Rh1CG256000

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0001294
Physical Location & Seq
Reverse (-)
52056928 .. 52057664
737 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG256000.1

Sequence Viewer

Length: 633 bp
ATGCCCGTGGCCATGGCCATGAAATCGGCAAAGATCAAGGATTCAGATTCCATTCGATACGAGCTCGAGGTGTTTAGGGAGTTGAATAACAAGGGTGGTTCTTGCCCCTTCATCATTGAGCACTATGGTGAAGAGGTGACATGGAGTGACGACGGGGAAGAGGTCTATAACTTGGCCTTGCAAGTTGCATGGGGAAGCCTTGCGAGGCTGATAAAAATTATCGATAAGATCTTGCTTGTGGAAAATGAAGATGATAGTGCCTATACCTTGGTAGCCAAAGTGGGTGACTTGGGGTTGGCCAAGGAGGAGGTTCAAGAAAGGTGGAGAGGCACTCCCATGTTCTTGTCTCCGGAGACTGTCGTGTACAATGAGCAAGAACAAGCCAGTGATATTTGGGCTGTGGGGTGTATTGTTCTTGAAATGCTTACTGGAGAGCGCCCCGGGAATCATTTCTACGATCATGAAAGCGATACTCTCTACGTTGTTGATGGAATGGAGCCTAAAATTCCTGATACGATCTCGAGTCTTGCTAGGGAGTTTCTAGGGTTTTGCCTAGCCAAGGAAACTCCGGAAAGGTGGACAGCTGAAGAGCTTCTATCTCATCCCTTTGTGGCAAATGTTGTTAGGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

210

Amino Acids

23.78

Weight (kDa)

4.51

Isoelectric Point (pI)

