Rroxscaffold_7G00177180

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001294
Physical Location & Seq
Reverse (-)
16707805 .. 16709382
1578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00177180.1

Sequence Viewer

Length: 861 bp
ATGGAAGCCATTAAGAGCAAGTTCACCGGGCTATGTTTTGCCCGGGCACCACGTCATTTGGCAGCTTGGGACACCAGATTTTCACTATTCCTCCTTTCACCGGATCTGGGGCAAGAGCACAGTGGGCGGCCTAGGACAGCAGCTCGGAAAGATCCGGGCAGTGAAGAGTTTCCTCCTCGTGACCTGGTGGCCCAACGGGTGAACTTGCTTTCTAAGAGGAAGGCAGAAGAGAGCTTGGGTTCAGGGCACAAGTGGATTAGAGGAAACAAGATCGGGGAAGGAGGCTTCGGCTCTGTGTTCGTTGCTTTCTCCAAGAGACCCTACATCCATATCGACCATATGCTTGAGGCCATGGCGGTAAAATCAGCCAAGATGAGGGACTCTGAGTCTATTCAATACGAGCTAGAGGTTTATAGGGACTTGAACAATCATGGTCTTTGCCCCTTCATTATTGAGCATTATGGTGAGGAGGTAACATGGAGCGATGAGGGCGAAGAGGTTTATAACTTGGCGTTGGAAGTTGCATGGGGAAGTCTTGCAAGGCTGATAAAGCCACAAAATATTCTTCTTGTGGAAAATGAAGATGACGACGTCTATAGGTTGGTGGCCAAAGTGGCTGATTTGCGGTTGGCTAAGAAGAAGGATGAGGTTCAAGGAAGGTGGAGAGGCACACCCATGTACTTGTCTCCTGAGACTATACTGTACAATGAACAAGAGGAGCCAAGTGATATTTGGGCCTTGGCATCGGAGAGTGCCCTCGGAACCATTTTTACGATCATGAGAGTGACACCCTCTACGTTGTTGATGGAATGGTGCCTAAAATCCCGGATACAATCTCAAGTCTTGCAAGGGATTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

286

Amino Acids

32.51

Weight (kDa)

5.88

Isoelectric Point (pI)

