RLG00000019308

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001294
Physical Location & Seq
Reverse (-)
50085802 .. 50086668
867 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019308

Sequence Viewer

Length: 867 bp
ATGAAGAGGAAGGCGGAAGTGAGGTTCGGTTCAGGTCATAAGTGGAAGAGAGGAAAAAAGATCGGCGAAGGAGGCTTCGGTTCTGTGTTCGTTGCATTTTCCAAGAGGCCCTATATCATCACCGATCATATGCCGAAGGCCATGGCTGTGAAATCAGCAAAGATCAAGGATTCCGATTCCATTCGATACGAGCTGGAGGTGTTTAGGGAGTTGAATAACGAGGGCGGTTCTTGCCGCTTCATCATTGAGCATTATGGTGAGGAGGTTACCTGGAGTGATGAGGGCGAAGAGGTCTATAACTTGGCCTTGGAAGTAGCATGGGGAAGCCTTGCTAGGTTGATAAAGAATATCGGTCATGGGTTGCAGGAATCCGATGTAAGGCGGTATACCGGTGATATTCTTGAAGGTATTAAACACATACACAAGAGTGGGTATGTCCACTGTGATTTGAAACCAGGTAACATCTTGCTTGTGGAAAAGAAAGGTGTTCATCGCTATAGCTTGGTGGCCAAAGTTGGTGACTTGGGGCTGGCCAAGAAGGAGGAGGTTCAGGAACGCTGGAGAGGCACACCCATGTACTTGGCTCCTGAAACTGTCGACAACAATGAGCAAGAACAACCCAGTGACATATGGGCTGTGGGGTGTATTGTTCTGGAAATGCTTACGGGAGAGTGCCCCCGGAATCATTTCTATGACGGGGAGAGTGATACTCTCCACATTGTTGATGGAAAGGAGCCAAAAATTCCTGATACGATCTCGAGTCTTGCTAAGGAGTTTCTAGAGCTTTGCCTGGCTGAGTTTCCTGAGCAAAGGTGGACAGCTGGAGAACTTCTAACTCATCCCTTCGTGGCAGATGTTGATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

289

Amino Acids

32.58

Weight (kDa)

