Rorug07G0288700

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001294
Physical Location & Seq
Reverse (-)
28049221 .. 28054277
5057 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0288700.1

Sequence Viewer

Length: 2217 bp
ATGAAGGGAGCTGCGCTTGTGGCTTTTGCTGCTACAATTGGTAACTTTCTGCAAGGATGGGACAATGCTACAATTGCTGGGGCTATTGTTTACATCACGGACGATTTTGATTTAAGTAGCTCGGTAGAAGGTCTTGTTGTGGCCATGTCACTCATCGGGGCAACAGTTATTACAACATGCTCAGGAGGCATATCAGATTGGCTTGGTCGGAGGCCAATGCTAATAGCATCATCAGTTCTTTATTTTGTGAGTGGCTTGGTGATGTTGTGGTCACCCAATGTGTATGTACTATGTCTTGCAAGGCTATTAGATGGATTTGGAATTGGTTTAGCGGTTACTCTTGTCCCAGTCTACATATCCGAGACCGCCCCATCAGATATAAGGGGATCGTTGAATACTCTTCCACAGTTCCTTGGTTCAGGAGGCATGTTTTTGTCATATTGTATGGTTTTCGGGATGTCGCTGTTGGCCTCGCCAAGCTGGAGACTGATGCTTGGGATTCTTTCCATTCTCTCTCTTATATATTTTGTATTAACCGTGTTTTACTTACCTGAATCTCCTCGATGGCTTGTGAGTAAGGGCAGGATGCTCGAGGCAAAGAAGGTTCTCCAGAGCTTGCGTGGCACTGAAGATGTTTCTGGTGAGATGGCTTTGCTTGTCGAAGGTCTTGGAGTTGGAGGTGAACCATCTTTAGAAGAGTACATCATAGGCCCAGCTGATGATCTTGATGGTCAGGAAACAGCTGACAAAGACAAAATCAGGTTATATGGACCCGAAGAGGGCCTTTCCTGGGTTGCCAGACCTGTAACTGGGCAGGGTTCTATTGTCAGTCTTGTCTCTCGCCAAGGAAGCATGGCAACCCAGAATGTGCCTCTAATGGATCCTCTTGTCACTCTCTTTGGTAGTGTCCATGAAAACTTCCCAGAGGCAGGGAGTACGCGGGGAAGCATGCTCTTTTCTAACTTTGGCAGCATGTTCAGCACAGCAGATCATCCACGGGGTAAAACTGAACAATGGGATGAAGAGAGCTTGCATAGGGAAGGTGAGGACTATGCATCTGGAGGAGACTCAGATGACAATGTGCATAGTCCTTTGATTTCATGCCAGACGACAAGTATGGAAAAGGATATGGTGCCACCTCCTCCTTCCCATGGTAGTATTCTAAGCATGAGGCGCCACAGCAGTCTCATGCAAGGAACTGGGGAGACAGTTGGTAGCACAGGCATTGGTGGTGGGTGGCAGTTGGCATGGAAGTGGTCTGAGAGACAAGGCGAGGATGGAAAGAAGGAAGGAGGATTCCAGAGGGTATATTTGCACCAGGAAGGAGTCCCTGGCTCACGTCCTGGGTCCCTTGTGTCACTTCCTGGTGGTGATGTTCCTGCAGAAGGCGAGTTCATCCAGGCAGCCGCTCTGGTCAGTCAGCCTGCTTTGTATTCGAAGTCACTTATCGATCAGCATCCTGTTGGACCTGCCATGGTTCATCCATCAGAAACAGCTTCGAAAGGACCAATATGGGCTGCTCTGCTTGAACCAGGGATTAAGCACGCATTGTTTGTTGGAATTGGAATCCAGATTCTTCAGCAGTTTTCTGGGATAAATGGAGTTCTCTATTACACTCCTCAAATTCTTGAAGAGGCTGGAGTCTCAGTTCTTCTTGAAAGCTTCGGTCTCAGTACAACATCTGCATCTTTCCTCATCAGTGCATTCACAACTTTGTTGATGCTTCCTTGTATAGGTGTAGGCATGAAGCTCATGGATATCACTGGTAGAAGGATCCTGCTACTGACTACAGTTCCTGTGTTGATTGTGTCACTCATTCTTCTAGTCATCGCCAACTTAGTGACCCTCGGTACAGTCGTTGAAGCCGCAATATCAACCACTTGTGTGATAATCTATTTCTGCGTCTTTGTCACGGCCTATGGGCCAATCCCAAATATCCTCTGCTCCGAGATCTTTCCGACGAGGGTGCGTGGCCTCTGCATTGCCATCTGTGCCCTGGCGTACTGGATTTCAGACATTATCATCACCTACTCGCTGCCAGTTCTGCTTGATTCAATAGGCCTAGCTGGTATCTTTGGGATTTATGCTGTTGTTTGTGTCATCTCTTTGGTGTTTATCTACTTGAAAGTTCCAGAAACCAAAGGCATGCCCCTTGAAGTCATTACTGAATTCTTTTCTGTTGGTGCAAGACAAATTGCTGCTGCCAAAAATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

