Belongs to the disease resistance NB-LRR family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 2763 |
| Acc36I | ACCTGC | 1 cut(s) | 1921 |
| Acc65I | GGTACC | 1 cut(s) | 822 |
| AccB1I | GGYRCC | 1 cut(s) | 822 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 572, 2687 |
| AccI | GTMKAC | 1 cut(s) | 615 |
| AciI | CCGC | 1 cut(s) | 2575 |
| AclI | AACGTT | 1 cut(s) | 640 |
| AclWI | GGATC | 8 cut(s) | 73, 86, 617, 630, 668, 1071, 2372, 2385 |
| AcoI | YGGCCR | 1 cut(s) | 1119 |
| AcsI | RAATTY | 5 cut(s) | 233, 330, 1057, 1839, 2639 |
| AcuI | CTGAAG | 6 cut(s) | 768, 1377, 1709, 2258, 2378, 2504 |
| AcyI | GRCGYC | 2 cut(s) | 553, 2760 |
| AfaI | GTAC | 8 cut(s) | 651, 824, 1134, 1567, 1623, 1808, 2388, 2415 |
| AfeI | AGCGCT | 1 cut(s) | 1129 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 88, 135, 379, 475, 572, 739, 787, 2687 |
| AflII | CTTAAG | 1 cut(s) | 133 |
| AgeI | ACCGGT | 2 cut(s) | 739, 1718 |
| AjnI | CCWGG | 5 cut(s) | 99, 555, 1950, 2337, 2409 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 584, 616 |
| Alw21I | GWGCWC | 3 cut(s) | 1759, 2100, 2263 |
| Alw26I | GTCTC | 1 cut(s) | 1868 |
| Alw44I | GTGCAC | 1 cut(s) | 1755 |
| AlwI | GGATC | 8 cut(s) | 73, 86, 617, 630, 668, 1071, 2372, 2385 |
| Ama87I | CYCGRG | 1 cut(s) | 2099 |
| Aor51HI | AGCGCT | 1 cut(s) | 1129 |
| AoxI | GGCC | 3 cut(s) | 126, 1119, 1574 |
| ApaLI | GTGCAC | 1 cut(s) | 1755 |
| ApeKI | GCWGC | 7 cut(s) | 808, 1172, 1400, 1448, 1544, 1612, 2632 |
| ApoI | RAATTY | 5 cut(s) | 233, 330, 1057, 1839, 2639 |
| AseI | ATTAAT | 2 cut(s) | 1337, 2663 |
| AsiGI | ACCGGT | 2 cut(s) | 739, 1718 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 641 |
| Asp718I | GGTACC | 1 cut(s) | 822 |
| AspLEI | GCGC | 4 cut(s) | 120, 444, 510, 1130 |
| AspS9I | GGNCC | 4 cut(s) | 97, 1882, 2011, 2596 |
| AsuHPI | GGTGA | 5 cut(s) | 10, 73, 910, 1843, 2152 |
| AsuII | TTCGAA | 1 cut(s) | 1588 |
| AvaI | CYCGRG | 1 cut(s) | 2099 |
| AvaII | GGWCC | 4 cut(s) | 97, 1882, 2011, 2596 |
| BaeGI | GKGCMC | 1 cut(s) | 1759 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1790, 1823 |
| BalI | TGGCCA | 1 cut(s) | 1121 |
| BamHI | GGATCC | 3 cut(s) | 78, 622, 2377 |
| BanI | GGYRCC | 1 cut(s) | 822 |
| BanII | GRGCYC | 2 cut(s) | 990, 2100 |
| BauI | CACGAG | 1 cut(s) | 2256 |
| BbsI | GAAGAC | 5 cut(s) | 162, 549, 1034, 1597, 1715 |
| Bbv12I | GWGCWC | 3 cut(s) | 1759, 2100, 2263 |
| BbvCI | CCTCAGC | 1 cut(s) | 168 |
| BbvI | GCAGC | 7 cut(s) | 795, 1159, 1387, 1460, 1556, 1599, 2619 |
