Prupe.3G098700_v2.0.a1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001294
Physical Location & Seq
Reverse (-)
7589235 .. 7590344
1110 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G098700.1

Sequence Viewer

Length: 1110 bp
ATGAAAAGAAAGGTTGAAGAAGAATTAGAAGGGGAAAAGCACAGCATGTGGAGTCATGGAGGGCACTGGCAGAGAGGGGAGTTTATTGGAGAAGGAAGCTTTGGTTCTGTTTTTCTCGCTACTCCCAAGAAACGAAGAAGGGGTGAGTTTAGCCGTATGGTCAATTTGCCTGCGATGATGGCGGTGAAATCGGCAAAGGTGTCTGCTTCGGAGTCTATTGAACATGAGGCAGGGGTTCTGTTTGAGATCAAGGGCTGCCCTTTCGTTATCGAGCGCTTCGGTGAAGAGACCACAACCACAGACAAGGGTGACAAGGTCTACAACTTGTTGTTAGAATTTGCTTCTGGAGGAACCCTTGATGGCCTCATACAAAAATCCAACGGTCTTGGATTGCCTGAAGATGATGTTAGAAGATATACAAGATCAATTCTTGAAGGGATTAGACACATTCACAAGTGTGACTATGTTCACTGCGATTTGAAGCCGGACAACATTCTGCTTGTGCCTACCACTACCACTACCACTACCACTACCACTACCACTACTACAGCTAGGTCTGGTGCTACAACTAGTTTTGTAGCCAAGATTGCAGATTTTGGGTTGGCTAAGAAAACCAAGGACAACTATAGTCGGTGGAGAGGCACTCCTAGGTATCTGTCCCCAGAGGCTTTATTTGATAACAAACAAGATCAGTCCTGTGATATTTGGGCTCTTGGTTGTATTGTGTTTGAGATGCTAACCGGAAACTCCCCCTGGGATTTGAAGCCTGGTTGTGATCTTGACAATTCCGTCGACGTGACGGTGTTTGATCATCTACGTACTTCCAAAATTCCGGCCGAGATCTCAGATGTGGCCAGGGATTTTCTCAAGAGTTGCCTTGCCATGAGGTCCTGTGAAAGATCAACAGCGGAAAGGCTCTTGTCTCATCCATTCGTAGCGCCGCCTCAACCATCAAAAGCGGGTCATGCCAAGTTGAAGGTGGTCAACTCATCTTTGGGCTATGCATATGGTGTCTCTTACTTCAAACCGAAAGCAGATTACCGTGCAAGCACTGCCACTGTTCCCAGAATTCATCCCTTACCGGGTTTTGAGATACCAGCTGCACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

370

Amino Acids

40.85

Weight (kDa)

8.32

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 788
AccI GTMKAC 2 cut(s) 318, 792
AciI CCGC 4 cut(s) 182, 908, 941, 959
AcoI YGGCCR 2 cut(s) 834, 852
AcsI RAATTY 3 cut(s) 335, 828, 1068
AcuI CTGAAG 1 cut(s) 417
AfaI GTAC 1 cut(s) 820
AfeI AGCGCT 1 cut(s) 275
AfiI CCNNNNNNNGG 1 cut(s) 1082
AgsI TTSAA 7 cut(s) 17, 221, 434, 481, 763, 976, 1024
AhlI ACTAGT 1 cut(s) 569
AjiI CACGTC 1 cut(s) 796
AjnI CCWGG 3 cut(s) 752, 766, 854
AjuI GAANNNNNNNTTGG 2 cut(s) 84, 116
AleI CACNNNNGTG 1 cut(s) 456
AluBI AGCT 3 cut(s) 99, 551, 1100
AluI AGCT 3 cut(s) 99, 551, 1100
Alw26I GTCTC 3 cut(s) 281, 927, 1018
Aor51HI AGCGCT 1 cut(s) 275
AoxI GGCC 3 cut(s) 361, 834, 852
ApeKI GCWGC 2 cut(s) 255, 1100
ApoI RAATTY 3 cut(s) 335, 828, 1068
AspA2I CCTAGG 1 cut(s) 647
AspLEI GCGC 2 cut(s) 276, 940