738

Amino Acids

78.78

Weight (kDa)

4.87

Isoelectric Point (pI)

37.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sugar_tr PF00083 7 - 221 2.3e-49 Sugar (and other) transporter
MFS_1 PF07690 12 - 194 2.2e-23 Major Facilitator Superfamily
Sugar_tr PF00083 505 - 725 1.4e-38 Sugar (and other) transporter
MFS_1 PF07690 550 - 714 2e-07 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1477
AccB1I GGYRCC 2 cut(s) 1132, 1173
AccBSI CCGCTC 1 cut(s) 1409
AccI GTMKAC 1 cut(s) 351
AccII CGCG 1 cut(s) 940
AciI CCGC 5 cut(s) 332, 366, 940, 1407, 1866
AclWI GGATC 5 cut(s) 394, 875, 888, 1768, 1781
AcoI YGGCCR 1 cut(s) 141
AcsI RAATTY 2 cut(s) 1623, 2168
AcuI CTGAAG 2 cut(s) 648, 1562
AcyI GRCGYC 1 cut(s) 1174
AfaI GTAC 6 cut(s) 288, 701, 937, 1675, 1852, 2003
AfiI CCNNNNNNNGG 7 cut(s) 779, 790, 809, 929, 1151, 1385, 1733
AgsI TTSAA 8 cut(s) 394, 1529, 1631, 1658, 1862, 2055, 2125, 2156
AjiI CACGTC 1 cut(s) 1340
AjnI CCWGG 8 cut(s) 788, 1317, 1330, 1342, 1363, 1398, 1531, 1995
AleI CACNNNNGTG 1 cut(s) 1883
AloI GAACNNNNNNTCC 2 cut(s) 1632, 1664
Alw26I GTCTC 9 cut(s) 356, 478, 841, 1059, 1190, 1199, 1258, 1648, 1673
AlwI GGATC 5 cut(s) 394, 875, 888, 1768, 1781
AlwNI CAGNNNCTG 1 cut(s) 1796
Ama87I CYCGRG 1 cut(s) 590
AoxI GGCC 9 cut(s) 141, 212, 468, 709, 781, 1914, 1922, 1972, 2059
ApeKI GCWGC 8 cut(s) 11, 29, 969, 1403, 1517, 2035, 2198, 2201
ApoI RAATTY 2 cut(s) 1623, 2168
AspLEI GCGC 2 cut(s) 16, 1176
AspS9I GGNCC 7 cut(s) 710, 770, 781, 1347, 1466, 1505, 1922
AsuHPI GGTGA 7 cut(s) 264, 271, 653, 692, 1055, 1382, 2017
AsuII TTCGAA 2 cut(s) 1436, 1499
AvaI CYCGRG 1 cut(s) 590
AvaII GGWCC 4 cut(s) 770, 1347, 1466, 1505
BaeGI GKGCMC 1 cut(s) 1996
BaeI ACNNNNGTAYC 2 cut(s) 1842, 1875
BalI TGGCCA 1 cut(s) 143
BamHI GGATCC 2 cut(s) 880, 1773
BanI GGYRCC 2 cut(s) 1132, 1173
BbvI GCAGC 7 cut(s) 16, 981, 1415, 1504, 2022, 2185, 2188
BceAI ACGGC 1 cut(s) 1929
BcgI CGANNNNNNTGC 4 cut(s) 571, 605, 1948, 1982
BciT130I CCWGG 8 cut(s) 790, 1319, 1332, 1344, 1365, 1400, 1533, 1997
BcoDI GTCTC 9 cut(s) 356, 478, 841, 1059, 1190, 1199, 1258, 1648, 1673
BfaI CTAG 2 cut(s) 1823, 2063
BfmI CTRYAG 2 cut(s) 1380, 1788
