Rroxscaffold_2G00111420

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001294
Physical Location & Seq
Forward (+)
36025406 .. 36026283
878 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00111420.1

Sequence Viewer

Length: 849 bp
ATGAAGAGGAAGGCGGAAGTGAGCTTGGGTTCAGGTCATAAGTGGAAGAGAGGAAAGAAGATCGGCGAAGGAGGCTTCGGTTCTGTGTTCGTTGCATATTCCGAGAGGCCCTATATCATCTCCGATCATATGCCGGAGGCCATGGCTGTGAAATCAGCAAAGATCAAGGATTCCGATTCCATTCGATACGAACTGGAGGTGTTTAGGGAGTTGAATAGCGTGGGCGGTTCTTGCTCCTTCATCATTGAGCACTATGGTGAGGAGGTAACACGGAGTGATGAGGGTGAAGAGATCTATAACTTGGCCTTGGAAGTAGCATGGGGAAGCCTTGATAAGTTGTTAGAGAGTAACTTTCATGGGTTGGAAGAATCCGATGTAAGGCGGTATACCGGTGATATTCTTGAAGGTATTAAACACATACACAAGAGTGGGTATGTCCACTGTGATTTGAAACCAGGTAACATCTTGCTTGTGGAAAATGAAGAAATTGATCGATATCGCTGTACCTATGTGGCCAAAGTTGGTGGCTTGGGGATGGCCAAGAAGGAGGAGGAGGTTCAGGAAGGCTGGAGAGGCACACCCATGTACTTCTCTCCTGAAACTGTCTCCGACAAGAAGCAAGAACAACCCAGTGACATATGGGCTGTGGGGTTGCCCCGGAATCATTTCTATGATGTTGAGAGTGATACTCTCCACATTGTTGATGGAATGGAGCCAAAAATTCCTGATACGATCTCGAGTCTTGCTAAGGAGTTTCTAGAGCTTTGCCTAGCTGAGTTTCCTGAGCAAAGGTGGACAGCTGAAGAACTTCTAACTCATCCCTTCGTGGCAGATGTTGATAGAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

282

Amino Acids

31.99

Weight (kDa)

5.0

Isoelectric Point (pI)

