Rw2G029440

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001294
Physical Location & Seq
Reverse (-)
47705183 .. 47718124
12942 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G029440.1

Sequence Viewer

Length: 792 bp
ATGAGGCCCTATATCATCTCCGATCATATGCCAAAGGCCATGGCTATGAAATCAGCAAAGATCAAGGATTCCGATTCCATTCGATACGAGCTGGAGGTGTATAGAGAGTTGATTAACGAGGGCTGTTCTTGCTCCTCCATCATTGAGCACTATGGTGAGGGGGTAACACGGAGTGATGAGGGTGAAGAGATCTATAACTTGGCCTTGGAAATAGCATGGGGAAGCCTTGCTAAGTTGATAGAGAAGACCGGTCATGGGTTGGGAGAATACCATGTAAGGCGGTATACCCGTGATATTCTTAAAGGTATTAAACACATACACAAAAGTAGTGGGTATGTCCACTGTGATTTGAAACCAGATAACATCTTGCTTTTGAAAACTGATGATTATATTGATCGATATCTCTGTCCCTATGAGGCCAAAGTTGGTGACTTGGGGCTGGCCAAGAAGGAGGAGGAGGTTCAGGAACGCTGGAGAGGGACACCCATGTACTTGTCTCCTGAAGCTGTAGAAGACAATGTCCAAGAACGACCCAGTGACATATGGGCTGTGGGGTGTATTGTTCTGGAAATGCTTACGGGAGAGTGCCCCCGGAATCATTTCTATGATGGTGAGAGTCGTACTCTCCACATTGTTGATGGAATGGAGCCAAAAATTCCTGATACGATCTCGAGTCGAGCTAAGGACTTTCTACAGCTTTGCCTAGCTACGGTTCCTGAGCAAAGGTGGACAGCTGAAGACCTTCAATCTCATCCCTTCGTGGCAAATCTTGAATTAGATAGTGATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

263

Amino Acids

30.05

Weight (kDa)

5.06

Isoelectric Point (pI)