38.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 78 - 204 1.4e-19 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 89 - 200 3.9e-09 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 349, 568
AcoI YGGCCR 3 cut(s) 9, 15, 297
AcsI RAATTY 1 cut(s) 504
AcuI CTGAAG 1 cut(s) 606
AfaI GTAC 2 cut(s) 365, 629
AfiI CCNNNNNNNGG 1 cut(s) 559
AgsI TTSAA 3 cut(s) 85, 314, 419
AleI CACNNNNGTG 1 cut(s) 126
AluBI AGCT 3 cut(s) 64, 584, 592
AluI AGCT 3 cut(s) 64, 584, 592
Alw21I GWGCWC 2 cut(s) 66, 123
Alw26I GTCTC 2 cut(s) 347, 351
Ama87I CYCGRG 3 cut(s) 65, 440, 520
Aor13HI TCCGGA 2 cut(s) 349, 568
AoxI GGCC 4 cut(s) 9, 15, 174, 297
ApoI RAATTY 1 cut(s) 504
Asp700I GAANNNNTTC 2 cut(s) 449, 591
AspLEI GCGC 1 cut(s) 438
AsuC2I CCSGG 2 cut(s) 441, 442
AsuHPI GGTGA 3 cut(s) 140, 148, 296
AvaI CYCGRG 3 cut(s) 65, 440, 520
BalI TGGCCA 3 cut(s) 11, 17, 299
BanII GRGCYC 1 cut(s) 66
Bbv12I GWGCWC 2 cut(s) 66, 123
BccI CCATC 1 cut(s) 482
BcnI CCSGG 2 cut(s) 441, 442
BcoDI GTCTC 2 cut(s) 347, 351
BfaI CTAG 3 cut(s) 531, 542, 554
BfoI RGCGCY 1 cut(s) 439
BglII AGATCT 1 cut(s) 228
Bme1390I CCNGG 2 cut(s) 441, 442
BmeT110I CYCGRG 3 cut(s) 65, 440, 520
BmiI GGNNCC 1 cut(s) 498
BmrFI CCNGG 2 cut(s) 441, 442
BplI GAGNNNNNCTC 2 cut(s) 316, 348
BpmI CTGGAG 1 cut(s) 450
BpuMI CCSGG 2 cut(s) 441, 442
Bsa29I ATCGAT 1 cut(s) 222
BsaJI CCNNGG 7 cut(s) 6, 12, 267, 300, 439, 440, 558
BsaWI WCCGGW 2 cut(s) 349, 568
Bsc4I CCNNNNNNNGG 1 cut(s) 559
Bse1I ACTGG 2 cut(s) 384, 433
BseAI TCCGGA 2 cut(s) 349, 568
BseCI ATCGAT 1 cut(s) 222
BseDI CCNNGG 7 cut(s) 6, 12, 267, 300, 439, 440, 558
BseGI GGATG 1 cut(s) 601
BseLI CCNNNNNNNGG 1 cut(s) 559
BseNI ACTGG 2 cut(s) 384, 433
BseRI GAGGAG 1 cut(s) 320
BshFI GGCC 4 cut(s) 11, 17, 176, 299
BshVI ATCGAT 1 cut(s) 222
BsiHKAI GWGCWC 2 cut(s) 66, 123
BsiHKCI CYCGRG 3 cut(s) 65, 440, 520
BsiSI CCGG 3 cut(s) 350, 441, 569
BslI CCNNNNNNNGG 1 cut(s) 559
BsmAI GTCTC 2 cut(s) 347, 351
BsnI GGCC 4 cut(s) 11, 17, 176, 299
BsoBI CYCGRG 3 cut(s) 65, 440, 520
Bsp1286I GDGCHC 2 cut(s) 66, 123
Bsp13I TCCGGA 2 cut(s) 349, 568
Bsp1407I TGTACA 1 cut(s) 363
Bsp143I GATC 4 cut(s) 33, 228, 457, 516
Bsp19I CCATGG 1 cut(s) 12
BspANI GGCC 4 cut(s) 11, 17, 176, 299
BspDI ATCGAT 1 cut(s) 222
BspEI TCCGGA 2 cut(s) 349, 568
BspHI TCATGA 1 cut(s) 460
BspLI GGNNCC 1 cut(s) 498
BspQI GCTCTTC 1 cut(s) 582
BsrGI TGTACA 1 cut(s) 363
BsrI ACTGG 2 cut(s) 384, 433
BssECI CCNNGG 7 cut(s) 6, 12, 267, 300, 439, 440, 558
BssMI GATC 4 cut(s) 33, 228, 457, 516
BssT1I CCWWGG 4 cut(s) 12, 267, 300, 558
