Prupe.3G118700_v2.0.a1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001294
Physical Location & Seq
Reverse (-)
10267666 .. 10268700
1035 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G118700.1

Sequence Viewer

Length: 1035 bp
ATGAAAAGAAAGGTAGATGAAAAATTAGACGAAGAAAAGCACAGCATGTGCAGTCATGGAGTGCACTGGTCGAGAGGGAAGTTGATTGGAAAAGGAAGCTATGGTTTTGTTTTTCTTGCTACTCCCAGGAAACCAACAAAGGATGAGCATAGCCGCGGTATGCTCAATTTGCGAGAAATGATGGCGGTGAAATCAGCAAAGGTCTCTGCTTCTGAGTCCATTAAACATGAGTCAGACGTTCTCCTTGAGATCAAGGGTTGCCCTTTCATTATCGAGCGCTTAGGTGAAGAGACCACGGCCACAGACAAGGGTGACATGGTCTACAACTTGTTGTTAGAATTTGCTTCCGGAGGAACCCTAGATGGTCTCATACATAAATCAAAAGGTCATAAATTATCCGAATATGATGTTAGAAGATACACAAGGTCAATTCTTGAAGGGATTCAACACATTCACAAGTATGACTATGTTCACTGCGATTTGAAGCCGGACAACATTTTGCTTCTGCCTACCACAACAACTAGTTCTGGTGGTACTAGTTTTGTAGCCAAGATTGCAGATTTTGGACTCGCTAAGAAAACCAAAGTAAATTACAGTCGATGGAGAGGCACGCCTAGGTATCTGTCCCCAGAGGCTTTTATTAATAACAAACAAGATCAGTCCTCTGATATTTGGTCTCTAGGTTGTATTGTGTTTGAGATGCTAACCGGCAAGTCGCCCTGGGATTTGAAGCCTGGTTACAATCCAAACAATTTCCCTGACGTGTTGATGTTTGATCATCTACGTACTTGCAAACTTCCGACTGGGATCTCAGATATGGCCAGGGATTTTCTCAAGAGTTGCCTTGCCATGAATAGCAGGGAAAGATTAACAGCGGAAAGTCTCTTGTCTCATCCATTCGTAGCGCAGCCACAATCATCAAAAGAGGGTCATACCAAGGTGAAGCTGATTAACTCCTTTTTGGGCTACGCATCTGGTGTATGTTGCTTCAAACCGAGCTCAGATTACCATGCACACTCTGCCATTGTACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

345

Amino Acids

38.58

Weight (kDa)

8.93

Isoelectric Point (pI)

