pycom09g14640

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0001294
Physical Location & Seq
Forward (+)
14346933 .. 14347972
1040 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g14640.3

Sequence Viewer

Length: 840 bp
ATGGTGAAAAGAAAAGCCGAAGAAGAAACTCGGAAAATGTGGAATCATGGAGAGCATAAACGTAAAAGGGGTCTGTTGACTGGCAATTTTCCTGCATTGATGGCCGTGAAATCGGCAGAGCTCTTTGCTTCAGAGTCCATTCAGCACGAGGCAGAGGTTCTCTTTGAGATTAAGAGCTGCCCTTTTATTATCGAGCGCTTTGGCAAAGAGACCACGACAACCACCGACAAGGGTGACAAGGTGTGCAAATTACTGTTAGAATATGCCGCTGGAGGAACCCTTGATGGTCTCATCCAAAGATCCAACGCGAAGATTGCAGATTTTGGATTGTCTAAGAGAAGCAAGGAGAAATACAGTGGATGGAGAGGCACTCCTAGGAATCTATCCCCAGAAGCTTTAAAAGTTATGAAGCAAGATGAGTCCTCTGATATATGGTCTCTTGGTTGTATAGTGTTTGAGATGCTAGTGGGCATGTCGCCCTGGGAGTTGAAGCCAGGTTGTAGTGACCGTGTCGATATCCTCGATGTGACAATGTTGGATCATCTGCGTACTTCAAGAATTCCTGCTGAAATCTCATATGTGGCCGGGGATTTTCTAGAGATTTGCCTTGCCATGAGACCTGAGAAAAGATTAACTGCTGAGGAGCTCCTGTCTCATCCGTTCGTAGTGCAGCCGCAACCAAAAAAACCAGGTCATGTCAGGGTAAGGGTGGTTAACTCAGCCTTAGGTCAAGCATATGGCATCTGTTTCTTAAAACCGAGTGCAAAATACCACGCAAGCGTTGCGATTGTTTCTAGAATTCAGCCTACCCCGGGTTTTGGTATACGATCTGGAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

280

Amino Acids

31.1

Weight (kDa)