AspS9I GGNCC 1 cut(s) 888
AsuC2I CCSGG 1 cut(s) 1083
AsuHPI GGTGA 4 cut(s) 155, 196, 293, 320
AvaII GGWCC 1 cut(s) 888
AvrII CCTAGG 1 cut(s) 647
BaeGI GKGCMC 1 cut(s) 66
BalI TGGCCA 1 cut(s) 854
BanII GRGCYC 1 cut(s) 712
BbvI GCAGC 2 cut(s) 242, 1087
BccI CCATC 3 cut(s) 172, 353, 958
BceAI ACGGC 1 cut(s) 138
BciT130I CCWGG 3 cut(s) 754, 768, 856
BclI TGATCA 1 cut(s) 808
BcnI CCSGG 1 cut(s) 1083
BcoDI GTCTC 3 cut(s) 281, 927, 1018
BcuI ACTAGT 1 cut(s) 569
BfaI CTAG 3 cut(s) 552, 570, 648
BfmI CTRYAG 2 cut(s) 546, 625
BfoI RGCGCY 2 cut(s) 277, 941
BglII AGATCT 1 cut(s) 840
BisI GCNGC 3 cut(s) 256, 941, 1101
BlnI CCTAGG 1 cut(s) 647
BlsI GCNGC 3 cut(s) 257, 942, 1102
Bme1390I CCNGG 4 cut(s) 754, 768, 856, 1083
Bme18I GGWCC 1 cut(s) 888
BmgBI CACGTC 1 cut(s) 796
BmgT120I GGNCC 1 cut(s) 888
BmiI GGNNCC 1 cut(s) 352
BmrFI CCNGG 4 cut(s) 754, 768, 856, 1083
BmsI GCATC 1 cut(s) 723
BplI GAGNNNNNCTC 2 cut(s) 628, 660
BpmI CTGGAG 1 cut(s) 366
BpuEI CTTGAG 1 cut(s) 851
BpuMI CCSGG 1 cut(s) 1083
BsaAI YACGTR 1 cut(s) 818
BsaI GGTCTC 1 cut(s) 281
BsaJI CCNNGG 5 cut(s) 615, 647, 752, 753, 855
BsaWI WCCGGW 1 cut(s) 740
Bsc4I CCNNNNNNNGG 1 cut(s) 1082
Bse1I ACTGG 1 cut(s) 71
BseBI CCWGG 3 cut(s) 754, 768, 856
BseDI CCNNGG 5 cut(s) 615, 647, 752, 753, 855
BseGI GGATG 2 cut(s) 925, 1072
BseLI CCNNNNNNNGG 1 cut(s) 1082
BseMII CTCAG 1 cut(s) 858
BseNI ACTGG 1 cut(s) 71
BseSI GKGCMC 1 cut(s) 66
BseX3I CGGCCG 1 cut(s) 834
BseXI GCAGC 2 cut(s) 242, 1087
BsgI GTGCAG 1 cut(s) 1086
Bsh1285I CGRYCG 1 cut(s) 837
BshFI GGCC 3 cut(s) 363, 836, 854
BsiEI CGRYCG 1 cut(s) 837
BsiSI CCGG 4 cut(s) 485, 741, 833, 1082
BslFI GGGAC 1 cut(s) 643
BslI CCNNNNNNNGG 1 cut(s) 1082
BsmAI GTCTC 3 cut(s) 281, 927, 1018
BsmFI GGGAC 1 cut(s) 643
BsnI GGCC 3 cut(s) 363, 836, 854
Bso31I GGTCTC 1 cut(s) 281
Bsp1286I GDGCHC 2 cut(s) 66, 712
Bsp143I GATC 7 cut(s) 246, 422, 688, 775, 808, 840, 899
BspACI CCGC 4 cut(s) 182, 908, 941, 959
BspANI GGCC 3 cut(s) 363, 836, 854
BspCNI CTCAG 1 cut(s) 857
BspLI GGNNCC 1 cut(s) 352
BspTNI GGTCTC 1 cut(s) 281
BsrI ACTGG 1 cut(s) 71
BssECI CCNNGG 5 cut(s) 615, 647, 752, 753, 855
BssMI GATC 7 cut(s) 246, 422, 688, 775, 808, 840, 899
BssT1I CCWWGG 2 cut(s) 615, 647
Bst2UI CCWGG 3 cut(s) 754, 768, 856
