Rh1AG272100

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0001294
Physical Location & Seq
Reverse (-)
50033488 .. 50034228
741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG272100.1

Sequence Viewer

Length: 492 bp
ATGCCCGTGGCCATGGCCATGAAATCGGCAAAGATCAAGGATTCAGATTCCATTCGATACGAGCTCGAGGTGTTTAGGGAGTTGAATAACAAGGGTGGTTCTTGCCCCTTGATCATTGAGCACTATGGTGAAGAGGTGACATGGAGTGACGACGGGGAAGAGGTCTATAACTTGGCCTTGGAAGTTGCATGGGGAAGCCTTGCGAGGCTGATAAAGATTATCGGTCATGGGTTGCCAGAATCCGATGTGAGGCGGTACACCGGCGACATTCTTGAAGGGGTTCGACACCTACACAAGAGTGGGAATCATTTCTACGATCATGAGAGCGATACTCTCTACGTTGTTGATGGAATGGAGCCTAAAATTCCTGATACGATCTCGAGTCTTGCTAGGGAGTTTCTAGGGTTTTGCCTAGCCAAGGAAACTCCGGAAAGGTGGACAGCTGAAGAGCTTCTATCTCATCCCTTTGTGGCAAATGTTGTTAGGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

163

Amino Acids

18.48

Weight (kDa)