43.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 183 - 248 9.9e-08 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 504
AatII GACGTC 1 cut(s) 594
AccB1I GGYRCC 2 cut(s) 46, 813
AciI CCGC 3 cut(s) 127, 356, 625
AclWI GGATC 2 cut(s) 111, 146
AcoI YGGCCR 1 cut(s) 606
AcyI GRCGYC 1 cut(s) 591
AfaI GTAC 2 cut(s) 680, 704
AfiI CCNNNNNNNGG 3 cut(s) 100, 107, 375
AgsI TTSAA 3 cut(s) 395, 424, 653
AhdI GACNNNNNGTC 1 cut(s) 385
AjiI CACGTC 1 cut(s) 53
AjnI CCWGG 1 cut(s) 183
AluBI AGCT 4 cut(s) 65, 143, 234, 403
AluI AGCT 4 cut(s) 65, 143, 234, 403
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 3 cut(s) 310, 686, 690
AlwI GGATC 2 cut(s) 111, 146
Ama87I CYCGRG 1 cut(s) 42
AoxI GGCC 5 cut(s) 128, 189, 348, 606, 735
ApeKI GCWGC 2 cut(s) 62, 140
Asp700I GAANNNNTTC 1 cut(s) 168
AspA2I CCTAGG 1 cut(s) 131
AspS9I GGNCC 2 cut(s) 190, 735
AsuC2I CCSGG 5 cut(s) 28, 43, 44, 156, 826
AsuHPI GGTGA 4 cut(s) 16, 90, 211, 476
AvaI CYCGRG 1 cut(s) 42
AvrII CCTAGG 1 cut(s) 131
BaeGI GKGCMC 3 cut(s) 49, 249, 757
BalI TGGCCA 1 cut(s) 608
BanI GGYRCC 2 cut(s) 46, 813
BauI CACGAG 1 cut(s) 177
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 2 cut(s) 74, 152
BccI CCATC 1 cut(s) 799
BciT130I CCWGG 1 cut(s) 185
BciVI GTATCC 1 cut(s) 822
BcnI CCSGG 5 cut(s) 28, 43, 44, 156, 826
BcoDI GTCTC 3 cut(s) 310, 686, 690
BfaI CTAG 3 cut(s) 132, 404, 859
BfmI CTRYAG 1 cut(s) 595
BfuI GTATCC 1 cut(s) 822
BglI GCCNNNNNGGC 1 cut(s) 614
BisI GCNGC 3 cut(s) 63, 128, 141
BlnI CCTAGG 1 cut(s) 131
BlsI GCNGC 3 cut(s) 64, 129, 142
Bme1390I CCNGG 6 cut(s) 28, 43, 44, 156, 185, 826
BmeRI GACNNNNNGTC 1 cut(s) 385
BmeT110I CYCGRG 1 cut(s) 42
BmgBI CACGTC 1 cut(s) 53
BmgT120I GGNCC 2 cut(s) 190, 735
BmiI GGNNCC 4 cut(s) 48, 720, 763, 815
BmrFI CCNGG 6 cut(s) 28, 43, 44, 156, 185, 826
BmsI GCATC 1 cut(s) 752
BpuEI CTTGAG 2 cut(s) 365, 822
BpuMI CCSGG 5 cut(s) 28, 43, 44, 156, 826
BsaHI GRCGYC 1 cut(s) 591
BsaI GGTCTC 1 cut(s) 310
BsaJI CCNNGG 5 cut(s) 42, 131, 351, 738, 757
BsaWI WCCGGW 1 cut(s) 100
BsaXI ACNNNNNCTCC 2 cut(s) 75, 105
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 107, 375
BseBI CCWGG 1 cut(s) 185
BseDI CCNNGG 5 cut(s) 42, 131, 351, 738, 757
BseGI GGATG 2 cut(s) 324, 649
BseLI CCNNNNNNNGG 3 cut(s) 100, 107, 375
BseMII CTCAG 2 cut(s) 375, 681
BseRI GAGGAG 3 cut(s) 165, 482, 731
BseSI GKGCMC 3 cut(s) 49, 249, 757
BseXI GCAGC 2 cut(s) 74, 152
BshFI GGCC 5 cut(s) 130, 191, 350, 608, 737
BshNI GGYRCC 2 cut(s) 46, 813
