cut(s) 182
Bme1390I CCNGG 1 cut(s) 243
Bme18I GGWCC 1 cut(s) 342
BmgT120I GGNCC 1 cut(s) 342
BmrFI CCNGG 1 cut(s) 243
BmsI GCATC 4 cut(s) 16, 34, 192, 345
BpmI CTGGAG 1 cut(s) 225
BpuEI CTTGAG 1 cut(s) 294
Bsc4I CCNNNNNNNGG 2 cut(s) 140, 339
Bse21I CCTNAGG 1 cut(s) 14
Bse3DI GCAATG 1 cut(s) 81
BseBI CCWGG 1 cut(s) 243
BseLI CCNNNNNNNGG 2 cut(s) 140, 339
BseMI GCAATG 1 cut(s) 81
BseMII CTCAG 2 cut(s) 5, 251
BseXI GCAGC 1 cut(s) 167
BsgI GTGCAG 1 cut(s) 344
BsiHKAI GWGCWC 1 cut(s) 54
BslI CCNNNNNNNGG 2 cut(s) 140, 339
BsmAI GTCTC 1 cut(s) 72
Bsp1286I GDGCHC 1 cut(s) 54
Bsp143I GATC 2 cut(s) 136, 245
BspACI CCGC 2 cut(s) 47, 279
BspCNI CTCAG 2 cut(s) 6, 252
BspMI ACCTGC 1 cut(s) 316
BspPI GGATC 1 cut(s) 253
BsrDI GCAATG 1 cut(s) 81
BssMI GATC 2 cut(s) 136, 245
Bst2UI CCWGG 1 cut(s) 243
Bst6I CTCTTC 1 cut(s) 30
BstC8I GCNNGC 1 cut(s) 129
BstDEI CTNAG 2 cut(s) 14, 260
BstEII GGTNACC 1 cut(s) 328
BstENI CCTNNNNNAGG 1 cut(s) 337
BstKTI GATC 2 cut(s) 139, 248
BstMAI GTCTC 1 cut(s) 72
BstMBI GATC 2 cut(s) 136, 245
BstNI CCWGG 1 cut(s) 243
BstPI GGTNACC 1 cut(s) 328
BstSCI CCNGG 1 cut(s) 241
BstV1I GCAGC 1 cut(s) 167
Bsu36I CCTNAGG 1 cut(s) 14
BveI ACCTGC 1 cut(s) 316
Cac8I GCNNGC 1 cut(s) 129
Cfr13I GGNCC 1 cut(s) 342
CviAII CATG 1 cut(s) 140
CviJI RGCY 8 cut(s) 8, 52, 172, 200, 230, 298, 308, 314
CviKI_1 RGCY 8 cut(s) 8, 52, 172, 200, 230, 298, 308, 314
DdeI CTNAG 2 cut(s) 14, 260
DpnI GATC 2 cut(s) 138, 247
DpnII GATC 2 cut(s) 136, 245
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
EciI GGCGGA 1 cut(s) 268
Ecl136II GAGCTC 1 cut(s) 52
Eco24I GRGCYC 1 cut(s) 54
Eco32I GATATC 1 cut(s) 206
Eco47I GGWCC 1 cut(s) 342
Eco53kI GAGCTC 1 cut(s) 52
Eco81I CCTNAGG 1 cut(s) 14
Eco91I GGTNACC 1 cut(s) 328
EcoICRI GAGCTC 1 cut(s) 52
EcoNI CCTNNNNNAGG 1 cut(s) 337
EcoO65I GGTNACC 1 cut(s) 328
EcoRII CCWGG 1 cut(s) 241
EcoRV GATATC 1 cut(s) 206
EcoT22I ATGCAT 1 cut(s) 31
EcoT38I GRGCYC 1 cut(s) 54
FaeI CATG 1 cut(s) 143
FalI AAGNNNNNCTT 2 cut(s) 90, 122
FatI CATG 1 cut(s) 139
FauNDI CATATG 1 cut(s) 31
FbaI TGATCA 1 cut(s) 136
Fnu4HI GCNGC 1 cut(s) 181
FriOI GRGCYC 1 cut(s) 54
Fsp4HI GCNGC 1 cut(s) 181
GluI GCNGC 1 cut(s) 181
GsuI CTGGAG 1 cut(s) 225
Hin1II CATG 1 cut(s) 143