37.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 321
AccII CGCG 1 cut(s) 156
AccIII TCCGGA 1 cut(s) 347
AciI CCGC 4 cut(s) 154, 156, 185, 875
AclWI GGATC 1 cut(s) 815
AcoI YGGCCR 2 cut(s) 297, 819
AcsI RAATTY 1 cut(s) 338
AfaI GTAC 3 cut(s) 535, 787, 1029
AfeI AGCGCT 1 cut(s) 278
AflIII ACRYGT 1 cut(s) 762
AgsI TTSAA 5 cut(s) 437, 446, 484, 730, 991
AhlI ACTAGT 2 cut(s) 521, 536
AjiI CACGTC 1 cut(s) 763
AjnI CCWGG 4 cut(s) 125, 719, 733, 821
AluBI AGCT 3 cut(s) 99, 946, 999
AluI AGCT 3 cut(s) 99, 946, 999
Alw21I GWGCWC 2 cut(s) 66, 1001
Alw26I GTCTC 6 cut(s) 208, 284, 371, 681, 887, 894
Alw44I GTGCAC 1 cut(s) 62
AlwI GGATC 1 cut(s) 815
Aor13HI TCCGGA 1 cut(s) 347
Aor51HI AGCGCT 1 cut(s) 278
AoxI GGCC 2 cut(s) 297, 819
ApaLI GTGCAC 1 cut(s) 62
ApeKI GCWGC 1 cut(s) 907
ApoI RAATTY 1 cut(s) 338
AseI ATTAAT 1 cut(s) 642
Asp700I GAANNNNTTC 1 cut(s) 441
AspA2I CCTAGG 1 cut(s) 614
AspLEI GCGC 2 cut(s) 279, 907
AsuHPI GGTGA 4 cut(s) 199, 296, 323, 952
AvrII CCTAGG 1 cut(s) 614
BaeGI GKGCMC 1 cut(s) 66
BalI TGGCCA 1 cut(s) 821
BanII GRGCYC 1 cut(s) 1001
BarI GAAGNNNNNNTAC 2 cut(s) 722, 754
Bbv12I GWGCWC 2 cut(s) 66, 1001
BbvI GCAGC 1 cut(s) 919
BccI CCATC 3 cut(s) 175, 356, 594
BceAI ACGGC 1 cut(s) 312
BciT130I CCWGG 4 cut(s) 127, 721, 735, 823
BclI TGATCA 1 cut(s) 775
BcoDI GTCTC 6 cut(s) 208, 284, 371, 681, 887, 894
BcuI ACTAGT 2 cut(s) 521, 536
BfaI CTAG 5 cut(s) 359, 522, 537, 615, 680
BfoI RGCGCY 1 cut(s) 280
BisI GCNGC 2 cut(s) 154, 908
BlnI CCTAGG 1 cut(s) 614
BlsI GCNGC 2 cut(s) 155, 909
Bme1390I CCNGG 4 cut(s) 127, 721, 735, 823
BmgBI CACGTC 1 cut(s) 763
BmiI GGNNCC 1 cut(s) 355
BmrFI CCNGG 4 cut(s) 127, 721, 735, 823
BmrI ACTGGG 1 cut(s) 813
BmsI GCATC 2 cut(s) 690, 980
BmuI ACTGGG 1 cut(s) 813
Bpu10I CCTNAGC 1 cut(s) 280
BpuEI CTTGAG 2 cut(s) 266, 818
BsaAI YACGTR 1 cut(s) 785
BsaI GGTCTC 4 cut(s) 208, 284, 371, 681
BsaJI CCNNGG 8 cut(s) 125, 154, 294, 614, 719, 720, 822, 936
BsaWI WCCGGW 1 cut(s) 347
Bse118I RCCGGY 1 cut(s) 707
Bse1I ACTGG 2 cut(s) 71, 808
BseAI TCCGGA 1 cut(s) 347
BseBI CCWGG 4 cut(s) 127, 721, 735, 823
BseDI CCNNGG 8 cut(s) 125, 154, 294, 614, 719, 720, 822, 936
BseGI GGATG 2 cut(s) 148, 892
BseMII CTCAG 3 cut(s) 204, 825, 1014
BseNI ACTGG 2 cut(s) 71, 808
BseSI GKGCMC 1 cut(s) 66
BseXI GCAGC 1 cut(s) 919
BsgI GTGCAG 1 cut(s) 70
Bsh1236I CGCG 1 cut(s) 156
BshFI GGCC 2 cut(s) 299, 821
BsiHKAI GWGCWC 2 cut(s) 66, 1001
BsiSI CCGG 3 cut(s) 348, 488, 708