9.15

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 823
AccII CGCG 1 cut(s) 308
AciI CCGC 2 cut(s) 267, 674
AclWI GGATC 2 cut(s) 294, 546
AcoI YGGCCR 2 cut(s) 102, 582
AcsI RAATTY 2 cut(s) 558, 798
AcuI CTGAAG 1 cut(s) 114
AfaI GTAC 1 cut(s) 550
AfeI AGCGCT 1 cut(s) 197
AfiI CCNNNNNNNGG 2 cut(s) 812, 818
AgsI TTSAA 2 cut(s) 490, 555
AjnI CCWGG 3 cut(s) 479, 493, 688
AluBI AGCT 4 cut(s) 121, 177, 395, 646
AluI AGCT 4 cut(s) 121, 177, 395, 646
Alw21I GWGCWC 2 cut(s) 123, 648
Alw26I GTCTC 5 cut(s) 203, 293, 441, 610, 657
AlwI GGATC 2 cut(s) 294, 546
Ama87I CYCGRG 1 cut(s) 811
Aor51HI AGCGCT 1 cut(s) 197
AoxI GGCC 2 cut(s) 102, 582
ApeKI GCWGC 2 cut(s) 177, 670
ApoI RAATTY 2 cut(s) 558, 798
AspA2I CCTAGG 1 cut(s) 374
AspLEI GCGC 1 cut(s) 198
AsuC2I CCSGG 3 cut(s) 586, 812, 813
AsuHPI GGTGA 2 cut(s) 16, 245
AvaI CYCGRG 1 cut(s) 811
AvrII CCTAGG 1 cut(s) 374
AxyI CCTNAGG 1 cut(s) 724
BanII GRGCYC 2 cut(s) 123, 648
BauI CACGAG 1 cut(s) 146
Bbv12I GWGCWC 2 cut(s) 123, 648
BbvCI CCTCAGC 1 cut(s) 639
BbvI GCAGC 2 cut(s) 164, 682
BccI CCATC 3 cut(s) 94, 278, 354
BceAI ACGGC 1 cut(s) 89
BciT130I CCWGG 3 cut(s) 481, 495, 690
BcnI CCSGG 3 cut(s) 586, 812, 813
BcoDI GTCTC 5 cut(s) 203, 293, 441, 610, 657
BfaI CTAG 4 cut(s) 375, 464, 596, 795
BfoI RGCGCY 1 cut(s) 199
BisI GCNGC 4 cut(s) 178, 267, 671, 674
BlnI CCTAGG 1 cut(s) 374
BlsI GCNGC 4 cut(s) 179, 268, 672, 675
Bme1390I CCNGG 6 cut(s) 481, 495, 586, 690, 812, 813
BmeT110I CYCGRG 1 cut(s) 811
BmiI GGNNCC 1 cut(s) 277
BmrFI CCNGG 6 cut(s) 481, 495, 586, 690, 812, 813
BmsI GCATC 2 cut(s) 450, 750
BplI GAGNNNNNCTC 2 cut(s) 355, 387
BpmI CTGGAG 1 cut(s) 291
Bpu10I CCTNAGC 1 cut(s) 639
BpuMI CCSGG 3 cut(s) 586, 812, 813
BsaI GGTCTC 4 cut(s) 203, 293, 441, 610
BsaJI CCNNGG 6 cut(s) 374, 479, 480, 585, 810, 811
BsaXI ACNNNNNCTCC 2 cut(s) 635, 665
Bsc4I CCNNNNNNNGG 2 cut(s) 812, 818
Bse1I ACTGG 1 cut(s) 85
Bse21I CCTNAGG 1 cut(s) 724
BseBI CCWGG 3 cut(s) 481, 495, 690
BseDI CCNNGG 6 cut(s) 374, 479, 480, 585, 810, 811
BseGI GGATG 3 cut(s) 291, 365, 655
BseLI CCNNNNNNNGG 2 cut(s) 812, 818
BseMII CTCAG 3 cut(s) 612, 630, 732
BseNI ACTGG 1 cut(s) 85
BseRI GAGGAG 1 cut(s) 656
BseXI GCAGC 2 cut(s) 164, 682
BsgI GTGCAG 1 cut(s) 689
Bsh1236I CGCG 1 cut(s) 308
BshFI GGCC 2 cut(s) 104, 584
BsiHKAI GWGCWC 2 cut(s) 123, 648
BsiHKCI CYCGRG 1 cut(s) 811
BsiSI CCGG 2 cut(s) 585, 812
BslI CCNNNNNNNGG 2 cut(s) 812, 818
BsmAI GTCTC 5 cut(s) 203, 293, 441, 610, 657
BsnI GGCC 2 cut(s) 104, 584
Bso31I GGTCTC 4 cut(s) 203, 293, 441, 610
BsoBI CYCGRG 1 cut(s) 811
Bsp1286I GDGCHC 2 cut(s) 123, 648
Bsp143I GATC 3 cut(s) 299, 538, 827
BspACI CCGC 2 cut(s) 267, 674
BspANI GGCC 2 cut(s) 104, 584
BspCNI CTCAG 3 cut(s) 613, 631, 731
BspFNI CGCG 1 cut(s) 308
BspLI GGNNCC 1 cut(s) 277
BspPI GGATC 2 cut(s) 294, 546
BspTNI GGTCTC 4 cut(s) 203, 293, 441, 610
BsrI ACTGG 1 cut(s) 85
BssECI CCNNGG 6 cut(s) 374, 479, 480, 585, 810, 811
BssMI GATC 3 cut(s) 299, 538, 827
BssNAI GTATAC 1 cut(s) 824
