Prupe.3G099000_v2.0.a1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001294
Physical Location & Seq
Reverse (-)
7612235 .. 7614957
2723 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G099000.1

Sequence Viewer

Length: 1293 bp
ATGGCAATGAAGAAAACTTCAGATGAAAGGAAGGCGTTGAAGTACTTGAAGCCAGATGGGGAAGAAGACCTCCTCTGCCTAAAAACAGAGCACAACCCAAAGGAGCCCATGAAGCTGACTTTGAGCCCATCTCAAGATCAAACCAAGGCTGAGCCATGGTTGTTCAAACCAAAACCTGGCAGTGTCTTCCCTATGAAGAAGAGTGAAGAAATCATGAAACGGAAGGCAGAGCAAGAATTAGAAGAAAGAGGGAAAAAGCAGAGGATGTGGTGGGGTGATGGAAATCAGTGGGAGAGAGGGGAGATGATCGGAGAAGGAAGCTATGGGTCTGTTTTTCTTGCTTTTTGGAAAAAACCCCTTTGGGAGCTTAATCTAAATGATACACCTCCAGCTCCAGTCATGGCAGTCAAATCTGCTAAGCTCTCTGATTCAGAATCCATTCAGCATGAGGCAGGCATTCTCTCTGATTTTATAGGGTCCCCTTTTGTAATTCAGTACTTGGGTGAAGAAATGACAACCATTGATGACACAGGTGAAGAAATATTCAACTTGGCTTTGGAATTTGCTGCTGGAGGAACCCTTGATGGTCTGATTCAGAGCTATAGAGGTCATGGGCTGCCTGAGTCTGATGTGAGGAGCTACACAAGGTGCATACTTGAAGGGCTTATACATATTCACAATTGTGATTATGTTCACTGTGATTTGAAGCCGGAGAACATCCTTCTTGTTCCTAGTGATACTAGCACCAGTGGTAGTTCTAGTTTTGTGGCCAAGATTGCTGATTTCGGATTGACTAAGAGCACCAAAGATGATATGGATGGATGGAGAGGCACTCCTAGGTATACAGACCCAGATGCTTTGATTGATAATGTGCAGAAGCAGTGTTCTGATATTTGGTCTCTTGGTGCTATTGTGCTTGAGATGTTAACAGGCAAGCCCCCCTGGGATGTGGAGCCTGATTCGACACTTGATGATTTCCTGGACATGGTTGCTGGGGAAATAACTCCCAAAATCCCTACTGAAATCTCTGGTTTGGCAAGGGATTTTCTTATGAAGTGTATTGCAGTCAACTCATGGGAAAGGTTTACCGCTCAAAAGCTCCTACTTCATCCATTTGTACAGGAGCCACAACTATCTGAACCAAGTCATGCCAAGGTGAAGGTGCTTAGCTCATCTTTGGGCTCTGAAGATGGTGCCTCTTGCTTGAAACCAATTGAAGATTACCGCACAAACTCTGCCCCTGTTCCCAGAATTCAGCCCTCACCGGGTTTTGAGATACCAGCTGGACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

431

Amino Acids

47.88

Weight (kDa)

4.93

Isoelectric Point (pI)

