Rroxscaffold_2G00111450

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001294
Physical Location & Seq
Forward (+)
36046974 .. 36057924
10951 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00111450.1

Sequence Viewer

Length: 1005 bp
ATGCCTGTGATAACGCCATTCCCTATAAAGCATGAGGTTCGAAAAGAACGGGATGAAGAAGATGATGGATTCGAACAGTTGAGATGTGGATATGCGACCAAAAAGAGGTTATTACATAGAGAGAGAGAAGAGAGTAGCGATATGAAGAGGAAGGCGGAAGTGAGCTTGGGTTCAGGTCATAAGTGGAGGAGAGGAAAGAAGATCGGCGAAGGAGGCTTCGGTTCTGTGTTCGTTGCATTTTCCAAGAGGCCCTATATCATCTCCGATCATATGCCGGAGGCCATGGCTGTGAAATCAGCAAAGATCAAGGATTCCGATTCCATTCGATACGAGCTGGAGGTGTTTAGGGAGTTGAATAGCGTGGGCGGTTCTTGCTCCTTCATCATTGAGCACTATGGTGAGGAGAGATCTATAAGTTATAACTTGGCCTTGGAAGTAGCATGGGGAAGCAGCCTTGATAAGTTGTTAGAGAAAACCGGTCTTGGGTTGAGAGAATCCGATGTAAGGCGGTATACCGGTGATATTCTTAAAGGCATTAGACACATACACAAGAGTGGGTATGTCCAATTTGATTTGAAACCAGGTAACATCTTGCTTGTGGAAAATGAAGAAATTGATCGATATCGCTGTACCTATGTGGCCAAAGTTGGTGGCTTGGGGATGGCCAAGAAGGAGGAGGAGGTTCAGGAAGGCTGGAGAGGCACACCCATGTACTTCTCTCCTGAAACTGTCTCCGACAAGAAGCAAGAACAACCCAGTGACATATGGGCTGTGGGGTGTATTGTTCTGGAAATGCTTACGGGAGAGTGCCCCCGGAATCATTTCTATGATGGTGAGAGTGATACTCTCCACATTGTTGATGGAATGGAGCCAAAAATTCCTGATACGATCTCGAGTCTTGCTAAGGAGTTTCTAGAGCTTTGCCTAGCTGAGTTTCCTGAGCAAAGGTGGACAGCTGAAGAACTTCTAACTCATCCCTTCGTGGCAGATGTTGATAGAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

334

Amino Acids

38.11

Weight (kDa)

5.47

Isoelectric Point (pI)

