Rw7G036850

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001294
Physical Location & Seq
Forward (+)
55809140 .. 55809673
534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G036850.1

Sequence Viewer

Length: 534 bp
ATGGCGGTAAAATCGGCCAAGATGAGGGACTCAGAGTCTATTCAATACGAGCTTCAGGTTTATAGGGACTTGAACAATCATGGTGATCGTTGCCCCTTCATTATTGAGCATTATGGCGAAGAGGTAACAAAGAGCGATCAGGGTGAAGAGGTTTACAACTTGGCGTTGGAAGTTGCCTGGGGAAGTCTTGCAAGGCTGATAAAGGTTTTCGGTCGTGGGTTGCCGGAATCCGATGTGCGGCGGTATACTTGTGACCTTCTTGAAGGGATTAGACACAATCACAAGTGTGGTTATGTCCAATGTGACTCGAAGCCACATAATATTCTTGTTGTGGAAAATGAAGATGATGATGTCTATAGGTTGGTGGCCAAAGTGGCTGATTTGGGGTTGGCTAAGACGAAGGATGAGGTTCAAGAAAGGTGGAGAGGCACACCTATGTACTTGTCTCCGGAGACTATACTTTATAATGAACAAGAGGAGCCAAGTGATATTTGGGCCTTGGGGTGCATTGTTCTTGAGATGCTCACCGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

177

Amino Acids

20.29

Weight (kDa)

4.84

Isoelectric Point (pI)