40.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1193
AccB7I CCANNNNNTGG 1 cut(s) 176
AccBSI CCGCTC 1 cut(s) 1091
AccI GTMKAC 1 cut(s) 842
AciI CCGC 2 cut(s) 1089, 1225
AcoI YGGCCR 1 cut(s) 768
AcsI RAATTY 2 cut(s) 560, 1251
AcuI CTGAAG 1 cut(s) 1206
AdeI CACNNNGTG 1 cut(s) 648
AfaI GTAC 3 cut(s) 44, 497, 1119
AfiI CCNNNNNNNGG 3 cut(s) 176, 985, 1265
AgsI TTSAA 8 cut(s) 40, 49, 166, 547, 659, 706, 1207, 1217
AjnI CCWGG 3 cut(s) 175, 941, 978
AleI CACNNNNGTG 1 cut(s) 681
Alw21I GWGCWC 2 cut(s) 93, 803
Alw26I GTCTC 1 cut(s) 903
AoxI GGCC 1 cut(s) 768
ApeKI GCWGC 2 cut(s) 566, 616
ApoI RAATTY 2 cut(s) 560, 1251
Asp700I GAANNNNTTC 1 cut(s) 438
AspA2I CCTAGG 1 cut(s) 836
AspS9I GGNCC 1 cut(s) 477
AsuC2I CCSGG 1 cut(s) 1266
AsuHPI GGTGA 5 cut(s) 287, 515, 545, 1168, 1254
AvaII GGWCC 1 cut(s) 477
AvrII CCTAGG 1 cut(s) 836
BalI TGGCCA 1 cut(s) 770
BanI GGYRCC 1 cut(s) 1193
BanII GRGCYC 3 cut(s) 108, 128, 1184
BarI GAAGNNNNNNTAC 2 cut(s) 651, 683
BbsI GAAGAC 2 cut(s) 72, 178
Bbv12I GWGCWC 2 cut(s) 93, 803
BbvI GCAGC 2 cut(s) 553, 603
BccI CCATC 7 cut(s) 50, 136, 272, 578, 812, 816, 1184
BciT130I CCWGG 3 cut(s) 177, 943, 980
BcnI CCSGG 1 cut(s) 1266
BcoDI GTCTC 1 cut(s) 903
BfaI CTAG 4 cut(s) 732, 741, 759, 837
BfmI CTRYAG 2 cut(s) 601, 1289
BisI GCNGC 2 cut(s) 567, 617
BlnI CCTAGG 1 cut(s) 836
BlpI GCTNAGC 3 cut(s) 150, 417, 1166
BlsI GCNGC 2 cut(s) 568, 618
BmcAI AGTACT 2 cut(s) 44, 497
Bme1390I CCNGG 4 cut(s) 177, 943, 980, 1266
Bme18I GGWCC 1 cut(s) 477
BmgT120I GGNCC 1 cut(s) 477
BmiI GGNNCC 7 cut(s) 105, 478, 479, 577, 954, 1125, 1195
BmrFI CCNGG 4 cut(s) 177, 943, 980, 1266
BmsI GCATC 1 cut(s) 844
BpiI GAAGAC 2 cut(s) 72, 178
BplI GAGNNNNNCTC 4 cut(s) 115, 147, 817, 849
BpmI CTGGAG 3 cut(s) 372, 378, 591
Bpu1102I GCTNAGC 3 cut(s) 150, 417, 1166
BpuEI CTTGAG 2 cut(s) 117, 938
BpuMI CCSGG 1 cut(s) 1266
BsaBI GATNNNNATC 1 cut(s) 282
BsaI GGTCTC 1 cut(s) 903
BsaJI CCNNGG 6 cut(s) 144, 155, 836, 941, 942, 1152
Bsc4I CCNNNNNNNGG 3 cut(s) 176, 985, 1265
Bse1I ACTGG 2 cut(s) 395, 747
Bse3DI GCAATG 1 cut(s) 12
Bse8I GATNNNNATC 1 cut(s) 282
BseBI CCWGG 3 cut(s) 177, 943, 980
BseDI CCNNGG 6 cut(s) 144, 155, 836, 941, 942, 1152
BseGI GGATG 6 cut(s) 270, 717, 823, 827, 952, 1108
BseJI GATNNNNATC 1 cut(s) 282
BseLI CCNNNNNNNGG 3 cut(s) 176, 985, 1265
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 2 cut(s) 141, 612
BseNI ACTGG 2 cut(s) 395, 747