BfoI RGCGCY 1 cut(s) 1177
BfuAI ACCTGC 1 cut(s) 1477
BglII AGATCT 1 cut(s) 1950
Bme1390I CCNGG 8 cut(s) 790, 1319, 1332, 1344, 1365, 1400, 1533, 1997
Bme18I GGWCC 4 cut(s) 770, 1347, 1466, 1505
BmeT110I CYCGRG 1 cut(s) 590
BmgBI CACGTC 1 cut(s) 1340
BmgT120I GGNCC 7 cut(s) 710, 770, 781, 1347, 1466, 1505, 1922
BmiI GGNNCC 7 cut(s) 772, 882, 1134, 1175, 1348, 1349, 1775
BmrFI CCNGG 8 cut(s) 790, 1319, 1332, 1344, 1365, 1400, 1533, 1997
BmrI ACTGGG 3 cut(s) 341, 819, 1209
BmsI GCATC 7 cut(s) 236, 480, 576, 1064, 1465, 1694, 1710
BmuI ACTGGG 3 cut(s) 341, 819, 1209
BpmI CTGGAG 4 cut(s) 502, 593, 1080, 1659
Bpu10I CCTNAGC 1 cut(s) 181
Bpu14I TTCGAA 2 cut(s) 1436, 1499
Bsa29I ATCGAT 1 cut(s) 1449
BsaBI GATNNNNATC 1 cut(s) 1455
BsaHI GRCGYC 1 cut(s) 1174
BsaI GGTCTC 2 cut(s) 356, 1673
BsaXI ACNNNNNCTCC 2 cut(s) 1632, 1662
Bsc4I CCNNNNNNNGG 7 cut(s) 779, 790, 809, 929, 1151, 1385, 1733
Bse1I ACTGG 6 cut(s) 347, 814, 1204, 1768, 2009, 2039
Bse3DI GCAATG 1 cut(s) 1980
Bse8I GATNNNNATC 1 cut(s) 1455
BseBI CCWGG 8 cut(s) 790, 1319, 1332, 1344, 1365, 1400, 1533, 1997
BseCI ATCGAT 1 cut(s) 1449
BseGI GGATG 9 cut(s) 62, 462, 591, 991, 1024, 1282, 1395, 1456, 1480
BseJI GATNNNNATC 1 cut(s) 1455
BseLI CCNNNNNNNGG 7 cut(s) 779, 790, 809, 929, 1151, 1385, 1733
BseMI GCAATG 1 cut(s) 1980
BseMII CTCAG 5 cut(s) 195, 1083, 1251, 1659, 1684
BseNI ACTGG 6 cut(s) 347, 814, 1204, 1768, 2009, 2039
BseRI GAGGAG 4 cut(s) 549, 1077, 1131, 1608
BseSI GKGCMC 1 cut(s) 1996
BseXI GCAGC 7 cut(s) 16, 981, 1415, 1504, 2022, 2185, 2188
BseYI CCCAGC 2 cut(s) 77, 712
Bsh1236I CGCG 1 cut(s) 940
BshFI GGCC 9 cut(s) 143, 214, 470, 711, 783, 1916, 1924, 1974, 2061
BshNI GGYRCC 2 cut(s) 1132, 1173
BshVI ATCGAT 1 cut(s) 1449
BsiHKCI CYCGRG 1 cut(s) 590
BslFI GGGAC 4 cut(s) 74, 329, 1313, 1333
BslI CCNNNNNNNGG 7 cut(s) 779, 790, 809, 929, 1151, 1385, 1733
BsmAI GTCTC 9 cut(s) 356, 478, 841, 1059, 1190, 1199, 1258, 1648, 1673
BsmFI GGGAC 4 cut(s) 74, 329, 1313, 1333
BsmI GAATGC 1 cut(s) 1703
BsnI GGCC 9 cut(s) 143, 214, 470, 711, 783, 1916, 1924, 1974, 2061
Bso31I GGTCTC 2 cut(s) 356, 1673
BsoBI CYCGRG 1 cut(s) 590
Bsp119I TTCGAA 2 cut(s) 1436, 1499
Bsp1286I GDGCHC 1 cut(s) 1996
Bsp143I GATC 7 cut(s) 386, 721, 880, 988, 1450, 1773, 1950
Bsp19I CCATGG 2 cut(s) 1150, 1473
BspACI CCGC 5 cut(s) 332, 366, 940, 1407, 1866