52.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 63 - 328 6.6e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 65 - 323 1.5e-20 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 420
AccI GTMKAC 1 cut(s) 512
AciI CCGC 3 cut(s) 155, 366, 508
AcoI YGGCCR 2 cut(s) 639, 663
AcsI RAATTY 1 cut(s) 876
AcuI CTGAAG 1 cut(s) 978
AfaI GTAC 2 cut(s) 631, 713
AfiI CCNNNNNNNGG 3 cut(s) 105, 483, 504
AgeI ACCGGT 2 cut(s) 476, 515
AgsI TTSAA 2 cut(s) 355, 577
AjnI CCWGG 1 cut(s) 580
AleI CACNNNNGTG 2 cut(s) 396, 552
AluBI AGCT 5 cut(s) 165, 334, 919, 929, 956
AluI AGCT 5 cut(s) 165, 334, 919, 929, 956
Alw21I GWGCWC 1 cut(s) 393
Alw26I GTCTC 1 cut(s) 736
Ama87I CYCGRG 1 cut(s) 892
AoxI GGCC 5 cut(s) 248, 279, 426, 639, 663
ApeKI GCWGC 1 cut(s) 450
ApoI RAATTY 1 cut(s) 876
AsiGI ACCGGT 2 cut(s) 476, 515
Asp700I GAANNNNTTC 2 cut(s) 821, 963
AspS9I GGNCC 1 cut(s) 249
AsuC2I CCSGG 1 cut(s) 814
AsuHPI GGTGA 3 cut(s) 410, 530, 845
AsuII TTCGAA 2 cut(s) 40, 72
AvaI CYCGRG 1 cut(s) 892
BaeGI GKGCMC 1 cut(s) 812
BalI TGGCCA 2 cut(s) 641, 665
Bbv12I GWGCWC 1 cut(s) 393
BbvI GCAGC 1 cut(s) 462
BccI CCATC 4 cut(s) 59, 655, 824, 854
BcgI CGANNNNNNTGC 2 cut(s) 20, 54
BciT130I CCWGG 1 cut(s) 582
BcnI CCSGG 1 cut(s) 814
BcoDI GTCTC 1 cut(s) 736
BfaI CTAG 2 cut(s) 914, 926
BglII AGATCT 1 cut(s) 407
BisI GCNGC 1 cut(s) 451
BlsI GCNGC 1 cut(s) 452
Bme1390I CCNGG 2 cut(s) 582, 814
BmeT110I CYCGRG 1 cut(s) 892
BmgT120I GGNCC 1 cut(s) 249
BmiI GGNNCC 1 cut(s) 870
BmrFI CCNGG 2 cut(s) 582, 814
BmrI ACTGGG 1 cut(s) 750
BmuI ACTGGG 1 cut(s) 750
BplI GAGNNNNNCTC 2 cut(s) 829, 861
BpmI CTGGAG 2 cut(s) 356, 715
Bpu10I CCTNAGC 2 cut(s) 903, 939
Bpu14I TTCGAA 2 cut(s) 40, 72
BpuMI CCSGG 1 cut(s) 814
Bsa29I ATCGAT 1 cut(s) 619
BsaBI GATNNNNATC 1 cut(s) 621
BsaJI CCNNGG 3 cut(s) 282, 429, 812
BsaWI WCCGGW 2 cut(s) 476, 515
Bsc4I CCNNNNNNNGG 3 cut(s) 105, 483, 504
Bse118I RCCGGY 2 cut(s) 476, 515
Bse1I ACTGG 1 cut(s) 756
Bse8I GATNNNNATC 1 cut(s) 621
BseBI CCWGG 1 cut(s) 582
BseCI ATCGAT 1 cut(s) 619
BseDI CCNNGG 3 cut(s) 282, 429, 812
BseGI GGATG 3 cut(s) 58, 666, 973
BseJI GATNNNNATC 1 cut(s) 621
BseLI CCNNNNNNNGG 3 cut(s) 105, 483, 504
BseMII CTCAG 2 cut(s) 921, 930
BseNI ACTGG 1 cut(s) 756
BseRI GAGGAG 4 cut(s) 202, 416, 689, 692
BseSI GKGCMC 1 cut(s) 812
BseXI GCAGC 1 cut(s) 462
BshFI GGCC 5 cut(s) 250, 281, 428, 641, 665
BshTI ACCGGT 2 cut(s) 476, 515
BshVI ATCGAT 1 cut(s) 619
BsiHKAI GWGCWC 1 cut(s) 393
BsiHKCI CYCGRG 1 cut(s) 892
BsiSI CCGG 4 cut(s) 275, 477, 516, 814
BslI CCNNNNNNNGG 3 cut(s) 105, 483, 504
BsmAI GTCTC 1 cut(s) 736
BsnI GGCC 5 cut(s) 250, 281, 428, 641, 665
BsoBI CYCGRG 1 cut(s) 892
Bsp119I TTCGAA 2 cut(s) 40, 72
Bsp1286I GDGCHC 2 cut(s) 393, 812
Bsp143I GATC 6 cut(s) 201, 265, 303, 407, 616, 888
Bsp19I CCATGG 1 cut(s) 282
BspACI CCGC 3 cut(s) 155, 366, 508
BspANI GGCC 5 cut(s) 250, 281, 428, 641, 665
