AT5G32450

RNA recognition motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000747
Physical Location & Seq
Forward (+)
12079528 .. 12081647
2120 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G32450.1

Sequence Viewer

Length: 804 bp
ATGCAGACGACGAGAAGTGTTCAAGTAAATAATGTCTCAGATCTAGCCACAGAGAGAGAGATTCACGAGTTTTTCTCCTTCTCAGGCGACATAGAACACATTGAAATCCAAAAAGAATTTGGACAGTCTCGAATCGCATTTGTTACATTTACTGATCCCAAAGCCCTCGAAATTGCCTTGTTATTATCGGGTGCGACGATTGTGGACCAGATTGTTACAATAACACGAGCTGAAAACTATGTTCAGAGGCGTGAGACACAGGAAGTAAGAATGTTGGACAACGCGATGCCTCTTGGTCTTCAAGAGAGTACTACACAGACTAAAACGAACATGGATGGTAATAGTAGAGCATACGTTAGCAAAGCACAAGATGTGGTGGCAACGGTTTTGGCAAAAGGGTCTGCTCTTGGACAAGATGCAGTGAATAAAGCCAAGGCTTTCGACGAAAAGCACCAATTAAGAGCCAATGCTTCTGCTAAAGTTTCATCTTTTGACAAGCGAGTTGGTCTTACTGAGAAGTTATCTGTTGGTATTTCTGCTGTTAATGAGAAAGTTAAATCTGTTGATCAAAAGCTTCAAGTCTCCGATAAAACTATGGCAGCAATATTTGCAGCCGAGAGGAAGTTAAACGACACGGGTTCAGCTGTCAAATCAAGCAGGTACGTGACTGCTGGAGCTGCTTGGTTTAGTGGCGCTTTTAGTAAAGTGGCGAGAGTAGGTCAGGTTGCTGGCTCAAAAACCAAAGAGAAGTTCAATCTAGCCGTCTCAAATATGAGTTCCAAGGATACACCGATTGCTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

267

Amino Acids

28.97

Weight (kDa)

9.28

Isoelectric Point (pI)