BspANI GGCC 9 cut(s) 143, 214, 470, 711, 783, 1916, 1924, 1974, 2061
BspCNI CTCAG 5 cut(s) 194, 1082, 1252, 1658, 1683
BspDI ATCGAT 1 cut(s) 1449
BspFNI CGCG 1 cut(s) 940
BspLI GGNNCC 7 cut(s) 772, 882, 1134, 1175, 1348, 1349, 1775
BspMAI CTGCAG 1 cut(s) 1384
BspMI ACCTGC 1 cut(s) 1477
BspPI GGATC 5 cut(s) 394, 875, 888, 1768, 1781
BspT104I TTCGAA 2 cut(s) 1436, 1499
BspT107I GGYRCC 2 cut(s) 1132, 1173
BspTNI GGTCTC 2 cut(s) 356, 1673
BsrBI CCGCTC 1 cut(s) 1409
BsrDI GCAATG 1 cut(s) 1980
BsrI ACTGG 6 cut(s) 347, 814, 1204, 1768, 2009, 2039
BssMI GATC 7 cut(s) 386, 721, 880, 988, 1450, 1773, 1950
BssNI GRCGYC 1 cut(s) 1174
BssT1I CCWWGG 4 cut(s) 412, 844, 1150, 1473
Bst2UI CCWGG 8 cut(s) 790, 1319, 1332, 1344, 1365, 1400, 1533, 1997
Bst4CI ACNGT 6 cut(s) 166, 408, 538, 1210, 1792, 1855
Bst6I CTCTTC 5 cut(s) 405, 690, 771, 1017, 1626
BstACI GRCGYC 1 cut(s) 1174
BstBI TTCGAA 2 cut(s) 1436, 1499
BstC8I GCNNGC 6 cut(s) 617, 950, 1031, 1425, 1545, 2147
BstDEI CTNAG 7 cut(s) 181, 1069, 1163, 1260, 1645, 1670, 1837
BstDSI CCRYGG 3 cut(s) 995, 1150, 1473
BstEII GGTNACC 1 cut(s) 270
BstENI CCTNNNNNAGG 2 cut(s) 1383, 1731
BstF5I GGATG 9 cut(s) 62, 462, 591, 991, 1024, 1282, 1395, 1456, 1480
BstFNI CGCG 1 cut(s) 940
BstH2I RGCGCY 1 cut(s) 1177
BstHHI GCGC 2 cut(s) 16, 1176
BstKTI GATC 7 cut(s) 389, 724, 883, 991, 1453, 1776, 1953
BstMAI GTCTC 9 cut(s) 356, 478, 841, 1059, 1190, 1199, 1258, 1648, 1673
BstMBI GATC 7 cut(s) 386, 721, 880, 988, 1450, 1773, 1950
BstNI CCWGG 8 cut(s) 790, 1319, 1332, 1344, 1365, 1400, 1533, 1997
BstNSI RCATGY 5 cut(s) 180, 430, 952, 976, 2149
BstPI GGTNACC 1 cut(s) 270
BstSCI CCNGG 8 cut(s) 788, 1317, 1330, 1342, 1363, 1398, 1531, 1995
BstSFI CTRYAG 2 cut(s) 1380, 1788
BstSLI GKGCMC 1 cut(s) 1996
BstUI CGCG 1 cut(s) 940
BstV1I GCAGC 7 cut(s) 16, 981, 1415, 1504, 2022, 2185, 2188
BstX2I RGATCY 3 cut(s) 880, 1773, 1950
BstYI RGATCY 3 cut(s) 880, 1773, 1950
Bsu15I ATCGAT 1 cut(s) 1449
BsuRI GGCC 9 cut(s) 143, 214, 470, 711, 783, 1916, 1924, 1974, 2061
BsuTUI ATCGAT 1 cut(s) 1449
BtgI CCRYGG 3 cut(s) 995, 1150, 1473
BtgZI GCGATG 1 cut(s) 1813
BtrI CACGTC 1 cut(s) 1340
BtsCI GGATG 9 cut(s) 62, 462, 591, 991, 1024, 1282, 1395, 1456, 1480
BtsIMutI CAGTG 3 cut(s) 624, 1705, 1761
BveI ACCTGC 1 cut(s) 1477
Cac8I GCNNGC 6 cut(s) 617, 950, 1031, 1425, 1545, 2147
CaiI CAGNNNCTG 1 cut(s) 1796
CfoI GCGC 2 cut(s) 16, 1176