48.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 16 - 276 3.3e-34 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 17 - 271 2.8e-17 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 386
AciI CCGC 3 cut(s) 14, 225, 382
AcoI YGGCCR 2 cut(s) 513, 537
AcsI RAATTY 1 cut(s) 720
AcuI CTGAAG 1 cut(s) 822
AdeI CACNNNGTG 1 cut(s) 275
AfaI GTAC 2 cut(s) 505, 587
AfiI CCNNNNNNNGG 1 cut(s) 378
AgeI ACCGGT 1 cut(s) 389
AgsI TTSAA 3 cut(s) 214, 404, 451
AjnI CCWGG 1 cut(s) 454
AleI CACNNNNGTG 2 cut(s) 255, 426
AluBI AGCT 4 cut(s) 24, 763, 773, 800
AluI AGCT 4 cut(s) 24, 763, 773, 800
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 1 cut(s) 610
Ama87I CYCGRG 1 cut(s) 736
AoxI GGCC 5 cut(s) 107, 138, 303, 513, 537
ApoI RAATTY 1 cut(s) 720
AsiGI ACCGGT 1 cut(s) 389
Asp700I GAANNNNTTC 2 cut(s) 665, 807
AspS9I GGNCC 1 cut(s) 108
AsuC2I CCSGG 1 cut(s) 658
AsuHPI GGTGA 3 cut(s) 269, 296, 404
AvaI CYCGRG 1 cut(s) 736
BalI TGGCCA 2 cut(s) 515, 539
Bbv12I GWGCWC 1 cut(s) 252
BccI CCATC 2 cut(s) 529, 698
BciT130I CCWGG 1 cut(s) 456
BcnI CCSGG 1 cut(s) 658
BcoDI GTCTC 1 cut(s) 610
BfaI CTAG 2 cut(s) 758, 770
BglII AGATCT 1 cut(s) 291
Bme1390I CCNGG 2 cut(s) 456, 658
BmeT110I CYCGRG 1 cut(s) 736
BmgT120I GGNCC 1 cut(s) 108
BmiI GGNNCC 1 cut(s) 714
BmrFI CCNGG 2 cut(s) 456, 658
BmrI ACTGGG 1 cut(s) 624
BmuI ACTGGG 1 cut(s) 624
BplI GAGNNNNNCTC 2 cut(s) 673, 705
BpmI CTGGAG 2 cut(s) 215, 589
Bpu10I CCTNAGC 2 cut(s) 747, 783
BpuMI CCSGG 1 cut(s) 658
Bsa29I ATCGAT 1 cut(s) 493
BsaBI GATNNNNATC 1 cut(s) 495
BsaJI CCNNGG 3 cut(s) 141, 306, 656
BsaWI WCCGGW 1 cut(s) 389
Bsc4I CCNNNNNNNGG 1 cut(s) 378
Bse118I RCCGGY 1 cut(s) 389
Bse1I ACTGG 2 cut(s) 198, 630
Bse8I GATNNNNATC 1 cut(s) 495
BseBI CCWGG 1 cut(s) 456
BseCI ATCGAT 1 cut(s) 493
BseDI CCNNGG 3 cut(s) 141, 306, 656
BseGI GGATG 2 cut(s) 540, 817
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 1 cut(s) 378
BseMII CTCAG 2 cut(s) 765, 774
BseNI ACTGG 2 cut(s) 198, 630
BseRI GAGGAG 3 cut(s) 275, 563, 566
BshFI GGCC 5 cut(s) 109, 140, 305, 515, 539
BshTI ACCGGT 1 cut(s) 389
BshVI ATCGAT 1 cut(s) 493
BsiHKAI GWGCWC 1 cut(s) 252
BsiHKCI CYCGRG 1 cut(s) 736
BsiSI CCGG 3 cut(s) 134, 390, 658
BslI CCNNNNNNNGG 1 cut(s) 378
BsmAI GTCTC 1 cut(s) 610
BsnI GGCC 5 cut(s) 109, 140, 305, 515, 539
BsoBI CYCGRG 1 cut(s) 736
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 6 cut(s) 60, 124, 162, 291, 490, 732
Bsp19I CCATGG 1 cut(s) 141
BspACI CCGC 3 cut(s) 14, 225, 382
BspANI GGCC 5 cut(s) 109, 140, 305, 515, 539
BspCNI CTCAG 2 cut(s) 766, 775
BspDI ATCGAT 1 cut(s) 493
BspLI GGNNCC 1 cut(s) 714
BsrFI RCCGGY 1 cut(s) 389
BsrI ACTGG 2 cut(s) 198, 630
