Rorug01G0258200

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001294
Physical Location & Seq
Reverse (-)
36770828 .. 36771927
1100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0258200.1

Sequence Viewer

Length: 597 bp
ATGGATGAGACACCAAATGATGAAAAAAAATTGGATGCCTCAACTCACCGTAGAAGTGGTGGTTTTATGAAACCTGGCGAGCAGCATGGGAATGGTCACTTGAGAAATAATCATGCACAGGATCCATGGAATTTAGAAGGAAGAGCACTATCTGCATTGAATAGGTTTGGAGAAAAGGCTAGGACTGTTTCAGATCCAGTATGGTTACGTCGGGTTCAACACCAGCATGCCATTATGGAGCCTCCAACTTCAGTGGTTGAGAGGTCAACTCTATCCACCATATTGTCCGAAAAGGGTCTCCATGGGGCTTTGTATGTGGCTGGGGAAGTGCTATTTATAACAAAACCACTTATTTATGTTTTATTTATTCGAAAATATGGAGCTCGCTCCTGGATTCCTTGCTTTCTTTCACTGGCTGTGGACTTCACTGGAATGCACATTCTATCTCGAATTACTTCATACAGAGGTGGTACAAAAGAGCAGCAGTTTCATCTTTCTGTCCCTGAAAAGAATGAGGGTAAAAGACAAAAACTGTTATGGACACTTTACCTCATGAGAGATCCATTTTTCAGCAAGTATACCAGGTTGGTTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

198

Amino Acids

22.72

Weight (kDa)

9.81

Isoelectric Point (pI)

