FvH4_3g22070

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001294
Physical Location & Seq
Forward (+)
15117191 .. 15118409
1219 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g22070.t1

Sequence Viewer

Length: 936 bp
ATGGGAGCCATGAAGAGAAAGGCGGCGGAAGTGAGTTTAGTTTCAGGGGAGGAGAAATGGAGAAGAAAGGTCGGAGCCCTGAAGAGTAAGCCGAAACCGGTGAGCTTTGGTTCCGGAGAAAAGTGGGTCCGAGGAAAAAAAATCGGAGAAGGAGGCTTCGGTTCCGTGTTTGTTGCATTCTCCAAGAGTCGCTATATGCCGAGAGCCATGGCGATAAAATCGGCGGAAATGAATGAATCCGATTCCATCCAGTACGAGCTTCAAGTGTACCAGGAATTGGATGGTGATTCCTGCCCTTTCATCATCGAGCACTATGGGGAAGAGGTTACTCTGAGTAGCAAAGGTGAAAAATTCTACAACTTGGCCTTGGAGATTGCTTGGGGAAGCCTTGCCGAGTGGATCAAGGTTTCCGGTAAGGGGGGGTTGCCGGAAAAGGAGGTGAAGGGGTATGCCGGTCAGATACTCAAAGGGGTGAAACACATGCACGATTGTGGGTATGTGCATTGTGATTTAAAGCCGGACAACATATTGCTTGTGGGGGATGTAAATGTGGATGGAAAGCCTGTGTTTACGGCTAAAGTCGGTGACTTTGGCTTGGCTAAGAAGGAGGAGGAGGTTGAGGGAGGATGGAGAGGCACCCCTAACTATTTAGCTCCGGAGACTATAACCGAAAATAGACAAGAAAAACCTAGTGATATTTGGGCTGTTGGGTTAATTGTGCTTGAAATGCTAACCGGAGAGCGTGCCCGGCATCTTTTCTACGAGAGTGAGACTGGTTGTTTCTGCATTGTTGACAGAAGAATGAAGCCCGAAATTCCGGATACACTATCGACAGTTGCTATGGAGTTCATTGACTTTTGCTTGGAGGAAGTACCGGAGCATAGATGGACAGCTGAAGAGCTTCTGTCTCTTCCTTTTGTGGCAGCTGTTAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

312

Amino Acids

34.68

Weight (kDa)

