FvH4_5g34520

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001294
Physical Location & Seq
Forward (+)
25158683 .. 25159480
798 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g34520.t1

Sequence Viewer

Length: 798 bp
ATGGGAGGCATGAAGAGGAAGGCAGAAGAGAGCTTGGGTTCAGGCCACAAGTGGATGAGGGGAAGCAAGATCGGCGAGGGAGGCTTCGGCTCTGTCTTCGTAGCTTTCACCAGGATACCCTACATCCACAGCCACCACATGCCTGAGGCCATGGCCATGAAATCTGCAAAGATGAAGGACTCGGGTTCTATTCAATACGAGCTCGAGGTATACAACGACATGAAGAATCGCGGCCGTCTTTGCCCTTTCATTATCGACCATTACGGTGAAGAGGTGACAACGAGCAGTAAGGGCGAAGAGGTTTACAACTTGGCGTTGGAAGTCGCGCGGGGAAGTCTTGCAAGGCTTATAAAGAATGTGGGTGGTGGGTTGCCTCAACACGATGTGCGAAGGTATACCCGTGACATTCTTGAAGGGATTGCCTACATCCACAAGTGTGGTTACGTCCACTGTGACTTGAAGCCAGAGAATGTTCTTCTTGTGGAAAATGAAGACGAGGACGTCCCTAGGAGAATAATTGCTAAAGTGGCAGACTTGGGATTGGCCAAGAAGAAGGACGAGGTTCCTGGAAGGTGGAGAGGTACACCCATGTACTTGGCTCCTGAGACTCTACGGTTCGATGAACAAGAGGAGCCAAGTGATATATGGGCCTTGGGGTGCATAGTTCTTGAGATGATCACCGGAGAGCCCGCTCGGAACCACTGTTACGATGAAGAGAGTAACAAGATCGTTGTTGTTGACGGAGTTGTGCCTAAAATCCCTGAAGATGAAATCTCAGGTGTTGCGAGGATTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

266

Amino Acids

29.7

Weight (kDa)

5.77

Isoelectric Point (pI)