| BceAI | ACGGC | 1 cut(s) | 175 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1440, 1474 |
| BciT130I | CCWGG | 5 cut(s) | 101, 557, 1952, 2339, 2411 |
| BclI | TGATCA | 3 cut(s) | 454, 708, 1213 |
| BcoDI | GTCTC | 1 cut(s) | 1868 |
| BfaI | CTAG | 5 cut(s) | 50, 1136, 1527, 1871, 2555 |
| BfmI | CTRYAG | 4 cut(s) | 809, 1401, 1613, 2016 |
| BfoI | RGCGCY | 2 cut(s) | 445, 1131 |
| BfrI | CTTAAG | 1 cut(s) | 133 |
| BfuAI | ACCTGC | 1 cut(s) | 1921 |
| BisI | GCNGC | 7 cut(s) | 809, 1173, 1401, 1449, 1545, 1613, 2633 |
| BlsI | GCNGC | 7 cut(s) | 810, 1174, 1402, 1450, 1546, 1614, 2634 |
| Bme1390I | CCNGG | 5 cut(s) | 101, 557, 1952, 2339, 2411 |
| Bme18I | GGWCC | 4 cut(s) | 97, 1882, 2011, 2596 |
| BmeT110I | CYCGRG | 1 cut(s) | 2099 |
| BmgT120I | GGNCC | 4 cut(s) | 97, 1882, 2011, 2596 |
| BmrFI | CCNGG | 5 cut(s) | 101, 557, 1952, 2339, 2411 |
| BmrI | ACTGGG | 2 cut(s) | 596, 871 |
| BmsI | GCATC | 7 cut(s) | 129, 136, 211, 454, 856, 1308, 1497 |
| BmuI | ACTGGG | 2 cut(s) | 596, 871 |
| BpiI | GAAGAC | 5 cut(s) | 162, 549, 1034, 1597, 1715 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 900, 932 |
| BpmI | CTGGAG | 1 cut(s) | 981 |
| Bpu10I | CCTNAGC | 4 cut(s) | 168, 785, 1811, 2243 |
| Bpu14I | TTCGAA | 1 cut(s) | 1588 |
| BpuEI | CTTGAG | 3 cut(s) | 1787, 1837, 1916 |
| BsaHI | GRCGYC | 2 cut(s) | 553, 2760 |
| BsaI | GGTCTC | 1 cut(s) | 1868 |
| BsaJI | CCNNGG | 3 cut(s) | 600, 768, 1878 |
| BsaWI | WCCGGW | 3 cut(s) | 739, 856, 1718 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1995, 2025 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 88, 135, 379, 475, 572, 739, 787, 2687 |
| Bse118I | RCCGGY | 3 cut(s) | 413, 739, 1718 |
| Bse1I | ACTGG | 6 cut(s) | 480, 553, 566, 591, 866, 2321 |
| Bse3DI | GCAATG | 2 cut(s) | 934, 2550 |
| BseBI | CCWGG | 5 cut(s) | 101, 557, 1952, 2339, 2411 |
| BseDI | CCNNGG | 3 cut(s) | 600, 768, 1878 |
| BseGI | GGATG | 8 cut(s) | 469, 871, 1080, 1168, 1488, 1640, 2515, 2677 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 88, 135, 379, 475, 572, 739, 787, 2687 |
| BseMI | GCAATG | 2 cut(s) | 934, 2550 |
| BseMII | CTCAG | 6 cut(s) | 182, 1261, 1286, 1429, 1569, 1908 |
| BseNI | ACTGG | 6 cut(s) | 480, 553, 566, 591, 866, 2321 |
| BseRI | GAGGAG | 3 cut(s) | 2253, 2549, 2830 |
| BseSI | GKGCMC | 1 cut(s) | 1759 |
| BseXI | GCAGC | 7 cut(s) | 795, 1159, 1387, 1460, 1556, 1599, 2619 |
| BsgI | GTGCAG | 1 cut(s) | 33 |
| BshFI | GGCC | 3 cut(s) | 128, 1121, 1576 |