5.03

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 427
AciI CCGC 1 cut(s) 253
AcoI YGGCCR 2 cut(s) 9, 15
AcsI RAATTY 1 cut(s) 363
AcuI CTGAAG 1 cut(s) 465
AfaI GTAC 2 cut(s) 257, 488
AfiI CCNNNNNNNGG 2 cut(s) 249, 418
AgsI TTSAA 2 cut(s) 85, 275
AleI CACNNNNGTG 2 cut(s) 126, 297
AluBI AGCT 3 cut(s) 64, 443, 451
AluI AGCT 3 cut(s) 64, 443, 451
Alw21I GWGCWC 2 cut(s) 66, 123
Ama87I CYCGRG 2 cut(s) 65, 379
Aor13HI TCCGGA 1 cut(s) 427
AoxI GGCC 3 cut(s) 9, 15, 174
ApoI RAATTY 1 cut(s) 363
Asp700I GAANNNNTTC 3 cut(s) 279, 308, 450
AsuHPI GGTGA 2 cut(s) 140, 148
AvaI CYCGRG 2 cut(s) 65, 379
BalI TGGCCA 2 cut(s) 11, 17
BanII GRGCYC 1 cut(s) 66
Bbv12I GWGCWC 2 cut(s) 66, 123
BccI CCATC 1 cut(s) 341
BclI TGATCA 1 cut(s) 111
BfaI CTAG 3 cut(s) 390, 401, 413
BmeT110I CYCGRG 2 cut(s) 65, 379
BmiI GGNNCC 1 cut(s) 357
BplI GAGNNNNNCTC 2 cut(s) 316, 348
BsaJI CCNNGG 4 cut(s) 6, 12, 177, 417
BsaWI WCCGGW 1 cut(s) 427
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 418
Bse118I RCCGGY 1 cut(s) 260
BseAI TCCGGA 1 cut(s) 427
BseDI CCNNGG 4 cut(s) 6, 12, 177, 417
BseGI GGATG 1 cut(s) 460
BseLI CCNNNNNNNGG 2 cut(s) 249, 418
BshFI GGCC 3 cut(s) 11, 17, 176
BsiHKAI GWGCWC 2 cut(s) 66, 123
BsiHKCI CYCGRG 2 cut(s) 65, 379
BsiSI CCGG 2 cut(s) 261, 428
BslI CCNNNNNNNGG 2 cut(s) 249, 418
BsnI GGCC 3 cut(s) 11, 17, 176
BsoBI CYCGRG 2 cut(s) 65, 379
Bsp1286I GDGCHC 2 cut(s) 66, 123
Bsp13I TCCGGA 1 cut(s) 427
Bsp143I GATC 4 cut(s) 33, 111, 316, 375
Bsp19I CCATGG 1 cut(s) 12
BspACI CCGC 1 cut(s) 253
BspANI GGCC 3 cut(s) 11, 17, 176
BspEI TCCGGA 1 cut(s) 427
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 1 cut(s) 357
BspQI GCTCTTC 1 cut(s) 441
BsrFI RCCGGY 1 cut(s) 260
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 4 cut(s) 6, 12, 177, 417
BssMI GATC 4 cut(s) 33, 111, 316, 375
BssT1I CCWWGG 3 cut(s) 12, 177, 417
Bst6I CTCTTC 3 cut(s) 126, 153, 441
BstDSI CCRYGG 2 cut(s) 6, 12
BstENI CCTNNNNNAGG 1 cut(s) 416
BstF5I GGATG 1 cut(s) 460
BstKTI GATC 4 cut(s) 36, 114, 319, 378
BstMBI GATC 4 cut(s) 33, 111, 316, 375
BsuRI GGCC 3 cut(s) 11, 17, 176
BtgI CCRYGG 2 cut(s) 6, 12
BtsCI GGATG 1 cut(s) 460
CciI TCATGA 1 cut(s) 319
Cfr10I RCCGGY 1 cut(s) 260
Csp6I GTAC 2 cut(s) 256, 487
CviAII CATG 6 cut(s) 13, 19, 141, 189, 227, 320
CviQI GTAC 2 cut(s) 256, 487
DpnI GATC 4 cut(s) 35, 113, 318, 377
DpnII GATC 4 cut(s) 33, 111, 316, 375
EaeI YGGCCR 2 cut(s) 9, 15
Eam1104I CTCTTC 3 cut(s) 126, 153, 441
EarI CTCTTC 3 cut(s) 126, 153, 441
Ecl136II GAGCTC 1 cut(s) 64
Eco130I CCWWGG 3 cut(s) 12, 177, 417
Eco24I GRGCYC 1 cut(s) 66
Eco53kI GAGCTC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 465
Eco88I CYCGRG 2 cut(s) 65, 379
EcoICRI GAGCTC 1 cut(s) 64
EcoNI CCTNNNNNAGG 1 cut(s) 416
EcoT14I CCWWGG 3 cut(s) 12, 177, 417
EcoT38I GRGCYC 1 cut(s) 66
ErhI CCWWGG 3 cut(s) 12, 177, 417
FaeI CATG 6 cut(s) 16, 22, 144, 192, 230, 323
FaiI YATR 8 cut(s) 14, 20, 126, 142, 168, 190, 228, 321
FatI CATG 6 cut(s) 12, 18, 140, 188, 226, 319
FbaI TGATCA 1 cut(s) 111
