Rmu_sc0004657.1_g000015

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004657.1
OG0001294
Physical Location & Seq
Forward (+)
43728 .. 44273
546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004657.1_g000015.1.cds

Sequence Viewer

Length: 546 bp
atgcctgaggccatggcggtaaaatcggccaagatgagggactcagagtctattcaatacgagcttcaggtttatagggacttgaacaatcatggtgatcgttgccccttcattattgagcattatggcgaagaggtaacaaagagcgatcagggtgaagaggtttacaacttggcgttggaagttgcctggggaagttttgcaaggctgataaaggttttcggtcgtgggttgccggaatccgatgtgcggcggtatacctgtgaccttcttgaagggattagacacatccacaagtgtggttatgtccaatgtgactcgaagccacataatattcttgttgtggaaaatgaagatgatgatgtctataggttggtggccaaagtggctgatttggggttggctaagacgaaggatgaggttcaagaaaggtggagaggcacacctatgtacttgtctccggagactatactttataatgaacaagaggagccaagtgatatttgggccttggggtgcattgttcttgagatgctcaccggatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

181

Amino Acids

20.76

Weight (kDa)

4.79

Isoelectric Point (pI)

51.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 477
AccI GTMKAC 1 cut(s) 257
AccIII TCCGGA 1 cut(s) 460
AciI CCGC 3 cut(s) 17, 250, 253
AcoI YGGCCR 2 cut(s) 27, 378
AcuI CTGAAG 1 cut(s) 50
AfaI GTAC 1 cut(s) 452
AfiI CCNNNNNNNGG 2 cut(s) 36, 249
AgsI TTSAA 4 cut(s) 56, 85, 275, 425
AhdI GACNNNNNGTC 1 cut(s) 46
AjnI CCWGG 1 cut(s) 188
AleI CACNNNNGTG 1 cut(s) 297
AluBI AGCT 1 cut(s) 64
AluI AGCT 1 cut(s) 64
Alw26I GTCTC 2 cut(s) 458, 462
Aor13HI TCCGGA 1 cut(s) 460
AoxI GGCC 4 cut(s) 9, 27, 378, 507
AspS9I GGNCC 1 cut(s) 507
AsuHPI GGTGA 3 cut(s) 107, 167, 529
AxyI CCTNAGG 1 cut(s) 6
BalI TGGCCA 1 cut(s) 380
BciT130I CCWGG 1 cut(s) 190
BcoDI GTCTC 2 cut(s) 458, 462
BfmI CTRYAG 1 cut(s) 367
BglI GCCNNNNNGGC 1 cut(s) 386
BisI GCNGC 1 cut(s) 251
BlsI GCNGC 1 cut(s) 252
Bme1390I CCNGG 1 cut(s) 190
BmeRI GACNNNNNGTC 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 507
BmiI GGNNCC 1 cut(s) 492
BmrFI CCNGG 1 cut(s) 190
BmsI GCATC 1 cut(s) 522
BsaJI CCNNGG 3 cut(s) 12, 189, 510
BsaWI WCCGGW 2 cut(s) 460, 539
Bsc4I CCNNNNNNNGG 2 cut(s) 36, 249
Bse21I CCTNAGG 1 cut(s) 6
BseAI TCCGGA 1 cut(s) 460
BseBI CCWGG 1 cut(s) 190
BseDI CCNNGG 3 cut(s) 12, 189, 510
BseGI GGATG 2 cut(s) 288, 421
BseLI CCNNNNNNNGG 2 cut(s) 36, 249
BseMII CTCAG 1 cut(s) 57
BseRI GAGGAG 1 cut(s) 503
