Prupe.3G102600_v2.0.a1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001294
Physical Location & Seq
Reverse (-)
7958189 .. 7958800
612 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G102600.1

Sequence Viewer

Length: 570 bp
ATGCTAAACTTTCCGGGATTGATGGCGGTGAAATCGGCAGAGGTCTCTGATTCGGAGTCAATCCGGCATGAGGCAGAGGTTCTGTTGGATATTAAGGGTTGCCCTTTTATCATTGAGCGCTTTGGTGAAGAAATCACAACCAGTGAAGAGGGTGGTGATCACATGGTCTATAACTTGTTGCTGGAATTTGCCCCTGGAGGAACTCTTGGTGATCTGATTGAGAAATCCAACGGTCATGGATTGCCTGAAGCTGAAGTTAGAAGGTATACGAGGTCGATACTTGAAGGGATTAAGCATATTCACAAGTTTGATTGTGTTCACTGTGATTTTACTGGTAGTTTTGTGGCTAAGATTTCAGATTTTGGATTGGCTAAGAAAACCAAGGAGAATTGCGGTCAATGGAGAGGCATTCCTACGTATCTGTCCCCAGAAGCCTTGAATGATAGTGAGCAGGAGCCGCCCTCTGATATATGGTCTCTTGGTTGTATCGTACTTGAGATGCTAACCGGCAAGTCCTTCTCAGATTTAAAGCCTGGTTGTGAACTTGAAGATTCCCGAAATCCCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

190

Amino Acids

20.88

Weight (kDa)

4.79

Isoelectric Point (pI)