BssAI RCCGGY 1 cut(s) 389
BssECI CCNNGG 3 cut(s) 141, 306, 656
BssMI GATC 6 cut(s) 60, 124, 162, 291, 490, 732
BssNAI GTATAC 1 cut(s) 387
BssT1I CCWWGG 2 cut(s) 141, 306
Bst1107I GTATAC 1 cut(s) 387
Bst2UI CCWGG 1 cut(s) 456
Bst4CI ACNGT 2 cut(s) 443, 604
Bst6I CTCTTC 2 cut(s) 41, 282
BstDEI CTNAG 3 cut(s) 747, 774, 783
BstDSI CCRYGG 1 cut(s) 141
BstF5I GGATG 2 cut(s) 540, 817
BstKTI GATC 6 cut(s) 63, 127, 165, 294, 493, 735
BstMAI GTCTC 1 cut(s) 610
BstMBI GATC 6 cut(s) 60, 124, 162, 291, 490, 732
BstMWI GCNNNNNNNGC 3 cut(s) 72, 231, 573
BstNI CCWGG 1 cut(s) 456
BstSCI CCNGG 2 cut(s) 454, 656
BstX2I RGATCY 1 cut(s) 291
BstYI RGATCY 1 cut(s) 291
BstZ17I GTATAC 1 cut(s) 387
Bsu15I ATCGAT 1 cut(s) 493
BsuRI GGCC 5 cut(s) 109, 140, 305, 515, 539
BsuTUI ATCGAT 1 cut(s) 493
BtgI CCRYGG 1 cut(s) 141
BtsCI GGATG 2 cut(s) 540, 817
BtsIMutI CAGTG 2 cut(s) 439, 637
Cfr10I RCCGGY 1 cut(s) 389
Cfr13I GGNCC 1 cut(s) 108
ClaI ATCGAT 1 cut(s) 493
CsiI ACCWGGT 1 cut(s) 454
Csp6I GTAC 2 cut(s) 504, 586
CspAI ACCGGT 1 cut(s) 389
CspCI CAANNNNNGTGG 2 cut(s) 505, 540
CviAII CATG 4 cut(s) 142, 318, 356, 583
CviQI GTAC 2 cut(s) 504, 586
DdeI CTNAG 3 cut(s) 747, 774, 783
DpnI GATC 6 cut(s) 62, 126, 164, 293, 492, 734
DpnII GATC 6 cut(s) 60, 124, 162, 291, 490, 732
DraIII CACNNNGTG 1 cut(s) 275
EaeI YGGCCR 2 cut(s) 513, 537
Eam1104I CTCTTC 2 cut(s) 41, 282
EarI CTCTTC 2 cut(s) 41, 282
EciI GGCGGA 1 cut(s) 29
Eco130I CCWWGG 2 cut(s) 141, 306
Eco32I GATATC 1 cut(s) 497
Eco57I CTGAAG 1 cut(s) 822
Eco88I CYCGRG 1 cut(s) 736
EcoO109I RGGNCCY 1 cut(s) 108
EcoRII CCWGG 1 cut(s) 454
EcoRV GATATC 1 cut(s) 497
EcoT14I CCWWGG 2 cut(s) 141, 306
ErhI CCWWGG 2 cut(s) 141, 306
FaeI CATG 4 cut(s) 145, 321, 359, 586
FatI CATG 4 cut(s) 141, 317, 355, 582
FauNDI CATATG 2 cut(s) 129, 638
FblI GTMKAC 1 cut(s) 386
FokI GGATG 2 cut(s) 547, 804
FspBI CTAG 2 cut(s) 758, 770
GsuI CTGGAG 2 cut(s) 215, 589
HaeIII GGCC 5 cut(s) 109, 140, 305, 515, 539
HapII CCGG 3 cut(s) 134, 390, 658
Hin1II CATG 4 cut(s) 145, 321, 359, 586
HinfI GANTC 5 cut(s) 170, 176, 368, 661, 739
HpaII CCGG 3 cut(s) 134, 390, 658
HphI GGTGA 3 cut(s) 269, 296, 404
Hpy166II GTNNAC 3 cut(s) 387, 439, 795
Hpy188I TCNGA 5 cut(s) 103, 124, 175, 373, 610
Hpy188III TCNNGA 7 cut(s) 401, 560, 596, 725, 736, 758, 782
Hpy8I GTNNAC 3 cut(s) 387, 439, 795
HpyAV CCTTC 7 cut(s) 4, 62, 247, 398, 538, 557, 832
HpyCH4III ACNGT 2 cut(s) 443, 604
HpyCH4V TGCA 1 cut(s) 95
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 231, 573
HpyF3I CTNAG 3 cut(s) 747, 774, 783
Hsp92II CATG 4 cut(s) 145, 321, 359, 586
Kzo9I GATC 6 cut(s) 60, 124, 162, 291, 490, 732