5.59

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 43 - 307 4.5e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 45 - 302 1.9e-23 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 635
AccB7I CCANNNNNTGG 1 cut(s) 277
AccIII TCCGGA 3 cut(s) 113, 655, 817
AciI CCGC 3 cut(s) 23, 26, 224
AclWI GGATC 1 cut(s) 407
AcsI RAATTY 2 cut(s) 350, 813
AcuI CTGAAG 2 cut(s) 101, 915
AfaI GTAC 3 cut(s) 254, 269, 873
AfiI CCNNNNNNNGG 2 cut(s) 277, 417
AgeI ACCGGT 1 cut(s) 97
AgsI TTSAA 2 cut(s) 263, 725
AjnI CCWGG 1 cut(s) 270
AleI CACNNNNGTG 1 cut(s) 489
AluBI AGCT 6 cut(s) 105, 259, 653, 893, 901, 926
AluI AGCT 6 cut(s) 105, 259, 653, 893, 901, 926
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 3 cut(s) 653, 764, 912
AlwI GGATC 1 cut(s) 407
Aor13HI TCCGGA 3 cut(s) 113, 655, 817
AoxI GGCC 1 cut(s) 363
ApeKI GCWGC 1 cut(s) 923
ApoI RAATTY 2 cut(s) 350, 813
AsiGI ACCGGT 1 cut(s) 97
Asp700I GAANNNNTTC 1 cut(s) 900
AspS9I GGNCC 1 cut(s) 127
AsuC2I CCSGG 1 cut(s) 748
AsuHPI GGTGA 6 cut(s) 112, 296, 356, 451, 484, 596
AvaII GGWCC 1 cut(s) 127
BaeGI GKGCMC 1 cut(s) 748
BanI GGYRCC 1 cut(s) 635
BanII GRGCYC 1 cut(s) 79
Bbv12I GWGCWC 1 cut(s) 312
BccI CCATC 5 cut(s) 254, 275, 548, 621, 879
BceAI ACGGC 1 cut(s) 588
BciT130I CCWGG 1 cut(s) 272
BciVI GTATCC 1 cut(s) 814
BcnI CCSGG 1 cut(s) 748
BcoDI GTCTC 3 cut(s) 653, 764, 912
BfaI CTAG 1 cut(s) 690
BfuI GTATCC 1 cut(s) 814
BisI GCNGC 2 cut(s) 24, 924
BlsI GCNGC 2 cut(s) 25, 925
Bme1390I CCNGG 2 cut(s) 272, 748
Bme18I GGWCC 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 127
BmiI GGNNCC 6 cut(s) 7, 76, 112, 128, 163, 637
BmrFI CCNGG 2 cut(s) 272, 748
BmsI GCATC 1 cut(s) 760
BpuMI CCSGG 1 cut(s) 748
BsaJI CCNNGG 3 cut(s) 130, 207, 366
BsaWI WCCGGW 7 cut(s) 97, 113, 410, 655, 734, 817, 874
Bsc4I CCNNNNNNNGG 2 cut(s) 277, 417
Bse118I RCCGGY 2 cut(s) 97, 452
Bse1I ACTGG 2 cut(s) 250, 778
BseAI TCCGGA 3 cut(s) 113, 655, 817
BseBI CCWGG 1 cut(s) 272
BseDI CCNNGG 3 cut(s) 130, 207, 366
BseGI GGATG 5 cut(s) 246, 286, 547, 559, 632
BseLI CCNNNNNNNGG 2 cut(s) 277, 417
BseMII CTCAG 1 cut(s) 323
BseNI ACTGG 2 cut(s) 250, 778
BseRI GAGGAG 3 cut(s) 65, 623, 626
BseSI GKGCMC 1 cut(s) 748
BshFI GGCC 1 cut(s) 365
BshNI GGYRCC 1 cut(s) 635
BshTI ACCGGT 1 cut(s) 97
BsiHKAI GWGCWC 1 cut(s) 312
BslI CCNNNNNNNGG 2 cut(s) 277, 417
BsmAI GTCTC 3 cut(s) 653, 764, 912
BsmI GAATGC 1 cut(s) 176
BsnI GGCC 1 cut(s) 365
Bsp1286I GDGCHC 3 cut(s) 79, 312, 748
Bsp13I TCCGGA 3 cut(s) 113, 655, 817
Bsp143I GATC 1 cut(s) 399
Bsp19I CCATGG 1 cut(s) 207