BsiHKAI GWGCWC 1 cut(s) 120
BsiHKCI CYCGRG 1 cut(s) 42
BsiSI CCGG 5 cut(s) 27, 43, 101, 155, 826
BslFI GGGAC 3 cut(s) 83, 392, 431
BslI CCNNNNNNNGG 3 cut(s) 100, 107, 375
BsmAI GTCTC 3 cut(s) 310, 686, 690
BsmFI GGGAC 3 cut(s) 83, 392, 431
BsnI GGCC 5 cut(s) 130, 191, 350, 608, 737
Bso31I GGTCTC 1 cut(s) 310
BsoBI CYCGRG 1 cut(s) 42
Bsp1286I GDGCHC 4 cut(s) 49, 120, 249, 757
Bsp1407I TGTACA 1 cut(s) 702
Bsp143I GATC 4 cut(s) 103, 151, 270, 774
Bsp19I CCATGG 1 cut(s) 351
BspACI CCGC 3 cut(s) 127, 356, 625
BspANI GGCC 5 cut(s) 130, 191, 350, 608, 737
BspCNI CTCAG 2 cut(s) 376, 682
BspHI TCATGA 1 cut(s) 777
BspLI GGNNCC 4 cut(s) 48, 720, 763, 815
BspPI GGATC 2 cut(s) 111, 146
BspT107I GGYRCC 2 cut(s) 46, 813
BspTNI GGTCTC 1 cut(s) 310
BsrGI TGTACA 1 cut(s) 702
BssECI CCNNGG 5 cut(s) 42, 131, 351, 738, 757
BssMI GATC 4 cut(s) 103, 151, 270, 774
BssNI GRCGYC 1 cut(s) 591
BssSI CACGAG 1 cut(s) 177
BssT1I CCWWGG 3 cut(s) 131, 351, 738
Bst2BI CACGAG 1 cut(s) 177
Bst2UI CCWGG 1 cut(s) 185
Bst4CI ACNGT 2 cut(s) 122, 702
Bst6I CTCTTC 3 cut(s) 159, 222, 489
BstACI GRCGYC 1 cut(s) 591
BstAUI TGTACA 1 cut(s) 702
BstDEI CTNAG 4 cut(s) 213, 384, 633, 690
BstDSI CCRYGG 1 cut(s) 351
BstF5I GGATG 2 cut(s) 324, 649
BstKTI GATC 4 cut(s) 106, 154, 273, 777
BstMAI GTCTC 3 cut(s) 310, 686, 690
BstMBI GATC 4 cut(s) 103, 151, 270, 774
BstMWI GCNNNNNNNGC 4 cut(s) 124, 489, 550, 614
BstNI CCWGG 1 cut(s) 185
BstSCI CCNGG 6 cut(s) 26, 41, 42, 154, 183, 824
BstSFI CTRYAG 1 cut(s) 595
BstSLI GKGCMC 3 cut(s) 49, 249, 757
BstV1I GCAGC 2 cut(s) 74, 152
BstX2I RGATCY 2 cut(s) 103, 151
BstYI RGATCY 2 cut(s) 103, 151
BsuI GTATCC 1 cut(s) 822
BsuRI GGCC 5 cut(s) 130, 191, 350, 608, 737
BtgI CCRYGG 1 cut(s) 351
BtgZI GCGATG 1 cut(s) 498
BtrI CACGTC 1 cut(s) 53
BtsCI GGATG 2 cut(s) 324, 649
BtsI GCAGTG 1 cut(s) 166
BtsIMutI CAGTG 2 cut(s) 127, 166
CciI TCATGA 1 cut(s) 777
Cfr13I GGNCC 2 cut(s) 190, 735
Cfr9I CCCGGG 1 cut(s) 42
CsiI ACCWGGT 1 cut(s) 183
Csp6I GTAC 2 cut(s) 679, 703
CspCI CAANNNNNGTGG 4 cut(s) 39, 74, 641, 676
CviAII CATG 6 cut(s) 352, 431, 477, 525, 676, 778
CviQI GTAC 2 cut(s) 679, 703
DdeI CTNAG 4 cut(s) 213, 384, 633, 690
DpnI GATC 4 cut(s) 105, 153, 272, 776
DpnII GATC 4 cut(s) 103, 151, 270, 774
DriI GACNNNNNGTC 1 cut(s) 385
EaeI YGGCCR 1 cut(s) 606
Eam1104I CTCTTC 3 cut(s) 159, 222, 489
Eam1105I GACNNNNNGTC 1 cut(s) 385
EarI CTCTTC 3 cut(s) 159, 222, 489
Eco130I CCWWGG 3 cut(s) 131, 351, 738