BslFI GGGAC 1 cut(s) 610
BsmAI GTCTC 6 cut(s) 208, 284, 371, 681, 887, 894
BsmFI GGGAC 1 cut(s) 610
BsnI GGCC 2 cut(s) 299, 821
Bso31I GGTCTC 4 cut(s) 208, 284, 371, 681
Bsp1286I GDGCHC 2 cut(s) 66, 1001
Bsp13I TCCGGA 1 cut(s) 347
Bsp1407I TGTACA 1 cut(s) 1027
Bsp143I GATC 4 cut(s) 249, 655, 775, 807
BspACI CCGC 4 cut(s) 154, 156, 185, 875
BspANI GGCC 2 cut(s) 299, 821
BspCNI CTCAG 3 cut(s) 205, 824, 1013
BspEI TCCGGA 1 cut(s) 347
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 1 cut(s) 355
BspPI GGATC 1 cut(s) 815
BspTNI GGTCTC 4 cut(s) 208, 284, 371, 681
BsrFI RCCGGY 1 cut(s) 707
BsrGI TGTACA 1 cut(s) 1027
BsrI ACTGG 2 cut(s) 71, 808
BssAI RCCGGY 1 cut(s) 707
BssECI CCNNGG 8 cut(s) 125, 154, 294, 614, 719, 720, 822, 936
BssMI GATC 4 cut(s) 249, 655, 775, 807
BssT1I CCWWGG 2 cut(s) 614, 936
Bst2UI CCWGG 4 cut(s) 127, 721, 735, 823
Bst4CI ACNGT 1 cut(s) 596
Bst6I CTCTTC 1 cut(s) 282
BstAPI GCANNNNNTGC 1 cut(s) 1019
BstAUI TGTACA 1 cut(s) 1027
BstBAI YACGTR 1 cut(s) 785
BstC8I GCNNGC 1 cut(s) 611
BstDEI CTNAG 5 cut(s) 213, 280, 573, 811, 1000
BstDSI CCRYGG 2 cut(s) 154, 294
BstF5I GGATG 2 cut(s) 148, 892
BstFNI CGCG 1 cut(s) 156
BstH2I RGCGCY 1 cut(s) 280
BstHHI GCGC 2 cut(s) 279, 907
BstKTI GATC 4 cut(s) 252, 658, 778, 810
BstMAI GTCTC 6 cut(s) 208, 284, 371, 681, 887, 894
BstMBI GATC 4 cut(s) 249, 655, 775, 807
BstMWI GCNNNNNNNGC 3 cut(s) 169, 554, 1019
BstNI CCWGG 4 cut(s) 127, 721, 735, 823
BstNSI RCATGY 1 cut(s) 49
BstSCI CCNGG 4 cut(s) 125, 719, 733, 821
BstSLI GKGCMC 1 cut(s) 66
BstSNI TACGTA 1 cut(s) 785
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 1 cut(s) 919
BstX2I RGATCY 1 cut(s) 807
BstYI RGATCY 1 cut(s) 807
BsuRI GGCC 2 cut(s) 299, 821
BtgI CCRYGG 2 cut(s) 154, 294
BtrI CACGTC 1 cut(s) 763
BtsCI GGATG 2 cut(s) 148, 892
BtsI GCAGTG 1 cut(s) 472
BtsIMutI CAGTG 2 cut(s) 64, 472
Cac8I GCNNGC 1 cut(s) 611
CfoI GCGC 2 cut(s) 279, 907
Cfr10I RCCGGY 1 cut(s) 707
Cfr42I CCGCGG 1 cut(s) 157
Csp6I GTAC 3 cut(s) 534, 786, 1028
CviAII CATG 6 cut(s) 46, 56, 227, 316, 850, 1010
CviQI GTAC 3 cut(s) 534, 786, 1028
DdeI CTNAG 5 cut(s) 213, 280, 573, 811, 1000
DpnI GATC 4 cut(s) 251, 657, 777, 809
DpnII GATC 4 cut(s) 249, 655, 775, 807
EaeI YGGCCR 2 cut(s) 297, 819
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
Ecl136II GAGCTC 1 cut(s) 999
Eco105I TACGTA 1 cut(s) 785
Eco130I CCWWGG 2 cut(s) 614, 936
Eco24I GRGCYC 1 cut(s) 1001