BspACI CCGC 3 cut(s) 23, 26, 224
BspANI GGCC 1 cut(s) 365
BspCNI CTCAG 1 cut(s) 324
BspEI TCCGGA 3 cut(s) 113, 655, 817
BspLI GGNNCC 6 cut(s) 7, 76, 112, 128, 163, 637
BspPI GGATC 1 cut(s) 407
BspQI GCTCTTC 1 cut(s) 891
BspT107I GGYRCC 1 cut(s) 635
BsrFI RCCGGY 2 cut(s) 97, 452
BsrI ACTGG 2 cut(s) 250, 778
BssAI RCCGGY 2 cut(s) 97, 452
BssECI CCNNGG 3 cut(s) 130, 207, 366
BssMI GATC 1 cut(s) 399
BssT1I CCWWGG 2 cut(s) 207, 366
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 1 cut(s) 835
Bst6I CTCTTC 5 cut(s) 8, 77, 315, 891, 915
BstC8I GCNNGC 1 cut(s) 744
BstDEI CTNAG 2 cut(s) 332, 600
BstDSI CCRYGG 1 cut(s) 207
BstF5I GGATG 5 cut(s) 246, 286, 547, 559, 632
BstKTI GATC 1 cut(s) 402
BstMAI GTCTC 3 cut(s) 653, 764, 912
BstMBI GATC 1 cut(s) 399
BstMWI GCNNNNNNNGC 2 cut(s) 727, 748
BstNI CCWGG 1 cut(s) 272
BstNSI RCATGY 1 cut(s) 484
BstSCI CCNGG 2 cut(s) 270, 746
BstSLI GKGCMC 1 cut(s) 748
BsuI GTATCC 1 cut(s) 814
BsuRI GGCC 1 cut(s) 365
BtgI CCRYGG 1 cut(s) 207
BtsCI GGATG 5 cut(s) 246, 286, 547, 559, 632
Cac8I GCNNGC 1 cut(s) 744
Cfr10I RCCGGY 2 cut(s) 97, 452
Cfr13I GGNCC 1 cut(s) 127
Csp6I GTAC 3 cut(s) 253, 268, 872
CspAI ACCGGT 1 cut(s) 97
CviAII CATG 3 cut(s) 10, 208, 481
CviQI GTAC 3 cut(s) 253, 268, 872
DdeI CTNAG 2 cut(s) 332, 600
DpnI GATC 1 cut(s) 401
DpnII GATC 1 cut(s) 399
DraI TTTAAA 1 cut(s) 513
Eam1104I CTCTTC 5 cut(s) 8, 77, 315, 891, 915
EarI CTCTTC 5 cut(s) 8, 77, 315, 891, 915
EciI GGCGGA 2 cut(s) 41, 239
Eco130I CCWWGG 2 cut(s) 207, 366
Eco24I GRGCYC 1 cut(s) 79
Eco47I GGWCC 1 cut(s) 127
Eco57I CTGAAG 2 cut(s) 101, 915
EcoRII CCWGG 1 cut(s) 270
EcoT14I CCWWGG 2 cut(s) 207, 366
EcoT38I GRGCYC 1 cut(s) 79
ErhI CCWWGG 2 cut(s) 207, 366
FaeI CATG 3 cut(s) 13, 211, 484
FatI CATG 3 cut(s) 9, 207, 480
Fnu4HI GCNGC 2 cut(s) 24, 924
FokI GGATG 5 cut(s) 233, 293, 554, 566, 639
FriOI GRGCYC 1 cut(s) 79
Fsp4HI GCNGC 2 cut(s) 24, 924
FspBI CTAG 1 cut(s) 690
GluI GCNGC 2 cut(s) 24, 924
HaeIII GGCC 1 cut(s) 365
Hin1II CATG 3 cut(s) 13, 211, 484
HincII GTYRAC 1 cut(s) 793
HindII GTYRAC 1 cut(s) 793
HinfI GANTC 4 cut(s) 187, 236, 242, 287
HphI GGTGA 6 cut(s) 112, 296, 356, 451, 484, 596
Hpy166II GTNNAC 3 cut(s) 268, 570, 793
Hpy188I TCNGA 6 cut(s) 74, 131, 146, 241, 333, 459
Hpy188III TCNNGA 3 cut(s) 114, 656, 818
Hpy8I GTNNAC 3 cut(s) 268, 570, 793
HpyAV CCTTC 3 cut(s) 143, 436, 598
HpyCH4III ACNGT 1 cut(s) 835
HpyCH4V TGCA 4 cut(s) 176, 484, 502, 786
HpyF10VI GCNNNNNNNGC 2 cut(s) 727, 748
HpyF3I CTNAG 2 cut(s) 332, 600
Hsp92II CATG 3 cut(s) 13, 211, 484