30.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 9 - 67 4.9e-06 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 648
AccII CGCG 1 cut(s) 284
AclWI GGATC 1 cut(s) 149
AcsI RAATTY 1 cut(s) 116
AfaI GTAC 2 cut(s) 310, 662
AgsI TTSAA 5 cut(s) 23, 104, 302, 578, 754
AjuI GAANNNNNNNTTGG 2 cut(s) 734, 766
AluBI AGCT 4 cut(s) 230, 574, 644, 677
AluI AGCT 4 cut(s) 230, 574, 644, 677
Alw26I GTCTC 5 cut(s) 40, 132, 248, 586, 769
AlwI GGATC 1 cut(s) 149
ApeKI GCWGC 3 cut(s) 599, 611, 677
ApoI RAATTY 1 cut(s) 116
AspLEI GCGC 1 cut(s) 695
AspS9I GGNCC 1 cut(s) 205
AvaII GGWCC 1 cut(s) 205
BauI CACGAG 2 cut(s) 65, 225
BbsI GAAGAC 1 cut(s) 290
BbvI GCAGC 3 cut(s) 611, 623, 664
BccI CCATC 1 cut(s) 329
BceAI ACGGC 1 cut(s) 746
BciVI GTATCC 1 cut(s) 778
BclI TGATCA 1 cut(s) 565
BcoDI GTCTC 5 cut(s) 40, 132, 248, 586, 769
BfaI CTAG 2 cut(s) 44, 758
BfoI RGCGCY 1 cut(s) 696
BfuAI ACCTGC 1 cut(s) 648
BfuI GTATCC 1 cut(s) 778
BglII AGATCT 1 cut(s) 40
BisI GCNGC 3 cut(s) 600, 612, 678
BlsI GCNGC 3 cut(s) 601, 613, 679
BmcAI AGTACT 1 cut(s) 310
Bme18I GGWCC 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 205
BmsI GCATC 2 cut(s) 276, 406
BpiI GAAGAC 1 cut(s) 290
BplI GAGNNNNNCTC 2 cut(s) 59, 91
BpmI CTGGAG 1 cut(s) 693
BsaAI YACGTR 1 cut(s) 664
BsaJI CCNNGG 2 cut(s) 432, 780
BseDI CCNNGG 2 cut(s) 432, 780
BseGI GGATG 1 cut(s) 340
BseMII CTCAG 3 cut(s) 51, 96, 504
BseXI GCAGC 3 cut(s) 611, 623, 664
Bsh1236I CGCG 1 cut(s) 284
BsmAI GTCTC 5 cut(s) 40, 132, 248, 586, 769
BsmBI CGTCTC 1 cut(s) 769
Bsp143I GATC 3 cut(s) 40, 154, 565
BspCNI CTCAG 3 cut(s) 50, 95, 505
BspFNI CGCG 1 cut(s) 284
BspMI ACCTGC 1 cut(s) 648
BspPI GGATC 1 cut(s) 149
BssECI CCNNGG 2 cut(s) 432, 780
BssMI GATC 3 cut(s) 40, 154, 565
BssSI CACGAG 2 cut(s) 65, 225
BssT1I CCWWGG 2 cut(s) 432, 780
Bst2BI CACGAG 2 cut(s) 65, 225
Bst4CI ACNGT 2 cut(s) 126, 385
BstAPI GCANNNNNTGC 1 cut(s) 608
BstBAI YACGTR 1 cut(s) 664
BstC8I GCNNGC 1 cut(s) 730
BstDEI CTNAG 3 cut(s) 37, 82, 513
BstF5I GGATG 1 cut(s) 340
BstFNI CGCG 1 cut(s) 284
BstH2I RGCGCY 1 cut(s) 696
BstHHI GCGC 1 cut(s) 695
BstKTI GATC 3 cut(s) 43, 157, 568
BstMAI GTCTC 5 cut(s) 40, 132, 248, 586, 769
BstMBI GATC 3 cut(s) 40, 154, 565
BstMWI GCNNNNNNNGC 2 cut(s) 608, 677
BstUI CGCG 1 cut(s) 284
BstV1I GCAGC 3 cut(s) 611, 623, 664
BstV2I GAAGAC 1 cut(s) 290
BstX2I RGATCY 1 cut(s) 40
BstYI RGATCY 1 cut(s) 40
BsuI GTATCC 1 cut(s) 778
BtgZI GCGATG 1 cut(s) 299
BtsCI GGATG 1 cut(s) 340
BtsI GCAGTG 1 cut(s) 426
BtsIMutI CAGTG 1 cut(s) 426
BveI ACCTGC 1 cut(s) 648
Cac8I GCNNGC 1 cut(s) 730
CfoI GCGC 1 cut(s) 695
Cfr13I GGNCC 1 cut(s) 205
Csp6I GTAC 2 cut(s) 309, 661
CviAII CATG 1 cut(s) 331
CviQI GTAC 2 cut(s) 309, 661
DdeI CTNAG 3 cut(s) 37, 82, 513
DpnI GATC 3 cut(s) 42, 156, 567
DpnII GATC 3 cut(s) 40, 154, 565
Eco130I CCWWGG 2 cut(s) 432, 780
Eco47I GGWCC 1 cut(s) 205
EcoT14I CCWWGG 2 cut(s) 432, 780
ErhI CCWWGG 2 cut(s) 432, 780
Esp3I CGTCTC 1 cut(s) 769
FaeI CATG 1 cut(s) 334
