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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 187 |
| AcuI | CTGAAG | 1 cut(s) | 30 |
| AgsI | TTSAA | 5 cut(s) | 56, 95, 101, 117, 146 |
| AluBI | AGCT | 2 cut(s) | 104, 128 |
| AluI | AGCT | 2 cut(s) | 104, 128 |
| AoxI | GGCC | 1 cut(s) | 29 |
| ApoI | RAATTY | 1 cut(s) | 187 |
| AsuHPI | GGTGA | 1 cut(s) | 69 |
| BfaI | CTAG | 3 cut(s) | 6, 107, 174 |
| BseGI | GGATG | 2 cut(s) | 34, 213 |
| BshFI | GGCC | 1 cut(s) | 31 |
| BsnI | GGCC | 1 cut(s) | 31 |
| BspANI | GGCC | 1 cut(s) | 31 |
| Bst4CI | ACNGT | 1 cut(s) | 181 |
| Bst6I | CTCTTC | 1 cut(s) | 96 |
| BstC8I | GCNNGC | 2 cut(s) | 122, 126 |
| BstF5I | GGATG | 2 cut(s) | 34, 213 |
| BsuRI | GGCC | 1 cut(s) | 31 |
| BtsCI | GGATG | 2 cut(s) | 34, 213 |
| Cac8I | GCNNGC | 2 cut(s) | 122, 126 |
| CviJI | RGCY | 7 cut(s) | 5, 31, 52, 104, 110, 120, 128 |
| CviKI_1 | RGCY | 7 cut(s) | 5, 31, 52, 104, 110, 120, 128 |
| Eam1104I | CTCTTC | 1 cut(s) | 96 |
| EarI | CTCTTC | 1 cut(s) | 96 |
| Eco57I | CTGAAG | 1 cut(s) | 30 |
| FaiI | YATR | 2 cut(s) | 134, 220 |
| FokI | GGATG | 1 cut(s) | 21 |
| FspBI | CTAG | 3 cut(s) | 6, 107, 174 |
| HaeIII | GGCC | 1 cut(s) | 31 |
| HincII | GTYRAC | 1 cut(s) | 154 |
| HindII | GTYRAC | 1 cut(s) | 154 |
| HinfI | GANTC | 1 cut(s) | 59 |
| HphI | GGTGA | 1 cut(s) | 69 |
| Hpy166II | GTNNAC | 1 cut(s) | 154 |
| Hpy8I | GTNNAC | 1 cut(s) | 154 |
| HpyAV | CCTTC | 1 cut(s) | 90 |
| HpyCH4III | ACNGT | 1 cut(s) | 181 |
| HpyCH4V | TGCA | 1 cut(s) | 71 |
| LmnI | GCTCC | 1 cut(s) | 133 |
| LpnPI | CCDG | 3 cut(s) | 33, 134, 189 |
| MaeI | CTAG | 3 cut(s) | 6, 107, 174 |
| MboII | GAAGA | 3 cut(s) | 25, 68, 83 |
| MfeI | CAATTG | 1 cut(s) | 21 |
| MluCI | AATT | 4 cut(s) | 21, 96, 112, 187 |
| MmeI | TCCRAC | 1 cut(s) | 62 |
| MnlI | CCTC | 4 cut(s) | 42, 73, 85, 99 |
| MunI | CAATTG | 1 cut(s) | 21 |
| NmeAIII | GCCGAG | 1 cut(s) | 7 |
| PfeI | GAWTC | 1 cut(s) | 59 |
| SetI | ASST | 6 cut(s) | 77, 82, 91, 106, 130, 166 |
| Sse9I | AATT | 4 cut(s) | 21, 96, 112, 187 |
| SspMI | CTAG | 3 cut(s) | 6, 107, 174 |
| TaaI | ACNGT | 1 cut(s) | 181 |
| TasI | AATT | 4 cut(s) | 21, 96, 112, 187 |
| TfiI | GAWTC | 1 cut(s) | 59 |
| XapI | RAATTY | 1 cut(s) | 187 |
| XspI | CTAG | 3 cut(s) | 6, 107, 174 |