BseRI GAGGAG 2 cut(s) 62, 649
BseXI GCAGC 2 cut(s) 553, 603
BseYI CCCAGC 1 cut(s) 992
BsgI GTGCAG 1 cut(s) 893
BshFI GGCC 1 cut(s) 770
BshNI GGYRCC 1 cut(s) 1193
BsiHKAI GWGCWC 2 cut(s) 93, 803
BsiSI CCGG 2 cut(s) 710, 1265
BslFI GGGAC 1 cut(s) 463
BslI CCNNNNNNNGG 3 cut(s) 176, 985, 1265
BsmAI GTCTC 1 cut(s) 903
BsmFI GGGAC 1 cut(s) 463
BsmI GAATGC 1 cut(s) 456
BsnI GGCC 1 cut(s) 770
Bso31I GGTCTC 1 cut(s) 903
Bsp1286I GDGCHC 5 cut(s) 93, 108, 128, 803, 1184
Bsp1407I TGTACA 1 cut(s) 1117
Bsp143I GATC 2 cut(s) 136, 306
Bsp1720I GCTNAGC 3 cut(s) 150, 417, 1166
Bsp19I CCATGG 1 cut(s) 155
BspACI CCGC 2 cut(s) 1089, 1225
BspANI GGCC 1 cut(s) 770
BspCNI CTCAG 2 cut(s) 142, 613
BspHI TCATGA 1 cut(s) 213
BspLI GGNNCC 7 cut(s) 105, 478, 479, 577, 954, 1125, 1195
BspT107I GGYRCC 1 cut(s) 1193
BspTNI GGTCTC 1 cut(s) 903
BsrBI CCGCTC 1 cut(s) 1091
BsrDI GCAATG 1 cut(s) 12
BsrGI TGTACA 1 cut(s) 1117
BsrI ACTGG 2 cut(s) 395, 747
BssECI CCNNGG 6 cut(s) 144, 155, 836, 941, 942, 1152
BssMI GATC 2 cut(s) 136, 306
BssNAI GTATAC 1 cut(s) 843
BssT1I CCWWGG 4 cut(s) 144, 155, 836, 1152
Bst1107I GTATAC 1 cut(s) 843
Bst2UI CCWGG 3 cut(s) 177, 943, 980
Bst4CI ACNGT 1 cut(s) 698
Bst6I CTCTTC 1 cut(s) 194
BstAUI TGTACA 1 cut(s) 1117
BstC8I GCNNGC 2 cut(s) 454, 935
BstDEI CTNAG 5 cut(s) 150, 417, 621, 795, 1166
BstDSI CCRYGG 1 cut(s) 155
BstF5I GGATG 6 cut(s) 270, 717, 823, 827, 952, 1108
BstKTI GATC 2 cut(s) 139, 309
BstMAI GTCTC 1 cut(s) 903
BstMBI GATC 2 cut(s) 136, 306
BstMWI GCNNNNNNNGC 2 cut(s) 112, 776
BstNI CCWGG 3 cut(s) 177, 943, 980
BstSCI CCNGG 4 cut(s) 175, 941, 978, 1264
BstSFI CTRYAG 2 cut(s) 601, 1289
BstV1I GCAGC 2 cut(s) 553, 603
BstV2I GAAGAC 2 cut(s) 72, 178
BstZ17I GTATAC 1 cut(s) 843
BsuRI GGCC 1 cut(s) 770
BtgI CCRYGG 1 cut(s) 155
BtsCI GGATG 6 cut(s) 270, 717, 823, 827, 952, 1108
BtsI GCAGTG 2 cut(s) 187, 887
BtsIMutI CAGTG 5 cut(s) 187, 293, 694, 754, 887
Cac8I GCNNGC 2 cut(s) 454, 935
CciI TCATGA 1 cut(s) 213
Cfr13I GGNCC 1 cut(s) 477
Csp6I GTAC 3 cut(s) 43, 496, 1118
CviAII CATG 9 cut(s) 109, 156, 214, 400, 446, 611, 985, 1074, 1148
CviQI GTAC 3 cut(s) 43, 496, 1118
DdeI CTNAG 5 cut(s) 150, 417, 621, 795, 1166
DpnI GATC 2 cut(s) 138, 308
DpnII GATC 2 cut(s) 136, 306
DraIII CACNNNGTG 1 cut(s) 648
EaeI YGGCCR 1 cut(s) 768
Eam1104I CTCTTC 1 cut(s) 194
EarI CTCTTC 1 cut(s) 194
Eco130I CCWWGG 4 cut(s) 144, 155, 836, 1152