52.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 12 - 254 4e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 43 - 250 1.7e-19 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 284
AciI CCGC 1 cut(s) 280
AcoI YGGCCR 1 cut(s) 441
AcsI RAATTY 1 cut(s) 654
AcuI CTGAAG 2 cut(s) 522, 756
AdeI CACNNNGTG 1 cut(s) 173
AfaI GTAC 2 cut(s) 491, 622
AfiI CCNNNNNNNGG 2 cut(s) 255, 709
AgeI ACCGGT 1 cut(s) 248
AgsI TTSAA 4 cut(s) 352, 376, 746, 773
AleI CACNNNNGTG 1 cut(s) 153
AluBI AGCT 6 cut(s) 91, 506, 680, 697, 707, 734
AluI AGCT 6 cut(s) 91, 506, 680, 697, 707, 734
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 1 cut(s) 501
Ama87I CYCGRG 1 cut(s) 670
AoxI GGCC 5 cut(s) 5, 36, 201, 417, 441
ApoI RAATTY 1 cut(s) 654
AsiGI ACCGGT 1 cut(s) 248
Asp700I GAANNNNTTC 2 cut(s) 599, 741
AspS9I GGNCC 1 cut(s) 6
AsuC2I CCSGG 1 cut(s) 592
AsuHPI GGTGA 4 cut(s) 167, 194, 440, 623
AvaI CYCGRG 1 cut(s) 670
BaeGI GKGCMC 1 cut(s) 590
BalI TGGCCA 1 cut(s) 443
BbsI GAAGAC 3 cut(s) 251, 519, 744
Bbv12I GWGCWC 1 cut(s) 150
BccI CCATC 3 cut(s) 146, 602, 632
BcnI CCSGG 1 cut(s) 592
BcoDI GTCTC 1 cut(s) 501
BfaI CTAG 1 cut(s) 704
BfmI CTRYAG 2 cut(s) 507, 692
BglII AGATCT 1 cut(s) 189
Bme1390I CCNGG 1 cut(s) 592
BmeT110I CYCGRG 1 cut(s) 670
BmgT120I GGNCC 1 cut(s) 6
BmiI GGNNCC 2 cut(s) 648, 714
BmrFI CCNGG 1 cut(s) 592
BmrI ACTGGG 1 cut(s) 528
BmuI ACTGGG 1 cut(s) 528
BpiI GAAGAC 3 cut(s) 251, 519, 744
BplI GAGNNNNNCTC 2 cut(s) 607, 639
BpmI CTGGAG 2 cut(s) 113, 493
Bpu10I CCTNAGC 2 cut(s) 681, 717
BpuMI CCSGG 1 cut(s) 592
Bsa29I ATCGAT 1 cut(s) 397
BsaBI GATNNNNATC 1 cut(s) 399
BsaJI CCNNGG 3 cut(s) 39, 204, 590
BsaWI WCCGGW 1 cut(s) 248
Bsc4I CCNNNNNNNGG 2 cut(s) 255, 709
Bse118I RCCGGY 1 cut(s) 248
Bse1I ACTGG 1 cut(s) 534
Bse8I GATNNNNATC 1 cut(s) 399
BseCI ATCGAT 1 cut(s) 397
BseDI CCNNGG 3 cut(s) 39, 204, 590
BseGI GGATG 1 cut(s) 751
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 2 cut(s) 255, 709
BseMII CTCAG 1 cut(s) 708
BseNI ACTGG 1 cut(s) 534
BseRI GAGGAG 3 cut(s) 124, 467, 470
BseSI GKGCMC 1 cut(s) 590
BshFI GGCC 5 cut(s) 7, 38, 203, 419, 443
BshTI ACCGGT 1 cut(s) 248
BshVI ATCGAT 1 cut(s) 397
BsiHKAI GWGCWC 1 cut(s) 150
BsiHKCI CYCGRG 1 cut(s) 670
BsiSI CCGG 2 cut(s) 249, 592
BslFI GGGAC 2 cut(s) 393, 493
BslI CCNNNNNNNGG 2 cut(s) 255, 709
BsmAI GTCTC 1 cut(s) 501
BsmFI GGGAC 2 cut(s) 393, 493
BsnI GGCC 5 cut(s) 7, 38, 203, 419, 443
BsoBI CYCGRG 1 cut(s) 670
Bsp1286I GDGCHC 2 cut(s) 150, 590
Bsp143I GATC 5 cut(s) 22, 60, 189, 394, 666
Bsp19I CCATGG 1 cut(s) 39
BspACI CCGC 1 cut(s) 280
BspANI GGCC 5 cut(s) 7, 38, 203, 419, 443
