MD12G1002200.v1.1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001294
Physical Location & Seq
Reverse (-)
379245 .. 380971
1727 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1002200.v1.1.491

Sequence Viewer

Length: 1002 bp
ATGACGAAACGAAAGGCCGAAGAAGAAATACAAGGAGAAAGTCAGAGTCAGAGCATGTGGAATCACGGAGAGCGCTGGTTGAGAGGAGCGTTGATTGGACAAGTTATGGCCGTGAAATCCGCAGAGCTCTCCGCTTCGGAGTCCATTCAGCACGAGGCTGCGGTTCTCTTTGAGATTAAGGGCTGCCCTTTCATTATTGAGCGCTTTGGCGAGGAGACCACAATAACCGAAAAGGGTCACAAGGTCTACAACTTGTTGCTAGAATTTGCTGCAGGAGGAACCCTTGATGGTCTCATCCAACGATCCAATGGTCATGGATTGCCTGAAGCTGAGGTTAAAAGGTATGCAAGGTCGATTCTTGAAGGGGTTAAACATGTTCACAAATGTGGATATGTTCACTGCGATCTAAAGCCCGAGAACATCTTGCTTGTGCCTGTTGCTGCAACTTGTTCTAGTTCTGGTTTTGTAGCCAAGATTGCAGATTTTGGATTGTCAAAGAAAAGAAAGGAGAAATTCAGTGGATGGAGAGGCACTCCTTGGTATCTATCCCCAGAAGCTTTTAAAGATGAGAAGCAAGATCAGTCCTCTGATATATGGTCTCTTGGTTGTATAGTGTTTGAGATGCTAGTGGGCAAGTCGCCCTGGGAATTGAAGCCAGGTTTTGATGATCTTGTCGATTCCTACGACGCGAAGATGTTGGATCATCTGCGTACTTCCAAAATTCCGGCTGGAATCTCATATATGGCCATGGATTTTCTCAAGAGTTGCCTGGCCACGAAACCTGGGAAACGATTAACTGCTGAAGAGCTCTTGTCTCATCCCTTTCTAGCGTGGCCACAACCAACAAAACCAGGTCATGTGAAGGTAAAGTTGGTTAACTCAGCTTTAGGCCACACATACGGCGTGCGTTTCTTAAAACGGAGTGCAGAGTACCACGCAAGCGGTGCCATTGTTCCCAGAATTCAGCCAGCCCCGGGTTTTGGTATACAAGCAGGAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

334

Amino Acids

36.81

Weight (kDa)

8.59

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 33 - 276 2.6e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 37 - 272 7.7e-23 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 944
AccI GTMKAC 2 cut(s) 246, 985
AccII CGCG 1 cut(s) 689
AciI CCGC 4 cut(s) 120, 132, 161, 942
AclWI GGATC 2 cut(s) 297, 708
AcoI YGGCCR 4 cut(s) 108, 744, 771, 833
AcsI RAATTY 4 cut(s) 263, 512, 720, 960
AcuI CTGAAG 2 cut(s) 345, 822
AfaI GTAC 2 cut(s) 712, 932
AfeI AGCGCT 2 cut(s) 74, 203
AfiI CCNNNNNNNGG 2 cut(s) 974, 980
AflIII ACRYGT 1 cut(s) 373
AgsI TTSAA 2 cut(s) 362, 652
AjnI CCWGG 5 cut(s) 641, 655, 768, 781, 850
AjuI GAANNNNNNNTTGG 2 cut(s) 854, 886
AleI CACNNNNGTG 1 cut(s) 384
AluBI AGCT 5 cut(s) 127, 329, 557, 808, 884
AluI AGCT 5 cut(s) 127, 329, 557, 808, 884
Alw21I GWGCWC 2 cut(s) 129, 810
Alw26I GTCTC 4 cut(s) 209, 296, 603, 819
AlwI GGATC 2 cut(s) 297, 708
Ama87I CYCGRG 2 cut(s) 413, 973