Cfr13I GGNCC 7 cut(s) 710, 770, 781, 1347, 1466, 1505, 1922
ClaI ATCGAT 1 cut(s) 1449
CseI GACGC 1 cut(s) 1891
Csp6I GTAC 6 cut(s) 287, 700, 936, 1674, 1851, 2002
CviQI GTAC 6 cut(s) 287, 700, 936, 1674, 1851, 2002
DdeI CTNAG 7 cut(s) 181, 1069, 1163, 1260, 1645, 1670, 1837
DinI GGCGCC 1 cut(s) 1175
DpnI GATC 7 cut(s) 388, 723, 882, 990, 1452, 1775, 1952
DpnII GATC 7 cut(s) 386, 721, 880, 988, 1450, 1773, 1950
EaeI YGGCCR 1 cut(s) 141
Eam1104I CTCTTC 5 cut(s) 405, 690, 771, 1017, 1626
EarI CTCTTC 5 cut(s) 405, 690, 771, 1017, 1626
Eco130I CCWWGG 4 cut(s) 412, 844, 1150, 1473
Eco147I AGGCCT 1 cut(s) 2061
Eco31I GGTCTC 2 cut(s) 356, 1673
Eco32I GATATC 1 cut(s) 1759
Eco47I GGWCC 4 cut(s) 770, 1347, 1466, 1505
Eco57I CTGAAG 2 cut(s) 648, 1562
Eco88I CYCGRG 1 cut(s) 590
Eco91I GGTNACC 1 cut(s) 270
EcoNI CCTNNNNNAGG 2 cut(s) 1383, 1731
EcoO109I RGGNCCY 2 cut(s) 781, 1347
EcoO65I GGTNACC 1 cut(s) 270
EcoRI GAATTC 1 cut(s) 2168
EcoRII CCWGG 8 cut(s) 788, 1317, 1330, 1342, 1363, 1398, 1531, 1995
EcoRV GATATC 1 cut(s) 1759
EcoT14I CCWWGG 4 cut(s) 412, 844, 1150, 1473
EcoT22I ATGCAT 1 cut(s) 1057
EgeI GGCGCC 1 cut(s) 1175
EheI GGCGCC 1 cut(s) 1175
ErhI CCWWGG 4 cut(s) 412, 844, 1150, 1473
FalI AAGNNNNNCTT 2 cut(s) 768, 800
FaqI GGGAC 4 cut(s) 74, 329, 1313, 1333
FauI CCCGC 1 cut(s) 933
FblI GTMKAC 1 cut(s) 351
FokI GGATG 9 cut(s) 69, 469, 598, 978, 1031, 1289, 1382, 1443, 1467
FspBI CTAG 2 cut(s) 1823, 2063
GlaI GCGC 2 cut(s) 15, 1175
GsaI CCCAGC 2 cut(s) 81, 716
GsuI CTGGAG 4 cut(s) 502, 593, 1080, 1659
HaeII RGCGCY 1 cut(s) 1177
HaeIII GGCC 9 cut(s) 143, 214, 470, 711, 783, 1916, 1924, 1974, 2061
HgaI GACGC 1 cut(s) 1891
HhaI GCGC 2 cut(s) 16, 1176
Hin1I GRCGYC 1 cut(s) 1174
Hin6I GCGC 2 cut(s) 14, 1174
HinP1I GCGC 2 cut(s) 14, 1174
HindIII AAGCTT 1 cut(s) 1660
HinfI GANTC 9 cut(s) 499, 554, 1067, 1296, 1326, 1566, 1573, 1641, 2051
HphI GGTGA 7 cut(s) 264, 271, 653, 692, 1055, 1382, 2017
Hpy166II GTNNAC 3 cut(s) 91, 352, 683
Hpy8I GTNNAC 3 cut(s) 91, 352, 683
Hpy99I CGWCG 1 cut(s) 1963
HpyCH4III ACNGT 6 cut(s) 166, 408, 538, 1210, 1792, 1855
HpyCH4IV ACGT 1 cut(s) 1339
HpyF3I CTNAG 7 cut(s) 181, 1069, 1163, 1260, 1645, 1670, 1837
HpySE526I ACGT 1 cut(s) 1339
Hsp92I GRCGYC 1 cut(s) 1174
HspAI GCGC 2 cut(s) 14, 1174
KasI GGCGCC 1 cut(s) 1173
KflI GGGWCCC 1 cut(s) 1347
