Rh4CG062400

Histone-lysine n-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000700
Physical Location & Seq
Reverse (-)
11812720 .. 11814069
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG062400.1

Sequence Viewer

Length: 1296 bp
ATGGATCTCGACTGCACCAGAAAGAGAAGATCGTCACCACCAAGATGGTCTAAGCTTCCACCTGAACTATTAGGGTTCATCACGACAAAGCTTGGTTCGGTAGACACCATTCGCTGTAAGGCCGTTTGCACTTCTTGGAATCGAGCAGCCAAATCTTATATCTCTTCTCCATGTTACAAAGTCATGCATCAAACTCCGTTGCTCATGATTCCTAGCAGTAAAACGAAGGATCTCCATACTTGCCGCTTCTTCAGCCTTGCAGAGAACAATACTTACACTCTCAAAAACGTGTTTCGAGATTTTCCGGAGGCTTGGTGTGTGGGTTCATCACACGGATGGCTGGTGCTTATGGTTGGGACAGGAAAACTGTATCTTCTTAATCCCTTTACTGAAGAAAGGATTGAACTCCCATCCCTTTGGACTCTTAGTCACTTTGCCAAAATTAAAACTCGCCTGAACTCTTCCGGTGAGTTCCCGCGGAGTACCATATCACTGGATGGTTATATTATGGGTAAAGTTGTCACTTCTTGTAACCCGTCTTGCAACAAGGACTTTGCTGTTGTGATAACTCACTGCTTGAGAGGAAGACTCGCATTTTGTAAGCATGGGGACGACAGATGTACGTGGATTGACCTAGGAGTCGAGTCCGATTACTATAGTGATATTATCTTCCACAATGGCCATTTATATACATTGACATCCGGCGGTTCTGTTGGAGTTTGGGACATGACGAAATCTTTTCCCACCAAAATTTTTGATCTTCACCCTTTTGCAGACCAGTCGGAGATAAATAAACACTTTCCCTTTGCCAAATTTGATGTTAAGCATTATTTGGTGGAGTCGTCGGGCGACCTTTTATTTGTTTGGCGATTTATAGGGAAGATTATTGAAGGCGAAAAGCTCCCATATAGAACCTTGCACTTTCATATCTTTAGGCTGAGTTCCACTAGCAAAGAGTGGGAGAAAGTGGAATATTTGCATGACCGGGCTCTGTTTTTGGGTTGCAGTCATTCAATGTCAGTATCCTCCCGAGTCTTTCCTGAATGTCAAGAAAACTCGATCTACTTTACAGATGATCATTCTTTTAAGATGACTTTTAAGTATTTTGGCCGCCTCGGTCTTGATCTAGGAGTATACAATTTAAAAGATAAGGTTGTCGAGCCATTCGCAGGTCACCAATTTTTTGATAAGTGGACAATTTATGATCCACTTCCCTTCTGGATTTTTCCCAATCCTTGGCCTTCGACAGTGAGCTACAAAGCAGCATCACCCTCGCTATCAGGACCACAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
Pre-SET SET WIYLD

Protein Analysis

431

Amino Acids

49.48

Weight (kDa)

8.74

Isoelectric Point (pI)

