Rh2AG362100

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001294
Physical Location & Seq
Reverse (-)
53677538 .. 53678772
1235 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG362100.1

Sequence Viewer

Length: 306 bp
ATGTACTTGTCTCCTGAAGCTCTAGAAGACAATGTCCAAGAACGACCCAGTGACATATGGGCTGTGGGGTGTATTGTTCTGGAAATGCTTACGGGAGAGTGCCCCCGGAATCATTTCTATGATGGTGAGAGTCGTACTCTCCACATTGTTGACGGAATGGAGCCAAAAATTCCTGATACGATCTCGAGTCAAGCTAAGGATTTTCTACAGCTTTGCCTAGCTACTGTTCCTGAGCAAAGGTGGACAGCTGAAGACCTTCAATCTCATCCCTTCGTGGCAAATCTTGAATTAGATAGTGATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

101

Amino Acids

11.43

Weight (kDa)

4.22

Isoelectric Point (pI)

67.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 2 - 92 1.3e-17 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 2 - 88 2.6e-09 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 168
AcuI CTGAAG 2 cut(s) 36, 270
AfaI GTAC 2 cut(s) 5, 136
AgsI TTSAA 2 cut(s) 260, 287
AluBI AGCT 5 cut(s) 20, 194, 211, 221, 248
AluI AGCT 5 cut(s) 20, 194, 211, 221, 248
Alw26I GTCTC 1 cut(s) 15
Ama87I CYCGRG 1 cut(s) 184
ApoI RAATTY 1 cut(s) 168
Asp700I GAANNNNTTC 2 cut(s) 113, 255
AsuC2I CCSGG 1 cut(s) 106
AsuHPI GGTGA 1 cut(s) 137
AvaI CYCGRG 1 cut(s) 184
BaeGI GKGCMC 1 cut(s) 104
BbsI GAAGAC 2 cut(s) 33, 258
BccI CCATC 1 cut(s) 116
BcnI CCSGG 1 cut(s) 106
BcoDI GTCTC 1 cut(s) 15
BfaI CTAG 2 cut(s) 23, 218
BfmI CTRYAG 1 cut(s) 206
Bme1390I CCNGG 1 cut(s) 106
BmeT110I CYCGRG 1 cut(s) 184
BmiI GGNNCC 1 cut(s) 162
BmrFI CCNGG 1 cut(s) 106
BmrI ACTGGG 1 cut(s) 42
BmuI ACTGGG 1 cut(s) 42
BpiI GAAGAC 2 cut(s) 33, 258
BplI GAGNNNNNCTC 2 cut(s) 121, 153
Bpu10I CCTNAGC 2 cut(s) 195, 231
BpuMI CCSGG 1 cut(s) 106
BsaJI CCNNGG 1 cut(s) 104
Bse1I ACTGG 1 cut(s) 48
BseDI CCNNGG 1 cut(s) 104
BseGI GGATG 1 cut(s) 265
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 48
BseSI GKGCMC 1 cut(s) 104
BsiHKCI CYCGRG 1 cut(s) 184
BsiSI CCGG 1 cut(s) 106
BsmAI GTCTC 1 cut(s) 15
BsoBI CYCGRG 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 104
Bsp143I GATC 1 cut(s) 180
BspCNI CTCAG 1 cut(s) 223
BspLI GGNNCC 1 cut(s) 162
BsrI ACTGG 1 cut(s) 48
BssECI CCNNGG 1 cut(s) 104
BssMI GATC 1 cut(s) 180
Bst4CI ACNGT 1 cut(s) 226
BstDEI CTNAG 2 cut(s) 195, 231
BstF5I GGATG 1 cut(s) 265
BstKTI GATC 1 cut(s) 183
BstMAI GTCTC 1 cut(s) 15
BstMBI GATC 1 cut(s) 180
BstSCI CCNGG 1 cut(s) 104
BstSFI CTRYAG 1 cut(s) 206
BstSLI GKGCMC 1 cut(s) 104
BstV2I GAAGAC 2 cut(s) 33, 258
BtsCI GGATG 1 cut(s) 265
BtsIMutI CAGTG 1 cut(s) 55
Csp6I GTAC 2 cut(s) 4, 135