BspCNI CTCAG 1 cut(s) 709
BspDI ATCGAT 1 cut(s) 397
BspLI GGNNCC 2 cut(s) 648, 714
BsrFI RCCGGY 1 cut(s) 248
BsrI ACTGG 1 cut(s) 534
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 3 cut(s) 39, 204, 590
BssMI GATC 5 cut(s) 22, 60, 189, 394, 666
BssNAI GTATAC 1 cut(s) 285
BssT1I CCWWGG 2 cut(s) 39, 204
Bst1107I GTATAC 1 cut(s) 285
Bst4CI ACNGT 2 cut(s) 344, 712
Bst6I CTCTTC 1 cut(s) 180
BstC8I GCNNGC 1 cut(s) 441
BstDEI CTNAG 3 cut(s) 231, 681, 717
BstDSI CCRYGG 1 cut(s) 39
BstF5I GGATG 1 cut(s) 751
BstKTI GATC 5 cut(s) 25, 63, 192, 397, 669
BstMAI GTCTC 1 cut(s) 501
BstMBI GATC 5 cut(s) 22, 60, 189, 394, 666
BstMWI GCNNNNNNNGC 1 cut(s) 129
BstSCI CCNGG 1 cut(s) 590
BstSFI CTRYAG 2 cut(s) 507, 692
BstSLI GKGCMC 1 cut(s) 590
BstV2I GAAGAC 3 cut(s) 251, 519, 744
BstX2I RGATCY 1 cut(s) 189
BstYI RGATCY 1 cut(s) 189
BstZ17I GTATAC 1 cut(s) 285
Bsu15I ATCGAT 1 cut(s) 397
BsuRI GGCC 5 cut(s) 7, 38, 203, 419, 443
BsuTUI ATCGAT 1 cut(s) 397
BtgI CCRYGG 1 cut(s) 39
BtsCI GGATG 1 cut(s) 751
BtsIMutI CAGTG 2 cut(s) 340, 541
Cac8I GCNNGC 1 cut(s) 441
Cfr10I RCCGGY 1 cut(s) 248
Cfr13I GGNCC 1 cut(s) 6
ClaI ATCGAT 1 cut(s) 397
Csp6I GTAC 2 cut(s) 490, 621
CspAI ACCGGT 1 cut(s) 248
CspCI CAANNNNNGTGG 2 cut(s) 310, 345
CviAII CATG 5 cut(s) 40, 216, 254, 272, 487
CviQI GTAC 2 cut(s) 490, 621
DdeI CTNAG 3 cut(s) 231, 681, 717
DpnI GATC 5 cut(s) 24, 62, 191, 396, 668
DpnII GATC 5 cut(s) 22, 60, 189, 394, 666
DraIII CACNNNGTG 1 cut(s) 173
EaeI YGGCCR 1 cut(s) 441
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
Eco130I CCWWGG 2 cut(s) 39, 204
Eco32I GATATC 1 cut(s) 401
Eco57I CTGAAG 2 cut(s) 522, 756
Eco88I CYCGRG 1 cut(s) 670
EcoO109I RGGNCCY 1 cut(s) 6
EcoRV GATATC 1 cut(s) 401
EcoT14I CCWWGG 2 cut(s) 39, 204
ErhI CCWWGG 2 cut(s) 39, 204
FaeI CATG 5 cut(s) 43, 219, 257, 275, 490
FaqI GGGAC 2 cut(s) 393, 493
FatI CATG 5 cut(s) 39, 215, 253, 271, 486
FauNDI CATATG 2 cut(s) 27, 542
FblI GTMKAC 1 cut(s) 284
FokI GGATG 1 cut(s) 738
FspBI CTAG 1 cut(s) 704
GsuI CTGGAG 2 cut(s) 113, 493
HaeIII GGCC 5 cut(s) 7, 38, 203, 419, 443
HapII CCGG 2 cut(s) 249, 592
Hin1II CATG 5 cut(s) 43, 219, 257, 275, 490
HinfI GANTC 5 cut(s) 68, 74, 595, 616, 673
HpaII CCGG 2 cut(s) 249, 592
HphI GGTGA 4 cut(s) 167, 194, 440, 623
Hpy166II GTNNAC 3 cut(s) 285, 340, 729
Hpy188I TCNGA 2 cut(s) 22, 73
Hpy188III TCNNGA 7 cut(s) 464, 500, 566, 659, 670, 716, 770
Hpy8I GTNNAC 3 cut(s) 285, 340, 729
HpyAV CCTTC 3 cut(s) 442, 752, 766
HpyCH4III ACNGT 2 cut(s) 344, 712
HpyF10VI GCNNNNNNNGC 1 cut(s) 129
HpyF3I CTNAG 3 cut(s) 231, 681, 717