46.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 266
AciI CCGC 3 cut(s) 26, 393, 458
AcsI RAATTY 1 cut(s) 185
AcuI CTGAAG 2 cut(s) 267, 273
AfaI GTAC 1 cut(s) 492
AfeI AGCGCT 1 cut(s) 119
AfiI CCNNNNNNNGG 1 cut(s) 70
AgsI TTSAA 3 cut(s) 284, 439, 548
AjnI CCWGG 2 cut(s) 193, 532
AluBI AGCT 1 cut(s) 251
AluI AGCT 1 cut(s) 251
Alw26I GTCTC 2 cut(s) 49, 480
Aor51HI AGCGCT 1 cut(s) 119
ApoI RAATTY 1 cut(s) 185
AspLEI GCGC 1 cut(s) 120
AsuC2I CCSGG 1 cut(s) 15
AsuHPI GGTGA 4 cut(s) 40, 137, 167, 221
BccI CCATC 1 cut(s) 16
BcgI CGANNNNNNTGC 1 cut(s) 546
BciT130I CCWGG 2 cut(s) 195, 534
BclI TGATCA 1 cut(s) 157
BcnI CCSGG 1 cut(s) 15
BcoDI GTCTC 2 cut(s) 49, 480
BfoI RGCGCY 1 cut(s) 121
BisI GCNGC 1 cut(s) 458
BlsI GCNGC 1 cut(s) 459
Bme1390I CCNGG 3 cut(s) 15, 195, 534
BmiI GGNNCC 1 cut(s) 456
BmrFI CCNGG 3 cut(s) 15, 195, 534
BmsI GCATC 1 cut(s) 489
BplI GAGNNNNNCTC 2 cut(s) 446, 478
BpmI CTGGAG 1 cut(s) 216
BpuEI CTTGAG 1 cut(s) 515
BpuMI CCSGG 1 cut(s) 15
BsaAI YACGTR 1 cut(s) 417
BsaI GGTCTC 2 cut(s) 49, 480
BsaJI CCNNGG 2 cut(s) 193, 381
Bsc4I CCNNNNNNNGG 1 cut(s) 70
Bse118I RCCGGY 1 cut(s) 506
Bse1I ACTGG 2 cut(s) 141, 337
BseBI CCWGG 2 cut(s) 195, 534
BseDI CCNNGG 2 cut(s) 193, 381
BseLI CCNNNNNNNGG 1 cut(s) 70
BseMII CTCAG 1 cut(s) 534
BseNI ACTGG 2 cut(s) 141, 337
BsiSI CCGG 3 cut(s) 14, 64, 507
BslFI GGGAC 1 cut(s) 409
BslI CCNNNNNNNGG 1 cut(s) 70
BsmAI GTCTC 2 cut(s) 49, 480
BsmFI GGGAC 1 cut(s) 409
BsmI GAATGC 1 cut(s) 408
Bso31I GGTCTC 2 cut(s) 49, 480
Bsp143I GATC 2 cut(s) 157, 211
BspACI CCGC 3 cut(s) 26, 393, 458
BspCNI CTCAG 1 cut(s) 533
BspLI GGNNCC 1 cut(s) 456
BspTNI GGTCTC 2 cut(s) 49, 480
BsrFI RCCGGY 1 cut(s) 506
BsrI ACTGG 2 cut(s) 141, 337
BssAI RCCGGY 1 cut(s) 506
BssECI CCNNGG 2 cut(s) 193, 381
BssMI GATC 2 cut(s) 157, 211
BssNAI GTATAC 1 cut(s) 267
BssT1I CCWWGG 1 cut(s) 381
Bst1107I GTATAC 1 cut(s) 267
Bst2UI CCWGG 2 cut(s) 195, 534
Bst4CI ACNGT 2 cut(s) 233, 323
Bst6I CTCTTC 1 cut(s) 141
BstBAI YACGTR 1 cut(s) 417
BstDEI CTNAG 3 cut(s) 348, 372, 520
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 2 cut(s) 160, 214
BstMAI GTCTC 2 cut(s) 49, 480
BstMBI GATC 2 cut(s) 157, 211
BstMWI GCNNNNNNNGC 1 cut(s) 457
BstNI CCWGG 2 cut(s) 195, 534
BstSCI CCNGG 3 cut(s) 13, 193, 532
BstSNI TACGTA 1 cut(s) 417
BstZ17I GTATAC 1 cut(s) 267
BtsIMutI CAGTG 2 cut(s) 148, 319
CfoI GCGC 1 cut(s) 120
Cfr10I RCCGGY 1 cut(s) 506
Csp6I GTAC 1 cut(s) 491
CviAII CATG 3 cut(s) 68, 163, 236
CviJI RGCY 6 cut(s) 251, 347, 371, 434, 457, 532
CviKI_1 RGCY 6 cut(s) 251, 347, 371, 434, 457, 532
CviQI GTAC 1 cut(s) 491
DdeI CTNAG 3 cut(s) 348, 372, 520
DpnI GATC 2 cut(s) 159, 213
DpnII GATC 2 cut(s) 157, 211
DraI TTTAAA 1 cut(s) 528
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
Eco105I TACGTA 1 cut(s) 417
Eco130I CCWWGG 1 cut(s) 381
Eco31I GGTCTC 2 cut(s) 49, 480
Eco47III AGCGCT 1 cut(s) 119
Eco57I CTGAAG 2 cut(s) 267, 273