BspCNI CTCAG 2 cut(s) 922, 931
BspDI ATCGAT 1 cut(s) 619
BspLI GGNNCC 1 cut(s) 870
BspT104I TTCGAA 2 cut(s) 40, 72
BsrFI RCCGGY 2 cut(s) 476, 515
BsrI ACTGG 1 cut(s) 756
BssAI RCCGGY 2 cut(s) 476, 515
BssECI CCNNGG 3 cut(s) 282, 429, 812
BssMI GATC 6 cut(s) 201, 265, 303, 407, 616, 888
BssNAI GTATAC 1 cut(s) 513
BssT1I CCWWGG 2 cut(s) 282, 429
Bst1107I GTATAC 1 cut(s) 513
Bst2UI CCWGG 1 cut(s) 582
Bst4CI ACNGT 2 cut(s) 78, 730
Bst6I CTCTTC 2 cut(s) 123, 140
BstBI TTCGAA 2 cut(s) 40, 72
BstDEI CTNAG 3 cut(s) 903, 930, 939
BstDSI CCRYGG 1 cut(s) 282
BstF5I GGATG 3 cut(s) 58, 666, 973
BstKTI GATC 6 cut(s) 204, 268, 306, 410, 619, 891
BstMAI GTCTC 1 cut(s) 736
BstMBI GATC 6 cut(s) 201, 265, 303, 407, 616, 888
BstMWI GCNNNNNNNGC 3 cut(s) 213, 372, 699
BstNI CCWGG 1 cut(s) 582
BstSCI CCNGG 2 cut(s) 580, 812
BstSLI GKGCMC 1 cut(s) 812
BstV1I GCAGC 1 cut(s) 462
BstX2I RGATCY 1 cut(s) 407
BstYI RGATCY 1 cut(s) 407
BstZ17I GTATAC 1 cut(s) 513
Bsu15I ATCGAT 1 cut(s) 619
BsuRI GGCC 5 cut(s) 250, 281, 428, 641, 665
BsuTUI ATCGAT 1 cut(s) 619
BtgI CCRYGG 1 cut(s) 282
BtsCI GGATG 3 cut(s) 58, 666, 973
BtsIMutI CAGTG 1 cut(s) 763
Cfr10I RCCGGY 2 cut(s) 476, 515
Cfr13I GGNCC 1 cut(s) 249
ClaI ATCGAT 1 cut(s) 619
CsiI ACCWGGT 1 cut(s) 580
Csp6I GTAC 2 cut(s) 630, 712
CspAI ACCGGT 2 cut(s) 476, 515
CspCI CAANNNNNGTGG 2 cut(s) 631, 666
CviAII CATG 4 cut(s) 32, 283, 441, 709
CviQI GTAC 2 cut(s) 630, 712
DdeI CTNAG 3 cut(s) 903, 930, 939
DpnI GATC 6 cut(s) 203, 267, 305, 409, 618, 890
DpnII GATC 6 cut(s) 201, 265, 303, 407, 616, 888
EaeI YGGCCR 2 cut(s) 639, 663
Eam1104I CTCTTC 2 cut(s) 123, 140
EarI CTCTTC 2 cut(s) 123, 140
EciI GGCGGA 1 cut(s) 170
Eco130I CCWWGG 2 cut(s) 282, 429
Eco32I GATATC 1 cut(s) 623
Eco57I CTGAAG 1 cut(s) 978
Eco88I CYCGRG 1 cut(s) 892
EcoO109I RGGNCCY 1 cut(s) 249
EcoRII CCWGG 1 cut(s) 580
EcoRV GATATC 1 cut(s) 623
EcoT14I CCWWGG 2 cut(s) 282, 429
ErhI CCWWGG 2 cut(s) 282, 429
FaeI CATG 4 cut(s) 35, 286, 444, 712
FatI CATG 4 cut(s) 31, 282, 440, 708
FauNDI CATATG 2 cut(s) 270, 764
FblI GTMKAC 1 cut(s) 512
Fnu4HI GCNGC 1 cut(s) 451
FokI GGATG 3 cut(s) 65, 673, 960
Fsp4HI GCNGC 1 cut(s) 451
FspBI CTAG 2 cut(s) 914, 926
GluI GCNGC 1 cut(s) 451
GsuI CTGGAG 2 cut(s) 356, 715
HaeIII GGCC 5 cut(s) 250, 281, 428, 641, 665
HapII CCGG 4 cut(s) 275, 477, 516, 814
Hin1II CATG 4 cut(s) 35, 286, 444, 712
HinfI GANTC 6 cut(s) 69, 311, 317, 494, 817, 895
HpaII CCGG 4 cut(s) 275, 477, 516, 814
HphI GGTGA 3 cut(s) 410, 530, 845
Hpy166II GTNNAC 2 cut(s) 513, 951
Hpy188I TCNGA 4 cut(s) 265, 316, 499, 736
Hpy188III TCNNGA 7 cut(s) 686, 722, 788, 881, 892, 914, 938
Hpy8I GTNNAC 2 cut(s) 513, 951
HpyAV CCTTC 6 cut(s) 145, 203, 388, 664, 683, 988
HpyCH4III ACNGT 2 cut(s) 78, 730
HpyCH4V TGCA 1 cut(s) 236
HpyF10VI GCNNNNNNNGC 3 cut(s) 213, 372, 699
HpyF3I CTNAG 3 cut(s) 903, 930, 939
Hsp92II CATG 4 cut(s) 35, 286, 444, 712