BssSI CACGAG 1 cut(s) 146
BssT1I CCWWGG 1 cut(s) 374
Bst1107I GTATAC 1 cut(s) 824
Bst2BI CACGAG 1 cut(s) 146
Bst2UI CCWGG 3 cut(s) 481, 495, 690
Bst4CI ACNGT 3 cut(s) 255, 356, 509
BstAPI GCANNNNNTGC 1 cut(s) 782
BstC8I GCNNGC 1 cut(s) 778
BstDEI CTNAG 5 cut(s) 333, 621, 639, 718, 724
BstF5I GGATG 3 cut(s) 291, 365, 655
BstFNI CGCG 1 cut(s) 308
BstH2I RGCGCY 1 cut(s) 199
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 3 cut(s) 302, 541, 830
BstMAI GTCTC 5 cut(s) 203, 293, 441, 610, 657
BstMBI GATC 3 cut(s) 299, 538, 827
BstMWI GCNNNNNNNGC 3 cut(s) 101, 314, 782
BstNI CCWGG 3 cut(s) 481, 495, 690
BstNSI RCATGY 1 cut(s) 475
BstSCI CCNGG 6 cut(s) 479, 493, 584, 688, 810, 811
BstUI CGCG 1 cut(s) 308
BstV1I GCAGC 2 cut(s) 164, 682
BstX2I RGATCY 1 cut(s) 299
BstYI RGATCY 1 cut(s) 299
BstZ17I GTATAC 1 cut(s) 824
Bsu36I CCTNAGG 1 cut(s) 724
BsuRI GGCC 2 cut(s) 104, 584
BtsCI GGATG 3 cut(s) 291, 365, 655
BtsIMutI CAGTG 1 cut(s) 361
Cac8I GCNNGC 1 cut(s) 778
CfoI GCGC 1 cut(s) 198
Cfr9I CCCGGG 1 cut(s) 811
CsiI ACCWGGT 1 cut(s) 688
Csp6I GTAC 1 cut(s) 549
CviAII CATG 4 cut(s) 47, 472, 613, 695
CviQI GTAC 1 cut(s) 549
DdeI CTNAG 5 cut(s) 333, 621, 639, 718, 724
DpnI GATC 3 cut(s) 301, 540, 829
DpnII GATC 3 cut(s) 299, 538, 827
DraI TTTAAA 1 cut(s) 399
EaeI YGGCCR 2 cut(s) 102, 582
Ecl136II GAGCTC 2 cut(s) 121, 646
Eco130I CCWWGG 1 cut(s) 374
Eco24I GRGCYC 2 cut(s) 123, 648
Eco31I GGTCTC 4 cut(s) 203, 293, 441, 610
Eco32I GATATC 1 cut(s) 517
Eco47III AGCGCT 1 cut(s) 197
Eco53kI GAGCTC 2 cut(s) 121, 646
Eco57I CTGAAG 1 cut(s) 114
Eco81I CCTNAGG 1 cut(s) 724
Eco88I CYCGRG 1 cut(s) 811
EcoICRI GAGCTC 2 cut(s) 121, 646
EcoRI GAATTC 2 cut(s) 558, 798
EcoRII CCWGG 3 cut(s) 479, 493, 688
EcoRV GATATC 1 cut(s) 517
EcoT14I CCWWGG 1 cut(s) 374
EcoT38I GRGCYC 2 cut(s) 123, 648
ErhI CCWWGG 1 cut(s) 374
FaeI CATG 4 cut(s) 50, 475, 616, 698
FatI CATG 4 cut(s) 46, 471, 612, 694
FauNDI CATATG 2 cut(s) 577, 736
FblI GTMKAC 1 cut(s) 823
Fnu4HI GCNGC 4 cut(s) 178, 267, 671, 674
FokI GGATG 3 cut(s) 278, 372, 642
FriOI GRGCYC 2 cut(s) 123, 648
Fsp4HI GCNGC 4 cut(s) 178, 267, 671, 674
FspBI CTAG 4 cut(s) 375, 464, 596, 795
GlaI GCGC 1 cut(s) 197
GluI GCNGC 4 cut(s) 178, 267, 671, 674
GsuI CTGGAG 1 cut(s) 291
HaeII RGCGCY 1 cut(s) 199
HaeIII GGCC 2 cut(s) 104, 584
HapII CCGG 2 cut(s) 585, 812
HhaI GCGC 1 cut(s) 198
Hin1II CATG 4 cut(s) 50, 475, 616, 698
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HincII GTYRAC 2 cut(s) 78, 715
HindII GTYRAC 2 cut(s) 78, 715
HindIII AAGCTT 1 cut(s) 393
HinfI GANTC 4 cut(s) 43, 134, 379, 419
HpaI GTTAAC 1 cut(s) 715
HpaII CCGG 2 cut(s) 585, 812
HphI GGTGA 2 cut(s) 16, 245
Hpy166II GTNNAC 3 cut(s) 78, 715, 824
Hpy188I TCNGA 3 cut(s) 33, 133, 427
Hpy188III TCNNGA 4 cut(s) 555, 596, 795, 831
Hpy8I GTNNAC 3 cut(s) 78, 715, 824
HpyCH4III ACNGT 3 cut(s) 255, 356, 509
HpyCH4IV ACGT 1 cut(s) 61
HpyCH4V TGCA 5 cut(s) 95, 246, 317, 670, 764
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 314, 782