46.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 20 - 239 7.2e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 22 - 243 7.8e-20 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 350
AasI GACNNNNNNGTC 1 cut(s) 500
AatII GACGTC 1 cut(s) 504
AccBSI CCGCTC 1 cut(s) 692
AccI GTMKAC 2 cut(s) 210, 395
AccII CGCG 3 cut(s) 231, 326, 328
AciI CCGC 3 cut(s) 231, 328, 690
AcoI YGGCCR 3 cut(s) 153, 232, 543
AcuI CTGAAG 1 cut(s) 783
AcyI GRCGYC 1 cut(s) 501
AdeI CACNNNGTG 1 cut(s) 385
AfaI GTAC 2 cut(s) 583, 593
AgsI TTSAA 3 cut(s) 194, 413, 460
AjnI CCWGG 2 cut(s) 110, 565
AleI CACNNNNGTG 1 cut(s) 435
AluBI AGCT 3 cut(s) 33, 104, 202
AluI AGCT 3 cut(s) 33, 104, 202
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 1 cut(s) 599
Ama87I CYCGRG 2 cut(s) 181, 203
AoxI GGCC 6 cut(s) 43, 147, 153, 232, 543, 648
AspA2I CCTAGG 1 cut(s) 506
AspLEI GCGC 1 cut(s) 328
AspS9I GGNCC 1 cut(s) 648
AsuHPI GGTGA 4 cut(s) 100, 278, 286, 670
AvaI CYCGRG 2 cut(s) 181, 203
AvrII CCTAGG 1 cut(s) 506
AxyI CCTNAGG 1 cut(s) 144
BalI TGGCCA 2 cut(s) 155, 545
BanII GRGCYC 2 cut(s) 204, 690
BbsI GAAGAC 2 cut(s) 88, 498
Bbv12I GWGCWC 1 cut(s) 204
BceAI ACGGC 1 cut(s) 219
BciT130I CCWGG 2 cut(s) 112, 567
BciVI GTATCC 1 cut(s) 108
BclI TGATCA 1 cut(s) 675
BcoDI GTCTC 1 cut(s) 599
BfaI CTAG 2 cut(s) 507, 796
BfuI GTATCC 1 cut(s) 108
BisI GCNGC 1 cut(s) 232
BlnI CCTAGG 1 cut(s) 506
BlsI GCNGC 1 cut(s) 233
Bme1390I CCNGG 2 cut(s) 112, 567
BmeT110I CYCGRG 2 cut(s) 181, 203
BmgT120I GGNCC 1 cut(s) 648
BmiI GGNNCC 4 cut(s) 564, 600, 633, 698
BmrFI CCNGG 2 cut(s) 112, 567
BpiI GAAGAC 2 cut(s) 88, 498
BpuEI CTTGAG 1 cut(s) 689
BsaHI GRCGYC 1 cut(s) 501
BsaJI CCNNGG 3 cut(s) 150, 506, 651
BsaWI WCCGGW 1 cut(s) 680
Bse21I CCTNAGG 1 cut(s) 144
BseBI CCWGG 2 cut(s) 112, 567
BseDI CCNNGG 3 cut(s) 150, 506, 651
BseGI GGATG 3 cut(s) 60, 123, 426
BseMII CTCAG 3 cut(s) 135, 594, 789
BseRI GAGGAG 1 cut(s) 644
BseX3I CGGCCG 1 cut(s) 232
Bsh1236I CGCG 3 cut(s) 231, 326, 328
Bsh1285I CGRYCG 1 cut(s) 235
BshFI GGCC 6 cut(s) 45, 149, 155, 234, 545, 650
BsiEI CGRYCG 1 cut(s) 235
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 2 cut(s) 181, 203
BsiSI CCGG 1 cut(s) 681
BslFI GGGAC 1 cut(s) 488
BsmAI GTCTC 1 cut(s) 599
BsmFI GGGAC 1 cut(s) 488
BsnI GGCC 6 cut(s) 45, 149, 155, 234, 545, 650
BsoBI CYCGRG 2 cut(s) 181, 203
Bsp1286I GDGCHC 2 cut(s) 204, 690
Bsp143I GATC 3 cut(s) 69, 675, 726
Bsp19I CCATGG 1 cut(s) 150
BspACI CCGC 3 cut(s) 231, 328, 690
BspANI GGCC 6 cut(s) 45, 149, 155, 234, 545, 650
BspCNI CTCAG 3 cut(s) 136, 595, 788
BspFNI CGCG 3 cut(s) 231, 326, 328
BspLI GGNNCC 4 cut(s) 564, 600, 633, 698
BsrBI CCGCTC 1 cut(s) 692
BssECI CCNNGG 3 cut(s) 150, 506, 651
BssMI GATC 3 cut(s) 69, 675, 726
BssNAI GTATAC 2 cut(s) 211, 396
BssNI GRCGYC 1 cut(s) 501
BssT1I CCWWGG 3 cut(s) 150, 506, 651
Bst1107I GTATAC 2 cut(s) 211, 396
Bst2UI CCWGG 2 cut(s) 112, 567
Bst4CI ACNGT 4 cut(s) 266, 452, 615, 704