FaiI YATR 7 cut(s) 92, 240, 332, 352, 596, 773, 802
FatI CATG 1 cut(s) 330
FbaI TGATCA 1 cut(s) 565
Fnu4HI GCNGC 3 cut(s) 600, 612, 678
FokI GGATG 1 cut(s) 347
Fsp4HI GCNGC 3 cut(s) 600, 612, 678
FspBI CTAG 2 cut(s) 44, 758
GlaI GCGC 1 cut(s) 694
GluI GCNGC 3 cut(s) 600, 612, 678
GsuI CTGGAG 1 cut(s) 693
HaeII RGCGCY 1 cut(s) 696
HhaI GCGC 1 cut(s) 695
Hin1II CATG 1 cut(s) 334
Hin6I GCGC 1 cut(s) 693
HinP1I GCGC 1 cut(s) 693
HindIII AAGCTT 1 cut(s) 572
HinfI GANTC 2 cut(s) 61, 132
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 3 cut(s) 40, 246, 586
Hpy188III TCNNGA 3 cut(s) 65, 129, 302
Hpy8I GTNNAC 1 cut(s) 205
Hpy99I CGWCG 3 cut(s) 13, 199, 446
HpyAV CCTTC 1 cut(s) 88
HpyCH4III ACNGT 2 cut(s) 126, 385
HpyCH4IV ACGT 2 cut(s) 354, 663
HpyCH4V TGCA 3 cut(s) 4, 419, 611
HpyF10VI GCNNNNNNNGC 2 cut(s) 608, 677
HpyF3I CTNAG 3 cut(s) 37, 82, 513
HpySE526I ACGT 2 cut(s) 354, 663
Hsp92II CATG 1 cut(s) 334
HspAI GCGC 1 cut(s) 693
Ksp22I TGATCA 1 cut(s) 565
Kzo9I GATC 3 cut(s) 40, 154, 565
LmnI GCTCC 1 cut(s) 674
LpnPI CCDG 7 cut(s) 69, 221, 245, 643, 657, 707, 714
Lsp1109I GCAGC 3 cut(s) 611, 623, 664
LweI GCATC 2 cut(s) 276, 406
MaeI CTAG 2 cut(s) 44, 758
MaeII ACGT 2 cut(s) 354, 663
MaeIII GTNAC 3 cut(s) 142, 214, 664
MalI GATC 3 cut(s) 42, 156, 567
MboI GATC 3 cut(s) 40, 154, 565
MboII GAAGA 1 cut(s) 290
MflI RGATCY 1 cut(s) 40
MluCI AATT 3 cut(s) 116, 171, 455
MmeI TCCRAC 1 cut(s) 255
MnlI CCTC 4 cut(s) 176, 240, 300, 612
MseI TTAA 4 cut(s) 458, 543, 555, 626
MspA1I CMGCKG 1 cut(s) 644
MvnI CGCG 1 cut(s) 284
MwoI GCNNNNNNNGC 2 cut(s) 608, 677
NdeII GATC 3 cut(s) 40, 154, 565
NlaIII CATG 1 cut(s) 334
NmeAIII GCCGAG 1 cut(s) 640
NmuCI GTSAC 1 cut(s) 664
PcsI WCGNNNNNNNCGW 1 cut(s) 194
PfeI GAWTC 2 cut(s) 61, 132
PkrI GCNGC 3 cut(s) 601, 613, 679
Ppu21I YACGTR 1 cut(s) 664
PspPI GGNCC 1 cut(s) 205
PsuI RGATCY 1 cut(s) 40
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 2 cut(s) 310, 662
RsaNI GTAC 2 cut(s) 309, 661
SaqAI TTAA 4 cut(s) 458, 543, 555, 626
SatI GCNGC 3 cut(s) 600, 612, 678
Sau3AI GATC 3 cut(s) 40, 154, 565
Sau96I GGNCC 1 cut(s) 205
ScaI AGTACT 1 cut(s) 310
SetI ASST 9 cut(s) 232, 357, 576, 646, 662, 666, 679, 721, 726
SfaNI GCATC 2 cut(s) 276, 406
SinI GGWCC 1 cut(s) 205
Sse9I AATT 3 cut(s) 116, 171, 455
SspI AATATT 1 cut(s) 606
SspMI CTAG 2 cut(s) 44, 758
StyI CCWWGG 2 cut(s) 432, 780
TaaI ACNGT 2 cut(s) 126, 385
TaiI ACGT 2 cut(s) 357, 666
TaqI TCGA 3 cut(s) 130, 168, 441
TasI AATT 3 cut(s) 116, 171, 455
TatI WGTACW 1 cut(s) 308
TfiI GAWTC 2 cut(s) 61, 132
Tru1I TTAA 4 cut(s) 458, 543, 555, 626
Tru9I TTAA 4 cut(s) 458, 543, 555, 626
TscAI CASTG 1 cut(s) 426
TseFI GTSAC 1 cut(s) 664
TseI GCWGC 3 cut(s) 599, 611, 677
Tsp45I GTSAC 1 cut(s) 664
TspDTI ATGAA 1 cut(s) 474
TspRI CASTG 1 cut(s) 426
VpaK11BI GGWCC 1 cut(s) 205
XapI RAATTY 1 cut(s) 116
XcmI CCANNNNNNNNNTGG 1 cut(s) 116
XspI CTAG 2 cut(s) 44, 758
ZrmI AGTACT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.