RLG00000028001

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001294
Physical Location & Seq
Forward (+)
17604172 .. 17605053
882 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028001

Sequence Viewer

Length: 720 bp
ATGAAAAGGAAGGTGGAAGTGAGTTTTGGTTCAGGTTACAAGTGGATAAGAGGAAACAAGATCGGAGAAGGAGGCTTCGGCTCGGTCAAGGATTCAGATTCCATTCGATACGAGCTCGAGGTGTTTAGGGAGTTGACTAACAAGGGTGGTTCTTGCCCCTTCATCATTGAGCACTATGGTGAAGAGGTGACATGGAGTGACGACGGGGAAGAGGTCTATAACTTGGCCTTGGAAGTTGCATGGGGAAGCCTTGCGAGGTTGATAAAGATTATCGGTCATGGGTTGCCAGAATCCGATGTGAGGCAGTACACCGGTGACATTCTTGAAGGGGTGACCGTTCGACACATACACAAAGTGGCGAAAGTGGGTGACTTGGGGTTGGCCAAGAAGGAGGAGGTTCAAGAAAGGTGGAGAGGCACTCCCATGTACTTGTCTCCGGAGACTGTCTTGTACAATGAGCAAGAACAAGCCAGTGATATTTGTGCTGTGGGGTGTATTGTTCTTGAAATGCCATCTGGAGAGCGCCCCGGGAATCATTTCTACAGTCATGAGAGTGATACTCTCTACGTTGTTGATGGAATGGAGCCTAAAATTCCTGATACGATCTCGAGTCTTGCTAGGGAGTTTCTAGGGTTTTGCCTAGCCAAGGAAACTCCGGAAAGGTGGACAGCTGAAGAGCTTCTATCTCATCCCTTTGTGGCAAATGTTGTTAGATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

240

Amino Acids

26.86

Weight (kDa)