EcoRII CCWGG 2 cut(s) 193, 532
EcoT14I CCWWGG 1 cut(s) 381
ErhI CCWWGG 1 cut(s) 381
FaeI CATG 3 cut(s) 71, 166, 239
FaiI YATR 8 cut(s) 69, 164, 171, 237, 267, 297, 470, 472
FaqI GGGAC 1 cut(s) 409
FatI CATG 3 cut(s) 67, 162, 235
FbaI TGATCA 1 cut(s) 157
FblI GTMKAC 1 cut(s) 266
Fnu4HI GCNGC 1 cut(s) 458
Fsp4HI GCNGC 1 cut(s) 458
GlaI GCGC 1 cut(s) 119
GluI GCNGC 1 cut(s) 458
GsuI CTGGAG 1 cut(s) 216
HaeII RGCGCY 1 cut(s) 121
HapII CCGG 3 cut(s) 14, 64, 507
HhaI GCGC 1 cut(s) 120
Hin1II CATG 3 cut(s) 71, 166, 239
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HinfI GANTC 3 cut(s) 50, 56, 551
HpaII CCGG 3 cut(s) 14, 64, 507
HphI GGTGA 4 cut(s) 40, 137, 167, 221
Hpy166II GTNNAC 3 cut(s) 267, 319, 542
Hpy188I TCNGA 6 cut(s) 49, 55, 216, 358, 466, 523
Hpy188III TCNNGA 1 cut(s) 555
Hpy8I GTNNAC 3 cut(s) 267, 319, 542
HpyAV CCTTC 3 cut(s) 255, 278, 526
HpyCH4III ACNGT 2 cut(s) 233, 323
HpyCH4IV ACGT 1 cut(s) 416
HpyF10VI GCNNNNNNNGC 1 cut(s) 457
HpyF3I CTNAG 3 cut(s) 348, 372, 520
HpySE526I ACGT 1 cut(s) 416
Hsp92II CATG 3 cut(s) 71, 166, 239
HspAI GCGC 1 cut(s) 118
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 2 cut(s) 157, 211
LmnI GCTCC 1 cut(s) 454
LweI GCATC 1 cut(s) 489
MaeII ACGT 1 cut(s) 416
MalI GATC 2 cut(s) 159, 213
MboI GATC 2 cut(s) 157, 211
MboII GAAGA 3 cut(s) 140, 158, 560
MluCI AATT 2 cut(s) 185, 388
MlyI GAGTC 1 cut(s) 65
MmeI TCCRAC 2 cut(s) 66, 252
MnlI CCTC 8 cut(s) 34, 64, 70, 142, 191, 264, 398, 472
MseI TTAA 3 cut(s) 93, 291, 527
MspI CCGG 3 cut(s) 14, 64, 507
MspR9I CCNGG 3 cut(s) 15, 195, 534
Mva1269I GAATGC 1 cut(s) 408
MvaI CCWGG 2 cut(s) 195, 534
MwoI GCNNNNNNNGC 1 cut(s) 457
NciI CCSGG 1 cut(s) 15
NdeII GATC 2 cut(s) 157, 211
NlaIII CATG 3 cut(s) 71, 166, 239
NlaIV GGNNCC 1 cut(s) 456
PctI GAATGC 1 cut(s) 408
PfeI GAWTC 2 cut(s) 50, 551
PfoI TCCNGGA 1 cut(s) 13
PkrI GCNGC 1 cut(s) 459
PleI GAGTC 1 cut(s) 64
PpsI GAGTC 1 cut(s) 64
Ppu21I YACGTR 1 cut(s) 417
Psp6I CCWGG 2 cut(s) 193, 532
PspGI CCWGG 2 cut(s) 193, 532
PspN4I GGNNCC 1 cut(s) 456
RsaI GTAC 1 cut(s) 492
RsaNI GTAC 1 cut(s) 491
SaqAI TTAA 3 cut(s) 93, 291, 527
SatI GCNGC 1 cut(s) 458
Sau3AI GATC 2 cut(s) 157, 211
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 3 cut(s) 15, 195, 534
SetI ASST 6 cut(s) 45, 81, 253, 266, 275, 419
SfaNI GCATC 1 cut(s) 489
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
SnaBI TACGTA 1 cut(s) 417
Sse9I AATT 2 cut(s) 185, 388
SsiI CCGC 3 cut(s) 26, 393, 458
StyD4I CCNGG 3 cut(s) 13, 193, 532
StyI CCWWGG 1 cut(s) 381
TaaI ACNGT 2 cut(s) 233, 323
TaiI ACGT 1 cut(s) 419
TaqI TCGA 1 cut(s) 275
TasI AATT 2 cut(s) 185, 388
TauI GCSGC 1 cut(s) 460
TfiI GAWTC 2 cut(s) 50, 551
Tru1I TTAA 3 cut(s) 93, 291, 527
Tru9I TTAA 3 cut(s) 93, 291, 527
TscAI CASTG 2 cut(s) 148, 326
TspRI CASTG 2 cut(s) 148, 326
XapI RAATTY 1 cut(s) 185
XmiI GTMKAC 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.