Bst6I CTCTTC 5 cut(s) 8, 21, 264, 291, 708
BstACI GRCGYC 1 cut(s) 501
BstC8I GCNNGC 1 cut(s) 690
BstDEI CTNAG 3 cut(s) 144, 603, 775
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 3 cut(s) 60, 123, 426
BstFNI CGCG 3 cut(s) 231, 326, 328
BstHHI GCGC 1 cut(s) 328
BstKTI GATC 3 cut(s) 72, 678, 729
BstMAI GTCTC 1 cut(s) 599
BstMBI GATC 3 cut(s) 69, 675, 726
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 5 cut(s) 72, 81, 240, 291, 527
BstNI CCWGG 2 cut(s) 112, 567
BstNSI RCATGY 1 cut(s) 142
BstSCI CCNGG 2 cut(s) 110, 565
BstUI CGCG 3 cut(s) 231, 326, 328
BstV2I GAAGAC 2 cut(s) 88, 498
BstXI CCANNNNNNTGG 2 cut(s) 437, 595
BstZ17I GTATAC 2 cut(s) 211, 396
BstZI CGGCCG 1 cut(s) 232
Bsu36I CCTNAGG 1 cut(s) 144
BsuI GTATCC 1 cut(s) 108
BsuRI GGCC 6 cut(s) 45, 149, 155, 234, 545, 650
BtgI CCRYGG 1 cut(s) 150
BtsCI GGATG 3 cut(s) 60, 123, 426
BtsIMutI CAGTG 2 cut(s) 448, 700
Cac8I GCNNGC 1 cut(s) 690
CfoI GCGC 1 cut(s) 328
Cfr13I GGNCC 1 cut(s) 648
Csp6I GTAC 2 cut(s) 582, 592
CviAII CATG 6 cut(s) 10, 139, 151, 157, 220, 589
CviQI GTAC 2 cut(s) 582, 592
DdeI CTNAG 3 cut(s) 144, 603, 775
DpnI GATC 3 cut(s) 71, 677, 728
DpnII GATC 3 cut(s) 69, 675, 726
DraIII CACNNNGTG 1 cut(s) 385
DrdI GACNNNNNNGTC 1 cut(s) 500
DseDI GACNNNNNNGTC 1 cut(s) 500
EaeI YGGCCR 3 cut(s) 153, 232, 543
EagI CGGCCG 1 cut(s) 232
Eam1104I CTCTTC 5 cut(s) 8, 21, 264, 291, 708
EarI CTCTTC 5 cut(s) 8, 21, 264, 291, 708
Ecl136II GAGCTC 1 cut(s) 202
EclXI CGGCCG 1 cut(s) 232
Eco130I CCWWGG 3 cut(s) 150, 506, 651
Eco24I GRGCYC 2 cut(s) 204, 690
Eco52I CGGCCG 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 202
Eco57I CTGAAG 1 cut(s) 783
Eco81I CCTNAGG 1 cut(s) 144
Eco88I CYCGRG 2 cut(s) 181, 203
EcoICRI GAGCTC 1 cut(s) 202
EcoRII CCWGG 2 cut(s) 110, 565
EcoT14I CCWWGG 3 cut(s) 150, 506, 651
EcoT38I GRGCYC 2 cut(s) 204, 690
ErhI CCWWGG 3 cut(s) 150, 506, 651
FaeI CATG 6 cut(s) 13, 142, 154, 160, 223, 592
FaqI GGGAC 1 cut(s) 488
FatI CATG 6 cut(s) 9, 138, 150, 156, 219, 588
FauI CCCGC 2 cut(s) 321, 697
FbaI TGATCA 1 cut(s) 675
FblI GTMKAC 2 cut(s) 210, 395
Fnu4HI GCNGC 1 cut(s) 232
FokI GGATG 3 cut(s) 67, 110, 413
FriOI GRGCYC 2 cut(s) 204, 690
Fsp4HI GCNGC 1 cut(s) 232
FspBI CTAG 2 cut(s) 507, 796
GlaI GCGC 1 cut(s) 327
GluI GCNGC 1 cut(s) 232
HaeIII GGCC 6 cut(s) 45, 149, 155, 234, 545, 650
HapII CCGG 1 cut(s) 681
HhaI GCGC 1 cut(s) 328
Hin1I GRCGYC 1 cut(s) 501
Hin1II CATG 6 cut(s) 13, 142, 154, 160, 223, 592
Hin6I GCGC 1 cut(s) 326
HinP1I GCGC 1 cut(s) 326
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HinfI GANTC 3 cut(s) 179, 226, 607
HpaII CCGG 1 cut(s) 681
HphI GGTGA 4 cut(s) 100, 278, 286, 670
Hpy166II GTNNAC 6 cut(s) 211, 304, 396, 448, 584, 739
Hpy188I TCNGA 1 cut(s) 696
Hpy188III TCNNGA 3 cut(s) 410, 602, 668
Hpy8I GTNNAC 6 cut(s) 211, 304, 396, 448, 584, 739
HpyAV CCTTC 6 cut(s) 13, 169, 384, 407, 547, 564
HpyCH4III ACNGT 4 cut(s) 266, 452, 615, 704
HpyCH4IV ACGT 2 cut(s) 444, 501
HpyCH4V TGCA 3 cut(s) 167, 341, 660