Bst4CI ACNGT 1 cut(s) 358
Bst6I CTCTTC 3 cut(s) 126, 153, 582
BstAUI TGTACA 1 cut(s) 363
BstDSI CCRYGG 2 cut(s) 6, 12
BstENI CCTNNNNNAGG 1 cut(s) 557
BstF5I GGATG 1 cut(s) 601
BstH2I RGCGCY 1 cut(s) 439
BstHHI GCGC 1 cut(s) 438
BstKTI GATC 4 cut(s) 36, 231, 460, 519
BstMAI GTCTC 2 cut(s) 347, 351
BstMBI GATC 4 cut(s) 33, 228, 457, 516
BstSCI CCNGG 2 cut(s) 439, 440
BstX2I RGATCY 1 cut(s) 228
BstYI RGATCY 1 cut(s) 228
Bsu15I ATCGAT 1 cut(s) 222
BsuRI GGCC 4 cut(s) 11, 17, 176, 299
BsuTUI ATCGAT 1 cut(s) 222
BtgI CCRYGG 2 cut(s) 6, 12
BtsCI GGATG 1 cut(s) 601
BtsIMutI CAGTG 1 cut(s) 391
CciI TCATGA 1 cut(s) 460
CfoI GCGC 1 cut(s) 438
Cfr9I CCCGGG 1 cut(s) 440
ClaI ATCGAT 1 cut(s) 222
Csp6I GTAC 2 cut(s) 364, 628
CspCI CAANNNNNGTGG 2 cut(s) 302, 337
CviAII CATG 6 cut(s) 13, 19, 141, 189, 337, 461
CviQI GTAC 2 cut(s) 364, 628
DpnI GATC 4 cut(s) 35, 230, 459, 518
DpnII GATC 4 cut(s) 33, 228, 457, 516
EaeI YGGCCR 3 cut(s) 9, 15, 297
Eam1104I CTCTTC 3 cut(s) 126, 153, 582
EarI CTCTTC 3 cut(s) 126, 153, 582
Ecl136II GAGCTC 1 cut(s) 64
Eco130I CCWWGG 4 cut(s) 12, 267, 300, 558
Eco24I GRGCYC 1 cut(s) 66
Eco53kI GAGCTC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 606
Eco88I CYCGRG 3 cut(s) 65, 440, 520
EcoICRI GAGCTC 1 cut(s) 64
EcoNI CCTNNNNNAGG 1 cut(s) 557
EcoT14I CCWWGG 4 cut(s) 12, 267, 300, 558
EcoT38I GRGCYC 1 cut(s) 66
ErhI CCWWGG 4 cut(s) 12, 267, 300, 558
FaeI CATG 6 cut(s) 16, 22, 144, 192, 340, 464
FaiI YATR 9 cut(s) 14, 20, 126, 142, 168, 190, 264, 338, 462
FatI CATG 6 cut(s) 12, 18, 140, 188, 336, 460
FokI GGATG 1 cut(s) 588
FriOI GRGCYC 1 cut(s) 66
FspBI CTAG 3 cut(s) 531, 542, 554
GlaI GCGC 1 cut(s) 437
GsuI CTGGAG 1 cut(s) 450
HaeII RGCGCY 1 cut(s) 439
HaeIII GGCC 4 cut(s) 11, 17, 176, 299
HapII CCGG 3 cut(s) 350, 441, 569
HhaI GCGC 1 cut(s) 438
Hin1II CATG 6 cut(s) 16, 22, 144, 192, 340, 464
Hin6I GCGC 1 cut(s) 436
HinP1I GCGC 1 cut(s) 436
HinfI GANTC 4 cut(s) 41, 47, 445, 523
HpaII CCGG 3 cut(s) 350, 441, 569
HphI GGTGA 3 cut(s) 140, 148, 296
Hpy166II GTNNAC 2 cut(s) 364, 579
Hpy188I TCNGA 1 cut(s) 46
Hpy188III TCNNGA 7 cut(s) 314, 350, 416, 461, 509, 520, 569
Hpy8I GTNNAC 2 cut(s) 364, 579
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 1 cut(s) 118
HpyCH4III ACNGT 1 cut(s) 358
HpyCH4IV ACGT 1 cut(s) 480
HpyCH4V TGCA 2 cut(s) 181, 188
HpySE526I ACGT 1 cut(s) 480
Hsp92II CATG 6 cut(s) 16, 22, 144, 192, 340, 464
HspAI GCGC 1 cut(s) 436
Kpn2I TCCGGA 2 cut(s) 349, 568
Kzo9I GATC 4 cut(s) 33, 228, 457, 516
LguI GCTCTTC 1 cut(s) 582
LmnI GCTCC 1 cut(s) 496