43.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 83 - 380 1.8e-61 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000700)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27550
fragaria_vesca FvH4_3g40651 FvH4_3g40751 FvH4_3g43771 FvH4_4g04480 FvH4_4g26310 FvH4_4g26320 FvH4_4g30091 FvH4_6g03860 FvH4_7g10560
malus_domestica MD13G1117600.v1.1 MD13G1223500.v1.1 MD16G1228400.v1.1
prunus_persica Prupe.1G050400_v2.0.a1 Prupe.1G050500_v2.0.a1 Prupe.1G227500_v2.0.a1 Prupe.1G227900_v2.0.a1 Prupe.1G228000_v2.0.a1 Prupe.1G228100_v2.0.a1 Prupe.6G285500_v2.0.a1
pyrus_communis pycom13g10170 pycom16g10020
rosa_chinensis RchiOBHm_Chr1g0339581 RchiOBHm_Chr3g0452431 RchiOBHm_Chr4g0395201 RchiOBHm_Chr4g0430571 RchiOBHm_Chr4g0433901
rosa_laevigata RLG00000006969 RLG00000009529 RLG00000009536 RLG00000009538 RLG00000009542 RLG00000009640 RLG00000025603 RLG00000029238 RLG00000036257
rosa_multiflora Rmu_co8444313.1_g000001 Rmu_sc0000352.1_g000004 Rmu_sc0002149.1_g000002 Rmu_sc0006211.1_g000002 Rmu_sc0011680.1_g000008 Rmu_sc0011680.1_g000009 Rmu_ssc0000387.1_g000032
rosa_roxburghii Rroxscaffold_1G00007830 Rroxscaffold_1G00009290 Rroxscaffold_4G00313550 Rroxscaffold_5G00340130 Rroxscaffold_5G00375300 Rroxscaffold_6G00429450
rosa_rugosa Rorug01G0140200.1 Rorug01G0140500.1 Rorug01G0179500 Rorug02G0640800 Rorug03G0359000.1 Rorug04G0244300 Rorug05G0415200
rosa_samantha Rh1AG158200 Rh3AG042600 Rh3BG044200 Rh4CG062400 Rh4CG074400 Rh4CG324100 Rh4CG324200 Rh4CG350200 Rh5AG471400 Rh5BG491100
rosa_wichuraiana Rw1G013110 Rw3G003280 Rw4G004620 Rw4G005740 Rw4G005750 Rw4G028350 Rw5G043800 Rw5G044960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 638
Acc36I ACCTGC 1 cut(s) 1160
AccB7I CCANNNNNTGG 1 cut(s) 1236
AccI GTMKAC 2 cut(s) 102, 1134
AccII CGCG 1 cut(s) 478
AccIII TCCGGA 1 cut(s) 304
AciI CCGC 5 cut(s) 244, 476, 478, 705, 1111
AclWI GGATC 3 cut(s) 12, 237, 1199
AcoI YGGCCR 2 cut(s) 679, 1108
AcsI RAATTY 2 cut(s) 750, 812
AcuI CTGAAG 2 cut(s) 235, 411
AfaI GTAC 2 cut(s) 484, 622
AfiI CCNNNNNNNGG 2 cut(s) 1169, 1236
AflIII ACRYGT 1 cut(s) 288
AgsI TTSAA 3 cut(s) 404, 890, 1014
AhdI GACNNNNNGTC 1 cut(s) 426
AluBI AGCT 4 cut(s) 55, 91, 901, 1254
AluI AGCT 4 cut(s) 55, 91, 901, 1254
AlwI GGATC 3 cut(s) 12, 237, 1199
Ama87I CYCGRG 1 cut(s) 1029