Eco31I GGTCTC 4 cut(s) 208, 284, 371, 681
Eco47III AGCGCT 1 cut(s) 278
Eco53kI GAGCTC 1 cut(s) 999
EcoICRI GAGCTC 1 cut(s) 999
EcoRII CCWGG 4 cut(s) 125, 719, 733, 821
EcoT14I CCWWGG 2 cut(s) 614, 936
EcoT38I GRGCYC 1 cut(s) 1001
ErhI CCWWGG 2 cut(s) 614, 936
FaeI CATG 6 cut(s) 49, 59, 230, 319, 853, 1013
FaqI GGGAC 1 cut(s) 610
FatI CATG 6 cut(s) 45, 55, 226, 315, 849, 1009
FbaI TGATCA 1 cut(s) 775
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 2 cut(s) 154, 908
FokI GGATG 2 cut(s) 155, 879
FriOI GRGCYC 1 cut(s) 1001
Fsp4HI GCNGC 2 cut(s) 154, 908
FspBI CTAG 5 cut(s) 359, 522, 537, 615, 680
GlaI GCGC 2 cut(s) 278, 906
GluI GCNGC 2 cut(s) 154, 908
HaeII RGCGCY 1 cut(s) 280
HaeIII GGCC 2 cut(s) 299, 821
HapII CCGG 3 cut(s) 348, 488, 708
HhaI GCGC 2 cut(s) 279, 907
Hin1II CATG 6 cut(s) 49, 59, 230, 319, 853, 1013
Hin6I GCGC 2 cut(s) 277, 905
HinP1I GCGC 2 cut(s) 277, 905
HinfI GANTC 4 cut(s) 215, 230, 442, 567
HpaII CCGG 3 cut(s) 348, 488, 708
HphI GGTGA 4 cut(s) 199, 296, 323, 952
Hpy166II GTNNAC 3 cut(s) 64, 322, 472
Hpy188I TCNGA 7 cut(s) 214, 235, 400, 667, 801, 814, 1003
Hpy188III TCNNGA 4 cut(s) 72, 348, 434, 835
Hpy8I GTNNAC 3 cut(s) 64, 322, 472
HpyAV CCTTC 1 cut(s) 431
HpyCH4III ACNGT 1 cut(s) 596
HpyCH4IV ACGT 3 cut(s) 237, 762, 784
HpyCH4V TGCA 5 cut(s) 51, 64, 557, 792, 1013
HpyF10VI GCNNNNNNNGC 3 cut(s) 169, 554, 1019
HpyF3I CTNAG 5 cut(s) 213, 280, 573, 811, 1000
HpySE526I ACGT 3 cut(s) 237, 762, 784
Hsp92II CATG 6 cut(s) 49, 59, 230, 319, 853, 1013
HspAI GCGC 2 cut(s) 277, 905
Kpn2I TCCGGA 1 cut(s) 347
Ksp22I TGATCA 1 cut(s) 775
KspI CCGCGG 1 cut(s) 157
Kzo9I GATC 4 cut(s) 249, 655, 775, 807
Lsp1109I GCAGC 1 cut(s) 919
LweI GCATC 2 cut(s) 690, 980
MaeI CTAG 5 cut(s) 359, 522, 537, 615, 680
MaeII ACGT 3 cut(s) 237, 762, 784
MaeIII GTNAC 2 cut(s) 311, 737
MalI GATC 4 cut(s) 251, 657, 777, 809
MboI GATC 4 cut(s) 249, 655, 775, 807
MboII GAAGA 3 cut(s) 44, 299, 426
MflI RGATCY 1 cut(s) 807
MhlI GDGCHC 2 cut(s) 66, 1001
MlsI TGGCCA 1 cut(s) 821
MluCI AATT 7 cut(s) 23, 166, 338, 392, 429, 589, 751
MluNI TGGCCA 1 cut(s) 821
MlyI GAGTC 3 cut(s) 224, 239, 561
MmeI TCCRAC 1 cut(s) 824
MnlI CCTC 6 cut(s) 68, 344, 599, 625, 673, 919
Mox20I TGGCCA 1 cut(s) 821
MroI TCCGGA 1 cut(s) 347
MroXI GAANNNNTTC 1 cut(s) 441
MscI TGGCCA 1 cut(s) 821
MseI TTAA 4 cut(s) 222, 642, 869, 951
MslI CAYNNNNRTG 1 cut(s) 459
Msp20I TGGCCA 1 cut(s) 821
MspA1I CMGCKG 2 cut(s) 156, 875