Aor51HI AGCGCT 2 cut(s) 74, 203
AoxI GGCC 6 cut(s) 15, 108, 744, 771, 833, 889
ApeKI GCWGC 4 cut(s) 158, 183, 269, 440
ApoI RAATTY 4 cut(s) 263, 512, 720, 960
AspLEI GCGC 2 cut(s) 75, 204
AsuC2I CCSGG 2 cut(s) 974, 975
AvaI CYCGRG 2 cut(s) 413, 973
BalI TGGCCA 3 cut(s) 746, 773, 835
BanI GGYRCC 1 cut(s) 944
BanII GRGCYC 2 cut(s) 129, 810
BarI GAAGNNNNNNTAC 2 cut(s) 12, 44
BauI CACGAG 1 cut(s) 152
Bbv12I GWGCWC 2 cut(s) 129, 810
BbvCI CCTCAGC 1 cut(s) 330
BbvI GCAGC 4 cut(s) 145, 170, 256, 427
BccI CCATC 2 cut(s) 281, 516
BceAI ACGGC 2 cut(s) 95, 916
BciT130I CCWGG 5 cut(s) 643, 657, 770, 783, 852
BcnI CCSGG 2 cut(s) 974, 975
BcoDI GTCTC 4 cut(s) 209, 296, 603, 819
BfaI CTAG 4 cut(s) 260, 453, 626, 827
BfmI CTRYAG 1 cut(s) 270
BfoI RGCGCY 2 cut(s) 76, 205
BisI GCNGC 4 cut(s) 159, 184, 270, 441
BlsI GCNGC 4 cut(s) 160, 185, 271, 442
Bme1390I CCNGG 7 cut(s) 643, 657, 770, 783, 852, 974, 975
BmeT110I CYCGRG 2 cut(s) 413, 973
BmiI GGNNCC 2 cut(s) 280, 946
BmrFI CCNGG 7 cut(s) 643, 657, 770, 783, 852, 974, 975
BmsI GCATC 1 cut(s) 612
BplI GAGNNNNNCTC 2 cut(s) 517, 549
Bpu10I CCTNAGC 1 cut(s) 330
BpuEI CTTGAG 1 cut(s) 743
BpuMI CCSGG 2 cut(s) 974, 975
BsaI GGTCTC 3 cut(s) 209, 296, 603
BsaJI CCNNGG 7 cut(s) 536, 641, 642, 747, 782, 972, 973
Bsc4I CCNNNNNNNGG 2 cut(s) 974, 980
BseBI CCWGG 5 cut(s) 643, 657, 770, 783, 852
BseDI CCNNGG 7 cut(s) 536, 641, 642, 747, 782, 972, 973
BseGI GGATG 3 cut(s) 294, 527, 817
BseLI CCNNNNNNNGG 2 cut(s) 974, 980
BseMII CTCAG 2 cut(s) 321, 894
BseRI GAGGAG 2 cut(s) 99, 227
BseXI GCAGC 4 cut(s) 145, 170, 256, 427
BsgI GTGCAG 1 cut(s) 945
Bsh1236I CGCG 1 cut(s) 689
BshFI GGCC 6 cut(s) 17, 110, 746, 773, 835, 891
BshNI GGYRCC 1 cut(s) 944
BsiHKAI GWGCWC 2 cut(s) 129, 810
BsiHKCI CYCGRG 2 cut(s) 413, 973
BsiSI CCGG 2 cut(s) 725, 974
BslI CCNNNNNNNGG 2 cut(s) 974, 980
BsmAI GTCTC 4 cut(s) 209, 296, 603, 819
BsnI GGCC 6 cut(s) 17, 110, 746, 773, 835, 891
Bso31I GGTCTC 3 cut(s) 209, 296, 603
BsoBI CYCGRG 2 cut(s) 413, 973
Bsp1286I GDGCHC 2 cut(s) 129, 810
Bsp143I GATC 5 cut(s) 302, 403, 577, 667, 700
Bsp19I CCATGG 1 cut(s) 747
BspACI CCGC 4 cut(s) 120, 132, 161, 942
BspANI GGCC 6 cut(s) 17, 110, 746, 773, 835, 891
BspCNI CTCAG 2 cut(s) 322, 893
BspFNI CGCG 1 cut(s) 689