| BshNI | GGYRCC | 1 cut(s) | 822 |
| BshTI | ACCGGT | 2 cut(s) | 739, 1718 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1759, 2100, 2263 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2099 |
| BsiSI | CCGG | 5 cut(s) | 414, 420, 740, 857, 1719 |
| BslFI | GGGAC | 5 cut(s) | 396, 1113, 1997, 2582, 2771 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 88, 135, 379, 475, 572, 739, 787, 2687 |
| BsmAI | GTCTC | 1 cut(s) | 1868 |
| BsmFI | GGGAC | 5 cut(s) | 396, 1113, 1997, 2582, 2771 |
| BsnI | GGCC | 3 cut(s) | 128, 1121, 1576 |
| Bso31I | GGTCTC | 1 cut(s) | 1868 |
| BsoBI | CYCGRG | 1 cut(s) | 2099 |
| Bsp119I | TTCGAA | 1 cut(s) | 1588 |
| Bsp1286I | GDGCHC | 4 cut(s) | 990, 1759, 2100, 2263 |
| Bsp1407I | TGTACA | 1 cut(s) | 1621 |
| Bsp19I | CCATGG | 1 cut(s) | 768 |
| BspACI | CCGC | 1 cut(s) | 2575 |
| BspANI | GGCC | 3 cut(s) | 128, 1121, 1576 |
| BspCNI | CTCAG | 6 cut(s) | 181, 1260, 1287, 1428, 1570, 1909 |
| BspHI | TCATGA | 1 cut(s) | 208 |
| BspMAI | CTGCAG | 4 cut(s) | 813, 1405, 1617, 2020 |
| BspMI | ACCTGC | 1 cut(s) | 1921 |
| BspPI | GGATC | 8 cut(s) | 73, 86, 617, 630, 668, 1071, 2372, 2385 |
| BspT104I | TTCGAA | 1 cut(s) | 1588 |
| BspT107I | GGYRCC | 1 cut(s) | 822 |
| BspTI | CTTAAG | 1 cut(s) | 133 |
| BspTNI | GGTCTC | 1 cut(s) | 1868 |
| BsrDI | GCAATG | 2 cut(s) | 934, 2550 |
| BsrFI | RCCGGY | 3 cut(s) | 413, 739, 1718 |
| BsrGI | TGTACA | 1 cut(s) | 1621 |
| BsrI | ACTGG | 6 cut(s) | 480, 553, 566, 591, 866, 2321 |
| BssAI | RCCGGY | 3 cut(s) | 413, 739, 1718 |
| BssECI | CCNNGG | 3 cut(s) | 600, 768, 1878 |
| BssNI | GRCGYC | 2 cut(s) | 553, 2760 |
| BssSI | CACGAG | 1 cut(s) | 2256 |
| BssT1I | CCWWGG | 3 cut(s) | 600, 768, 1878 |
| Bst2BI | CACGAG | 1 cut(s) | 2256 |
| Bst2UI | CCWGG | 5 cut(s) | 101, 557, 1952, 2339, 2411 |
| Bst4CI | ACNGT | 5 cut(s) | 263, 667, 1256, 1738, 2349 |
| Bst6I | CTCTTC | 3 cut(s) | 51, 474, 2334 |
| BstACI | GRCGYC | 2 cut(s) | 553, 2760 |
| BstAFI | CTTAAG | 1 cut(s) | 133 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 11 |
| BstAUI | TGTACA | 1 cut(s) | 1621 |
| BstBI | TTCGAA | 1 cut(s) | 1588 |
| BstC8I | GCNNGC | 2 cut(s) | 506, 2453 |
| BstDSI | CCRYGG | 1 cut(s) | 768 |
| BstEII | GGTNACC | 1 cut(s) | 2158 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 133, 377 |
| BstF5I | GGATG | 8 cut(s) | 469, 871, 1080, 1168, 1488, 1640, 2515, 2677 |
| BstH2I | RGCGCY | 2 cut(s) | 445, 1131 |
| BstHHI | GCGC | 4 cut(s) | 120, 444, 510, 1130 |