Eco24I GRGCYC 3 cut(s) 108, 128, 1184
Eco31I GGTCTC 1 cut(s) 903
Eco47I GGWCC 1 cut(s) 477
Eco57I CTGAAG 1 cut(s) 1206
EcoO109I RGGNCCY 1 cut(s) 477
EcoRI GAATTC 1 cut(s) 1251
EcoRII CCWGG 3 cut(s) 175, 941, 978
EcoT14I CCWWGG 4 cut(s) 144, 155, 836, 1152
EcoT38I GRGCYC 3 cut(s) 108, 128, 1184
ErhI CCWWGG 4 cut(s) 144, 155, 836, 1152
FaeI CATG 9 cut(s) 112, 159, 217, 403, 449, 614, 988, 1077, 1151
FaqI GGGAC 1 cut(s) 463
FatI CATG 9 cut(s) 108, 155, 213, 399, 445, 610, 984, 1073, 1147
FblI GTMKAC 1 cut(s) 842
Fnu4HI GCNGC 2 cut(s) 567, 617
FokI GGATG 6 cut(s) 277, 704, 830, 834, 959, 1095
FriOI GRGCYC 3 cut(s) 108, 128, 1184
Fsp4HI GCNGC 2 cut(s) 567, 617
FspBI CTAG 4 cut(s) 732, 741, 759, 837
GluI GCNGC 2 cut(s) 567, 617
GsaI CCCAGC 1 cut(s) 996
GsuI CTGGAG 3 cut(s) 372, 378, 591
HaeIII GGCC 1 cut(s) 770
HapII CCGG 2 cut(s) 710, 1265
Hin1II CATG 9 cut(s) 112, 159, 217, 403, 449, 614, 988, 1077, 1151
HincII GTYRAC 2 cut(s) 927, 1069
HindII GTYRAC 2 cut(s) 927, 1069
HinfI GANTC 5 cut(s) 428, 434, 592, 623, 959
HpaI GTTAAC 1 cut(s) 927
HpaII CCGG 2 cut(s) 710, 1265
HphI GGTGA 5 cut(s) 287, 515, 545, 1168, 1254
Hpy166II GTNNAC 5 cut(s) 694, 843, 927, 1069, 1086
Hpy188III TCNNGA 2 cut(s) 134, 214
Hpy8I GTNNAC 5 cut(s) 694, 843, 927, 1069, 1086
HpyAV CCTTC 6 cut(s) 25, 217, 308, 653, 731, 1153
HpyCH4III ACNGT 1 cut(s) 698
HpyCH4V TGCA 3 cut(s) 651, 874, 1064
HpyF10VI GCNNNNNNNGC 2 cut(s) 112, 776
HpyF3I CTNAG 5 cut(s) 150, 417, 621, 795, 1166
Hsp92II CATG 9 cut(s) 112, 159, 217, 403, 449, 614, 988, 1077, 1151
KflI GGGWCCC 1 cut(s) 477
KspAI GTTAAC 1 cut(s) 927
Kzo9I GATC 2 cut(s) 136, 306
LmnI GCTCC 7 cut(s) 103, 364, 397, 636, 952, 1104, 1123
Lsp1109I GCAGC 2 cut(s) 553, 603
LweI GCATC 1 cut(s) 844
MaeI CTAG 4 cut(s) 732, 741, 759, 837
MalI GATC 2 cut(s) 138, 308
MbiI CCGCTC 1 cut(s) 1091
MboI GATC 2 cut(s) 136, 306
MfeI CAATTG 2 cut(s) 679, 1212
MhlI GDGCHC 5 cut(s) 93, 108, 128, 803, 1184
MlsI TGGCCA 1 cut(s) 770
MluCI AATT 6 cut(s) 236, 489, 560, 679, 1212, 1251
MluNI TGGCCA 1 cut(s) 770
MlyI GAGTC 1 cut(s) 632
Mox20I TGGCCA 1 cut(s) 770
MroXI GAANNNNTTC 1 cut(s) 438
MscI TGGCCA 1 cut(s) 770
MseI TTAA 2 cut(s) 369, 926
MslI CAYNNNNRTG 1 cut(s) 681
Msp20I TGGCCA 1 cut(s) 770
MspA1I CMGCKG 1 cut(s) 1283
MspI CCGG 2 cut(s) 710, 1265
MspR9I CCNGG 4 cut(s) 177, 943, 980, 1266
MunI CAATTG 2 cut(s) 679, 1212
Mva1269I GAATGC 1 cut(s) 456