Bst4CI ACNGT 4 cut(s) 383, 802, 1043, 1060
Bst6I CTCTTC 1 cut(s) 279
BstAPI GCANNNNNTGC 1 cut(s) 1052
BstBAI YACGTR 1 cut(s) 818
BstC8I GCNNGC 2 cut(s) 171, 1048
BstDEI CTNAG 2 cut(s) 606, 844
BstF5I GGATG 2 cut(s) 925, 1072
BstH2I RGCGCY 2 cut(s) 277, 941
BstHHI GCGC 2 cut(s) 276, 940
BstKTI GATC 7 cut(s) 249, 425, 691, 778, 811, 843, 902
BstMAI GTCTC 3 cut(s) 281, 927, 1018
BstMBI GATC 7 cut(s) 246, 422, 688, 775, 808, 840, 899
BstMCI CGRYCG 1 cut(s) 837
BstMWI GCNNNNNNNGC 4 cut(s) 179, 587, 965, 1052
BstNI CCWGG 3 cut(s) 754, 768, 856
BstNSI RCATGY 1 cut(s) 49
BstSCI CCNGG 4 cut(s) 752, 766, 854, 1081
BstSFI CTRYAG 2 cut(s) 546, 625
BstSLI GKGCMC 1 cut(s) 66
BstSNI TACGTA 1 cut(s) 818
BstV1I GCAGC 2 cut(s) 242, 1087
BstX2I RGATCY 1 cut(s) 840
BstYI RGATCY 1 cut(s) 840
BstZI CGGCCG 1 cut(s) 834
BsuRI GGCC 3 cut(s) 363, 836, 854
BtgZI GCGATG 1 cut(s) 188
BtrI CACGTC 1 cut(s) 796
BtsCI GGATG 2 cut(s) 925, 1072
BtsI GCAGTG 2 cut(s) 469, 1050
BtsIMutI CAGTG 4 cut(s) 64, 469, 1050, 1056
Cac8I GCNNGC 2 cut(s) 171, 1048
CfoI GCGC 2 cut(s) 276, 940
Cfr13I GGNCC 1 cut(s) 888
Csp6I GTAC 1 cut(s) 819
CviAII CATG 5 cut(s) 46, 56, 224, 883, 965
CviQI GTAC 1 cut(s) 819
DdeI CTNAG 2 cut(s) 606, 844
DpnI GATC 7 cut(s) 248, 424, 690, 777, 810, 842, 901
DpnII GATC 7 cut(s) 246, 422, 688, 775, 808, 840, 899
DrdI GACNNNNNNGTC 1 cut(s) 788
DseDI GACNNNNNNGTC 1 cut(s) 788
EaeI YGGCCR 2 cut(s) 834, 852
EagI CGGCCG 1 cut(s) 834
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
EclXI CGGCCG 1 cut(s) 834
Eco105I TACGTA 1 cut(s) 818
Eco130I CCWWGG 2 cut(s) 615, 647
Eco24I GRGCYC 1 cut(s) 712
Eco31I GGTCTC 1 cut(s) 281
Eco47I GGWCC 1 cut(s) 888
Eco47III AGCGCT 1 cut(s) 275
Eco52I CGGCCG 1 cut(s) 834
Eco57I CTGAAG 1 cut(s) 417
EcoO109I RGGNCCY 1 cut(s) 888
EcoRI GAATTC 1 cut(s) 1068
EcoRII CCWGG 3 cut(s) 752, 766, 854
EcoT14I CCWWGG 2 cut(s) 615, 647
EcoT22I ATGCAT 1 cut(s) 1006
EcoT38I GRGCYC 1 cut(s) 712
ErhI CCWWGG 2 cut(s) 615, 647
FaeI CATG 5 cut(s) 49, 59, 227, 886, 968
FaqI GGGAC 1 cut(s) 643
FatI CATG 5 cut(s) 45, 55, 223, 882, 964
FauI CCCGC 1 cut(s) 952
FauNDI CATATG 1 cut(s) 1006
FbaI TGATCA 1 cut(s) 808
FblI GTMKAC 2 cut(s) 318, 792
Fnu4HI GCNGC 3 cut(s) 256, 941, 1101
