Prupe.3G120700_v2.0.a1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001294
Physical Location & Seq
Forward (+)
10684975 .. 10685508
534 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G120700.1

Sequence Viewer

Length: 534 bp
ATGCCTACCACAACAACTAGTTCTGGTGGTACTAGTTTTGTAGCCAAGATTGCAGATTTTGGACTCGCTAAGAAAACCAATGTAAATTACAGTCGATGGAGAGGCACGCCTAGGTATCTGTCCCCAGATGCTTTTATTAATAACGAACAAGATCAGTCCTCTGATATTTGGTCTCTAGGTTGTATTGTGTTTGAGATACTAACCGGCAAGTCTCCCTGGGATTTGAAGCCTGGTTACAATCCAAACAATCTCCCTGACGTGTTGATGTTTGATCATCTACGTACTTGCAAAATTCCAACTGGAATCTCAGATGTGGCCTGGGATTTTCTCAAGAGTTGCCTTGCCATGAAGAGCAGGGAAAGATTAACAGCAGAAATTCTCTTGTCTCATCCATTCGTAGCGCAGCCACAACCATCAAAAGAGGGTCATACCAAGGTGAAGCTGGTTATTAACTCCTTTTTGGGCTATGCATCTGGTGTATGTTGCTTCAAACCGAACTCAGATTACCATGCAAACTCTGCCATGGTTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

178

Amino Acids

19.61

Weight (kDa)

8.59

Isoelectric Point (pI)