36.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pex16 PF08610 15 - 195 2.8e-34 Peroxisomal membrane protein (Pex16)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 338
AccI GTMKAC 1 cut(s) 578
AclWI GGATC 4 cut(s) 116, 129, 188, 554
AcsI RAATTY 1 cut(s) 130
AcuI CTGAAG 1 cut(s) 234
AfaI GTAC 1 cut(s) 472
AgsI TTSAA 2 cut(s) 160, 218
AjnI CCWGG 3 cut(s) 73, 389, 581
AluBI AGCT 1 cut(s) 383
AluI AGCT 1 cut(s) 383
Alw21I GWGCWC 2 cut(s) 148, 385
Alw26I GTCTC 2 cut(s) 2, 302
AlwI GGATC 4 cut(s) 116, 129, 188, 554
ApeKI GCWGC 2 cut(s) 82, 481
ApoI RAATTY 1 cut(s) 130
Asp700I GAANNNNTTC 1 cut(s) 454
AsuHPI GGTGA 1 cut(s) 38
AsuII TTCGAA 1 cut(s) 370
BamHI GGATCC 1 cut(s) 121
BanII GRGCYC 1 cut(s) 385
Bbv12I GWGCWC 2 cut(s) 148, 385
BbvI GCAGC 2 cut(s) 94, 493
BciT130I CCWGG 3 cut(s) 75, 391, 583
BcoDI GTCTC 2 cut(s) 2, 302
BfaI CTAG 1 cut(s) 180
BisI GCNGC 2 cut(s) 83, 482
BlsI GCNGC 2 cut(s) 84, 483
Bme1390I CCNGG 3 cut(s) 75, 391, 583
BmiI GGNNCC 2 cut(s) 123, 240
BmrFI CCNGG 3 cut(s) 75, 391, 583
BmsI GCATC 1 cut(s) 25
BplI GAGNNNNNCTC 2 cut(s) 253, 285
Bpu14I TTCGAA 1 cut(s) 370
BpuEI CTTGAG 1 cut(s) 121
BsaI GGTCTC 1 cut(s) 302
BsaJI CCNNGG 2 cut(s) 125, 301
Bse1I ACTGG 3 cut(s) 197, 417, 433
BseBI CCWGG 3 cut(s) 75, 391, 583
BseDI CCNNGG 2 cut(s) 125, 301
BseGI GGATG 2 cut(s) 10, 40
BseNI ACTGG 3 cut(s) 197, 417, 433
BseXI GCAGC 2 cut(s) 94, 493
BseYI CCCAGC 1 cut(s) 320
BsiHKAI GWGCWC 2 cut(s) 148, 385
BslFI GGGAC 1 cut(s) 485
BsmAI GTCTC 2 cut(s) 2, 302
BsmFI GGGAC 1 cut(s) 485
BsmI GAATGC 1 cut(s) 438
Bso31I GGTCTC 1 cut(s) 302
Bsp119I TTCGAA 1 cut(s) 370
Bsp1286I GDGCHC 2 cut(s) 148, 385
Bsp143I GATC 3 cut(s) 121, 193, 559
Bsp19I CCATGG 2 cut(s) 125, 301
BspHI TCATGA 1 cut(s) 552
BspLI GGNNCC 2 cut(s) 123, 240
BspPI GGATC 4 cut(s) 116, 129, 188, 554
BspQI GCTCTTC 1 cut(s) 136
BspT104I TTCGAA 1 cut(s) 370
BspTNI GGTCTC 1 cut(s) 302
BsrI ACTGG 3 cut(s) 197, 417, 433
BssECI CCNNGG 2 cut(s) 125, 301
BssMI GATC 3 cut(s) 121, 193, 559
BssNAI GTATAC 1 cut(s) 579
BssT1I CCWWGG 2 cut(s) 125, 301
Bst1107I GTATAC 1 cut(s) 579
Bst2UI CCWGG 3 cut(s) 75, 391, 583
Bst4CI ACNGT 3 cut(s) 50, 187, 534
Bst6I CTCTTC 1 cut(s) 136
BstAPI GCANNNNNTGC 1 cut(s) 152
BstBI TTCGAA 1 cut(s) 370
BstC8I GCNNGC 3 cut(s) 80, 228, 385
BstDSI CCRYGG 2 cut(s) 125, 301
BstF5I GGATG 2 cut(s) 10, 40
BstKTI GATC 3 cut(s) 124, 196, 562
BstMAI GTCTC 2 cut(s) 2, 302
BstMBI GATC 3 cut(s) 121, 193, 559
BstMWI GCNNNNNNNGC 1 cut(s) 152
BstNI CCWGG 3 cut(s) 75, 391, 583
BstNSI RCATGY 1 cut(s) 230
BstSCI CCNGG 3 cut(s) 73, 389, 581
BstV1I GCAGC 2 cut(s) 94, 493
BstX2I RGATCY 3 cut(s) 121, 193, 559
BstYI RGATCY 3 cut(s) 121, 193, 559
BstZ17I GTATAC 1 cut(s) 579
BtgI CCRYGG 2 cut(s) 125, 301
BtsCI GGATG 2 cut(s) 10, 40
BtsIMutI CAGTG 3 cut(s) 258, 410, 426
Cac8I GCNNGC 3 cut(s) 80, 228, 385
CciI TCATGA 1 cut(s) 552
CsiI ACCWGGT 1 cut(s) 581
Csp6I GTAC 1 cut(s) 471
CspCI CAANNNNNGTGG 2 cut(s) 234, 269
CviAII CATG 6 cut(s) 86, 113, 126, 227, 302, 553
CviJI RGCY 6 cut(s) 179, 241, 308, 320, 383, 416
CviKI_1 RGCY 6 cut(s) 179, 241, 308, 320, 383, 416
CviQI GTAC 1 cut(s) 471
DpnI GATC 3 cut(s) 123, 195, 561
DpnII GATC 3 cut(s) 121, 193, 559
Eam1104I CTCTTC 1 cut(s) 136
EarI CTCTTC 1 cut(s) 136
Ecl136II GAGCTC 1 cut(s) 383
Eco130I CCWWGG 2 cut(s) 125, 301
Eco24I GRGCYC 1 cut(s) 385
Eco31I GGTCTC 1 cut(s) 302
Eco53kI GAGCTC 1 cut(s) 383
Eco57I CTGAAG 1 cut(s) 234
EcoICRI GAGCTC 1 cut(s) 383
EcoRII CCWGG 3 cut(s) 73, 389, 581
EcoT14I CCWWGG 2 cut(s) 125, 301