MvaI CCWGG 3 cut(s) 177, 943, 980
MwoI GCNNNNNNNGC 2 cut(s) 112, 776
NciI CCSGG 1 cut(s) 1266
NcoI CCATGG 1 cut(s) 155
NdeII GATC 2 cut(s) 136, 306
NlaIII CATG 9 cut(s) 112, 159, 217, 403, 449, 614, 988, 1077, 1151
NlaIV GGNNCC 7 cut(s) 105, 478, 479, 577, 954, 1125, 1195
OliI CACNNNNGTG 1 cut(s) 681
PagI TCATGA 1 cut(s) 213
PasI CCCWGGG 1 cut(s) 942
PctI GAATGC 1 cut(s) 456
PdmI GAANNNNTTC 1 cut(s) 438
PfeI GAWTC 4 cut(s) 428, 434, 592, 959
PflMI CCANNNNNTGG 1 cut(s) 176
PfoI TCCNGGA 1 cut(s) 978
PkrI GCNGC 2 cut(s) 568, 618
PleI GAGTC 1 cut(s) 631
PpsI GAGTC 1 cut(s) 631
PpuMI RGGWCCY 1 cut(s) 477
Psp5II RGGWCCY 1 cut(s) 477
Psp6I CCWGG 3 cut(s) 175, 941, 978
PspFI CCCAGC 1 cut(s) 992
PspGI CCWGG 3 cut(s) 175, 941, 978
PspN4I GGNNCC 7 cut(s) 105, 478, 479, 577, 954, 1125, 1195
PspPI GGNCC 1 cut(s) 477
PspPPI RGGWCCY 1 cut(s) 477
PvuII CAGCTG 1 cut(s) 1283
RsaI GTAC 3 cut(s) 44, 497, 1119
RsaNI GTAC 3 cut(s) 43, 496, 1118
RseI CAYNNNNRTG 1 cut(s) 681
SaqAI TTAA 2 cut(s) 369, 926
SatI GCNGC 2 cut(s) 567, 617
Sau3AI GATC 2 cut(s) 136, 306
Sau96I GGNCC 1 cut(s) 477
ScaI AGTACT 2 cut(s) 44, 497
SchI GAGTC 1 cut(s) 632
ScrFI CCNGG 4 cut(s) 177, 943, 980, 1266
SduI GDGCHC 5 cut(s) 93, 108, 128, 803, 1184
SfaNI GCATC 1 cut(s) 844
SfcI CTRYAG 2 cut(s) 601, 1289
SinI GGWCC 1 cut(s) 477
SmiMI CAYNNNNRTG 1 cut(s) 681
SmlI CTYRAG 2 cut(s) 132, 917
SmoI CTYRAG 2 cut(s) 132, 917
Sse9I AATT 6 cut(s) 236, 489, 560, 679, 1212, 1251
SsiI CCGC 2 cut(s) 1089, 1225
SspI AATATT 1 cut(s) 543
SspMI CTAG 4 cut(s) 732, 741, 759, 837
StyD4I CCNGG 4 cut(s) 175, 941, 978, 1264
StyI CCWWGG 4 cut(s) 144, 155, 836, 1152
TaaI ACNGT 1 cut(s) 698
TaqI TCGA 1 cut(s) 962
TasI AATT 6 cut(s) 236, 489, 560, 679, 1212, 1251
TatI WGTACW 3 cut(s) 42, 495, 1117
TfiI GAWTC 4 cut(s) 428, 434, 592, 959
Tru1I TTAA 2 cut(s) 369, 926
Tru9I TTAA 2 cut(s) 369, 926
TscAI CASTG 5 cut(s) 187, 293, 701, 754, 887
TseI GCWGC 2 cut(s) 566, 616
TspDTI ATGAA 7 cut(s) 23, 39, 125, 209, 230, 1067, 1097
TspGWI ACGGA 1 cut(s) 235
TspRI CASTG 5 cut(s) 187, 293, 701, 754, 887
Van91I CCANNNNNTGG 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 477
XapI RAATTY 2 cut(s) 560, 1251
XcmI CCANNNNNNNNNTGG 1 cut(s) 811
XmaJI CCTAGG 1 cut(s) 836
XmiI GTMKAC 1 cut(s) 842
XmnI GAANNNNTTC 1 cut(s) 438
XspI CTAG 4 cut(s) 732, 741, 759, 837
ZrmI AGTACT 2 cut(s) 44, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.