47.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 177 2.8e-26 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 4 - 176 1.1e-10 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 465
AccI GTMKAC 1 cut(s) 245
AccIII TCCGGA 1 cut(s) 448
AciI CCGC 3 cut(s) 5, 238, 241
AcoI YGGCCR 2 cut(s) 15, 366
AcuI CTGAAG 1 cut(s) 38
AfaI GTAC 1 cut(s) 440
AfiI CCNNNNNNNGG 2 cut(s) 24, 237
AgsI TTSAA 4 cut(s) 44, 73, 263, 413
AhdI GACNNNNNGTC 1 cut(s) 34
AjnI CCWGG 1 cut(s) 176
AleI CACNNNNGTG 1 cut(s) 285
AluBI AGCT 1 cut(s) 52
AluI AGCT 1 cut(s) 52
Alw26I GTCTC 2 cut(s) 446, 450
Aor13HI TCCGGA 1 cut(s) 448
AoxI GGCC 3 cut(s) 15, 366, 495
AspS9I GGNCC 1 cut(s) 495
AsuHPI GGTGA 3 cut(s) 95, 155, 517
BalI TGGCCA 1 cut(s) 368
BciT130I CCWGG 1 cut(s) 178
BcoDI GTCTC 2 cut(s) 446, 450
BfmI CTRYAG 1 cut(s) 355
BglI GCCNNNNNGGC 1 cut(s) 374
BisI GCNGC 1 cut(s) 239
BlsI GCNGC 1 cut(s) 240
Bme1390I CCNGG 1 cut(s) 178
BmeRI GACNNNNNGTC 1 cut(s) 34
BmgT120I GGNCC 1 cut(s) 495
BmiI GGNNCC 1 cut(s) 480
BmrFI CCNGG 1 cut(s) 178
BmsI GCATC 1 cut(s) 510
BsaJI CCNNGG 2 cut(s) 177, 498
BsaWI WCCGGW 2 cut(s) 448, 527
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 237
BseAI TCCGGA 1 cut(s) 448
BseBI CCWGG 1 cut(s) 178
BseDI CCNNGG 2 cut(s) 177, 498
BseGI GGATG 1 cut(s) 409
BseLI CCNNNNNNNGG 2 cut(s) 24, 237
BseMII CTCAG 1 cut(s) 45
BseRI GAGGAG 1 cut(s) 491
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 3 cut(s) 17, 368, 497
BsiEI CGRYCG 1 cut(s) 214
BsiSI CCGG 3 cut(s) 224, 449, 528
BslFI GGGAC 2 cut(s) 41, 80
BslI CCNNNNNNNGG 2 cut(s) 24, 237
BsmAI GTCTC 2 cut(s) 446, 450
BsmFI GGGAC 2 cut(s) 41, 80
BsnI GGCC 3 cut(s) 17, 368, 497
Bsp13I TCCGGA 1 cut(s) 448
Bsp143I GATC 2 cut(s) 85, 136
BspACI CCGC 3 cut(s) 5, 238, 241
BspANI GGCC 3 cut(s) 17, 368, 497
BspCNI CTCAG 1 cut(s) 44
BspEI TCCGGA 1 cut(s) 448
BspLI GGNNCC 1 cut(s) 480
BssECI CCNNGG 2 cut(s) 177, 498
BssMI GATC 2 cut(s) 85, 136
BssNAI GTATAC 1 cut(s) 246
BssT1I CCWWGG 1 cut(s) 498
Bst1107I GTATAC 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 178
Bst6I CTCTTC 2 cut(s) 114, 141
BstDEI CTNAG 2 cut(s) 31, 393
BstF5I GGATG 1 cut(s) 409
BstKTI GATC 2 cut(s) 88, 139
BstMAI GTCTC 2 cut(s) 446, 450
BstMBI GATC 2 cut(s) 85, 136
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 1 cut(s) 374
BstNI CCWGG 1 cut(s) 178
BstSCI CCNGG 1 cut(s) 176
BstSFI CTRYAG 1 cut(s) 355
BstZ17I GTATAC 1 cut(s) 246
BsuRI GGCC 3 cut(s) 17, 368, 497
BtsCI GGATG 1 cut(s) 409
Cfr13I GGNCC 1 cut(s) 495
Csp6I GTAC 1 cut(s) 439
CspCI CAANNNNNGTGG 2 cut(s) 401, 436
CviAII CATG 1 cut(s) 80
CviJI RGCY 9 cut(s) 17, 52, 196, 313, 368, 377, 392, 481, 497
CviKI_1 RGCY 9 cut(s) 17, 52, 196, 313, 368, 377, 392, 481, 497
CviQI GTAC 1 cut(s) 439
DdeI CTNAG 2 cut(s) 31, 393
DpnI GATC 2 cut(s) 87, 138
DpnII GATC 2 cut(s) 85, 136