Hsp92II CATG 5 cut(s) 43, 219, 257, 275, 490
Kzo9I GATC 5 cut(s) 22, 60, 189, 394, 666
LmnI GCTCC 2 cut(s) 137, 646
MaeI CTAG 1 cut(s) 704
MaeIII GTNAC 3 cut(s) 163, 428, 536
MalI GATC 5 cut(s) 24, 62, 191, 396, 668
MboI GATC 5 cut(s) 22, 60, 189, 394, 666
MboII GAAGA 4 cut(s) 197, 256, 524, 749
MflI RGATCY 1 cut(s) 189
MhlI GDGCHC 2 cut(s) 150, 590
MlsI TGGCCA 1 cut(s) 443
MluCI AATT 3 cut(s) 654, 773, 787
MluNI TGGCCA 1 cut(s) 443
MlyI GAGTC 2 cut(s) 625, 682
Mox20I TGGCCA 1 cut(s) 443
MroXI GAANNNNTTC 2 cut(s) 599, 741
MscI TGGCCA 1 cut(s) 443
MseI TTAA 3 cut(s) 114, 300, 309
MslI CAYNNNNRTG 3 cut(s) 44, 153, 603
Msp20I TGGCCA 1 cut(s) 443
MspA1I CMGCKG 1 cut(s) 734
MspI CCGG 2 cut(s) 249, 592
MspR9I CCNGG 1 cut(s) 592
MwoI GCNNNNNNNGC 1 cut(s) 129
NciI CCSGG 1 cut(s) 592
NcoI CCATGG 1 cut(s) 39
NdeI CATATG 2 cut(s) 27, 542
NdeII GATC 5 cut(s) 22, 60, 189, 394, 666
NlaIII CATG 5 cut(s) 43, 219, 257, 275, 490
NlaIV GGNNCC 2 cut(s) 648, 714
NmuCI GTSAC 2 cut(s) 428, 536
OliI CACNNNNGTG 1 cut(s) 153
PaeR7I CTCGAG 1 cut(s) 670
PdmI GAANNNNTTC 2 cut(s) 599, 741
PfeI GAWTC 3 cut(s) 68, 74, 595
PflFI GACNNNGTC 1 cut(s) 518
PinAI ACCGGT 1 cut(s) 248
PleI GAGTC 2 cut(s) 624, 681
PpsI GAGTC 2 cut(s) 624, 681
PspN4I GGNNCC 2 cut(s) 648, 714
PspPI GGNCC 1 cut(s) 6
PsuI RGATCY 1 cut(s) 189
PsyI GACNNNGTC 1 cut(s) 518
PvuII CAGCTG 1 cut(s) 734
RsaI GTAC 2 cut(s) 491, 622
RsaNI GTAC 2 cut(s) 490, 621
RseI CAYNNNNRTG 3 cut(s) 44, 153, 603
SaqAI TTAA 3 cut(s) 114, 300, 309
Sau3AI GATC 5 cut(s) 22, 60, 189, 394, 666
Sau96I GGNCC 1 cut(s) 6
SchI GAGTC 2 cut(s) 625, 682
ScrFI CCNGG 1 cut(s) 592
SduI GDGCHC 2 cut(s) 150, 590
SfcI CTRYAG 2 cut(s) 507, 692
Sfr274I CTCGAG 1 cut(s) 670
SlaI CTCGAG 1 cut(s) 670
SmiMI CAYNNNNRTG 3 cut(s) 44, 153, 603
SmlI CTYRAG 1 cut(s) 670
SmoI CTYRAG 1 cut(s) 670
Sse9I AATT 3 cut(s) 654, 773, 787
SsiI CCGC 1 cut(s) 280
SspMI CTAG 1 cut(s) 704
StyD4I CCNGG 1 cut(s) 590
StyI CCWWGG 2 cut(s) 39, 204
TaaI ACNGT 2 cut(s) 344, 712
TaqI TCGA 4 cut(s) 82, 397, 671, 676
TasI AATT 3 cut(s) 654, 773, 787
TatI WGTACW 1 cut(s) 489
TfiI GAWTC 3 cut(s) 68, 74, 595
Tru1I TTAA 3 cut(s) 114, 300, 309
Tru9I TTAA 3 cut(s) 114, 300, 309
TscAI CASTG 2 cut(s) 347, 541
TseFI GTSAC 2 cut(s) 428, 536
Tsp45I GTSAC 2 cut(s) 428, 536
TspDTI ATGAA 1 cut(s) 62
TspGWI ACGGA 1 cut(s) 184
TspRI CASTG 2 cut(s) 347, 541
Tth111I GACNNNGTC 1 cut(s) 518
XapI RAATTY 1 cut(s) 654
XcmI CCANNNNNNNNNTGG 1 cut(s) 635
XhoI CTCGAG 1 cut(s) 670
XmiI GTMKAC 1 cut(s) 284
XmnI GAANNNNTTC 2 cut(s) 599, 741
XspI CTAG 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.