EcoT38I GRGCYC 1 cut(s) 385
ErhI CCWWGG 2 cut(s) 125, 301
FaeI CATG 6 cut(s) 89, 116, 129, 230, 305, 556
FaqI GGGAC 1 cut(s) 485
FatI CATG 6 cut(s) 85, 112, 125, 226, 301, 552
FblI GTMKAC 1 cut(s) 578
Fnu4HI GCNGC 2 cut(s) 83, 482
FokI GGATG 2 cut(s) 17, 47
FriOI GRGCYC 1 cut(s) 385
Fsp4HI GCNGC 2 cut(s) 83, 482
FspBI CTAG 1 cut(s) 180
GluI GCNGC 2 cut(s) 83, 482
GsaI CCCAGC 1 cut(s) 324
Hin1II CATG 6 cut(s) 89, 116, 129, 230, 305, 556
HincII GTYRAC 1 cut(s) 267
HindII GTYRAC 1 cut(s) 267
HinfI GANTC 1 cut(s) 394
HphI GGTGA 1 cut(s) 38
Hpy166II GTNNAC 3 cut(s) 267, 421, 579
Hpy188I TCNGA 2 cut(s) 193, 289
Hpy188III TCNNGA 2 cut(s) 447, 553
Hpy8I GTNNAC 3 cut(s) 267, 421, 579
Hpy99I CGWCG 1 cut(s) 213
HpyAV CCTTC 1 cut(s) 131
HpyCH4III ACNGT 3 cut(s) 50, 187, 534
HpyCH4IV ACGT 1 cut(s) 208
HpyCH4V TGCA 3 cut(s) 116, 155, 436
HpyF10VI GCNNNNNNNGC 1 cut(s) 152
HpySE526I ACGT 1 cut(s) 208
Hsp92II CATG 6 cut(s) 89, 116, 129, 230, 305, 556
Kzo9I GATC 3 cut(s) 121, 193, 559
LguI GCTCTTC 1 cut(s) 136
LmnI GCTCC 3 cut(s) 238, 380, 392
Lsp1109I GCAGC 2 cut(s) 94, 493
LweI GCATC 1 cut(s) 25
MabI ACCWGGT 1 cut(s) 581
MaeI CTAG 1 cut(s) 180
MaeII ACGT 1 cut(s) 208
MaeIII GTNAC 2 cut(s) 95, 204
MalI GATC 3 cut(s) 123, 195, 561
MboI GATC 3 cut(s) 121, 193, 559
MboII GAAGA 1 cut(s) 153
MflI RGATCY 3 cut(s) 121, 193, 559
MhlI GDGCHC 2 cut(s) 148, 385
MluCI AATT 3 cut(s) 29, 130, 450
MmeI TCCRAC 1 cut(s) 269
MnlI CCTC 6 cut(s) 49, 252, 255, 458, 508, 560
MroXI GAANNNNTTC 1 cut(s) 454
MslI CAYNNNNRTG 3 cut(s) 90, 225, 431
MspR9I CCNGG 3 cut(s) 75, 391, 583
Mva1269I GAATGC 1 cut(s) 438
MvaI CCWGG 3 cut(s) 75, 391, 583
MwoI GCNNNNNNNGC 1 cut(s) 152
NcoI CCATGG 2 cut(s) 125, 301
NdeII GATC 3 cut(s) 121, 193, 559
NlaIII CATG 6 cut(s) 89, 116, 129, 230, 305, 556
NlaIV GGNNCC 2 cut(s) 123, 240
NmuCI GTSAC 1 cut(s) 95
NspI RCATGY 1 cut(s) 230
NspV TTCGAA 1 cut(s) 370
PaeI GCATGC 1 cut(s) 230
PagI TCATGA 1 cut(s) 552
PciSI GCTCTTC 1 cut(s) 136
PctI GAATGC 1 cut(s) 438
PdmI GAANNNNTTC 1 cut(s) 454
PfeI GAWTC 1 cut(s) 394
PfoI TCCNGGA 1 cut(s) 389
PkrI GCNGC 2 cut(s) 84, 483
PsiI TTATAA 1 cut(s) 338
Psp124BI GAGCTC 1 cut(s) 385
Psp6I CCWGG 3 cut(s) 73, 389, 581
PspFI CCCAGC 1 cut(s) 320
PspGI CCWGG 3 cut(s) 73, 389, 581
PspN4I GGNNCC 2 cut(s) 123, 240
PsuI RGATCY 3 cut(s) 121, 193, 559
RsaI GTAC 1 cut(s) 472
RsaNI GTAC 1 cut(s) 471
RseI CAYNNNNRTG 3 cut(s) 90, 225, 431
SacI GAGCTC 1 cut(s) 385
SapI GCTCTTC 1 cut(s) 136
SatI GCNGC 2 cut(s) 83, 482
Sau3AI GATC 3 cut(s) 121, 193, 559
ScrFI CCNGG 3 cut(s) 75, 391, 583
SduI GDGCHC 2 cut(s) 148, 385
SetI ASST 8 cut(s) 76, 167, 211, 266, 385, 469, 552, 587
SexAI ACCWGGT 1 cut(s) 581
SfaNI GCATC 1 cut(s) 25
SfuI TTCGAA 1 cut(s) 370
SmiMI CAYNNNNRTG 3 cut(s) 90, 225, 431
SmlI CTYRAG 1 cut(s) 100
SmoI CTYRAG 1 cut(s) 100
SphI GCATGC 1 cut(s) 230
Sse9I AATT 3 cut(s) 29, 130, 450
SspMI CTAG 1 cut(s) 180
SstI GAGCTC 1 cut(s) 385
StyD4I CCNGG 3 cut(s) 73, 389, 581
StyI CCWWGG 2 cut(s) 125, 301
TaaI ACNGT 3 cut(s) 50, 187, 534
TaiI ACGT 1 cut(s) 211
TaqI TCGA 2 cut(s) 370, 448
TasI AATT 3 cut(s) 29, 130, 450
TfiI GAWTC 1 cut(s) 394
TscAI CASTG 3 cut(s) 258, 417, 433
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 2 cut(s) 82, 481
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 4 cut(s) 36, 83, 447, 479
TspRI CASTG 3 cut(s) 258, 417, 433
XapI RAATTY 1 cut(s) 130
XceI RCATGY 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 578
XmnI GAANNNNTTC 1 cut(s) 454
XspI CTAG 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.