Kzo9I GATC 7 cut(s) 386, 721, 880, 988, 1450, 1773, 1950
LmnI GCTCC 2 cut(s) 8, 1949
Lsp1109I GCAGC 7 cut(s) 16, 981, 1415, 1504, 2022, 2185, 2188
LweI GCATC 7 cut(s) 236, 480, 576, 1064, 1465, 1694, 1710
MaeI CTAG 2 cut(s) 1823, 2063
MaeII ACGT 1 cut(s) 1339
MalI GATC 7 cut(s) 388, 723, 882, 990, 1452, 1775, 1952
MbiI CCGCTC 1 cut(s) 1409
MboI GATC 7 cut(s) 386, 721, 880, 988, 1450, 1773, 1950
MboII GAAGA 9 cut(s) 392, 641, 707, 788, 1034, 1568, 1643, 1643, 1811
MfeI CAATTG 2 cut(s) 36, 72
MflI RGATCY 3 cut(s) 880, 1773, 1950
MhlI GDGCHC 1 cut(s) 1996
MlsI TGGCCA 1 cut(s) 143
MluCI AATT 7 cut(s) 36, 72, 321, 1560, 1623, 2168, 2193
MluNI TGGCCA 1 cut(s) 143
Mly113I GGCGCC 1 cut(s) 1174
MlyI GAGTC 3 cut(s) 1061, 1335, 1650
MmeI TCCRAC 5 cut(s) 188, 655, 1444, 1537, 1982
Mox20I TGGCCA 1 cut(s) 143
Mph1103I ATGCAT 1 cut(s) 1057
MscI TGGCCA 1 cut(s) 143
MseI TTAA 3 cut(s) 113, 533, 1539
MslI CAYNNNNRTG 2 cut(s) 1252, 1883
Msp20I TGGCCA 1 cut(s) 143
MspA1I CMGCKG 2 cut(s) 716, 743
MspR9I CCNGG 8 cut(s) 790, 1319, 1332, 1344, 1365, 1400, 1533, 1997
MunI CAATTG 2 cut(s) 36, 72
Mva1269I GAATGC 1 cut(s) 1703
MvaI CCWGG 8 cut(s) 790, 1319, 1332, 1344, 1365, 1400, 1533, 1997
MvnI CGCG 1 cut(s) 940
NarI GGCGCC 1 cut(s) 1174
NcoI CCATGG 2 cut(s) 1150, 1473
NdeII GATC 7 cut(s) 386, 721, 880, 988, 1450, 1773, 1950
NlaIV GGNNCC 7 cut(s) 772, 882, 1134, 1175, 1348, 1349, 1775
NmuCI GTSAC 8 cut(s) 147, 270, 889, 1356, 1440, 1809, 1840, 1909
NsiI ATGCAT 1 cut(s) 1057
NspI RCATGY 5 cut(s) 180, 430, 952, 976, 2149
NspV TTCGAA 2 cut(s) 1436, 1499
OliI CACNNNNGTG 1 cut(s) 1883
PaeI GCATGC 2 cut(s) 952, 2149
PaeR7I CTCGAG 1 cut(s) 590
PceI AGGCCT 1 cut(s) 2061
PcsI WCGNNNNNNNCGW 1 cut(s) 1854
PctI GAATGC 1 cut(s) 1703
PfeI GAWTC 6 cut(s) 499, 554, 1296, 1566, 1573, 2051
PleI GAGTC 3 cut(s) 1061, 1334, 1649
PluTI GGCGCC 1 cut(s) 1177
PpsI GAGTC 3 cut(s) 1061, 1334, 1649
PpuMI RGGWCCY 1 cut(s) 1347
Psp5II RGGWCCY 1 cut(s) 1347
Psp6I CCWGG 8 cut(s) 788, 1317, 1330, 1342, 1363, 1398, 1531, 1995
PspEI GGTNACC 1 cut(s) 270
PspFI CCCAGC 2 cut(s) 77, 712
PspGI CCWGG 8 cut(s) 788, 1317, 1330, 1342, 1363, 1398, 1531, 1995
PspN4I GGNNCC 7 cut(s) 772, 882, 1134, 1175, 1348, 1349, 1775
PspPI GGNCC 7 cut(s) 710, 770, 781, 1347, 1466, 1505, 1922
PspPPI RGGWCCY 1 cut(s) 1347
PspXI VCTCGAGB 1 cut(s) 590
PstI CTGCAG 1 cut(s) 1384