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 1 cut(s) 275
HphI GGTGA 1 cut(s) 364
Hpy188I TCNGA 2 cut(s) 220, 261
Hpy188III TCNNGA 4 cut(s) 13, 89, 107, 187
HpyAV CCTTC 1 cut(s) 131
HpyCH4V TGCA 4 cut(s) 29, 127, 183, 325
HpyF3I CTNAG 2 cut(s) 14, 260
Hsp92II CATG 1 cut(s) 143
Ksp22I TGATCA 1 cut(s) 136
Kzo9I GATC 2 cut(s) 136, 245
LmnI GCTCC 2 cut(s) 49, 319
LpnPI CCDG 6 cut(s) 26, 147, 228, 255, 311, 346
Lsp1109I GCAGC 1 cut(s) 167
LweI GCATC 4 cut(s) 16, 34, 192, 345
MaeIII GTNAC 2 cut(s) 222, 328
MalI GATC 2 cut(s) 138, 247
MboI GATC 2 cut(s) 136, 245
MboII GAAGA 2 cut(s) 47, 110
MhlI GDGCHC 1 cut(s) 54
MluCI AATT 3 cut(s) 113, 210, 286
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 1 cut(s) 317
MnlI CCTC 5 cut(s) 9, 139, 255, 333, 343
Mph1103I ATGCAT 1 cut(s) 31
MroXI GAANNNNTTC 2 cut(s) 102, 214
MseI TTAA 1 cut(s) 66
MslI CAYNNNNRTG 2 cut(s) 93, 350
MspR9I CCNGG 1 cut(s) 243
MvaI CCWGG 1 cut(s) 243
NdeI CATATG 1 cut(s) 31
NdeII GATC 2 cut(s) 136, 245
NlaIII CATG 1 cut(s) 143
NsiI ATGCAT 1 cut(s) 31
OliI CACNNNNGTG 1 cut(s) 93
PdmI GAANNNNTTC 2 cut(s) 102, 214
PfoI TCCNGGA 1 cut(s) 241
PkrI GCNGC 1 cut(s) 182
PleI GAGTC 1 cut(s) 269
PpsI GAGTC 1 cut(s) 269
Psp124BI GAGCTC 1 cut(s) 54
Psp6I CCWGG 1 cut(s) 241
PspEI GGTNACC 1 cut(s) 328
PspGI CCWGG 1 cut(s) 241
PspPI GGNCC 1 cut(s) 342
RseI CAYNNNNRTG 2 cut(s) 93, 350
SacI GAGCTC 1 cut(s) 54
SaqAI TTAA 1 cut(s) 66
SatI GCNGC 1 cut(s) 181
Sau3AI GATC 2 cut(s) 136, 245
Sau96I GGNCC 1 cut(s) 342
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 1 cut(s) 243
SduI GDGCHC 1 cut(s) 54
SetI ASST 9 cut(s) 54, 136, 174, 202, 266, 316, 330, 335, 354
SfaNI GCATC 4 cut(s) 16, 34, 192, 345
SinI GGWCC 1 cut(s) 342
SmiMI CAYNNNNRTG 2 cut(s) 93, 350
SmlI CTYRAG 1 cut(s) 309
SmoI CTYRAG 1 cut(s) 309
Sse9I AATT 3 cut(s) 113, 210, 286
SsiI CCGC 2 cut(s) 47, 279
SstI GAGCTC 1 cut(s) 54
StyD4I CCNGG 1 cut(s) 241
TaqI TCGA 3 cut(s) 202, 208, 348
TasI AATT 3 cut(s) 113, 210, 286
Tru1I TTAA 1 cut(s) 66
Tru9I TTAA 1 cut(s) 66
TseI GCWGC 1 cut(s) 180
TspDTI ATGAA 3 cut(s) 48, 126, 256
VpaK11BI GGWCC 1 cut(s) 342
XagI CCTNNNNNAGG 1 cut(s) 337
XapI RAATTY 2 cut(s) 113, 286
XmnI GAANNNNTTC 2 cut(s) 102, 214
Zsp2I ATGCAT 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.