Aor13HI TCCGGA 1 cut(s) 304
AoxI GGCC 4 cut(s) 120, 679, 1108, 1238
ApeKI GCWGC 2 cut(s) 146, 1262
ApoI RAATTY 2 cut(s) 750, 812
ArsI GACNNNNNNTTYG 4 cut(s) 579, 611, 842, 874
AspA2I CCTAGG 1 cut(s) 634
AspS9I GGNCC 1 cut(s) 1283
AsuC2I CCSGG 1 cut(s) 986
AsuHPI GGTGA 5 cut(s) 27, 479, 755, 1166, 1260
AvaI CYCGRG 1 cut(s) 1029
AvaII GGWCC 1 cut(s) 1283
AvrII CCTAGG 1 cut(s) 634
BalI TGGCCA 1 cut(s) 681
BanII GRGCYC 1 cut(s) 991
BbsI GAAGAC 1 cut(s) 592
BbvI GCAGC 2 cut(s) 158, 1274
BccI CCATC 4 cut(s) 39, 330, 418, 491
BceAI ACGGC 1 cut(s) 107
BcgI CGANNNNNNTGC 4 cut(s) 762, 796, 1254, 1288
BciVI GTATCC 1 cut(s) 1033
BclI TGATCA 1 cut(s) 1075
BcnI CCSGG 1 cut(s) 986
BfaI CTAG 4 cut(s) 213, 635, 948, 1127
BfmI CTRYAG 1 cut(s) 655
BfuAI ACCTGC 1 cut(s) 1160
BfuI GTATCC 1 cut(s) 1033
BisI GCNGC 4 cut(s) 147, 244, 1111, 1263
BlnI CCTAGG 1 cut(s) 634
BlsI GCNGC 4 cut(s) 148, 245, 1112, 1264
Bme1390I CCNGG 1 cut(s) 986
Bme18I GGWCC 1 cut(s) 1283
BmeRI GACNNNNNGTC 1 cut(s) 426
BmeT110I CYCGRG 1 cut(s) 1029
BmgT120I GGNCC 1 cut(s) 1283
BmrFI CCNGG 1 cut(s) 986
BmsI GCATC 2 cut(s) 196, 1274
BpiI GAAGAC 1 cut(s) 592
BplI GAGNNNNNCTC 2 cut(s) 573, 605
BpuEI CTTGAG 1 cut(s) 598
BpuMI CCSGG 1 cut(s) 986
BsaAI YACGTR 1 cut(s) 624
BsaJI CCNNGG 4 cut(s) 476, 634, 1114, 1235
BsaWI WCCGGW 2 cut(s) 304, 464
BsaXI ACNNNNNCTCC 2 cut(s) 299, 329
Bsc4I CCNNNNNNNGG 2 cut(s) 1169, 1236
Bse1I ACTGG 2 cut(s) 498, 778
BseAI TCCGGA 1 cut(s) 304
BseDI CCNNGG 4 cut(s) 476, 634, 1114, 1235
BseGI GGATG 4 cut(s) 341, 410, 502, 698
BseLI CCNNNNNNNGG 2 cut(s) 1169, 1236
BseMII CTCAG 1 cut(s) 929
BseNI ACTGG 2 cut(s) 498, 778
BseXI GCAGC 2 cut(s) 158, 1274
Bsh1236I CGCG 1 cut(s) 478
BshFI GGCC 4 cut(s) 122, 681, 1110, 1240
BsiHKCI CYCGRG 1 cut(s) 1029
BsiSI CCGG 4 cut(s) 305, 465, 702, 985
BslFI GGGAC 3 cut(s) 370, 623, 737
BslI CCNNNNNNNGG 2 cut(s) 1169, 1236
BsmFI GGGAC 3 cut(s) 370, 623, 737
BsnI GGCC 4 cut(s) 122, 681, 1110, 1240
BsoBI CYCGRG 1 cut(s) 1029
Bsp1286I GDGCHC 1 cut(s) 991
Bsp13I TCCGGA 1 cut(s) 304
Bsp143I GATC 8 cut(s) 4, 29, 229, 757, 1059, 1075, 1123, 1204
BspACI CCGC 5 cut(s) 244, 476, 478, 705, 1111
BspANI GGCC 4 cut(s) 122, 681, 1110, 1240
BspCNI CTCAG 1 cut(s) 930
BspEI TCCGGA 1 cut(s) 304
BspFNI CGCG 1 cut(s) 478