| BstMAI | GTCTC | 1 cut(s) | 1868 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 11, 514, 1118, 1127, 1136, 2047, 2090 |
| BstNI | CCWGG | 5 cut(s) | 101, 557, 1952, 2339, 2411 |
| BstNSI | RCATGY | 3 cut(s) | 656, 973, 1901 |
| BstPI | GGTNACC | 1 cut(s) | 2158 |
| BstSCI | CCNGG | 5 cut(s) | 99, 555, 1950, 2337, 2409 |
| BstSFI | CTRYAG | 4 cut(s) | 809, 1401, 1613, 2016 |
| BstSLI | GKGCMC | 1 cut(s) | 1759 |
| BstV1I | GCAGC | 7 cut(s) | 795, 1159, 1387, 1460, 1556, 1599, 2619 |
| BstV2I | GAAGAC | 5 cut(s) | 162, 549, 1034, 1597, 1715 |
| BstX2I | RGATCY | 3 cut(s) | 78, 622, 2377 |
| BstYI | RGATCY | 3 cut(s) | 78, 622, 2377 |
| BsuRI | GGCC | 3 cut(s) | 128, 1121, 1576 |
| BtgI | CCRYGG | 1 cut(s) | 768 |
| BtsCI | GGATG | 8 cut(s) | 469, 871, 1080, 1168, 1488, 1640, 2515, 2677 |
| BtsIMutI | CAGTG | 4 cut(s) | 546, 663, 1261, 2314 |
| BveI | ACCTGC | 1 cut(s) | 1921 |
| Cac8I | GCNNGC | 2 cut(s) | 506, 2453 |
| CciI | TCATGA | 1 cut(s) | 208 |
| CfoI | GCGC | 4 cut(s) | 120, 444, 510, 1130 |
| Cfr10I | RCCGGY | 3 cut(s) | 413, 739, 1718 |
| Cfr13I | GGNCC | 4 cut(s) | 97, 1882, 2011, 2596 |
| CseI | GACGC | 1 cut(s) | 561 |
| CsiI | ACCWGGT | 3 cut(s) | 99, 2337, 2409 |
| Csp6I | GTAC | 8 cut(s) | 650, 823, 1133, 1566, 1622, 1807, 2387, 2414 |
| CspAI | ACCGGT | 2 cut(s) | 739, 1718 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 880, 915 |
| CviQI | GTAC | 8 cut(s) | 650, 823, 1133, 1566, 1622, 1807, 2387, 2414 |
| DraI | TTTAAA | 2 cut(s) | 1143, 2628 |
| EaeI | YGGCCR | 1 cut(s) | 1119 |
| Eam1104I | CTCTTC | 3 cut(s) | 51, 474, 2334 |
| EarI | CTCTTC | 3 cut(s) | 51, 474, 2334 |
| Ecl136II | GAGCTC | 1 cut(s) | 2098 |
| Eco130I | CCWWGG | 3 cut(s) | 600, 768, 1878 |
| Eco147I | AGGCCT | 1 cut(s) | 128 |
| Eco24I | GRGCYC | 2 cut(s) | 990, 2100 |
| Eco31I | GGTCTC | 1 cut(s) | 1868 |
| Eco47I | GGWCC | 4 cut(s) | 97, 1882, 2011, 2596 |
| Eco47III | AGCGCT | 1 cut(s) | 1129 |
| Eco53kI | GAGCTC | 1 cut(s) | 2098 |
| Eco57I | CTGAAG | 6 cut(s) | 768, 1377, 1709, 2258, 2378, 2504 |
| Eco88I | CYCGRG | 1 cut(s) | 2099 |
| Eco91I | GGTNACC | 1 cut(s) | 2158 |
| EcoICRI | GAGCTC | 1 cut(s) | 2098 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 133, 377 |
| EcoO109I | RGGNCCY | 3 cut(s) | 97, 2011, 2596 |
| EcoO65I | GGTNACC | 1 cut(s) | 2158 |
| EcoRII | CCWGG | 5 cut(s) | 99, 555, 1950, 2337, 2409 |
| EcoT14I | CCWWGG | 3 cut(s) | 600, 768, 1878 |
| EcoT38I | GRGCYC | 2 cut(s) | 990, 2100 |