Kzo9I GATC 6 cut(s) 201, 265, 303, 407, 616, 888
LmnI GCTCC 2 cut(s) 380, 868
Lsp1109I GCAGC 1 cut(s) 462
MabI ACCWGGT 1 cut(s) 580
MaeI CTAG 2 cut(s) 914, 926
MaeIII GTNAC 2 cut(s) 584, 758
MalI GATC 6 cut(s) 203, 267, 305, 409, 618, 890
MboI GATC 6 cut(s) 201, 265, 303, 407, 616, 888
MboII GAAGA 7 cut(s) 68, 71, 140, 157, 211, 620, 971
MflI RGATCY 1 cut(s) 407
MhlI GDGCHC 2 cut(s) 393, 812
MlsI TGGCCA 2 cut(s) 641, 665
MluCI AATT 3 cut(s) 566, 612, 876
MluNI TGGCCA 2 cut(s) 641, 665
MlyI GAGTC 1 cut(s) 904
MmeI TCCRAC 1 cut(s) 759
Mox20I TGGCCA 2 cut(s) 641, 665
MroXI GAANNNNTTC 2 cut(s) 821, 963
MscI TGGCCA 2 cut(s) 641, 665
MseI TTAA 1 cut(s) 528
MslI CAYNNNNRTG 5 cut(s) 287, 396, 552, 707, 825
Msp20I TGGCCA 2 cut(s) 641, 665
MspA1I CMGCKG 1 cut(s) 956
MspI CCGG 4 cut(s) 275, 477, 516, 814
MspR9I CCNGG 2 cut(s) 582, 814
MvaI CCWGG 1 cut(s) 582
MwoI GCNNNNNNNGC 3 cut(s) 213, 372, 699
NciI CCSGG 1 cut(s) 814
NcoI CCATGG 1 cut(s) 282
NdeI CATATG 2 cut(s) 270, 764
NdeII GATC 6 cut(s) 201, 265, 303, 407, 616, 888
NlaIII CATG 4 cut(s) 35, 286, 444, 712
NlaIV GGNNCC 1 cut(s) 870
NmuCI GTSAC 1 cut(s) 758
NspV TTCGAA 2 cut(s) 40, 72
OliI CACNNNNGTG 2 cut(s) 396, 552
PaeR7I CTCGAG 1 cut(s) 892
PdmI GAANNNNTTC 2 cut(s) 821, 963
PfeI GAWTC 5 cut(s) 69, 311, 317, 494, 817
PinAI ACCGGT 2 cut(s) 476, 515
PkrI GCNGC 1 cut(s) 452
PleI GAGTC 1 cut(s) 903
PpsI GAGTC 1 cut(s) 903
PsiI TTATAA 1 cut(s) 420
Psp6I CCWGG 1 cut(s) 580
PspGI CCWGG 1 cut(s) 580
PspN4I GGNNCC 1 cut(s) 870
PspPI GGNCC 1 cut(s) 249
PsuI RGATCY 1 cut(s) 407
PvuII CAGCTG 1 cut(s) 956
RsaI GTAC 2 cut(s) 631, 713
RsaNI GTAC 2 cut(s) 630, 712
RseI CAYNNNNRTG 5 cut(s) 287, 396, 552, 707, 825
SaqAI TTAA 1 cut(s) 528
SatI GCNGC 1 cut(s) 451
Sau3AI GATC 6 cut(s) 201, 265, 303, 407, 616, 888
Sau96I GGNCC 1 cut(s) 249
SchI GAGTC 1 cut(s) 904
ScrFI CCNGG 2 cut(s) 582, 814
SduI GDGCHC 2 cut(s) 393, 812
SexAI ACCWGGT 1 cut(s) 580
Sfr274I CTCGAG 1 cut(s) 892
SfuI TTCGAA 2 cut(s) 40, 72
SlaI CTCGAG 1 cut(s) 892
SmiMI CAYNNNNRTG 5 cut(s) 287, 396, 552, 707, 825
SmlI CTYRAG 1 cut(s) 892
SmoI CTYRAG 1 cut(s) 892
Sse9I AATT 3 cut(s) 566, 612, 876
SsiI CCGC 3 cut(s) 155, 366, 508
SspMI CTAG 2 cut(s) 914, 926
StyD4I CCNGG 2 cut(s) 580, 812
StyI CCWWGG 2 cut(s) 282, 429
TaaI ACNGT 2 cut(s) 78, 730
TaqI TCGA 5 cut(s) 40, 72, 325, 619, 893
TasI AATT 3 cut(s) 566, 612, 876
TatI WGTACW 1 cut(s) 711
TfiI GAWTC 5 cut(s) 69, 311, 317, 494, 817
Tru1I TTAA 1 cut(s) 528
Tru9I TTAA 1 cut(s) 528
TscAI CASTG 1 cut(s) 763
TseFI GTSAC 1 cut(s) 758
TseI GCWGC 1 cut(s) 450
Tsp45I GTSAC 1 cut(s) 758
TspDTI ATGAA 4 cut(s) 69, 158, 370, 621
TspRI CASTG 1 cut(s) 763
XapI RAATTY 1 cut(s) 876
XbaI TCTAGA 1 cut(s) 913
XcmI CCANNNNNNNNNTGG 1 cut(s) 857
XhoI CTCGAG 1 cut(s) 892
XmiI GTMKAC 1 cut(s) 512
XmnI GAANNNNTTC 2 cut(s) 821, 963
XspI CTAG 2 cut(s) 914, 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.