BspHI TCATGA 1 cut(s) 204
BspMI ACCTGC 1 cut(s) 1160
BspPI GGATC 3 cut(s) 12, 237, 1199
BsrI ACTGG 2 cut(s) 498, 778
BssECI CCNNGG 4 cut(s) 476, 634, 1114, 1235
BssMI GATC 8 cut(s) 4, 29, 229, 757, 1059, 1075, 1123, 1204
BssNAI GTATAC 1 cut(s) 1135
BssT1I CCWWGG 2 cut(s) 634, 1235
Bst1107I GTATAC 1 cut(s) 1135
Bst4CI ACNGT 2 cut(s) 369, 1249
Bst6I CTCTTC 2 cut(s) 169, 466
BstBAI YACGTR 1 cut(s) 624
BstDEI CTNAG 3 cut(s) 51, 425, 938
BstDSI CCRYGG 1 cut(s) 476
BstEII GGTNACC 1 cut(s) 1172
BstF5I GGATG 4 cut(s) 341, 410, 502, 698
BstFNI CGCG 1 cut(s) 478
BstKTI GATC 8 cut(s) 7, 32, 232, 760, 1062, 1078, 1126, 1207
BstMBI GATC 8 cut(s) 4, 29, 229, 757, 1059, 1075, 1123, 1204
BstMWI GCNNNNNNNGC 1 cut(s) 252
BstPI GGTNACC 1 cut(s) 1172
BstSCI CCNGG 1 cut(s) 984
BstSFI CTRYAG 1 cut(s) 655
BstUI CGCG 1 cut(s) 478
BstV1I GCAGC 2 cut(s) 158, 1274
BstV2I GAAGAC 1 cut(s) 592
BstX2I RGATCY 2 cut(s) 4, 229
BstXI CCANNNNNNTGG 3 cut(s) 45, 417, 493
BstYI RGATCY 2 cut(s) 4, 229
BstZ17I GTATAC 1 cut(s) 1135
BsuI GTATCC 1 cut(s) 1033
BsuRI GGCC 4 cut(s) 122, 681, 1110, 1240
BtgI CCRYGG 1 cut(s) 476
BtsCI GGATG 4 cut(s) 341, 410, 502, 698
BtsI GCAGTG 1 cut(s) 571
BtsIMutI CAGTG 3 cut(s) 491, 571, 1254
BveI ACCTGC 1 cut(s) 1160
CciI TCATGA 1 cut(s) 204
Cfr13I GGNCC 1 cut(s) 1283
Cfr42I CCGCGG 1 cut(s) 479
Csp6I GTAC 2 cut(s) 483, 621
CviAII CATG 6 cut(s) 171, 184, 205, 605, 727, 980
CviQI GTAC 2 cut(s) 483, 621
DdeI CTNAG 3 cut(s) 51, 425, 938
DpnI GATC 8 cut(s) 6, 31, 231, 759, 1061, 1077, 1125, 1206
DpnII GATC 8 cut(s) 4, 29, 229, 757, 1059, 1075, 1123, 1204
DraI TTTAAA 1 cut(s) 1143
DrdI GACNNNNNNGTC 1 cut(s) 638
DriI GACNNNNNGTC 1 cut(s) 426
DseDI GACNNNNNNGTC 1 cut(s) 638
EaeI YGGCCR 2 cut(s) 679, 1108
Eam1104I CTCTTC 2 cut(s) 169, 466
Eam1105I GACNNNNNGTC 1 cut(s) 426
EarI CTCTTC 2 cut(s) 169, 466
Eco130I CCWWGG 2 cut(s) 634, 1235
Eco24I GRGCYC 1 cut(s) 991
Eco47I GGWCC 1 cut(s) 1283
Eco57I CTGAAG 2 cut(s) 235, 411
Eco88I CYCGRG 1 cut(s) 1029
Eco91I GGTNACC 1 cut(s) 1172
EcoO65I GGTNACC 1 cut(s) 1172
EcoT14I CCWWGG 2 cut(s) 634, 1235
EcoT22I ATGCAT 1 cut(s) 189
EcoT38I GRGCYC 1 cut(s) 991
ErhI CCWWGG 2 cut(s) 634, 1235
FaeI CATG 6 cut(s) 174, 187, 208, 608, 730, 983
FaqI GGGAC 3 cut(s) 370, 623, 737
FatI CATG 6 cut(s) 170, 183, 204, 604, 726, 979