| ErhI | CCWWGG | 3 cut(s) | 600, 768, 1878 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 116, 148, 1668, 1700, 2039, 2071 |
| FaqI | GGGAC | 5 cut(s) | 396, 1113, 1997, 2582, 2771 |
| FauNDI | CATATG | 1 cut(s) | 1329 |
| FbaI | TGATCA | 3 cut(s) | 454, 708, 1213 |
| FblI | GTMKAC | 1 cut(s) | 615 |
| Fnu4HI | GCNGC | 7 cut(s) | 809, 1173, 1401, 1449, 1545, 1613, 2633 |
| FokI | GGATG | 8 cut(s) | 476, 878, 1067, 1175, 1475, 1627, 2522, 2664 |
| FriOI | GRGCYC | 2 cut(s) | 990, 2100 |
| Fsp4HI | GCNGC | 7 cut(s) | 809, 1173, 1401, 1449, 1545, 1613, 2633 |
| FspBI | CTAG | 5 cut(s) | 50, 1136, 1527, 1871, 2555 |
| GlaI | GCGC | 4 cut(s) | 119, 443, 509, 1129 |
| GluI | GCNGC | 7 cut(s) | 809, 1173, 1401, 1449, 1545, 1613, 2633 |
| GsuI | CTGGAG | 1 cut(s) | 981 |
| HaeII | RGCGCY | 2 cut(s) | 445, 1131 |
| HaeIII | GGCC | 3 cut(s) | 128, 1121, 1576 |
| HapII | CCGG | 5 cut(s) | 414, 420, 740, 857, 1719 |
| HgaI | GACGC | 1 cut(s) | 561 |
| HhaI | GCGC | 4 cut(s) | 120, 444, 510, 1130 |
| Hin1I | GRCGYC | 2 cut(s) | 553, 2760 |
| Hin6I | GCGC | 4 cut(s) | 118, 442, 508, 1128 |
| HinP1I | GCGC | 4 cut(s) | 118, 442, 508, 1128 |
| HincII | GTYRAC | 2 cut(s) | 406, 1606 |
| HindII | GTYRAC | 2 cut(s) | 406, 1606 |
| HindIII | AAGCTT | 4 cut(s) | 290, 1790, 2048, 2449 |
| HpaI | GTTAAC | 1 cut(s) | 1606 |
| HpaII | CCGG | 5 cut(s) | 414, 420, 740, 857, 1719 |
| HphI | GGTGA | 5 cut(s) | 10, 73, 910, 1843, 2152 |
| Hpy166II | GTNNAC | 7 cut(s) | 31, 406, 584, 616, 1606, 1757, 2416 |
| Hpy188I | TCNGA | 9 cut(s) | 83, 197, 275, 636, 1275, 1296, 1418, 2345, 2484 |
| Hpy8I | GTNNAC | 7 cut(s) | 31, 406, 584, 616, 1606, 1757, 2416 |
| HpyCH4III | ACNGT | 5 cut(s) | 263, 667, 1256, 1738, 2349 |
| HpyCH4IV | ACGT | 3 cut(s) | 640, 2760, 2827 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 11, 514, 1118, 1127, 1136, 2047, 2090 |
| HpySE526I | ACGT | 3 cut(s) | 640, 2760, 2827 |
| Hsp92I | GRCGYC | 2 cut(s) | 553, 2760 |
| HspAI | GCGC | 4 cut(s) | 118, 442, 508, 1128 |
| KpnI | GGTACC | 1 cut(s) | 826 |
| Ksp22I | TGATCA | 3 cut(s) | 454, 708, 1213 |
| KspAI | GTTAAC | 1 cut(s) | 1606 |
| LmnI | GCTCC | 7 cut(s) | 115, 699, 1193, 1853, 2046, 2266, 2540 |
| Lsp1109I | GCAGC | 7 cut(s) | 795, 1159, 1387, 1460, 1556, 1599, 2619 |
| LweI | GCATC | 7 cut(s) | 129, 136, 211, 454, 856, 1308, 1497 |
| MabI | ACCWGGT | 3 cut(s) | 99, 2337, 2409 |
| MaeI | CTAG | 5 cut(s) | 50, 1136, 1527, 1871, 2555 |
| MaeII | ACGT | 3 cut(s) | 640, 2760, 2827 |
| MaeIII | GTNAC | 6 cut(s) | 16, 1250, 1409, 2158, 2307, 2524 |