5.82

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 16 - 283 5.9e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 17 - 278 2e-24 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 386, 597
AciI CCGC 4 cut(s) 14, 225, 235, 382
AcoI YGGCCR 2 cut(s) 507, 531
AcsI RAATTY 1 cut(s) 741
AfaI GTAC 1 cut(s) 578
AfiI CCNNNNNNNGG 1 cut(s) 378
AgeI ACCGGT 1 cut(s) 389
AgsI TTSAA 3 cut(s) 214, 404, 451
AjnI CCWGG 3 cut(s) 269, 454, 789
AleI CACNNNNGTG 1 cut(s) 426
AloI GAACNNNNNNTCC 2 cut(s) 8, 40
AluBI AGCT 4 cut(s) 193, 501, 784, 821
AluI AGCT 4 cut(s) 193, 501, 784, 821
Ama87I CYCGRG 1 cut(s) 757
AoxI GGCC 5 cut(s) 107, 138, 303, 507, 531
ApoI RAATTY 1 cut(s) 741
AsiGI ACCGGT 1 cut(s) 389
Asp700I GAANNNNTTC 1 cut(s) 686
AspS9I GGNCC 1 cut(s) 108
AsuC2I CCSGG 1 cut(s) 679
AsuHPI GGTGA 4 cut(s) 112, 269, 404, 530
AvaI CYCGRG 1 cut(s) 757
BaeGI GKGCMC 1 cut(s) 677
BalI TGGCCA 2 cut(s) 509, 533
BccI CCATC 1 cut(s) 719
BciT130I CCWGG 3 cut(s) 271, 456, 791
BcnI CCSGG 1 cut(s) 679
BfaI CTAG 2 cut(s) 333, 779
BfmI CTRYAG 1 cut(s) 496
BisI GCNGC 1 cut(s) 235
BlsI GCNGC 1 cut(s) 236
Bme1390I CCNGG 4 cut(s) 271, 456, 679, 791
BmeT110I CYCGRG 1 cut(s) 757
BmgT120I GGNCC 1 cut(s) 108
BmiI GGNNCC 2 cut(s) 585, 735
BmrFI CCNGG 4 cut(s) 271, 456, 679, 791
BmrI ACTGGG 1 cut(s) 615
BmuI ACTGGG 1 cut(s) 615
BplI GAGNNNNNCTC 2 cut(s) 694, 726
BpmI CTGGAG 4 cut(s) 215, 292, 580, 843
Bpu10I CCTNAGC 2 cut(s) 768, 804
BpuMI CCSGG 1 cut(s) 679
BsaJI CCNNGG 3 cut(s) 141, 306, 677
BsaWI WCCGGW 1 cut(s) 389
Bsc4I CCNNNNNNNGG 1 cut(s) 378
Bse118I RCCGGY 1 cut(s) 389
Bse1I ACTGG 1 cut(s) 621
BseBI CCWGG 3 cut(s) 271, 456, 791
BseDI CCNNGG 3 cut(s) 141, 306, 677
BseGI GGATG 1 cut(s) 838
BseLI CCNNNNNNNGG 1 cut(s) 378
BseMII CTCAG 2 cut(s) 786, 795
BseNI ACTGG 1 cut(s) 621
BseRI GAGGAG 2 cut(s) 275, 557
BseSI GKGCMC 1 cut(s) 677
BshFI GGCC 5 cut(s) 109, 140, 305, 509, 533
BshTI ACCGGT 1 cut(s) 389
BsiHKCI CYCGRG 1 cut(s) 757
BsiSI CCGG 2 cut(s) 390, 679
BslI CCNNNNNNNGG 1 cut(s) 378
BsnI GGCC 5 cut(s) 109, 140, 305, 509, 533
BsoBI CYCGRG 1 cut(s) 757
Bsp1286I GDGCHC 1 cut(s) 677
Bsp143I GATC 4 cut(s) 60, 124, 162, 753
Bsp19I CCATGG 1 cut(s) 141
BspACI CCGC 4 cut(s) 14, 225, 235, 382
BspANI GGCC 5 cut(s) 109, 140, 305, 509, 533
BspCNI CTCAG 2 cut(s) 787, 796
BspLI GGNNCC 2 cut(s) 585, 735
BsrFI RCCGGY 1 cut(s) 389
BsrI ACTGG 1 cut(s) 621
BssAI RCCGGY 1 cut(s) 389
BssECI CCNNGG 3 cut(s) 141, 306, 677
BssMI GATC 4 cut(s) 60, 124, 162, 753
BssNAI GTATAC 1 cut(s) 387
BssT1I CCWWGG 2 cut(s) 141, 306
Bst1107I GTATAC 1 cut(s) 387
Bst2UI CCWGG 3 cut(s) 271, 456, 791