FauI CCCGC 1 cut(s) 483
FbaI TGATCA 1 cut(s) 1075
FblI GTMKAC 2 cut(s) 102, 1134
Fnu4HI GCNGC 4 cut(s) 147, 244, 1111, 1263
FokI GGATG 4 cut(s) 348, 397, 509, 685
FriOI GRGCYC 1 cut(s) 991
Fsp4HI GCNGC 4 cut(s) 147, 244, 1111, 1263
FspBI CTAG 4 cut(s) 213, 635, 948, 1127
GluI GCNGC 4 cut(s) 147, 244, 1111, 1263
HaeIII GGCC 4 cut(s) 122, 681, 1110, 1240
HapII CCGG 4 cut(s) 305, 465, 702, 985
Hin1II CATG 6 cut(s) 174, 187, 208, 608, 730, 983
HindIII AAGCTT 2 cut(s) 53, 89
HinfI GANTC 8 cut(s) 139, 208, 421, 588, 639, 644, 839, 1032
HpaII CCGG 4 cut(s) 305, 465, 702, 985
HphI GGTGA 5 cut(s) 27, 479, 755, 1166, 1260
Hpy166II GTNNAC 3 cut(s) 103, 1135, 1194
Hpy188I TCNGA 2 cut(s) 649, 784
Hpy8I GTNNAC 3 cut(s) 103, 1135, 1194
Hpy99I CGWCG 1 cut(s) 847
HpyAV CCTTC 4 cut(s) 220, 884, 1225, 1251
HpyCH4III ACNGT 2 cut(s) 369, 1249
HpyCH4IV ACGT 2 cut(s) 288, 623
HpyCH4V TGCA 9 cut(s) 15, 129, 187, 260, 543, 773, 919, 979, 1005
HpyF10VI GCNNNNNNNGC 1 cut(s) 252
HpyF3I CTNAG 3 cut(s) 51, 425, 938
HpySE526I ACGT 2 cut(s) 288, 623
Hsp92II CATG 6 cut(s) 174, 187, 208, 608, 730, 983
Kpn2I TCCGGA 1 cut(s) 304
Ksp22I TGATCA 1 cut(s) 1075
KspI CCGCGG 1 cut(s) 479
Kzo9I GATC 8 cut(s) 4, 29, 229, 757, 1059, 1075, 1123, 1204
LmnI GCTCC 1 cut(s) 906
Lsp1109I GCAGC 2 cut(s) 158, 1274
LweI GCATC 2 cut(s) 196, 1274
MaeI CTAG 4 cut(s) 213, 635, 948, 1127
MaeII ACGT 2 cut(s) 288, 623
MaeIII GTNAC 6 cut(s) 33, 173, 428, 520, 530, 1172
MalI GATC 8 cut(s) 6, 31, 231, 759, 1061, 1077, 1125, 1206
MboI GATC 8 cut(s) 4, 29, 229, 757, 1059, 1075, 1123, 1204
MflI RGATCY 2 cut(s) 4, 229
MhlI GDGCHC 1 cut(s) 991
MlsI TGGCCA 1 cut(s) 681
MluCI AATT 6 cut(s) 441, 750, 812, 1138, 1178, 1197
MluNI TGGCCA 1 cut(s) 681
MlyI GAGTC 6 cut(s) 415, 582, 648, 653, 848, 1041
MmeI TCCRAC 2 cut(s) 694, 762
MnlI CCTC 5 cut(s) 301, 575, 1036, 1124, 1282
Mox20I TGGCCA 1 cut(s) 681
Mph1103I ATGCAT 1 cut(s) 189
MroI TCCGGA 1 cut(s) 304
MscI TGGCCA 1 cut(s) 681
MseI TTAA 6 cut(s) 378, 444, 822, 1086, 1098, 1142
MslI CAYNNNNRTG 2 cut(s) 43, 334
Msp20I TGGCCA 1 cut(s) 681
MspA1I CMGCKG 1 cut(s) 478
MspI CCGG 4 cut(s) 305, 465, 702, 985
MspR9I CCNGG 1 cut(s) 986
MvnI CGCG 1 cut(s) 478
MwoI GCNNNNNNNGC 1 cut(s) 252
NciI CCSGG 1 cut(s) 986