| MfeI | CAATTG | 3 cut(s) | 498, 1113, 2036 |
| MflI | RGATCY | 3 cut(s) | 78, 622, 2377 |
| MhlI | GDGCHC | 4 cut(s) | 990, 1759, 2100, 2263 |
| MlsI | TGGCCA | 1 cut(s) | 1121 |
| MluNI | TGGCCA | 1 cut(s) | 1121 |
| MlyI | GAGTC | 4 cut(s) | 1052, 1982, 2069, 2371 |
| MmeI | TCCRAC | 1 cut(s) | 553 |
| Mox20I | TGGCCA | 1 cut(s) | 1121 |
| MroXI | GAANNNNTTC | 1 cut(s) | 641 |
| MscI | TGGCCA | 1 cut(s) | 1121 |
| MslI | CAYNNNNRTG | 4 cut(s) | 264, 1085, 1896, 2682 |
| Msp20I | TGGCCA | 1 cut(s) | 1121 |
| MspA1I | CMGCKG | 1 cut(s) | 2575 |
| MspCI | CTTAAG | 1 cut(s) | 133 |
| MspI | CCGG | 5 cut(s) | 414, 420, 740, 857, 1719 |
| MspR9I | CCNGG | 5 cut(s) | 101, 557, 1952, 2339, 2411 |
| MunI | CAATTG | 3 cut(s) | 498, 1113, 2036 |
| MvaI | CCWGG | 5 cut(s) | 101, 557, 1952, 2339, 2411 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 11, 514, 1118, 1127, 1136, 2047, 2090 |
| NcoI | CCATGG | 1 cut(s) | 768 |
| NdeI | CATATG | 1 cut(s) | 1329 |
| NmuCI | GTSAC | 2 cut(s) | 16, 2158 |
| NspI | RCATGY | 3 cut(s) | 656, 973, 1901 |
| NspV | TTCGAA | 1 cut(s) | 1588 |
| PagI | TCATGA | 1 cut(s) | 208 |
| PceI | AGGCCT | 1 cut(s) | 128 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 904 |
| PdmI | GAANNNNTTC | 1 cut(s) | 641 |
| PfeI | GAWTC | 6 cut(s) | 270, 795, 1004, 1441, 1679, 1927 |
| PflFI | GACNNNGTC | 1 cut(s) | 1941 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 572, 2687 |
| PinAI | ACCGGT | 2 cut(s) | 739, 1718 |
| PkrI | GCNGC | 7 cut(s) | 810, 1174, 1402, 1450, 1546, 1614, 2634 |
| PleI | GAGTC | 4 cut(s) | 1051, 1981, 2069, 2370 |
| PpsI | GAGTC | 4 cut(s) | 1051, 1981, 2069, 2370 |
| PpuMI | RGGWCCY | 3 cut(s) | 97, 2011, 2596 |
| PshBI | ATTAAT | 2 cut(s) | 1337, 2663 |
| Psp124BI | GAGCTC | 1 cut(s) | 2100 |
| Psp1406I | AACGTT | 1 cut(s) | 640 |
| Psp5II | RGGWCCY | 3 cut(s) | 97, 2011, 2596 |
| Psp6I | CCWGG | 5 cut(s) | 99, 555, 1950, 2337, 2409 |
| PspEI | GGTNACC | 1 cut(s) | 2158 |
| PspGI | CCWGG | 5 cut(s) | 99, 555, 1950, 2337, 2409 |
| PspPI | GGNCC | 4 cut(s) | 97, 1882, 2011, 2596 |
| PspPPI | RGGWCCY | 3 cut(s) | 97, 2011, 2596 |
| PstI | CTGCAG | 4 cut(s) | 813, 1405, 1617, 2020 |
| PsuI | RGATCY | 3 cut(s) | 78, 622, 2377 |
| PsyI | GACNNNGTC | 1 cut(s) | 1941 |
| RsaI | GTAC | 8 cut(s) | 651, 824, 1134, 1567, 1623, 1808, 2388, 2415 |
| RsaNI | GTAC | 8 cut(s) | 650, 823, 1133, 1566, 1622, 1807, 2387, 2414 |
| RseI | CAYNNNNRTG | 4 cut(s) | 264, 1085, 1896, 2682 |