CviJI RGCY 7 cut(s) 20, 62, 163, 194, 211, 221, 248
CviKI_1 RGCY 7 cut(s) 20, 62, 163, 194, 211, 221, 248
CviQI GTAC 2 cut(s) 4, 135
DdeI CTNAG 2 cut(s) 195, 231
DpnI GATC 1 cut(s) 182
DpnII GATC 1 cut(s) 180
Eco57I CTGAAG 2 cut(s) 36, 270
Eco88I CYCGRG 1 cut(s) 184
FaiI YATR 3 cut(s) 56, 58, 120
FauNDI CATATG 1 cut(s) 56
FokI GGATG 1 cut(s) 252
FspBI CTAG 2 cut(s) 23, 218
HapII CCGG 1 cut(s) 106
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HinfI GANTC 3 cut(s) 109, 130, 187
HpaII CCGG 1 cut(s) 106
HphI GGTGA 1 cut(s) 137
Hpy166II GTNNAC 2 cut(s) 151, 243
Hpy188III TCNNGA 7 cut(s) 14, 23, 80, 173, 184, 230, 284
Hpy8I GTNNAC 2 cut(s) 151, 243
HpyAV CCTTC 2 cut(s) 266, 280
HpyCH4III ACNGT 1 cut(s) 226
HpyF3I CTNAG 2 cut(s) 195, 231
Kzo9I GATC 1 cut(s) 180
LmnI GCTCC 1 cut(s) 160
LpnPI CCDG 6 cut(s) 27, 61, 65, 119, 186, 243
MaeI CTAG 2 cut(s) 23, 218
MaeIII GTNAC 1 cut(s) 50
MalI GATC 1 cut(s) 182
MboI GATC 1 cut(s) 180
MboII GAAGA 2 cut(s) 38, 263
MhlI GDGCHC 1 cut(s) 104
MluCI AATT 3 cut(s) 168, 287, 301
MlyI GAGTC 2 cut(s) 139, 196
MroXI GAANNNNTTC 2 cut(s) 113, 255
MslI CAYNNNNRTG 1 cut(s) 117
MspA1I CMGCKG 1 cut(s) 248
MspI CCGG 1 cut(s) 106
MspR9I CCNGG 1 cut(s) 106
NciI CCSGG 1 cut(s) 106
NdeI CATATG 1 cut(s) 56
NdeII GATC 1 cut(s) 180
NlaIV GGNNCC 1 cut(s) 162
NmuCI GTSAC 1 cut(s) 50
PaeR7I CTCGAG 1 cut(s) 184
PdmI GAANNNNTTC 2 cut(s) 113, 255
PfeI GAWTC 1 cut(s) 109
PflFI GACNNNGTC 1 cut(s) 32
PleI GAGTC 2 cut(s) 138, 195
PpsI GAGTC 2 cut(s) 138, 195
PspN4I GGNNCC 1 cut(s) 162
PsyI GACNNNGTC 1 cut(s) 32
PvuII CAGCTG 1 cut(s) 248
RsaI GTAC 2 cut(s) 5, 136
RsaNI GTAC 2 cut(s) 4, 135
RseI CAYNNNNRTG 1 cut(s) 117
Sau3AI GATC 1 cut(s) 180
SchI GAGTC 2 cut(s) 139, 196
ScrFI CCNGG 1 cut(s) 106
SduI GDGCHC 1 cut(s) 104
SetI ASST 7 cut(s) 22, 196, 213, 223, 242, 250, 258
SfcI CTRYAG 1 cut(s) 206
Sfr274I CTCGAG 1 cut(s) 184
SlaI CTCGAG 1 cut(s) 184
SmiMI CAYNNNNRTG 1 cut(s) 117
SmlI CTYRAG 1 cut(s) 184
SmoI CTYRAG 1 cut(s) 184
Sse9I AATT 3 cut(s) 168, 287, 301
SspMI CTAG 2 cut(s) 23, 218
StyD4I CCNGG 1 cut(s) 104
TaaI ACNGT 1 cut(s) 226
TaqI TCGA 1 cut(s) 185
TasI AATT 3 cut(s) 168, 287, 301
TatI WGTACW 1 cut(s) 3
TfiI GAWTC 1 cut(s) 109
TscAI CASTG 1 cut(s) 55
TseFI GTSAC 1 cut(s) 50
Tsp45I GTSAC 1 cut(s) 50
TspGWI ACGGA 1 cut(s) 168
TspRI CASTG 1 cut(s) 55
Tth111I GACNNNGTC 1 cut(s) 32
XapI RAATTY 1 cut(s) 168
XbaI TCTAGA 1 cut(s) 22
XhoI CTCGAG 1 cut(s) 184
XmnI GAANNNNTTC 2 cut(s) 113, 255
XspI CTAG 2 cut(s) 23, 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.