HpyF3I CTNAG 5 cut(s) 333, 621, 639, 718, 724
HpySE526I ACGT 1 cut(s) 61
Hsp92II CATG 4 cut(s) 50, 475, 616, 698
HspAI GCGC 1 cut(s) 196
KspAI GTTAAC 1 cut(s) 715
Kzo9I GATC 3 cut(s) 299, 538, 827
LmnI GCTCC 2 cut(s) 643, 651
Lsp1109I GCAGC 2 cut(s) 164, 682
LweI GCATC 2 cut(s) 450, 750
MabI ACCWGGT 1 cut(s) 688
MaeI CTAG 4 cut(s) 375, 464, 596, 795
MaeII ACGT 1 cut(s) 61
MaeIII GTNAC 3 cut(s) 233, 503, 526
MalI GATC 3 cut(s) 301, 540, 829
MboI GATC 3 cut(s) 299, 538, 827
MboII GAAGA 3 cut(s) 32, 35, 322
MflI RGATCY 1 cut(s) 299
MhlI GDGCHC 2 cut(s) 123, 648
MluCI AATT 4 cut(s) 85, 248, 558, 798
MlyI GAGTC 2 cut(s) 143, 428
MmeI TCCRAC 2 cut(s) 327, 516
MnlI CCTC 7 cut(s) 142, 148, 266, 359, 433, 530, 634
MseI TTAA 5 cut(s) 171, 398, 632, 714, 752
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 2 cut(s) 585, 812
MspR9I CCNGG 6 cut(s) 481, 495, 586, 690, 812, 813
MvaI CCWGG 3 cut(s) 481, 495, 690
MvnI CGCG 1 cut(s) 308
MwoI GCNNNNNNNGC 3 cut(s) 101, 314, 782
NciI CCSGG 3 cut(s) 586, 812, 813
NdeI CATATG 2 cut(s) 577, 736
NdeII GATC 3 cut(s) 299, 538, 827
NlaIII CATG 4 cut(s) 50, 475, 616, 698
NlaIV GGNNCC 1 cut(s) 277
NmuCI GTSAC 3 cut(s) 233, 503, 526
NspI RCATGY 1 cut(s) 475
PasI CCCWGGG 1 cut(s) 480
PcsI WCGNNNNNNNCGW 1 cut(s) 519
PfeI GAWTC 2 cut(s) 43, 379
PflFI GACNNNGTC 1 cut(s) 509
PkrI GCNGC 4 cut(s) 179, 268, 672, 675
PleI GAGTC 2 cut(s) 142, 427
PpsI GAGTC 2 cut(s) 142, 427
Psp124BI GAGCTC 2 cut(s) 123, 648
Psp6I CCWGG 3 cut(s) 479, 493, 688
PspGI CCWGG 3 cut(s) 479, 493, 688
PspN4I GGNNCC 1 cut(s) 277
PsuI RGATCY 1 cut(s) 299
PsyI GACNNNGTC 1 cut(s) 509
RsaI GTAC 1 cut(s) 550
RsaNI GTAC 1 cut(s) 549
SacI GAGCTC 2 cut(s) 123, 648
SaqAI TTAA 5 cut(s) 171, 398, 632, 714, 752
SatI GCNGC 4 cut(s) 178, 267, 671, 674
Sau3AI GATC 3 cut(s) 299, 538, 827
SchI GAGTC 2 cut(s) 143, 428
ScrFI CCNGG 6 cut(s) 481, 495, 586, 690, 812, 813
SduI GDGCHC 2 cut(s) 123, 648
SexAI ACCWGGT 1 cut(s) 688
SfaNI GCATC 2 cut(s) 450, 750
SmaI CCCGGG 1 cut(s) 813
Sse9I AATT 4 cut(s) 85, 248, 558, 798
SsiI CCGC 2 cut(s) 267, 674
SspMI CTAG 4 cut(s) 375, 464, 596, 795
SstI GAGCTC 2 cut(s) 123, 648
StyD4I CCNGG 6 cut(s) 479, 493, 584, 688, 810, 811
StyI CCWWGG 1 cut(s) 374
TaaI ACNGT 3 cut(s) 255, 356, 509
TaiI ACGT 1 cut(s) 64
TaqI TCGA 3 cut(s) 192, 513, 522
TasI AATT 4 cut(s) 85, 248, 558, 798
TauI GCSGC 2 cut(s) 269, 676
TfiI GAWTC 2 cut(s) 43, 379
Tru1I TTAA 5 cut(s) 171, 398, 632, 714, 752
Tru9I TTAA 5 cut(s) 171, 398, 632, 714, 752
TscAI CASTG 1 cut(s) 361
TseFI GTSAC 3 cut(s) 233, 503, 526
TseI GCWGC 2 cut(s) 177, 670
Tsp45I GTSAC 3 cut(s) 233, 503, 526
TspDTI ATGAA 1 cut(s) 422
TspGWI ACGGA 1 cut(s) 648
TspMI CCCGGG 1 cut(s) 811
TspRI CASTG 1 cut(s) 361
Tth111I GACNNNGTC 1 cut(s) 509
XapI RAATTY 2 cut(s) 558, 798
XbaI TCTAGA 2 cut(s) 595, 794
XceI RCATGY 1 cut(s) 475
XmaI CCCGGG 1 cut(s) 811
XmaJI CCTAGG 1 cut(s) 374
XmiI GTMKAC 1 cut(s) 823
XspI CTAG 4 cut(s) 375, 464, 596, 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.