FokI GGATG 2 cut(s) 912, 1059
FriOI GRGCYC 1 cut(s) 712
Fsp4HI GCNGC 3 cut(s) 256, 941, 1101
FspBI CTAG 3 cut(s) 552, 570, 648
GlaI GCGC 2 cut(s) 275, 939
GluI GCNGC 3 cut(s) 256, 941, 1101
GsuI CTGGAG 1 cut(s) 366
HaeII RGCGCY 2 cut(s) 277, 941
HaeIII GGCC 3 cut(s) 363, 836, 854
HapII CCGG 4 cut(s) 485, 741, 833, 1082
HhaI GCGC 2 cut(s) 276, 940
Hin1II CATG 5 cut(s) 49, 59, 227, 886, 968
Hin6I GCGC 2 cut(s) 274, 938
HinP1I GCGC 2 cut(s) 274, 938
HincII GTYRAC 2 cut(s) 793, 985
HindII GTYRAC 2 cut(s) 793, 985
HindIII AAGCTT 1 cut(s) 97
HinfI GANTC 2 cut(s) 52, 212
HpaII CCGG 4 cut(s) 485, 741, 833, 1082
HphI GGTGA 4 cut(s) 155, 196, 293, 320
Hpy166II GTNNAC 4 cut(s) 319, 469, 793, 985
Hpy188I TCNGA 2 cut(s) 211, 847
Hpy188III TCNNGA 4 cut(s) 345, 431, 779, 868
Hpy8I GTNNAC 4 cut(s) 319, 469, 793, 985
Hpy99I CGWCG 2 cut(s) 794, 797
HpyAV CCTTC 5 cut(s) 23, 86, 132, 428, 970
HpyCH4III ACNGT 4 cut(s) 383, 802, 1043, 1060
HpyCH4IV ACGT 2 cut(s) 795, 817
HpyCH4V TGCA 4 cut(s) 590, 1004, 1046, 1103
HpyF10VI GCNNNNNNNGC 4 cut(s) 179, 587, 965, 1052
HpyF3I CTNAG 2 cut(s) 606, 844
HpySE526I ACGT 2 cut(s) 795, 817
Hsp92II CATG 5 cut(s) 49, 59, 227, 886, 968
HspAI GCGC 2 cut(s) 274, 938
Ksp22I TGATCA 1 cut(s) 808
Kzo9I GATC 7 cut(s) 246, 422, 688, 775, 808, 840, 899
Lsp1109I GCAGC 2 cut(s) 242, 1087
LweI GCATC 1 cut(s) 723
MaeI CTAG 3 cut(s) 552, 570, 648
MaeII ACGT 2 cut(s) 795, 817
MaeIII GTNAC 3 cut(s) 308, 458, 796
MalI GATC 7 cut(s) 248, 424, 690, 777, 810, 842, 901
MboI GATC 7 cut(s) 246, 422, 688, 775, 808, 840, 899
MboII GAAGA 6 cut(s) 29, 32, 147, 296, 410, 423
MflI RGATCY 1 cut(s) 840
MhlI GDGCHC 2 cut(s) 66, 712
MlsI TGGCCA 1 cut(s) 854
MluCI AATT 7 cut(s) 23, 163, 335, 426, 784, 828, 1068
MluNI TGGCCA 1 cut(s) 854
MlyI GAGTC 2 cut(s) 61, 221
MmeI TCCRAC 1 cut(s) 402
MnlI CCTC 9 cut(s) 53, 68, 220, 341, 374, 632, 658, 879, 954
Mox20I TGGCCA 1 cut(s) 854
Mph1103I ATGCAT 1 cut(s) 1006
MscI TGGCCA 1 cut(s) 854
MslI CAYNNNNRTG 1 cut(s) 456
Msp20I TGGCCA 1 cut(s) 854
MspA1I CMGCKG 2 cut(s) 908, 1100
MspI CCGG 4 cut(s) 485, 741, 833, 1082
MspR9I CCNGG 4 cut(s) 754, 768, 856, 1083
MvaI CCWGG 3 cut(s) 754, 768, 856
MwoI GCNNNNNNNGC 4 cut(s) 179, 587, 965, 1052
NciI CCSGG 1 cut(s) 1083
NdeI CATATG 1 cut(s) 1006