LpnPI CCDG 6 cut(s) 363, 397, 414, 454, 522, 582
MaeI CTAG 3 cut(s) 531, 542, 554
MaeII ACGT 1 cut(s) 480
MaeIII GTNAC 3 cut(s) 136, 146, 284
MalI GATC 4 cut(s) 35, 230, 459, 518
MboI GATC 4 cut(s) 33, 228, 457, 516
MboII GAAGA 4 cut(s) 143, 170, 260, 599
MflI RGATCY 1 cut(s) 228
MhlI GDGCHC 2 cut(s) 66, 123
MlsI TGGCCA 3 cut(s) 11, 17, 299
MluCI AATT 2 cut(s) 216, 504
MluNI TGGCCA 3 cut(s) 11, 17, 299
MlyI GAGTC 1 cut(s) 532
MnlI CCTC 7 cut(s) 61, 127, 154, 198, 298, 301, 320
Mox20I TGGCCA 3 cut(s) 11, 17, 299
MroI TCCGGA 2 cut(s) 349, 568
MroXI GAANNNNTTC 2 cut(s) 449, 591
MscI TGGCCA 3 cut(s) 11, 17, 299
MslI CAYNNNNRTG 3 cut(s) 17, 126, 335
Msp20I TGGCCA 3 cut(s) 11, 17, 299
MspA1I CMGCKG 1 cut(s) 584
MspI CCGG 3 cut(s) 350, 441, 569
MspR9I CCNGG 2 cut(s) 441, 442
NciI CCSGG 2 cut(s) 441, 442
NcoI CCATGG 1 cut(s) 12
NdeII GATC 4 cut(s) 33, 228, 457, 516
NlaIII CATG 6 cut(s) 16, 22, 144, 192, 340, 464
NlaIV GGNNCC 1 cut(s) 498
NmuCI GTSAC 3 cut(s) 136, 146, 284
OliI CACNNNNGTG 1 cut(s) 126
PaeR7I CTCGAG 2 cut(s) 65, 520
PagI TCATGA 1 cut(s) 460
PciSI GCTCTTC 1 cut(s) 582
PdmI GAANNNNTTC 2 cut(s) 449, 591
PfeI GAWTC 3 cut(s) 41, 47, 445
PleI GAGTC 1 cut(s) 531
PpsI GAGTC 1 cut(s) 531
Psp124BI GAGCTC 1 cut(s) 66
PspN4I GGNNCC 1 cut(s) 498
PspXI VCTCGAGB 1 cut(s) 65
PsuI RGATCY 1 cut(s) 228
PvuII CAGCTG 1 cut(s) 584
RsaI GTAC 2 cut(s) 365, 629
RsaNI GTAC 2 cut(s) 364, 628
RseI CAYNNNNRTG 3 cut(s) 17, 126, 335
SacI GAGCTC 1 cut(s) 66
SapI GCTCTTC 1 cut(s) 582
Sau3AI GATC 4 cut(s) 33, 228, 457, 516
SchI GAGTC 1 cut(s) 532
ScrFI CCNGG 2 cut(s) 441, 442
SduI GDGCHC 2 cut(s) 66, 123
Sfr274I CTCGAG 2 cut(s) 65, 520
SlaI CTCGAG 2 cut(s) 65, 520
SmaI CCCGGG 1 cut(s) 442
SmiMI CAYNNNNRTG 3 cut(s) 17, 126, 335
SmlI CTYRAG 2 cut(s) 65, 520
SmoI CTYRAG 2 cut(s) 65, 520
Sse9I AATT 2 cut(s) 216, 504
SspMI CTAG 3 cut(s) 531, 542, 554
SstI GAGCTC 1 cut(s) 66
StyD4I CCNGG 2 cut(s) 439, 440
StyI CCWWGG 4 cut(s) 12, 267, 300, 558
TaaI ACNGT 1 cut(s) 358
TaiI ACGT 1 cut(s) 483
TaqI TCGA 4 cut(s) 55, 66, 222, 521
TasI AATT 2 cut(s) 216, 504
TatI WGTACW 1 cut(s) 363
TfiI GAWTC 3 cut(s) 41, 47, 445
TscAI CASTG 1 cut(s) 391
TseFI GTSAC 3 cut(s) 136, 146, 284
Tsp45I GTSAC 3 cut(s) 136, 146, 284
TspDTI ATGAA 4 cut(s) 35, 100, 261, 477
TspMI CCCGGG 1 cut(s) 440
TspRI CASTG 1 cut(s) 391
XagI CCTNNNNNAGG 1 cut(s) 557
XapI RAATTY 1 cut(s) 504
XhoI CTCGAG 2 cut(s) 65, 520
XmaI CCCGGG 1 cut(s) 440
XmnI GAANNNNTTC 2 cut(s) 449, 591
XspI CTAG 3 cut(s) 531, 542, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.