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 4 cut(s) 11, 29, 380, 509
BsiEI CGRYCG 1 cut(s) 226
BsiSI CCGG 3 cut(s) 236, 461, 540
BslFI GGGAC 2 cut(s) 53, 92
BslI CCNNNNNNNGG 2 cut(s) 36, 249
BsmAI GTCTC 2 cut(s) 458, 462
BsmFI GGGAC 2 cut(s) 53, 92
BsnI GGCC 4 cut(s) 11, 29, 380, 509
Bsp13I TCCGGA 1 cut(s) 460
Bsp143I GATC 2 cut(s) 97, 148
Bsp19I CCATGG 1 cut(s) 12
BspACI CCGC 3 cut(s) 17, 250, 253
BspANI GGCC 4 cut(s) 11, 29, 380, 509
BspCNI CTCAG 1 cut(s) 56
BspEI TCCGGA 1 cut(s) 460
BspLI GGNNCC 1 cut(s) 492
BssECI CCNNGG 3 cut(s) 12, 189, 510
BssMI GATC 2 cut(s) 97, 148
BssNAI GTATAC 1 cut(s) 258
BssT1I CCWWGG 2 cut(s) 12, 510
Bst1107I GTATAC 1 cut(s) 258
Bst2UI CCWGG 1 cut(s) 190
Bst6I CTCTTC 2 cut(s) 126, 153
BstDEI CTNAG 3 cut(s) 6, 43, 405
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 2 cut(s) 288, 421
BstKTI GATC 2 cut(s) 100, 151
BstMAI GTCTC 2 cut(s) 458, 462
BstMBI GATC 2 cut(s) 97, 148
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 1 cut(s) 386
BstNI CCWGG 1 cut(s) 190
BstSCI CCNGG 1 cut(s) 188
BstSFI CTRYAG 1 cut(s) 367
BstXI CCANNNNNNTGG 1 cut(s) 299
BstZ17I GTATAC 1 cut(s) 258
Bsu36I CCTNAGG 1 cut(s) 6
BsuRI GGCC 4 cut(s) 11, 29, 380, 509
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 2 cut(s) 288, 421
Cfr13I GGNCC 1 cut(s) 507
Csp6I GTAC 1 cut(s) 451
CspCI CAANNNNNGTGG 2 cut(s) 413, 448
CviAII CATG 2 cut(s) 13, 92
CviQI GTAC 1 cut(s) 451
DdeI CTNAG 3 cut(s) 6, 43, 405
DpnI GATC 2 cut(s) 99, 150
DpnII GATC 2 cut(s) 97, 148
DriI GACNNNNNGTC 1 cut(s) 46
EaeI YGGCCR 2 cut(s) 27, 378
Eam1104I CTCTTC 2 cut(s) 126, 153
Eam1105I GACNNNNNGTC 1 cut(s) 46
EarI CTCTTC 2 cut(s) 126, 153
Eco130I CCWWGG 2 cut(s) 12, 510
Eco57I CTGAAG 1 cut(s) 50
Eco81I CCTNAGG 1 cut(s) 6
EcoRII CCWGG 1 cut(s) 188
EcoT14I CCWWGG 2 cut(s) 12, 510
ErhI CCWWGG 2 cut(s) 12, 510
FaeI CATG 2 cut(s) 16, 95
FaqI GGGAC 2 cut(s) 53, 92
FatI CATG 2 cut(s) 12, 91
FblI GTMKAC 1 cut(s) 257
Fnu4HI GCNGC 1 cut(s) 251
FokI GGATG 2 cut(s) 275, 428
Fsp4HI GCNGC 1 cut(s) 251
GluI GCNGC 1 cut(s) 251
HaeIII GGCC 4 cut(s) 11, 29, 380, 509
HapII CCGG 3 cut(s) 236, 461, 540
Hin1II CATG 2 cut(s) 16, 95
HinfI GANTC 4 cut(s) 41, 47, 239, 317
HpaII CCGG 3 cut(s) 236, 461, 540