Kpn2I TCCGGA 3 cut(s) 113, 655, 817
Kzo9I GATC 1 cut(s) 399
LguI GCTCTTC 1 cut(s) 891
LmnI GCTCC 4 cut(s) 5, 74, 658, 877
LweI GCATC 1 cut(s) 760
MaeI CTAG 1 cut(s) 690
MaeIII GTNAC 2 cut(s) 325, 584
MalI GATC 1 cut(s) 401
MboI GATC 1 cut(s) 399
MboII GAAGA 7 cut(s) 25, 75, 94, 332, 810, 902, 908
MhlI GDGCHC 3 cut(s) 79, 312, 748
MluCI AATT 4 cut(s) 275, 350, 714, 813
MlyI GAGTC 1 cut(s) 196
MmeI TCCRAC 1 cut(s) 52
MroI TCCGGA 3 cut(s) 113, 655, 817
MroXI GAANNNNTTC 1 cut(s) 900
MseI TTAA 2 cut(s) 512, 713
MslI CAYNNNNRTG 1 cut(s) 489
MspA1I CMGCKG 2 cut(s) 893, 926
MspR9I CCNGG 2 cut(s) 272, 748
Mva1269I GAATGC 1 cut(s) 176
MvaI CCWGG 1 cut(s) 272
MwoI GCNNNNNNNGC 2 cut(s) 727, 748
NciI CCSGG 1 cut(s) 748
NcoI CCATGG 1 cut(s) 207
NdeII GATC 1 cut(s) 399
NlaIII CATG 3 cut(s) 13, 211, 484
NlaIV GGNNCC 6 cut(s) 7, 76, 112, 128, 163, 637
NmeAIII GCCGAG 2 cut(s) 225, 418
NmuCI GTSAC 1 cut(s) 584
NspI RCATGY 1 cut(s) 484
OliI CACNNNNGTG 1 cut(s) 489
PciSI GCTCTTC 1 cut(s) 891
PctI GAATGC 1 cut(s) 176
PdmI GAANNNNTTC 1 cut(s) 900
PfeI GAWTC 3 cut(s) 236, 242, 287
PflMI CCANNNNNTGG 1 cut(s) 277
PinAI ACCGGT 1 cut(s) 97
PkrI GCNGC 2 cut(s) 25, 925
PleI GAGTC 1 cut(s) 195
PpsI GAGTC 1 cut(s) 195
Psp6I CCWGG 1 cut(s) 270
PspGI CCWGG 1 cut(s) 270
PspN4I GGNNCC 6 cut(s) 7, 76, 112, 128, 163, 637
PspPI GGNCC 1 cut(s) 127
PvuII CAGCTG 2 cut(s) 893, 926
RsaI GTAC 3 cut(s) 254, 269, 873
RsaNI GTAC 3 cut(s) 253, 268, 872
RseI CAYNNNNRTG 1 cut(s) 489
SapI GCTCTTC 1 cut(s) 891
SaqAI TTAA 2 cut(s) 512, 713
SatI GCNGC 2 cut(s) 24, 924
Sau3AI GATC 1 cut(s) 399
Sau96I GGNCC 1 cut(s) 127
SchI GAGTC 1 cut(s) 196
ScrFI CCNGG 2 cut(s) 272, 748
SduI GDGCHC 3 cut(s) 79, 312, 748
SfaNI GCATC 1 cut(s) 760
SinI GGWCC 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 489
Sse9I AATT 4 cut(s) 275, 350, 714, 813
SsiI CCGC 3 cut(s) 23, 26, 224
SspMI CTAG 1 cut(s) 690
StyD4I CCNGG 2 cut(s) 270, 746
StyI CCWWGG 2 cut(s) 207, 366
TaaI ACNGT 1 cut(s) 835
TaqI TCGA 2 cut(s) 306, 830
TasI AATT 4 cut(s) 275, 350, 714, 813
TauI GCSGC 1 cut(s) 26
TfiI GAWTC 3 cut(s) 236, 242, 287
Tru1I TTAA 2 cut(s) 512, 713
Tru9I TTAA 2 cut(s) 512, 713
TseFI GTSAC 1 cut(s) 584
TseI GCWGC 1 cut(s) 923
Tsp45I GTSAC 1 cut(s) 584
TspDTI ATGAA 6 cut(s) 26, 245, 249, 289, 818, 838
TspGWI ACGGA 1 cut(s) 154
Van91I CCANNNNNTGG 1 cut(s) 277
VpaK11BI GGWCC 1 cut(s) 127
XapI RAATTY 2 cut(s) 350, 813
XceI RCATGY 1 cut(s) 484
XcmI CCANNNNNNNNNTGG 1 cut(s) 278
XmnI GAANNNNTTC 1 cut(s) 900
XspI CTAG 1 cut(s) 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.