HpyF10VI GCNNNNNNNGC 5 cut(s) 72, 81, 240, 291, 527
HpyF3I CTNAG 3 cut(s) 144, 603, 775
HpySE526I ACGT 2 cut(s) 444, 501
Hsp92I GRCGYC 1 cut(s) 501
Hsp92II CATG 6 cut(s) 13, 142, 154, 160, 223, 592
HspAI GCGC 1 cut(s) 326
Ksp22I TGATCA 1 cut(s) 675
Kzo9I GATC 3 cut(s) 69, 675, 726
LmnI GCTCC 2 cut(s) 604, 631
MaeI CTAG 2 cut(s) 507, 796
MaeII ACGT 2 cut(s) 444, 501
MaeIII GTNAC 6 cut(s) 274, 401, 440, 452, 704, 719
MalI GATC 3 cut(s) 71, 677, 728
MbiI CCGCTC 1 cut(s) 692
MboI GATC 3 cut(s) 69, 675, 726
MhlI GDGCHC 2 cut(s) 204, 690
MlsI TGGCCA 2 cut(s) 155, 545
MluCI AATT 1 cut(s) 516
MluNI TGGCCA 2 cut(s) 155, 545
MlyI GAGTC 2 cut(s) 173, 601
MmeI TCCRAC 1 cut(s) 297
Mox20I TGGCCA 2 cut(s) 155, 545
MscI TGGCCA 2 cut(s) 155, 545
MslI CAYNNNNRTG 3 cut(s) 155, 264, 435
Msp20I TGGCCA 2 cut(s) 155, 545
MspI CCGG 1 cut(s) 681
MspR9I CCNGG 2 cut(s) 112, 567
MvaI CCWGG 2 cut(s) 112, 567
MvnI CGCG 3 cut(s) 231, 326, 328
MwoI GCNNNNNNNGC 5 cut(s) 72, 81, 240, 291, 527
NcoI CCATGG 1 cut(s) 150
NdeII GATC 3 cut(s) 69, 675, 726
NlaIII CATG 6 cut(s) 13, 142, 154, 160, 223, 592
NlaIV GGNNCC 4 cut(s) 564, 600, 633, 698
NmuCI GTSAC 3 cut(s) 274, 401, 452
NspI RCATGY 1 cut(s) 142
OliI CACNNNNGTG 1 cut(s) 435
PaeR7I CTCGAG 1 cut(s) 203
PfeI GAWTC 1 cut(s) 226
PfoI TCCNGGA 1 cut(s) 565
PkrI GCNGC 1 cut(s) 233
PleI GAGTC 2 cut(s) 173, 601
PpsI GAGTC 2 cut(s) 173, 601
PsiI TTATAA 1 cut(s) 350
Psp124BI GAGCTC 1 cut(s) 204
Psp6I CCWGG 2 cut(s) 110, 565
PspGI CCWGG 2 cut(s) 110, 565
PspN4I GGNNCC 4 cut(s) 564, 600, 633, 698
PspPI GGNCC 1 cut(s) 648
PspXI VCTCGAGB 1 cut(s) 203
PsrI GAACNNNNNNTAC 2 cut(s) 689, 721
RsaI GTAC 2 cut(s) 583, 593
RsaNI GTAC 2 cut(s) 582, 592
RseI CAYNNNNRTG 3 cut(s) 155, 264, 435
SacI GAGCTC 1 cut(s) 204
SatI GCNGC 1 cut(s) 232
Sau3AI GATC 3 cut(s) 69, 675, 726
Sau96I GGNCC 1 cut(s) 648
SchI GAGTC 2 cut(s) 173, 601
ScrFI CCNGG 2 cut(s) 112, 567
SduI GDGCHC 2 cut(s) 204, 690
Sfr274I CTCGAG 1 cut(s) 203
SlaI CTCGAG 1 cut(s) 203
SmiMI CAYNNNNRTG 3 cut(s) 155, 264, 435
SmlI CTYRAG 2 cut(s) 203, 668
SmoI CTYRAG 2 cut(s) 203, 668
Sse9I AATT 1 cut(s) 516
SsiI CCGC 3 cut(s) 231, 328, 690
SspMI CTAG 2 cut(s) 507, 796
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 2 cut(s) 110, 565
StyI CCWWGG 3 cut(s) 150, 506, 651
TaaI ACNGT 4 cut(s) 266, 452, 615, 704
TaiI ACGT 2 cut(s) 447, 504
TaqI TCGA 3 cut(s) 204, 255, 618
TasI AATT 1 cut(s) 516
TatI WGTACW 1 cut(s) 591
TauI GCSGC 1 cut(s) 234
TfiI GAWTC 1 cut(s) 226
TscAI CASTG 2 cut(s) 455, 707
TseFI GTSAC 3 cut(s) 274, 401, 452
Tsp45I GTSAC 3 cut(s) 274, 401, 452
TspDTI ATGAA 9 cut(s) 26, 173, 188, 236, 238, 504, 636, 726, 783
TspGWI ACGGA 1 cut(s) 756
TspRI CASTG 2 cut(s) 455, 707
XceI RCATGY 1 cut(s) 142
XcmI CCANNNNNNNNNTGG 1 cut(s) 642
XhoI CTCGAG 1 cut(s) 203
XmaJI CCTAGG 1 cut(s) 506
XmiI GTMKAC 2 cut(s) 210, 395
XspI CTAG 2 cut(s) 507, 796
ZraI GACGTC 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.