BspLI GGNNCC 2 cut(s) 280, 946
BspMAI CTGCAG 1 cut(s) 274
BspPI GGATC 2 cut(s) 297, 708
BspQI GCTCTTC 1 cut(s) 798
BspT107I GGYRCC 1 cut(s) 944
BspTNI GGTCTC 3 cut(s) 209, 296, 603
BssECI CCNNGG 7 cut(s) 536, 641, 642, 747, 782, 972, 973
BssMI GATC 5 cut(s) 302, 403, 577, 667, 700
BssNAI GTATAC 1 cut(s) 986
BssSI CACGAG 1 cut(s) 152
BssT1I CCWWGG 2 cut(s) 536, 747
Bst1107I GTATAC 1 cut(s) 986
Bst2BI CACGAG 1 cut(s) 152
Bst2UI CCWGG 5 cut(s) 643, 657, 770, 783, 852
Bst6I CTCTTC 1 cut(s) 798
BstAPI GCANNNNNTGC 1 cut(s) 944
BstC8I GCNNGC 3 cut(s) 905, 940, 969
BstDEI CTNAG 2 cut(s) 330, 880
BstDSI CCRYGG 1 cut(s) 747
BstF5I GGATG 3 cut(s) 294, 527, 817
BstFNI CGCG 1 cut(s) 689
BstH2I RGCGCY 2 cut(s) 76, 205
BstHHI GCGC 2 cut(s) 75, 204
BstKTI GATC 5 cut(s) 305, 406, 580, 670, 703
BstMAI GTCTC 4 cut(s) 209, 296, 603, 819
BstMBI GATC 5 cut(s) 302, 403, 577, 667, 700
BstMWI GCNNNNNNNGC 2 cut(s) 476, 944
BstNI CCWGG 5 cut(s) 643, 657, 770, 783, 852
BstNSI RCATGY 2 cut(s) 58, 377
BstSCI CCNGG 7 cut(s) 641, 655, 768, 781, 850, 972, 973
BstSFI CTRYAG 1 cut(s) 270
BstUI CGCG 1 cut(s) 689
BstV1I GCAGC 4 cut(s) 145, 170, 256, 427
BstZ17I GTATAC 1 cut(s) 986
BsuRI GGCC 6 cut(s) 17, 110, 746, 773, 835, 891
BtgI CCRYGG 1 cut(s) 747
BtsCI GGATG 3 cut(s) 294, 527, 817
BtsI GCAGTG 1 cut(s) 397
BtsIMutI CAGTG 2 cut(s) 397, 523
Cac8I GCNNGC 3 cut(s) 905, 940, 969
CfoI GCGC 2 cut(s) 75, 204
Cfr9I CCCGGG 1 cut(s) 973
CseI GACGC 1 cut(s) 695
CsiI ACCWGGT 1 cut(s) 850
Csp6I GTAC 2 cut(s) 711, 931
CviAII CATG 5 cut(s) 55, 314, 374, 748, 857
CviQI GTAC 2 cut(s) 711, 931
DdeI CTNAG 2 cut(s) 330, 880
DpnI GATC 5 cut(s) 304, 405, 579, 669, 702
DpnII GATC 5 cut(s) 302, 403, 577, 667, 700
DraI TTTAAA 1 cut(s) 562
EaeI YGGCCR 4 cut(s) 108, 744, 771, 833
Eam1104I CTCTTC 1 cut(s) 798
EarI CTCTTC 1 cut(s) 798
Ecl136II GAGCTC 2 cut(s) 127, 808
Eco130I CCWWGG 2 cut(s) 536, 747
Eco24I GRGCYC 2 cut(s) 129, 810
Eco31I GGTCTC 3 cut(s) 209, 296, 603
Eco47III AGCGCT 2 cut(s) 74, 203
Eco53kI GAGCTC 2 cut(s) 127, 808
Eco57I CTGAAG 2 cut(s) 345, 822
Eco88I CYCGRG 2 cut(s) 413, 973
EcoICRI GAGCTC 2 cut(s) 127, 808
EcoRI GAATTC 1 cut(s) 960
EcoRII CCWGG 5 cut(s) 641, 655, 768, 781, 850
EcoT14I CCWWGG 2 cut(s) 536, 747
EcoT38I GRGCYC 2 cut(s) 129, 810