LmnI GCTCC 2 cut(s) 239, 712
MabI ACCWGGT 1 cut(s) 454
MaeI CTAG 2 cut(s) 758, 770
MaeIII GTNAC 4 cut(s) 265, 347, 458, 632
MalI GATC 6 cut(s) 62, 126, 164, 293, 492, 734
MboI GATC 6 cut(s) 60, 124, 162, 291, 490, 732
MboII GAAGA 7 cut(s) 16, 58, 70, 299, 377, 494, 815
MflI RGATCY 1 cut(s) 291
MhlI GDGCHC 1 cut(s) 252
MlsI TGGCCA 2 cut(s) 515, 539
MluCI AATT 2 cut(s) 486, 720
MluNI TGGCCA 2 cut(s) 515, 539
MlyI GAGTC 1 cut(s) 748
MmeI TCCRAC 2 cut(s) 342, 633
Mox20I TGGCCA 2 cut(s) 515, 539
MroXI GAANNNNTTC 2 cut(s) 665, 807
MscI TGGCCA 2 cut(s) 515, 539
MseI TTAA 1 cut(s) 411
MslI CAYNNNNRTG 5 cut(s) 146, 255, 426, 581, 669
Msp20I TGGCCA 2 cut(s) 515, 539
MspA1I CMGCKG 1 cut(s) 800
MspI CCGG 3 cut(s) 134, 390, 658
MspR9I CCNGG 2 cut(s) 456, 658
MvaI CCWGG 1 cut(s) 456
MwoI GCNNNNNNNGC 3 cut(s) 72, 231, 573
NciI CCSGG 1 cut(s) 658
NcoI CCATGG 1 cut(s) 141
NdeI CATATG 2 cut(s) 129, 638
NdeII GATC 6 cut(s) 60, 124, 162, 291, 490, 732
NlaIII CATG 4 cut(s) 145, 321, 359, 586
NlaIV GGNNCC 1 cut(s) 714
NmuCI GTSAC 1 cut(s) 632
OliI CACNNNNGTG 2 cut(s) 255, 426
PaeR7I CTCGAG 1 cut(s) 736
PdmI GAANNNNTTC 2 cut(s) 665, 807
PfeI GAWTC 4 cut(s) 170, 176, 368, 661
PinAI ACCGGT 1 cut(s) 389
PleI GAGTC 1 cut(s) 747
PpsI GAGTC 1 cut(s) 747
Psp6I CCWGG 1 cut(s) 454
PspGI CCWGG 1 cut(s) 454
PspN4I GGNNCC 1 cut(s) 714
PspPI GGNCC 1 cut(s) 108
PsuI RGATCY 1 cut(s) 291
PvuII CAGCTG 1 cut(s) 800
RsaI GTAC 2 cut(s) 505, 587
RsaNI GTAC 2 cut(s) 504, 586
RseI CAYNNNNRTG 5 cut(s) 146, 255, 426, 581, 669
SaqAI TTAA 1 cut(s) 411
Sau3AI GATC 6 cut(s) 60, 124, 162, 291, 490, 732
Sau96I GGNCC 1 cut(s) 108
SchI GAGTC 1 cut(s) 748
ScrFI CCNGG 2 cut(s) 456, 658
SduI GDGCHC 1 cut(s) 252
SexAI ACCWGGT 1 cut(s) 454
Sfr274I CTCGAG 1 cut(s) 736
SlaI CTCGAG 1 cut(s) 736
SmiMI CAYNNNNRTG 5 cut(s) 146, 255, 426, 581, 669
SmlI CTYRAG 1 cut(s) 736
SmoI CTYRAG 1 cut(s) 736
Sse9I AATT 2 cut(s) 486, 720
SsiI CCGC 3 cut(s) 14, 225, 382
SspMI CTAG 2 cut(s) 758, 770
StyD4I CCNGG 2 cut(s) 454, 656
StyI CCWWGG 2 cut(s) 141, 306
TaaI ACNGT 2 cut(s) 443, 604
TaqI TCGA 3 cut(s) 184, 493, 737
TasI AATT 2 cut(s) 486, 720
TatI WGTACW 1 cut(s) 585
TfiI GAWTC 4 cut(s) 170, 176, 368, 661
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 2 cut(s) 446, 637
TseFI GTSAC 1 cut(s) 632
Tsp45I GTSAC 1 cut(s) 632
TspDTI ATGAA 4 cut(s) 17, 229, 344, 495
TspGWI ACGGA 1 cut(s) 286
TspRI CASTG 2 cut(s) 446, 637
XapI RAATTY 1 cut(s) 720
XbaI TCTAGA 1 cut(s) 757
XcmI CCANNNNNNNNNTGG 1 cut(s) 701
XhoI CTCGAG 1 cut(s) 736
XmiI GTMKAC 1 cut(s) 386
XmnI GAANNNNTTC 2 cut(s) 665, 807
XspI CTAG 2 cut(s) 758, 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.