Bst4CI ACNGT 2 cut(s) 443, 595
Bst6I CTCTTC 2 cut(s) 41, 282
BstC8I GCNNGC 1 cut(s) 531
BstDEI CTNAG 3 cut(s) 768, 795, 804
BstDSI CCRYGG 1 cut(s) 141
BstEII GGTNACC 1 cut(s) 265
BstF5I GGATG 1 cut(s) 838
BstKTI GATC 4 cut(s) 63, 127, 165, 756
BstMBI GATC 4 cut(s) 60, 124, 162, 753
BstMWI GCNNNNNNNGC 3 cut(s) 72, 231, 564
BstNI CCWGG 3 cut(s) 271, 456, 791
BstPI GGTNACC 1 cut(s) 265
BstSCI CCNGG 4 cut(s) 269, 454, 677, 789
BstSFI CTRYAG 1 cut(s) 496
BstSLI GKGCMC 1 cut(s) 677
BstXI CCANNNNNNTGG 1 cut(s) 580
BstZ17I GTATAC 1 cut(s) 387
BsuRI GGCC 5 cut(s) 109, 140, 305, 509, 533
BtgI CCRYGG 1 cut(s) 141
BtgZI GCGATG 1 cut(s) 476
BtsCI GGATG 1 cut(s) 838
BtsIMutI CAGTG 2 cut(s) 439, 628
Cac8I GCNNGC 1 cut(s) 531
Cfr10I RCCGGY 1 cut(s) 389
Cfr13I GGNCC 1 cut(s) 108
CsiI ACCWGGT 1 cut(s) 454
Csp6I GTAC 1 cut(s) 577
CspAI ACCGGT 1 cut(s) 389
CviAII CATG 4 cut(s) 142, 318, 356, 574
CviQI GTAC 1 cut(s) 577
DdeI CTNAG 3 cut(s) 768, 795, 804
DpnI GATC 4 cut(s) 62, 126, 164, 755
DpnII GATC 4 cut(s) 60, 124, 162, 753
EaeI YGGCCR 2 cut(s) 507, 531
Eam1104I CTCTTC 2 cut(s) 41, 282
EarI CTCTTC 2 cut(s) 41, 282
EciI GGCGGA 1 cut(s) 29
Eco130I CCWWGG 2 cut(s) 141, 306
Eco88I CYCGRG 1 cut(s) 757
Eco91I GGTNACC 1 cut(s) 265
EcoO109I RGGNCCY 1 cut(s) 108
EcoO65I GGTNACC 1 cut(s) 265
EcoRII CCWGG 3 cut(s) 269, 454, 789
EcoT14I CCWWGG 2 cut(s) 141, 306
ErhI CCWWGG 2 cut(s) 141, 306
FaeI CATG 4 cut(s) 145, 321, 359, 577
FatI CATG 4 cut(s) 141, 317, 355, 573
FauNDI CATATG 2 cut(s) 129, 629
FblI GTMKAC 2 cut(s) 386, 597
Fnu4HI GCNGC 1 cut(s) 235
FokI GGATG 1 cut(s) 825
Fsp4HI GCNGC 1 cut(s) 235
FspBI CTAG 2 cut(s) 333, 779
GluI GCNGC 1 cut(s) 235
GsuI CTGGAG 4 cut(s) 215, 292, 580, 843
HaeIII GGCC 5 cut(s) 109, 140, 305, 509, 533
HapII CCGG 2 cut(s) 390, 679
Hin1II CATG 4 cut(s) 145, 321, 359, 577
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HinfI GANTC 5 cut(s) 170, 176, 368, 682, 760
HpaII CCGG 2 cut(s) 390, 679
HphI GGTGA 4 cut(s) 112, 269, 404, 530
Hpy166II GTNNAC 4 cut(s) 387, 439, 598, 816
Hpy188I TCNGA 2 cut(s) 175, 373
Hpy188III TCNNGA 8 cut(s) 401, 551, 587, 653, 746, 757, 779, 803
Hpy8I GTNNAC 4 cut(s) 387, 439, 598, 816
HpyAV CCTTC 6 cut(s) 4, 62, 130, 398, 532, 853
HpyCH4III ACNGT 2 cut(s) 443, 595
HpyCH4V TGCA 2 cut(s) 95, 364
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 231, 564
HpyF3I CTNAG 3 cut(s) 768, 795, 804
Hsp92II CATG 4 cut(s) 145, 321, 359, 577
Kzo9I GATC 4 cut(s) 60, 124, 162, 753
LmnI GCTCC 2 cut(s) 589, 733
MabI ACCWGGT 1 cut(s) 454
MaeI CTAG 2 cut(s) 333, 779
MaeIII GTNAC 4 cut(s) 265, 458, 518, 623