33.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 291, 375
AfaI GTAC 2 cut(s) 31, 283
AflIII ACRYGT 1 cut(s) 258
AgsI TTSAA 3 cut(s) 226, 490, 530
AhlI ACTAGT 2 cut(s) 17, 32
AjiI CACGTC 1 cut(s) 259
AjnI CCWGG 3 cut(s) 215, 229, 317
AluBI AGCT 1 cut(s) 442
AluI AGCT 1 cut(s) 442
Alw26I GTCTC 3 cut(s) 177, 216, 390
AoxI GGCC 1 cut(s) 315
ApeKI GCWGC 1 cut(s) 403
ApoI RAATTY 2 cut(s) 291, 375
AseI ATTAAT 1 cut(s) 138
AspA2I CCTAGG 1 cut(s) 110
AspLEI GCGC 1 cut(s) 403
AsuHPI GGTGA 1 cut(s) 448
AvrII CCTAGG 1 cut(s) 110
BarI GAAGNNNNNNTAC 2 cut(s) 218, 250
BbvI GCAGC 1 cut(s) 415
BccI CCATC 2 cut(s) 90, 421
BciT130I CCWGG 3 cut(s) 217, 231, 319
BclI TGATCA 1 cut(s) 271
BcoDI GTCTC 3 cut(s) 177, 216, 390
BcuI ACTAGT 2 cut(s) 17, 32
BfaI CTAG 4 cut(s) 18, 33, 111, 176
BisI GCNGC 1 cut(s) 404
BlnI CCTAGG 1 cut(s) 110
BlsI GCNGC 1 cut(s) 405
Bme1390I CCNGG 3 cut(s) 217, 231, 319
BmgBI CACGTC 1 cut(s) 259
BmrFI CCNGG 3 cut(s) 217, 231, 319
BmsI GCATC 2 cut(s) 118, 479
BpuEI CTTGAG 1 cut(s) 314
BsaAI YACGTR 1 cut(s) 281
BsaI GGTCTC 1 cut(s) 177
BsaJI CCNNGG 6 cut(s) 110, 215, 216, 318, 432, 522
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 1 cut(s) 304
BseBI CCWGG 3 cut(s) 217, 231, 319
BseDI CCNNGG 6 cut(s) 110, 215, 216, 318, 432, 522
BseGI GGATG 1 cut(s) 388
BseMII CTCAG 2 cut(s) 321, 513
BseNI ACTGG 1 cut(s) 304
BseXI GCAGC 1 cut(s) 415
BshFI GGCC 1 cut(s) 317
BsiSI CCGG 1 cut(s) 204
BslFI GGGAC 1 cut(s) 106
BsmAI GTCTC 3 cut(s) 177, 216, 390
BsmFI GGGAC 1 cut(s) 106
BsnI GGCC 1 cut(s) 317
Bso31I GGTCTC 1 cut(s) 177
Bsp143I GATC 2 cut(s) 151, 271
Bsp19I CCATGG 1 cut(s) 522
BspANI GGCC 1 cut(s) 317
BspCNI CTCAG 2 cut(s) 320, 512
BspQI GCTCTTC 1 cut(s) 344
BspTNI GGTCTC 1 cut(s) 177
BsrFI RCCGGY 1 cut(s) 203
BsrI ACTGG 1 cut(s) 304
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 6 cut(s) 110, 215, 216, 318, 432, 522
BssMI GATC 2 cut(s) 151, 271
BssT1I CCWWGG 3 cut(s) 110, 432, 522
Bst2UI CCWGG 3 cut(s) 217, 231, 319
Bst4CI ACNGT 1 cut(s) 92
Bst6I CTCTTC 1 cut(s) 344
BstAPI GCANNNNNTGC 1 cut(s) 518
BstBAI YACGTR 1 cut(s) 281
BstC8I GCNNGC 1 cut(s) 107
BstDEI CTNAG 3 cut(s) 69, 307, 499
BstDSI CCRYGG 1 cut(s) 522
BstF5I GGATG 1 cut(s) 388
BstHHI GCGC 1 cut(s) 403
BstKTI GATC 2 cut(s) 154, 274
BstMAI GTCTC 3 cut(s) 177, 216, 390
BstMBI GATC 2 cut(s) 151, 271
BstMWI GCNNNNNNNGC 2 cut(s) 50, 518
BstNI CCWGG 3 cut(s) 217, 231, 319
BstSCI CCNGG 3 cut(s) 215, 229, 317
BstSNI TACGTA 1 cut(s) 281
BstV1I GCAGC 1 cut(s) 415
BsuRI GGCC 1 cut(s) 317
BtgI CCRYGG 1 cut(s) 522
BtrI CACGTC 1 cut(s) 259
BtsCI GGATG 1 cut(s) 388
Cac8I GCNNGC 1 cut(s) 107
CfoI GCGC 1 cut(s) 403
Cfr10I RCCGGY 1 cut(s) 203
Csp6I GTAC 2 cut(s) 30, 282
CviAII CATG 3 cut(s) 346, 509, 523
CviJI RGCY 6 cut(s) 44, 229, 317, 406, 442, 465
CviKI_1 RGCY 6 cut(s) 44, 229, 317, 406, 442, 465
CviQI GTAC 2 cut(s) 30, 282
DdeI CTNAG 3 cut(s) 69, 307, 499
DpnI GATC 2 cut(s) 153, 273
DpnII GATC 2 cut(s) 151, 271
Eam1104I CTCTTC 1 cut(s) 344
EarI CTCTTC 1 cut(s) 344
Eco105I TACGTA 1 cut(s) 281
Eco130I CCWWGG 3 cut(s) 110, 432, 522
Eco31I GGTCTC 1 cut(s) 177