FokI GGATG 1 cut(s) 447
FriOI GRGCYC 1 cut(s) 66
FspBI CTAG 3 cut(s) 390, 401, 413
HaeIII GGCC 3 cut(s) 11, 17, 176
HapII CCGG 2 cut(s) 261, 428
Hin1II CATG 6 cut(s) 16, 22, 144, 192, 230, 323
HinfI GANTC 5 cut(s) 41, 47, 239, 304, 382
HpaII CCGG 2 cut(s) 261, 428
HphI GGTGA 2 cut(s) 140, 148
Hpy166II GTNNAC 2 cut(s) 258, 438
Hpy188I TCNGA 2 cut(s) 46, 244
Hpy188III TCNNGA 5 cut(s) 272, 320, 368, 379, 428
Hpy8I GTNNAC 2 cut(s) 258, 438
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 1 cut(s) 269
HpyCH4IV ACGT 1 cut(s) 339
HpyCH4V TGCA 1 cut(s) 188
HpySE526I ACGT 1 cut(s) 339
Hsp92II CATG 6 cut(s) 16, 22, 144, 192, 230, 323
Kpn2I TCCGGA 1 cut(s) 427
Ksp22I TGATCA 1 cut(s) 111
Kzo9I GATC 4 cut(s) 33, 111, 316, 375
LguI GCTCTTC 1 cut(s) 441
LmnI GCTCC 1 cut(s) 355
LpnPI CCDG 4 cut(s) 249, 274, 381, 441
MaeI CTAG 3 cut(s) 390, 401, 413
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 2 cut(s) 136, 146
MalI GATC 4 cut(s) 35, 113, 318, 377
MboI GATC 4 cut(s) 33, 111, 316, 375
MboII GAAGA 3 cut(s) 143, 170, 458
MhlI GDGCHC 2 cut(s) 66, 123
MlsI TGGCCA 2 cut(s) 11, 17
MluCI AATT 1 cut(s) 363
MluNI TGGCCA 2 cut(s) 11, 17
MlyI GAGTC 1 cut(s) 391
MnlI CCTC 5 cut(s) 61, 127, 154, 198, 243
Mox20I TGGCCA 2 cut(s) 11, 17
MroI TCCGGA 1 cut(s) 427
MroXI GAANNNNTTC 3 cut(s) 279, 308, 450
MscI TGGCCA 2 cut(s) 11, 17
MslI CAYNNNNRTG 3 cut(s) 17, 126, 297
Msp20I TGGCCA 2 cut(s) 11, 17
MspA1I CMGCKG 1 cut(s) 443
MspI CCGG 2 cut(s) 261, 428
NcoI CCATGG 1 cut(s) 12
NdeII GATC 4 cut(s) 33, 111, 316, 375
NlaIII CATG 6 cut(s) 16, 22, 144, 192, 230, 323
NlaIV GGNNCC 1 cut(s) 357
NmuCI GTSAC 2 cut(s) 136, 146
OliI CACNNNNGTG 2 cut(s) 126, 297
PaeR7I CTCGAG 2 cut(s) 65, 379
PagI TCATGA 1 cut(s) 319
PciSI GCTCTTC 1 cut(s) 441
PdmI GAANNNNTTC 3 cut(s) 279, 308, 450
PfeI GAWTC 4 cut(s) 41, 47, 239, 304
PleI GAGTC 1 cut(s) 390
PpsI GAGTC 1 cut(s) 390
Psp124BI GAGCTC 1 cut(s) 66
PspN4I GGNNCC 1 cut(s) 357
PspXI VCTCGAGB 1 cut(s) 65
PvuII CAGCTG 1 cut(s) 443
RsaI GTAC 2 cut(s) 257, 488
RsaNI GTAC 2 cut(s) 256, 487
RseI CAYNNNNRTG 3 cut(s) 17, 126, 297
SacI GAGCTC 1 cut(s) 66
SapI GCTCTTC 1 cut(s) 441
Sau3AI GATC 4 cut(s) 33, 111, 316, 375
SchI GAGTC 1 cut(s) 391
SduI GDGCHC 2 cut(s) 66, 123
Sfr274I CTCGAG 2 cut(s) 65, 379
SgrAI CRCCGGYG 1 cut(s) 260
SlaI CTCGAG 2 cut(s) 65, 379
SmiMI CAYNNNNRTG 3 cut(s) 17, 126, 297
SmlI CTYRAG 2 cut(s) 65, 379
SmoI CTYRAG 2 cut(s) 65, 379
Sse9I AATT 1 cut(s) 363
SsiI CCGC 1 cut(s) 253
SspMI CTAG 3 cut(s) 390, 401, 413
SstI GAGCTC 1 cut(s) 66
StyI CCWWGG 3 cut(s) 12, 177, 417
TaiI ACGT 1 cut(s) 342
TaqI TCGA 4 cut(s) 55, 66, 283, 380
TaqII GACCGA 1 cut(s) 212
TasI AATT 1 cut(s) 363
TfiI GAWTC 4 cut(s) 41, 47, 239, 304
TseFI GTSAC 2 cut(s) 136, 146
Tsp45I GTSAC 2 cut(s) 136, 146
TspDTI ATGAA 1 cut(s) 35
XagI CCTNNNNNAGG 1 cut(s) 416
XapI RAATTY 1 cut(s) 363
XhoI CTCGAG 2 cut(s) 65, 379
XmnI GAANNNNTTC 3 cut(s) 279, 308, 450
XspI CTAG 3 cut(s) 390, 401, 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.