DriI GACNNNNNGTC 1 cut(s) 34
EaeI YGGCCR 2 cut(s) 15, 366
Eam1104I CTCTTC 2 cut(s) 114, 141
Eam1105I GACNNNNNGTC 1 cut(s) 34
EarI CTCTTC 2 cut(s) 114, 141
Eco130I CCWWGG 1 cut(s) 498
Eco57I CTGAAG 1 cut(s) 38
EcoRII CCWGG 1 cut(s) 176
EcoT14I CCWWGG 1 cut(s) 498
ErhI CCWWGG 1 cut(s) 498
FaeI CATG 1 cut(s) 83
FaqI GGGAC 2 cut(s) 41, 80
FatI CATG 1 cut(s) 79
FblI GTMKAC 1 cut(s) 245
Fnu4HI GCNGC 1 cut(s) 239
FokI GGATG 1 cut(s) 416
Fsp4HI GCNGC 1 cut(s) 239
GluI GCNGC 1 cut(s) 239
HaeIII GGCC 3 cut(s) 17, 368, 497
HapII CCGG 3 cut(s) 224, 449, 528
Hin1II CATG 1 cut(s) 83
HinfI GANTC 4 cut(s) 29, 35, 227, 305
HpaII CCGG 3 cut(s) 224, 449, 528
HphI GGTGA 3 cut(s) 95, 155, 517
Hpy166II GTNNAC 2 cut(s) 154, 246
Hpy188I TCNGA 2 cut(s) 34, 232
Hpy188III TCNNGA 4 cut(s) 260, 413, 449, 515
Hpy8I GTNNAC 2 cut(s) 154, 246
HpyAV CCTTC 4 cut(s) 106, 257, 266, 394
HpyCH4V TGCA 2 cut(s) 191, 507
HpyF10VI GCNNNNNNNGC 1 cut(s) 374
HpyF3I CTNAG 2 cut(s) 31, 393
Hsp92II CATG 1 cut(s) 83
Kpn2I TCCGGA 1 cut(s) 448
Kzo9I GATC 2 cut(s) 85, 136
LmnI GCTCC 1 cut(s) 478
LpnPI CCDG 6 cut(s) 41, 125, 163, 190, 237, 462
LweI GCATC 1 cut(s) 510
MaeIII GTNAC 3 cut(s) 124, 251, 302
MalI GATC 2 cut(s) 87, 138
MboI GATC 2 cut(s) 85, 136
MboII GAAGA 3 cut(s) 131, 158, 353
MlsI TGGCCA 1 cut(s) 368
MluNI TGGCCA 1 cut(s) 368
MlyI GAGTC 3 cut(s) 23, 44, 299
MmeI TCCRAC 1 cut(s) 147
MnlI CCTC 6 cut(s) 18, 115, 142, 400, 419, 469
Mox20I TGGCCA 1 cut(s) 368
MroI TCCGGA 1 cut(s) 448
MscI TGGCCA 1 cut(s) 368
MslI CAYNNNNRTG 2 cut(s) 285, 434
Msp20I TGGCCA 1 cut(s) 368
MspI CCGG 3 cut(s) 224, 449, 528
MspR9I CCNGG 1 cut(s) 178
MvaI CCWGG 1 cut(s) 178
MwoI GCNNNNNNNGC 1 cut(s) 374
NdeII GATC 2 cut(s) 85, 136
NlaIII CATG 1 cut(s) 83
NlaIV GGNNCC 1 cut(s) 480
NmuCI GTSAC 2 cut(s) 251, 302
OliI CACNNNNGTG 1 cut(s) 285
PfeI GAWTC 1 cut(s) 227
PkrI GCNGC 1 cut(s) 240
PleI GAGTC 3 cut(s) 23, 43, 299
PpsI GAGTC 3 cut(s) 23, 43, 299
PsiI TTATAA 1 cut(s) 465
Psp6I CCWGG 1 cut(s) 176
PspGI CCWGG 1 cut(s) 176
PspN4I GGNNCC 1 cut(s) 480
PspPI GGNCC 1 cut(s) 495
RsaI GTAC 1 cut(s) 440
RsaNI GTAC 1 cut(s) 439
RseI CAYNNNNRTG 2 cut(s) 285, 434
SatI GCNGC 1 cut(s) 239
Sau3AI GATC 2 cut(s) 85, 136
Sau96I GGNCC 1 cut(s) 495
SchI GAGTC 3 cut(s) 23, 44, 299
ScrFI CCNGG 1 cut(s) 178
SfaNI GCATC 1 cut(s) 510
SfcI CTRYAG 1 cut(s) 355
SmiMI CAYNNNNRTG 2 cut(s) 285, 434
SmlI CTYRAG 1 cut(s) 515
SmoI CTYRAG 1 cut(s) 515
SsiI CCGC 3 cut(s) 5, 238, 241
SspI AATATT 1 cut(s) 322
StyD4I CCNGG 1 cut(s) 176
StyI CCWWGG 1 cut(s) 498
TaqI TCGA 1 cut(s) 308
TaqII GACCGA 1 cut(s) 200
TatI WGTACW 1 cut(s) 438
TauI GCSGC 1 cut(s) 241
TfiI GAWTC 1 cut(s) 227
TseFI GTSAC 2 cut(s) 251, 302
Tsp45I GTSAC 2 cut(s) 251, 302
TspDTI ATGAA 3 cut(s) 88, 354, 483
XcmI CCANNNNNNNNNTGG 1 cut(s) 489
XmiI GTMKAC 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.