MspI CCGG 3 cut(s) 348, 488, 708
MspR9I CCNGG 4 cut(s) 127, 721, 735, 823
MvaI CCWGG 4 cut(s) 127, 721, 735, 823
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 3 cut(s) 169, 554, 1019
NdeII GATC 4 cut(s) 249, 655, 775, 807
NlaIII CATG 6 cut(s) 49, 59, 230, 319, 853, 1013
NlaIV GGNNCC 1 cut(s) 355
NmuCI GTSAC 1 cut(s) 311
NspI RCATGY 1 cut(s) 49
PasI CCCWGGG 1 cut(s) 720
PdmI GAANNNNTTC 1 cut(s) 441
PfeI GAWTC 1 cut(s) 442
PflFI GACNNNGTC 1 cut(s) 317
PkrI GCNGC 2 cut(s) 155, 909
PleI GAGTC 3 cut(s) 223, 238, 561
PpsI GAGTC 3 cut(s) 223, 238, 561
Ppu21I YACGTR 1 cut(s) 785
PshBI ATTAAT 1 cut(s) 642
Psp124BI GAGCTC 1 cut(s) 1001
Psp6I CCWGG 4 cut(s) 125, 719, 733, 821
PspGI CCWGG 4 cut(s) 125, 719, 733, 821
PspN4I GGNNCC 1 cut(s) 355
PsuI RGATCY 1 cut(s) 807
PsyI GACNNNGTC 1 cut(s) 317
RsaI GTAC 3 cut(s) 535, 787, 1029
RsaNI GTAC 3 cut(s) 534, 786, 1028
RseI CAYNNNNRTG 1 cut(s) 459
SacI GAGCTC 1 cut(s) 1001
SacII CCGCGG 1 cut(s) 157
SaqAI TTAA 4 cut(s) 222, 642, 869, 951
SatI GCNGC 2 cut(s) 154, 908
Sau3AI GATC 4 cut(s) 249, 655, 775, 807
SchI GAGTC 3 cut(s) 224, 239, 561
ScrFI CCNGG 4 cut(s) 127, 721, 735, 823
SduI GDGCHC 2 cut(s) 66, 1001
SfaNI GCATC 2 cut(s) 690, 980
Sfr303I CCGCGG 1 cut(s) 157
SgrBI CCGCGG 1 cut(s) 157
SmiMI CAYNNNNRTG 1 cut(s) 459
SmlI CTYRAG 2 cut(s) 245, 833
SmoI CTYRAG 2 cut(s) 245, 833
SnaBI TACGTA 1 cut(s) 785
SpeI ACTAGT 2 cut(s) 521, 536
Sse9I AATT 7 cut(s) 23, 166, 338, 392, 429, 589, 751
SsiI CCGC 4 cut(s) 154, 156, 185, 875
SspMI CTAG 5 cut(s) 359, 522, 537, 615, 680
SstI GAGCTC 1 cut(s) 1001
StyD4I CCNGG 4 cut(s) 125, 719, 733, 821
StyI CCWWGG 2 cut(s) 614, 936
TaaI ACNGT 1 cut(s) 596
TaiI ACGT 3 cut(s) 240, 765, 787
TaqI TCGA 3 cut(s) 71, 273, 598
TasI AATT 7 cut(s) 23, 166, 338, 392, 429, 589, 751
TatI WGTACW 1 cut(s) 1027
TauI GCSGC 1 cut(s) 156
TfiI GAWTC 1 cut(s) 442
Tru1I TTAA 4 cut(s) 222, 642, 869, 951
Tru9I TTAA 4 cut(s) 222, 642, 869, 951
TscAI CASTG 2 cut(s) 71, 479
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 1 cut(s) 907
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 4 cut(s) 17, 33, 256, 866
TspRI CASTG 2 cut(s) 71, 479
Tth111I GACNNNGTC 1 cut(s) 317
VneI GTGCAC 1 cut(s) 62
VspI ATTAAT 1 cut(s) 642
XapI RAATTY 1 cut(s) 338
XceI RCATGY 1 cut(s) 49
XmaJI CCTAGG 1 cut(s) 614
XmiI GTMKAC 1 cut(s) 321
XmnI GAANNNNTTC 1 cut(s) 441
XspI CTAG 5 cut(s) 359, 522, 537, 615, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.