HphI GGTGA 3 cut(s) 107, 167, 529
Hpy166II GTNNAC 2 cut(s) 166, 258
Hpy188I TCNGA 2 cut(s) 46, 244
Hpy188III TCNNGA 4 cut(s) 272, 425, 461, 527
Hpy8I GTNNAC 2 cut(s) 166, 258
HpyAV CCTTC 4 cut(s) 118, 269, 278, 406
HpyCH4V TGCA 2 cut(s) 203, 519
HpyF10VI GCNNNNNNNGC 1 cut(s) 386
HpyF3I CTNAG 3 cut(s) 6, 43, 405
Hsp92II CATG 2 cut(s) 16, 95
Kpn2I TCCGGA 1 cut(s) 460
Kzo9I GATC 2 cut(s) 97, 148
LmnI GCTCC 1 cut(s) 490
LpnPI CCDG 8 cut(s) 18, 53, 137, 175, 202, 249, 274, 474
LweI GCATC 1 cut(s) 522
MaeIII GTNAC 3 cut(s) 136, 263, 314
MalI GATC 2 cut(s) 99, 150
MboI GATC 2 cut(s) 97, 148
MboII GAAGA 3 cut(s) 143, 170, 365
MlsI TGGCCA 1 cut(s) 380
MluNI TGGCCA 1 cut(s) 380
MlyI GAGTC 3 cut(s) 35, 56, 311
MmeI TCCRAC 1 cut(s) 159
MnlI CCTC 6 cut(s) 30, 127, 154, 412, 431, 481
Mox20I TGGCCA 1 cut(s) 380
MroI TCCGGA 1 cut(s) 460
MscI TGGCCA 1 cut(s) 380
MslI CAYNNNNRTG 2 cut(s) 297, 446
Msp20I TGGCCA 1 cut(s) 380
MspI CCGG 3 cut(s) 236, 461, 540
MspR9I CCNGG 1 cut(s) 190
MvaI CCWGG 1 cut(s) 190
MwoI GCNNNNNNNGC 1 cut(s) 386
NcoI CCATGG 1 cut(s) 12
NdeII GATC 2 cut(s) 97, 148
NlaIII CATG 2 cut(s) 16, 95
NlaIV GGNNCC 1 cut(s) 492
NmuCI GTSAC 2 cut(s) 263, 314
OliI CACNNNNGTG 1 cut(s) 297
PfeI GAWTC 1 cut(s) 239
PkrI GCNGC 1 cut(s) 252
PleI GAGTC 3 cut(s) 35, 55, 311
PpsI GAGTC 3 cut(s) 35, 55, 311
PsiI TTATAA 1 cut(s) 477
Psp6I CCWGG 1 cut(s) 188
PspGI CCWGG 1 cut(s) 188
PspN4I GGNNCC 1 cut(s) 492
PspPI GGNCC 1 cut(s) 507
RsaI GTAC 1 cut(s) 452
RsaNI GTAC 1 cut(s) 451
RseI CAYNNNNRTG 2 cut(s) 297, 446
SatI GCNGC 1 cut(s) 251
Sau3AI GATC 2 cut(s) 97, 148
Sau96I GGNCC 1 cut(s) 507
SchI GAGTC 3 cut(s) 35, 56, 311
ScrFI CCNGG 1 cut(s) 190
SfaNI GCATC 1 cut(s) 522
SfcI CTRYAG 1 cut(s) 367
SmiMI CAYNNNNRTG 2 cut(s) 297, 446
SmlI CTYRAG 1 cut(s) 527
SmoI CTYRAG 1 cut(s) 527
SsiI CCGC 3 cut(s) 17, 250, 253
SspI AATATT 1 cut(s) 334
StyD4I CCNGG 1 cut(s) 188
StyI CCWWGG 2 cut(s) 12, 510
TaqI TCGA 1 cut(s) 320
TaqII GACCGA 1 cut(s) 212
TatI WGTACW 1 cut(s) 450
TauI GCSGC 1 cut(s) 253
TfiI GAWTC 1 cut(s) 239
TseFI GTSAC 2 cut(s) 263, 314
Tsp45I GTSAC 2 cut(s) 263, 314
TspDTI ATGAA 3 cut(s) 100, 366, 495
XcmI CCANNNNNNNNNTGG 1 cut(s) 501
XmiI GTMKAC 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.