PstNI CAGNNNCTG 1 cut(s) 1796
PsuI RGATCY 3 cut(s) 880, 1773, 1950
PvuII CAGCTG 2 cut(s) 716, 743
RsaI GTAC 6 cut(s) 288, 701, 937, 1675, 1852, 2003
RsaNI GTAC 6 cut(s) 287, 700, 936, 1674, 1851, 2002
RseI CAYNNNNRTG 2 cut(s) 1252, 1883
SaqAI TTAA 3 cut(s) 113, 533, 1539
Sau3AI GATC 7 cut(s) 386, 721, 880, 988, 1450, 1773, 1950
Sau96I GGNCC 7 cut(s) 710, 770, 781, 1347, 1466, 1505, 1922
SchI GAGTC 3 cut(s) 1061, 1335, 1650
ScrFI CCNGG 8 cut(s) 790, 1319, 1332, 1344, 1365, 1400, 1533, 1997
SduI GDGCHC 1 cut(s) 1996
SfaNI GCATC 7 cut(s) 236, 480, 576, 1064, 1465, 1694, 1710
SfcI CTRYAG 2 cut(s) 1380, 1788
SfoI GGCGCC 1 cut(s) 1175
Sfr274I CTCGAG 1 cut(s) 590
SfuI TTCGAA 2 cut(s) 1436, 1499
SinI GGWCC 4 cut(s) 770, 1347, 1466, 1505
SlaI CTCGAG 1 cut(s) 590
SmiMI CAYNNNNRTG 2 cut(s) 1252, 1883
SmlI CTYRAG 1 cut(s) 590
SmoI CTYRAG 1 cut(s) 590
SphI GCATGC 2 cut(s) 952, 2149
Sse9I AATT 7 cut(s) 36, 72, 321, 1560, 1623, 2168, 2193
SseBI AGGCCT 1 cut(s) 2061
SsiI CCGC 5 cut(s) 332, 366, 940, 1407, 1866
SspDI GGCGCC 1 cut(s) 1173
SspMI CTAG 2 cut(s) 1823, 2063
StuI AGGCCT 1 cut(s) 2061
StyD4I CCNGG 8 cut(s) 788, 1317, 1330, 1342, 1363, 1398, 1531, 1995
StyI CCWWGG 4 cut(s) 412, 844, 1150, 1473
TaaI ACNGT 6 cut(s) 166, 408, 538, 1210, 1792, 1855
TaiI ACGT 1 cut(s) 1342
TaqI TCGA 6 cut(s) 562, 591, 660, 1436, 1449, 1499
TaqII GACCGA 1 cut(s) 1655
TasI AATT 7 cut(s) 36, 72, 321, 1560, 1623, 2168, 2193
TatI WGTACW 3 cut(s) 286, 699, 1673
TauI GCSGC 2 cut(s) 1409, 1868
TfiI GAWTC 6 cut(s) 499, 554, 1296, 1566, 1573, 2051
Tru1I TTAA 3 cut(s) 113, 533, 1539
Tru9I TTAA 3 cut(s) 113, 533, 1539
TscAI CASTG 3 cut(s) 631, 1705, 1768
TseFI GTSAC 8 cut(s) 147, 270, 889, 1356, 1440, 1809, 1840, 1909
TseI GCWGC 8 cut(s) 11, 29, 969, 1403, 1517, 2035, 2198, 2201
Tsp45I GTSAC 8 cut(s) 147, 270, 889, 1356, 1440, 1809, 1840, 1909
TspDTI ATGAA 7 cut(s) 17, 927, 1035, 1089, 1384, 1469, 1760
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 3 cut(s) 631, 1705, 1768
VpaK11BI GGWCC 4 cut(s) 770, 1347, 1466, 1505
XagI CCTNNNNNAGG 2 cut(s) 1383, 1731
XapI RAATTY 2 cut(s) 1623, 2168
XceI RCATGY 5 cut(s) 180, 430, 952, 976, 2149
XcmI CCANNNNNNNNNTGG 2 cut(s) 617, 1993
XhoI CTCGAG 1 cut(s) 590
XmiI GTMKAC 1 cut(s) 351
XspI CTAG 2 cut(s) 1823, 2063
Zsp2I ATGCAT 1 cut(s) 1057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.