MalI GATC 4 cut(s) 62, 126, 164, 755
MboI GATC 4 cut(s) 60, 124, 162, 753
MboII GAAGA 3 cut(s) 16, 58, 299
MhlI GDGCHC 1 cut(s) 677
MlsI TGGCCA 2 cut(s) 509, 533
MluCI AATT 1 cut(s) 741
MluNI TGGCCA 2 cut(s) 509, 533
MlyI GAGTC 1 cut(s) 769
Mox20I TGGCCA 2 cut(s) 509, 533
MroXI GAANNNNTTC 1 cut(s) 686
MscI TGGCCA 2 cut(s) 509, 533
MseI TTAA 1 cut(s) 411
MslI CAYNNNNRTG 5 cut(s) 146, 255, 426, 572, 690
Msp20I TGGCCA 2 cut(s) 509, 533
MspA1I CMGCKG 1 cut(s) 821
MspI CCGG 2 cut(s) 390, 679
MspR9I CCNGG 4 cut(s) 271, 456, 679, 791
MvaI CCWGG 3 cut(s) 271, 456, 791
MwoI GCNNNNNNNGC 3 cut(s) 72, 231, 564
NciI CCSGG 1 cut(s) 679
NcoI CCATGG 1 cut(s) 141
NdeI CATATG 2 cut(s) 129, 629
NdeII GATC 4 cut(s) 60, 124, 162, 753
NlaIII CATG 4 cut(s) 145, 321, 359, 577
NlaIV GGNNCC 2 cut(s) 585, 735
NmuCI GTSAC 2 cut(s) 518, 623
OliI CACNNNNGTG 1 cut(s) 426
PaeR7I CTCGAG 1 cut(s) 757
PdmI GAANNNNTTC 1 cut(s) 686
PfeI GAWTC 4 cut(s) 170, 176, 368, 682
PinAI ACCGGT 1 cut(s) 389
PkrI GCNGC 1 cut(s) 236
PleI GAGTC 1 cut(s) 768
PpsI GAGTC 1 cut(s) 768
Psp6I CCWGG 3 cut(s) 269, 454, 789
PspEI GGTNACC 1 cut(s) 265
PspGI CCWGG 3 cut(s) 269, 454, 789
PspN4I GGNNCC 2 cut(s) 585, 735
PspPI GGNCC 1 cut(s) 108
PvuII CAGCTG 1 cut(s) 821
RsaI GTAC 1 cut(s) 578
RsaNI GTAC 1 cut(s) 577
RseI CAYNNNNRTG 5 cut(s) 146, 255, 426, 572, 690
SalI GTCGAC 1 cut(s) 596
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 1 cut(s) 235
Sau3AI GATC 4 cut(s) 60, 124, 162, 753
Sau96I GGNCC 1 cut(s) 108
SchI GAGTC 1 cut(s) 769
ScrFI CCNGG 4 cut(s) 271, 456, 679, 791
SduI GDGCHC 1 cut(s) 677
SexAI ACCWGGT 1 cut(s) 454
SfcI CTRYAG 1 cut(s) 496
Sfr274I CTCGAG 1 cut(s) 757
SlaI CTCGAG 1 cut(s) 757
SmiMI CAYNNNNRTG 5 cut(s) 146, 255, 426, 572, 690
SmlI CTYRAG 1 cut(s) 757
SmoI CTYRAG 1 cut(s) 757
Sse9I AATT 1 cut(s) 741
SsiI CCGC 4 cut(s) 14, 225, 235, 382
SspMI CTAG 2 cut(s) 333, 779
StyD4I CCNGG 4 cut(s) 269, 454, 677, 789
StyI CCWWGG 2 cut(s) 141, 306
TaaI ACNGT 2 cut(s) 443, 595
TaqI TCGA 3 cut(s) 184, 597, 758
TaqII GACCGA 1 cut(s) 341
TasI AATT 1 cut(s) 741
TatI WGTACW 1 cut(s) 576
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 4 cut(s) 170, 176, 368, 682
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 2 cut(s) 446, 628
TseFI GTSAC 2 cut(s) 518, 623
Tsp45I GTSAC 2 cut(s) 518, 623
TspDTI ATGAA 3 cut(s) 17, 229, 479
TspRI CASTG 2 cut(s) 446, 628
XapI RAATTY 1 cut(s) 741
XbaI TCTAGA 1 cut(s) 778
XcmI CCANNNNNNNNNTGG 1 cut(s) 722
XhoI CTCGAG 1 cut(s) 757
XmiI GTMKAC 2 cut(s) 386, 597
XmnI GAANNNNTTC 1 cut(s) 686
XspI CTAG 2 cut(s) 333, 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.