5.04

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 117 - 229 1.7e-06 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 119 - 233 4e-16 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 436, 655
AcoI YGGCCR 1 cut(s) 381
AcsI RAATTY 1 cut(s) 591
AcuI CTGAAG 1 cut(s) 693
AdeI CACNNNGTG 1 cut(s) 355
AfaI GTAC 3 cut(s) 308, 428, 452
AfiI CCNNNNNNNGG 2 cut(s) 300, 646
AgeI ACCGGT 1 cut(s) 311
AgsI TTSAA 3 cut(s) 326, 401, 506
AjuI GAANNNNNNNTTGG 2 cut(s) 9, 41
AleI CACNNNNGTG 1 cut(s) 177
AluBI AGCT 3 cut(s) 115, 671, 679
AluI AGCT 3 cut(s) 115, 671, 679
Alw21I GWGCWC 2 cut(s) 117, 174
Alw26I GTCTC 2 cut(s) 434, 438
Ama87I CYCGRG 3 cut(s) 116, 527, 607
Aor13HI TCCGGA 2 cut(s) 436, 655
AoxI GGCC 2 cut(s) 225, 381
ApoI RAATTY 1 cut(s) 591
AsiGI ACCGGT 1 cut(s) 311
Asp700I GAANNNNTTC 2 cut(s) 536, 678
AspLEI GCGC 1 cut(s) 525
AsuC2I CCSGG 2 cut(s) 528, 529
AsuHPI GGTGA 5 cut(s) 191, 199, 326, 343, 380
AvaI CYCGRG 3 cut(s) 116, 527, 607
BalI TGGCCA 1 cut(s) 383
BanII GRGCYC 1 cut(s) 117
Bbv12I GWGCWC 2 cut(s) 117, 174
BccI CCATC 2 cut(s) 520, 569
BcnI CCSGG 2 cut(s) 528, 529
BcoDI GTCTC 2 cut(s) 434, 438
BfaI CTAG 3 cut(s) 618, 629, 641
BfmI CTRYAG 1 cut(s) 541
BfoI RGCGCY 1 cut(s) 526
Bme1390I CCNGG 2 cut(s) 528, 529
BmeT110I CYCGRG 3 cut(s) 116, 527, 607
BmiI GGNNCC 1 cut(s) 585
BmrFI CCNGG 2 cut(s) 528, 529
BplI GAGNNNNNCTC 4 cut(s) 403, 435, 544, 576
BpmI CTGGAG 1 cut(s) 537
BpuMI CCSGG 2 cut(s) 528, 529
BsaJI CCNNGG 4 cut(s) 228, 526, 527, 645
BsaWI WCCGGW 3 cut(s) 311, 436, 655
Bsc4I CCNNNNNNNGG 2 cut(s) 300, 646
Bse118I RCCGGY 1 cut(s) 311
Bse1I ACTGG 1 cut(s) 471
BseAI TCCGGA 2 cut(s) 436, 655
BseDI CCNNGG 4 cut(s) 228, 526, 527, 645
BseGI GGATG 1 cut(s) 688
BseLI CCNNNNNNNGG 2 cut(s) 300, 646
BseNI ACTGG 1 cut(s) 471
BseRI GAGGAG 1 cut(s) 407
BshFI GGCC 2 cut(s) 227, 383
BshTI ACCGGT 1 cut(s) 311
BsiHKAI GWGCWC 2 cut(s) 117, 174
BsiHKCI CYCGRG 3 cut(s) 116, 527, 607
BsiSI CCGG 4 cut(s) 312, 437, 528, 656
BslI CCNNNNNNNGG 2 cut(s) 300, 646
BsmAI GTCTC 2 cut(s) 434, 438
BsnI GGCC 2 cut(s) 227, 383
BsoBI CYCGRG 3 cut(s) 116, 527, 607
Bsp1286I GDGCHC 2 cut(s) 117, 174
Bsp13I TCCGGA 2 cut(s) 436, 655
Bsp1407I TGTACA 1 cut(s) 450
Bsp143I GATC 2 cut(s) 60, 603
BspANI GGCC 2 cut(s) 227, 383
BspEI TCCGGA 2 cut(s) 436, 655
BspHI TCATGA 1 cut(s) 547
BspLI GGNNCC 1 cut(s) 585
BspQI GCTCTTC 1 cut(s) 669
BsrFI RCCGGY 1 cut(s) 311
BsrGI TGTACA 1 cut(s) 450
BsrI ACTGG 1 cut(s) 471
BssAI RCCGGY 1 cut(s) 311
BssECI CCNNGG 4 cut(s) 228, 526, 527, 645
BssMI GATC 2 cut(s) 60, 603
BssT1I CCWWGG 2 cut(s) 228, 645