| SacI | GAGCTC | 1 cut(s) | 2100 |
| SatI | GCNGC | 7 cut(s) | 809, 1173, 1401, 1449, 1545, 1613, 2633 |
| Sau96I | GGNCC | 4 cut(s) | 97, 1882, 2011, 2596 |
| SbfI | CCTGCAGG | 1 cut(s) | 2020 |
| SchI | GAGTC | 4 cut(s) | 1052, 1982, 2069, 2371 |
| ScrFI | CCNGG | 5 cut(s) | 101, 557, 1952, 2339, 2411 |
| SdaI | CCTGCAGG | 1 cut(s) | 2020 |
| SduI | GDGCHC | 4 cut(s) | 990, 1759, 2100, 2263 |
| SexAI | ACCWGGT | 3 cut(s) | 99, 2337, 2409 |
| SfaNI | GCATC | 7 cut(s) | 129, 136, 211, 454, 856, 1308, 1497 |
| SfcI | CTRYAG | 4 cut(s) | 809, 1401, 1613, 2016 |
| SfuI | TTCGAA | 1 cut(s) | 1588 |
| SinI | GGWCC | 4 cut(s) | 97, 1882, 2011, 2596 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 264, 1085, 1896, 2682 |
| SmlI | CTYRAG | 4 cut(s) | 133, 1802, 1816, 1931 |
| SmoI | CTYRAG | 4 cut(s) | 133, 1802, 1816, 1931 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 2020 |
| SseBI | AGGCCT | 1 cut(s) | 128 |
| SsiI | CCGC | 1 cut(s) | 2575 |
| SspMI | CTAG | 5 cut(s) | 50, 1136, 1527, 1871, 2555 |
| SstI | GAGCTC | 1 cut(s) | 2100 |
| StuI | AGGCCT | 1 cut(s) | 128 |
| StyD4I | CCNGG | 5 cut(s) | 99, 555, 1950, 2337, 2409 |
| StyI | CCWWGG | 3 cut(s) | 600, 768, 1878 |
| TaaI | ACNGT | 5 cut(s) | 263, 667, 1256, 1738, 2349 |
| TaiI | ACGT | 3 cut(s) | 643, 2763, 2830 |
| TaqI | TCGA | 8 cut(s) | 93, 228, 673, 907, 1075, 1460, 1588, 2646 |
| TatI | WGTACW | 2 cut(s) | 1132, 1621 |
| TfiI | GAWTC | 6 cut(s) | 270, 795, 1004, 1441, 1679, 1927 |
| TscAI | CASTG | 4 cut(s) | 553, 670, 1261, 2321 |
| TseFI | GTSAC | 2 cut(s) | 16, 2158 |
| TseI | GCWGC | 7 cut(s) | 808, 1172, 1400, 1448, 1544, 1612, 2632 |
| Tsp45I | GTSAC | 2 cut(s) | 16, 2158 |
| TspGWI | ACGGA | 2 cut(s) | 1221, 2164 |
| TspRI | CASTG | 4 cut(s) | 553, 670, 1261, 2321 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1941 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 572, 2687 |
| Vha464I | CTTAAG | 1 cut(s) | 133 |
| VneI | GTGCAC | 1 cut(s) | 1755 |
| VpaK11BI | GGWCC | 4 cut(s) | 97, 1882, 2011, 2596 |
| VspI | ATTAAT | 2 cut(s) | 1337, 2663 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 133, 377 |
| XapI | RAATTY | 5 cut(s) | 233, 330, 1057, 1839, 2639 |
| XbaI | TCTAGA | 2 cut(s) | 1526, 2554 |
| XceI | RCATGY | 3 cut(s) | 656, 973, 1901 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1005 |
| XmiI | GTMKAC | 1 cut(s) | 615 |
| XmnI | GAANNNNTTC | 1 cut(s) | 641 |
| XspI | CTAG | 5 cut(s) | 50, 1136, 1527, 1871, 2555 |
| ZraI | GACGTC | 1 cut(s) | 2761 |