ErhI CCWWGG 2 cut(s) 536, 747
FaeI CATG 5 cut(s) 58, 317, 377, 751, 860
FatI CATG 5 cut(s) 54, 313, 373, 747, 856
FblI GTMKAC 2 cut(s) 246, 985
Fnu4HI GCNGC 4 cut(s) 159, 184, 270, 441
FokI GGATG 3 cut(s) 281, 534, 804
FriOI GRGCYC 2 cut(s) 129, 810
Fsp4HI GCNGC 4 cut(s) 159, 184, 270, 441
FspBI CTAG 4 cut(s) 260, 453, 626, 827
GlaI GCGC 2 cut(s) 74, 203
GluI GCNGC 4 cut(s) 159, 184, 270, 441
HaeII RGCGCY 2 cut(s) 76, 205
HaeIII GGCC 6 cut(s) 17, 110, 746, 773, 835, 891
HapII CCGG 2 cut(s) 725, 974
HgaI GACGC 1 cut(s) 695
HhaI GCGC 2 cut(s) 75, 204
Hin1II CATG 5 cut(s) 58, 317, 377, 751, 860
Hin6I GCGC 2 cut(s) 73, 202
HinP1I GCGC 2 cut(s) 73, 202
HincII GTYRAC 1 cut(s) 877
HindII GTYRAC 1 cut(s) 877
HindIII AAGCTT 1 cut(s) 555
HinfI GANTC 6 cut(s) 46, 61, 140, 355, 677, 732
HpaI GTTAAC 1 cut(s) 877
HpaII CCGG 2 cut(s) 725, 974
Hpy166II GTNNAC 5 cut(s) 247, 379, 397, 877, 986
Hpy188I TCNGA 4 cut(s) 45, 51, 139, 589
Hpy188III TCNNGA 2 cut(s) 359, 760
Hpy8I GTNNAC 5 cut(s) 247, 379, 397, 877, 986
Hpy99I CGWCG 1 cut(s) 689
HpyAV CCTTC 2 cut(s) 356, 856
HpyCH4V TGCA 5 cut(s) 272, 347, 443, 479, 926
HpyF10VI GCNNNNNNNGC 2 cut(s) 476, 944
HpyF3I CTNAG 2 cut(s) 330, 880
Hsp92II CATG 5 cut(s) 58, 317, 377, 751, 860
HspAI GCGC 2 cut(s) 73, 202
KspAI GTTAAC 1 cut(s) 877
Kzo9I GATC 5 cut(s) 302, 403, 577, 667, 700
LguI GCTCTTC 1 cut(s) 798
LmnI GCTCC 1 cut(s) 86
Lsp1109I GCAGC 4 cut(s) 145, 170, 256, 427
LweI GCATC 1 cut(s) 612
MabI ACCWGGT 1 cut(s) 850
MaeI CTAG 4 cut(s) 260, 453, 626, 827
MaeIII GTNAC 1 cut(s) 236
MalI GATC 5 cut(s) 304, 405, 579, 669, 702
MboI GATC 5 cut(s) 302, 403, 577, 667, 700
MboII GAAGA 4 cut(s) 32, 35, 703, 815
MhlI GDGCHC 2 cut(s) 129, 810
MlsI TGGCCA 3 cut(s) 746, 773, 835
MluCI AATT 5 cut(s) 263, 512, 647, 720, 960
MluNI TGGCCA 3 cut(s) 746, 773, 835
MlyI GAGTC 2 cut(s) 55, 149
MmeI TCCRAC 2 cut(s) 322, 678
MnlI CCTC 7 cut(s) 77, 148, 205, 269, 325, 521, 595
Mox20I TGGCCA 3 cut(s) 746, 773, 835
MscI TGGCCA 3 cut(s) 746, 773, 835
MseI TTAA 7 cut(s) 177, 336, 369, 561, 794, 876, 914
MslI CAYNNNNRTG 1 cut(s) 384
Msp20I TGGCCA 3 cut(s) 746, 773, 835
MspI CCGG 2 cut(s) 725, 974
MspR9I CCNGG 7 cut(s) 643, 657, 770, 783, 852, 974, 975
MvaI CCWGG 5 cut(s) 643, 657, 770, 783, 852
MvnI CGCG 1 cut(s) 689