EcoRII CCWGG 3 cut(s) 215, 229, 317
EcoT14I CCWWGG 3 cut(s) 110, 432, 522
EcoT22I ATGCAT 1 cut(s) 472
ErhI CCWWGG 3 cut(s) 110, 432, 522
FaeI CATG 3 cut(s) 349, 512, 526
FaiI YATR 6 cut(s) 347, 429, 468, 481, 510, 524
FaqI GGGAC 1 cut(s) 106
FatI CATG 3 cut(s) 345, 508, 522
FbaI TGATCA 1 cut(s) 271
Fnu4HI GCNGC 1 cut(s) 404
FokI GGATG 1 cut(s) 375
Fsp4HI GCNGC 1 cut(s) 404
FspBI CTAG 4 cut(s) 18, 33, 111, 176
GlaI GCGC 1 cut(s) 402
GluI GCNGC 1 cut(s) 404
HaeIII GGCC 1 cut(s) 317
HapII CCGG 1 cut(s) 204
HhaI GCGC 1 cut(s) 403
Hin1II CATG 3 cut(s) 349, 512, 526
Hin6I GCGC 1 cut(s) 401
HinP1I GCGC 1 cut(s) 401
HinfI GANTC 2 cut(s) 63, 303
HpaII CCGG 1 cut(s) 204
HphI GGTGA 1 cut(s) 448
Hpy188I TCNGA 3 cut(s) 163, 310, 502
Hpy188III TCNNGA 1 cut(s) 331
HpyCH4III ACNGT 1 cut(s) 92
HpyCH4IV ACGT 2 cut(s) 258, 280
HpyCH4V TGCA 4 cut(s) 53, 288, 470, 512
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 518
HpyF3I CTNAG 3 cut(s) 69, 307, 499
HpySE526I ACGT 2 cut(s) 258, 280
Hsp92II CATG 3 cut(s) 349, 512, 526
HspAI GCGC 1 cut(s) 401
Ksp22I TGATCA 1 cut(s) 271
Kzo9I GATC 2 cut(s) 151, 271
LguI GCTCTTC 1 cut(s) 344
Lsp1109I GCAGC 1 cut(s) 415
LweI GCATC 2 cut(s) 118, 479
MaeI CTAG 4 cut(s) 18, 33, 111, 176
MaeII ACGT 2 cut(s) 258, 280
MaeIII GTNAC 1 cut(s) 233
MalI GATC 2 cut(s) 153, 273
MboI GATC 2 cut(s) 151, 271
MboII GAAGA 1 cut(s) 361
MluCI AATT 3 cut(s) 85, 291, 375
MlyI GAGTC 1 cut(s) 57
MmeI TCCRAC 1 cut(s) 320
MnlI CCTC 3 cut(s) 95, 169, 415
Mph1103I ATGCAT 1 cut(s) 472
MseI TTAA 3 cut(s) 138, 365, 450
MspI CCGG 1 cut(s) 204
MspR9I CCNGG 3 cut(s) 217, 231, 319
MvaI CCWGG 3 cut(s) 217, 231, 319
MwoI GCNNNNNNNGC 2 cut(s) 50, 518
NcoI CCATGG 1 cut(s) 522
NdeII GATC 2 cut(s) 151, 271
NlaIII CATG 3 cut(s) 349, 512, 526
NsiI ATGCAT 1 cut(s) 472
PasI CCCWGGG 1 cut(s) 216
PciSI GCTCTTC 1 cut(s) 344
PfeI GAWTC 1 cut(s) 303
PkrI GCNGC 1 cut(s) 405
PleI GAGTC 1 cut(s) 57
PpsI GAGTC 1 cut(s) 57
Ppu21I YACGTR 1 cut(s) 281
PshBI ATTAAT 1 cut(s) 138
Psp6I CCWGG 3 cut(s) 215, 229, 317
PspGI CCWGG 3 cut(s) 215, 229, 317
PsrI GAACNNNNNNTAC 2 cut(s) 488, 520
RsaI GTAC 2 cut(s) 31, 283
RsaNI GTAC 2 cut(s) 30, 282
SapI GCTCTTC 1 cut(s) 344
SaqAI TTAA 3 cut(s) 138, 365, 450
SatI GCNGC 1 cut(s) 404
Sau3AI GATC 2 cut(s) 151, 271
SchI GAGTC 1 cut(s) 57
ScrFI CCNGG 3 cut(s) 217, 231, 319
SetI ASST 6 cut(s) 116, 181, 261, 283, 438, 444
SfaNI GCATC 2 cut(s) 118, 479
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
SnaBI TACGTA 1 cut(s) 281
SpeI ACTAGT 2 cut(s) 17, 32
Sse9I AATT 3 cut(s) 85, 291, 375
SspMI CTAG 4 cut(s) 18, 33, 111, 176
StyD4I CCNGG 3 cut(s) 215, 229, 317
StyI CCWWGG 3 cut(s) 110, 432, 522
TaaI ACNGT 1 cut(s) 92
TaiI ACGT 2 cut(s) 261, 283
TaqI TCGA 1 cut(s) 94
TasI AATT 3 cut(s) 85, 291, 375
TfiI GAWTC 1 cut(s) 303
Tru1I TTAA 3 cut(s) 138, 365, 450
Tru9I TTAA 3 cut(s) 138, 365, 450
TseI GCWGC 1 cut(s) 403
TspDTI ATGAA 1 cut(s) 362
VspI ATTAAT 1 cut(s) 138
XapI RAATTY 2 cut(s) 291, 375
XcmI CCANNNNNNNNNTGG 1 cut(s) 439
XmaJI CCTAGG 1 cut(s) 110
XspI CTAG 4 cut(s) 18, 33, 111, 176
Zsp2I ATGCAT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.