Bst4CI ACNGT 3 cut(s) 337, 445, 545
Bst6I CTCTTC 3 cut(s) 177, 204, 669
BstAUI TGTACA 1 cut(s) 450
BstEII GGTNACC 1 cut(s) 331
BstENI CCTNNNNNAGG 1 cut(s) 644
BstF5I GGATG 1 cut(s) 688
BstH2I RGCGCY 1 cut(s) 526
BstHHI GCGC 1 cut(s) 525
BstKTI GATC 2 cut(s) 63, 606
BstMAI GTCTC 2 cut(s) 434, 438
BstMBI GATC 2 cut(s) 60, 603
BstPI GGTNACC 1 cut(s) 331
BstSCI CCNGG 2 cut(s) 526, 527
BstSFI CTRYAG 1 cut(s) 541
BsuRI GGCC 2 cut(s) 227, 383
BtsCI GGATG 1 cut(s) 688
BtsIMutI CAGTG 1 cut(s) 478
CciI TCATGA 1 cut(s) 547
CfoI GCGC 1 cut(s) 525
Cfr10I RCCGGY 1 cut(s) 311
Cfr9I CCCGGG 1 cut(s) 527
Csp6I GTAC 3 cut(s) 307, 427, 451
CspAI ACCGGT 1 cut(s) 311
CspCI CAANNNNNGTGG 2 cut(s) 389, 424
CviAII CATG 5 cut(s) 192, 240, 278, 424, 548
CviQI GTAC 3 cut(s) 307, 427, 451
DpnI GATC 2 cut(s) 62, 605
DpnII GATC 2 cut(s) 60, 603
DraIII CACNNNGTG 1 cut(s) 355
EaeI YGGCCR 1 cut(s) 381
Eam1104I CTCTTC 3 cut(s) 177, 204, 669
EarI CTCTTC 3 cut(s) 177, 204, 669
Ecl136II GAGCTC 1 cut(s) 115
Eco130I CCWWGG 2 cut(s) 228, 645
Eco24I GRGCYC 1 cut(s) 117
Eco53kI GAGCTC 1 cut(s) 115
Eco57I CTGAAG 1 cut(s) 693
Eco88I CYCGRG 3 cut(s) 116, 527, 607
Eco91I GGTNACC 1 cut(s) 331
EcoICRI GAGCTC 1 cut(s) 115
EcoNI CCTNNNNNAGG 1 cut(s) 644
EcoO65I GGTNACC 1 cut(s) 331
EcoT14I CCWWGG 2 cut(s) 228, 645
EcoT38I GRGCYC 1 cut(s) 117
ErhI CCWWGG 2 cut(s) 228, 645
FaeI CATG 5 cut(s) 195, 243, 281, 427, 551
FaiI YATR 8 cut(s) 177, 193, 219, 241, 279, 347, 425, 549
FatI CATG 5 cut(s) 191, 239, 277, 423, 547
FokI GGATG 1 cut(s) 675
FriOI GRGCYC 1 cut(s) 117
FspBI CTAG 3 cut(s) 618, 629, 641
GlaI GCGC 1 cut(s) 524
GsuI CTGGAG 1 cut(s) 537
HaeII RGCGCY 1 cut(s) 526
HaeIII GGCC 2 cut(s) 227, 383
HapII CCGG 4 cut(s) 312, 437, 528, 656
HhaI GCGC 1 cut(s) 525
Hin1II CATG 5 cut(s) 195, 243, 281, 427, 551
Hin6I GCGC 1 cut(s) 523
HinP1I GCGC 1 cut(s) 523
HincII GTYRAC 1 cut(s) 135
HindII GTYRAC 1 cut(s) 135
HinfI GANTC 5 cut(s) 92, 98, 290, 532, 610
HpaII CCGG 4 cut(s) 312, 437, 528, 656
HphI GGTGA 5 cut(s) 191, 199, 326, 343, 380
Hpy166II GTNNAC 3 cut(s) 135, 309, 666
Hpy188I TCNGA 3 cut(s) 65, 97, 295
Hpy188III TCNNGA 9 cut(s) 323, 401, 437, 503, 516, 548, 596, 607, 656
Hpy8I GTNNAC 3 cut(s) 135, 309, 666
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 5 cut(s) 4, 62, 169, 320, 382
HpyCH4III ACNGT 3 cut(s) 337, 445, 545
HpyCH4IV ACGT 1 cut(s) 567
HpyCH4V TGCA 1 cut(s) 239
HpySE526I ACGT 1 cut(s) 567
Hsp92II CATG 5 cut(s) 195, 243, 281, 427, 551
HspAI GCGC 1 cut(s) 523
Kpn2I TCCGGA 2 cut(s) 436, 655
Kzo9I GATC 2 cut(s) 60, 603
LguI GCTCTTC 1 cut(s) 669