MwoI GCNNNNNNNGC 2 cut(s) 476, 944
NciI CCSGG 2 cut(s) 974, 975
NcoI CCATGG 1 cut(s) 747
NdeII GATC 5 cut(s) 302, 403, 577, 667, 700
NlaIII CATG 5 cut(s) 58, 317, 377, 751, 860
NlaIV GGNNCC 2 cut(s) 280, 946
NmuCI GTSAC 1 cut(s) 236
NspI RCATGY 2 cut(s) 58, 377
OliI CACNNNNGTG 1 cut(s) 384
PasI CCCWGGG 1 cut(s) 642
PciI ACATGT 1 cut(s) 373
PciSI GCTCTTC 1 cut(s) 798
PcsI WCGNNNNNNNCGW 1 cut(s) 681
PfeI GAWTC 4 cut(s) 61, 355, 677, 732
PkrI GCNGC 4 cut(s) 160, 185, 271, 442
PleI GAGTC 2 cut(s) 54, 148
PpsI GAGTC 2 cut(s) 54, 148
PscI ACATGT 1 cut(s) 373
Psp124BI GAGCTC 2 cut(s) 129, 810
Psp6I CCWGG 5 cut(s) 641, 655, 768, 781, 850
PspGI CCWGG 5 cut(s) 641, 655, 768, 781, 850
PspN4I GGNNCC 2 cut(s) 280, 946
PstI CTGCAG 1 cut(s) 274
RsaI GTAC 2 cut(s) 712, 932
RsaNI GTAC 2 cut(s) 711, 931
RseI CAYNNNNRTG 1 cut(s) 384
SacI GAGCTC 2 cut(s) 129, 810
SapI GCTCTTC 1 cut(s) 798
SaqAI TTAA 7 cut(s) 177, 336, 369, 561, 794, 876, 914
SatI GCNGC 4 cut(s) 159, 184, 270, 441
Sau3AI GATC 5 cut(s) 302, 403, 577, 667, 700
SchI GAGTC 2 cut(s) 55, 149
ScrFI CCNGG 7 cut(s) 643, 657, 770, 783, 852, 974, 975
SduI GDGCHC 2 cut(s) 129, 810
SexAI ACCWGGT 1 cut(s) 850
SfaNI GCATC 1 cut(s) 612
SfcI CTRYAG 1 cut(s) 270
SmaI CCCGGG 1 cut(s) 975
SmiMI CAYNNNNRTG 1 cut(s) 384
SmlI CTYRAG 1 cut(s) 758
SmoI CTYRAG 1 cut(s) 758
Sse9I AATT 5 cut(s) 263, 512, 647, 720, 960
SsiI CCGC 4 cut(s) 120, 132, 161, 942
SspMI CTAG 4 cut(s) 260, 453, 626, 827
SstI GAGCTC 2 cut(s) 129, 810
StyD4I CCNGG 7 cut(s) 641, 655, 768, 781, 850, 972, 973
StyI CCWWGG 2 cut(s) 536, 747
TaqI TCGA 2 cut(s) 353, 675
TasI AATT 5 cut(s) 263, 512, 647, 720, 960
TfiI GAWTC 4 cut(s) 61, 355, 677, 732
Tru1I TTAA 7 cut(s) 177, 336, 369, 561, 794, 876, 914
Tru9I TTAA 7 cut(s) 177, 336, 369, 561, 794, 876, 914
TscAI CASTG 2 cut(s) 404, 523
TseFI GTSAC 1 cut(s) 236
TseI GCWGC 4 cut(s) 158, 183, 269, 440
Tsp45I GTSAC 1 cut(s) 236
TspDTI ATGAA 1 cut(s) 181
TspGWI ACGGA 2 cut(s) 81, 934
TspMI CCCGGG 1 cut(s) 973
TspRI CASTG 2 cut(s) 404, 523
XapI RAATTY 4 cut(s) 263, 512, 720, 960
XceI RCATGY 2 cut(s) 58, 377
XcmI CCANNNNNNNNNTGG 1 cut(s) 305
XmaI CCCGGG 1 cut(s) 973
XmiI GTMKAC 2 cut(s) 246, 985
XspI CTAG 4 cut(s) 260, 453, 626, 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.