NdeII GATC 8 cut(s) 4, 29, 229, 757, 1059, 1075, 1123, 1204
NlaIII CATG 6 cut(s) 174, 187, 208, 608, 730, 983
NmuCI GTSAC 4 cut(s) 33, 428, 520, 1172
NsiI ATGCAT 1 cut(s) 189
PagI TCATGA 1 cut(s) 204
PfeI GAWTC 2 cut(s) 139, 208
PflMI CCANNNNNTGG 1 cut(s) 1236
PkrI GCNGC 4 cut(s) 148, 245, 1112, 1264
PleI GAGTC 6 cut(s) 415, 582, 647, 652, 847, 1040
PpsI GAGTC 6 cut(s) 415, 582, 647, 652, 847, 1040
Ppu21I YACGTR 1 cut(s) 624
PspEI GGTNACC 1 cut(s) 1172
PspPI GGNCC 1 cut(s) 1283
PsrI GAACNNNNNNTAC 2 cut(s) 257, 289
PsuI RGATCY 2 cut(s) 4, 229
RsaI GTAC 2 cut(s) 484, 622
RsaNI GTAC 2 cut(s) 483, 621
RseI CAYNNNNRTG 2 cut(s) 43, 334
SacII CCGCGG 1 cut(s) 479
SaqAI TTAA 6 cut(s) 378, 444, 822, 1086, 1098, 1142
SatI GCNGC 4 cut(s) 147, 244, 1111, 1263
Sau3AI GATC 8 cut(s) 4, 29, 229, 757, 1059, 1075, 1123, 1204
Sau96I GGNCC 1 cut(s) 1283
SchI GAGTC 6 cut(s) 415, 582, 648, 653, 848, 1041
ScrFI CCNGG 1 cut(s) 986
SduI GDGCHC 1 cut(s) 991
SfaNI GCATC 2 cut(s) 196, 1274
SfcI CTRYAG 1 cut(s) 655
Sfr303I CCGCGG 1 cut(s) 479
SgrBI CCGCGG 1 cut(s) 479
SinI GGWCC 1 cut(s) 1283
SmiMI CAYNNNNRTG 2 cut(s) 43, 334
SmlI CTYRAG 1 cut(s) 577
SmoI CTYRAG 1 cut(s) 577
Sse9I AATT 6 cut(s) 441, 750, 812, 1138, 1178, 1197
SsiI CCGC 5 cut(s) 244, 476, 478, 705, 1111
SspI AATATT 1 cut(s) 974
SspMI CTAG 4 cut(s) 213, 635, 948, 1127
StyD4I CCNGG 1 cut(s) 984
StyI CCWWGG 2 cut(s) 634, 1235
TaaI ACNGT 2 cut(s) 369, 1249
TaiI ACGT 2 cut(s) 291, 626
TaqI TCGA 7 cut(s) 9, 142, 295, 642, 1058, 1158, 1244
TaqII GACCGA 1 cut(s) 1106
TasI AATT 6 cut(s) 441, 750, 812, 1138, 1178, 1197
TauI GCSGC 2 cut(s) 246, 1113
TfiI GAWTC 2 cut(s) 139, 208
Tru1I TTAA 6 cut(s) 378, 444, 822, 1086, 1098, 1142
Tru9I TTAA 6 cut(s) 378, 444, 822, 1086, 1098, 1142
TscAI CASTG 3 cut(s) 498, 578, 1254
TseFI GTSAC 4 cut(s) 33, 428, 520, 1172
TseI GCWGC 2 cut(s) 146, 1262
Tsp45I GTSAC 4 cut(s) 33, 428, 520, 1172
TspDTI ATGAA 3 cut(s) 67, 315, 914
TspGWI ACGGA 2 cut(s) 186, 348
TspRI CASTG 3 cut(s) 498, 578, 1254
Van91I CCANNNNNTGG 1 cut(s) 1236
VpaK11BI GGWCC 1 cut(s) 1283
XapI RAATTY 2 cut(s) 750, 812
XcmI CCANNNNNNNNNTGG 1 cut(s) 1215
XmaJI CCTAGG 1 cut(s) 634
XmiI GTMKAC 2 cut(s) 102, 1134
XspI CTAG 4 cut(s) 213, 635, 948, 1127
Zsp2I ATGCAT 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.