NdeII GATC 7 cut(s) 246, 422, 688, 775, 808, 840, 899
NlaIII CATG 5 cut(s) 49, 59, 227, 886, 968
NlaIV GGNNCC 1 cut(s) 352
NmeAIII GCCGAG 1 cut(s) 862
NmuCI GTSAC 3 cut(s) 308, 458, 796
NsiI ATGCAT 1 cut(s) 1006
NspI RCATGY 1 cut(s) 49
OliI CACNNNNGTG 1 cut(s) 456
PasI CCCWGGG 1 cut(s) 753
PflFI GACNNNGTC 1 cut(s) 314
PkrI GCNGC 3 cut(s) 257, 942, 1102
PleI GAGTC 2 cut(s) 60, 220
PpsI GAGTC 2 cut(s) 60, 220
Ppu21I YACGTR 1 cut(s) 818
PpuMI RGGWCCY 1 cut(s) 888
Psp5II RGGWCCY 1 cut(s) 888
Psp6I CCWGG 3 cut(s) 752, 766, 854
PspGI CCWGG 3 cut(s) 752, 766, 854
PspN4I GGNNCC 1 cut(s) 352
PspPI GGNCC 1 cut(s) 888
PspPPI RGGWCCY 1 cut(s) 888
PsuI RGATCY 1 cut(s) 840
PsyI GACNNNGTC 1 cut(s) 314
PvuII CAGCTG 1 cut(s) 1100
RsaI GTAC 1 cut(s) 820
RsaNI GTAC 1 cut(s) 819
RseI CAYNNNNRTG 1 cut(s) 456
SalI GTCGAC 1 cut(s) 791
SatI GCNGC 3 cut(s) 256, 941, 1101
Sau3AI GATC 7 cut(s) 246, 422, 688, 775, 808, 840, 899
Sau96I GGNCC 1 cut(s) 888
SchI GAGTC 2 cut(s) 61, 221
ScrFI CCNGG 4 cut(s) 754, 768, 856, 1083
SduI GDGCHC 2 cut(s) 66, 712
SfaNI GCATC 1 cut(s) 723
SfcI CTRYAG 2 cut(s) 546, 625
SgrDI CGTCGACG 1 cut(s) 791
SinI GGWCC 1 cut(s) 888
SmiMI CAYNNNNRTG 1 cut(s) 456
SmlI CTYRAG 1 cut(s) 866
SmoI CTYRAG 1 cut(s) 866
SnaBI TACGTA 1 cut(s) 818
SpeI ACTAGT 1 cut(s) 569
Sse9I AATT 7 cut(s) 23, 163, 335, 426, 784, 828, 1068
SsiI CCGC 4 cut(s) 182, 908, 941, 959
SspMI CTAG 3 cut(s) 552, 570, 648
StyD4I CCNGG 4 cut(s) 752, 766, 854, 1081
StyI CCWWGG 2 cut(s) 615, 647
TaaI ACNGT 4 cut(s) 383, 802, 1043, 1060
TaiI ACGT 2 cut(s) 798, 820
TaqI TCGA 2 cut(s) 270, 792
TasI AATT 7 cut(s) 23, 163, 335, 426, 784, 828, 1068
TauI GCSGC 1 cut(s) 943
TscAI CASTG 4 cut(s) 71, 476, 1057, 1063
TseFI GTSAC 3 cut(s) 308, 458, 796
TseI GCWGC 2 cut(s) 255, 1100
Tsp45I GTSAC 3 cut(s) 308, 458, 796
TspDTI ATGAA 2 cut(s) 17, 1061
TspGWI ACGGA 1 cut(s) 778
TspRI CASTG 4 cut(s) 71, 476, 1057, 1063
Tth111I GACNNNGTC 1 cut(s) 314
VpaK11BI GGWCC 1 cut(s) 888
XapI RAATTY 3 cut(s) 335, 828, 1068
XceI RCATGY 1 cut(s) 49
XcmI CCANNNNNNNNNTGG 1 cut(s) 976
XmaJI CCTAGG 1 cut(s) 647
XmiI GTMKAC 2 cut(s) 318, 792
XspI CTAG 3 cut(s) 552, 570, 648
Zsp2I ATGCAT 1 cut(s) 1006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.