LmnI GCTCC 1 cut(s) 583
LpnPI CCDG 9 cut(s) 18, 300, 325, 450, 484, 501, 541, 609, 669
MaeI CTAG 3 cut(s) 618, 629, 641
MaeII ACGT 1 cut(s) 567
MaeIII GTNAC 6 cut(s) 35, 187, 197, 314, 331, 368
MalI GATC 2 cut(s) 62, 605
MboI GATC 2 cut(s) 60, 603
MboII GAAGA 3 cut(s) 194, 221, 686
MhlI GDGCHC 2 cut(s) 117, 174
MlsI TGGCCA 1 cut(s) 383
MluCI AATT 1 cut(s) 591
MluNI TGGCCA 1 cut(s) 383
MlyI GAGTC 1 cut(s) 619
Mox20I TGGCCA 1 cut(s) 383
MroI TCCGGA 2 cut(s) 436, 655
MroXI GAANNNNTTC 2 cut(s) 536, 678
MscI TGGCCA 1 cut(s) 383
MslI CAYNNNNRTG 3 cut(s) 177, 422, 552
Msp20I TGGCCA 1 cut(s) 383
MspA1I CMGCKG 1 cut(s) 671
MspI CCGG 4 cut(s) 312, 437, 528, 656
MspR9I CCNGG 2 cut(s) 528, 529
NciI CCSGG 2 cut(s) 528, 529
NdeII GATC 2 cut(s) 60, 603
NlaIII CATG 5 cut(s) 195, 243, 281, 427, 551
NlaIV GGNNCC 1 cut(s) 585
NmuCI GTSAC 5 cut(s) 187, 197, 314, 331, 368
OliI CACNNNNGTG 1 cut(s) 177
PaeR7I CTCGAG 2 cut(s) 116, 607
PagI TCATGA 1 cut(s) 547
PciSI GCTCTTC 1 cut(s) 669
PdmI GAANNNNTTC 2 cut(s) 536, 678
PfeI GAWTC 4 cut(s) 92, 98, 290, 532
PinAI ACCGGT 1 cut(s) 311
PleI GAGTC 1 cut(s) 618
PpsI GAGTC 1 cut(s) 618
Psp124BI GAGCTC 1 cut(s) 117
PspEI GGTNACC 1 cut(s) 331
PspN4I GGNNCC 1 cut(s) 585
PspXI VCTCGAGB 1 cut(s) 116
PvuII CAGCTG 1 cut(s) 671
RsaI GTAC 3 cut(s) 308, 428, 452
RsaNI GTAC 3 cut(s) 307, 427, 451
RseI CAYNNNNRTG 3 cut(s) 177, 422, 552
SacI GAGCTC 1 cut(s) 117
SapI GCTCTTC 1 cut(s) 669
Sau3AI GATC 2 cut(s) 60, 603
SchI GAGTC 1 cut(s) 619
ScrFI CCNGG 2 cut(s) 528, 529
SduI GDGCHC 2 cut(s) 117, 174
SfcI CTRYAG 1 cut(s) 541
Sfr274I CTCGAG 2 cut(s) 116, 607
SgrAI CRCCGGYG 1 cut(s) 311
SlaI CTCGAG 2 cut(s) 116, 607
SmaI CCCGGG 1 cut(s) 529
SmiMI CAYNNNNRTG 3 cut(s) 177, 422, 552
SmlI CTYRAG 2 cut(s) 116, 607
SmoI CTYRAG 2 cut(s) 116, 607
Sse9I AATT 1 cut(s) 591
SspMI CTAG 3 cut(s) 618, 629, 641
SstI GAGCTC 1 cut(s) 117
StyD4I CCNGG 2 cut(s) 526, 527
StyI CCWWGG 2 cut(s) 228, 645
TaaI ACNGT 3 cut(s) 337, 445, 545
TaiI ACGT 1 cut(s) 570
TaqI TCGA 4 cut(s) 106, 117, 340, 608
TaqII GACCGA 2 cut(s) 73, 263
TasI AATT 1 cut(s) 591
TatI WGTACW 3 cut(s) 306, 426, 450
TfiI GAWTC 4 cut(s) 92, 98, 290, 532
TscAI CASTG 1 cut(s) 478
TseFI GTSAC 5 cut(s) 187, 197, 314, 331, 368
Tsp45I GTSAC 5 cut(s) 187, 197, 314, 331, 368
TspDTI ATGAA 2 cut(s) 17, 151
TspMI CCCGGG 1 cut(s) 527
TspRI CASTG 1 cut(s) 478
XagI CCTNNNNNAGG 1 cut(s) 644
XapI RAATTY 1 cut(s) 591
XhoI CTCGAG 2 cut(s) 116, 607
XmaI CCCGGG 1 cut(s) 527
XmnI GAANNNNTTC 2 cut(s) 536, 678
XspI CTAG 3 cut(s) 618, 629, 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.