Eco31I GGTCTC 1 cut(s) 310
Eco88I CYCGRG 1 cut(s) 42
EcoRII CCWGG 1 cut(s) 183
EcoT14I CCWWGG 3 cut(s) 131, 351, 738
ErhI CCWWGG 3 cut(s) 131, 351, 738
FaeI CATG 6 cut(s) 355, 434, 480, 528, 679, 781
FaqI GGGAC 3 cut(s) 83, 392, 431
FatI CATG 6 cut(s) 351, 430, 476, 524, 675, 777
FauNDI CATATG 1 cut(s) 339
Fnu4HI GCNGC 3 cut(s) 63, 128, 141
FokI GGATG 2 cut(s) 311, 656
Fsp4HI GCNGC 3 cut(s) 63, 128, 141
FspBI CTAG 3 cut(s) 132, 404, 859
GluI GCNGC 3 cut(s) 63, 128, 141
HaeIII GGCC 5 cut(s) 130, 191, 350, 608, 737
HapII CCGG 5 cut(s) 27, 43, 101, 155, 826
Hin1I GRCGYC 1 cut(s) 591
Hin1II CATG 6 cut(s) 355, 434, 480, 528, 679, 781
HinfI GANTC 2 cut(s) 380, 386
HpaII CCGG 5 cut(s) 27, 43, 101, 155, 826
HphI GGTGA 4 cut(s) 16, 90, 211, 476
Hpy166II GTNNAC 2 cut(s) 24, 202
Hpy188I TCNGA 4 cut(s) 147, 385, 748, 761
Hpy188III TCNNGA 3 cut(s) 179, 689, 778
Hpy8I GTNNAC 2 cut(s) 24, 202
Hpy99I CGWCG 1 cut(s) 593
HpyAV CCTTC 5 cut(s) 214, 272, 454, 634, 651
HpyCH4III ACNGT 2 cut(s) 122, 702
HpyCH4IV ACGT 3 cut(s) 52, 591, 797
HpyCH4V TGCA 3 cut(s) 524, 539, 847
HpyF10VI GCNNNNNNNGC 4 cut(s) 124, 489, 550, 614
HpyF3I CTNAG 4 cut(s) 213, 384, 633, 690
HpySE526I ACGT 3 cut(s) 52, 591, 797
Hsp92I GRCGYC 1 cut(s) 591
Hsp92II CATG 6 cut(s) 355, 434, 480, 528, 679, 781
Kzo9I GATC 4 cut(s) 103, 151, 270, 774
LmnI GCTCC 2 cut(s) 480, 718
Lsp1109I GCAGC 2 cut(s) 74, 152
LweI GCATC 1 cut(s) 752
MabI ACCWGGT 1 cut(s) 183
MaeI CTAG 3 cut(s) 132, 404, 859
MaeII ACGT 3 cut(s) 52, 591, 797
MaeIII GTNAC 3 cut(s) 179, 472, 784
MalI GATC 4 cut(s) 105, 153, 272, 776
MboI GATC 4 cut(s) 103, 151, 270, 774
MboII GAAGA 6 cut(s) 176, 239, 506, 557, 593, 649
MflI RGATCY 2 cut(s) 103, 151
MhlI GDGCHC 4 cut(s) 49, 120, 249, 757
MlsI TGGCCA 1 cut(s) 608
MluNI TGGCCA 1 cut(s) 608
MlyI GAGTC 2 cut(s) 374, 395
MmeI TCCRAC 1 cut(s) 495
Mox20I TGGCCA 1 cut(s) 608
MroXI GAANNNNTTC 1 cut(s) 168
MscI TGGCCA 1 cut(s) 608
MseI TTAA 1 cut(s) 12
MslI CAYNNNNRTG 3 cut(s) 462, 674, 782
Msp20I TGGCCA 1 cut(s) 608
MspI CCGG 5 cut(s) 27, 43, 101, 155, 826
MspR9I CCNGG 6 cut(s) 28, 43, 44, 156, 185, 826
MvaI CCWGG 1 cut(s) 185
MwoI GCNNNNNNNGC 4 cut(s) 124, 489, 550, 614
NciI CCSGG 5 cut(s) 28, 43, 44, 156, 826
NcoI CCATGG 1 cut(s) 351
NdeI CATATG 1 cut(s) 339
NdeII GATC 4 cut(s) 103, 151, 270, 774
NlaIII CATG 6 cut(s) 355, 434, 480, 528, 679, 781
NlaIV GGNNCC 4 cut(s) 48, 720, 763, 815
NmuCI GTSAC 2 cut(s) 179, 784
PagI TCATGA 1 cut(s) 777
PdmI GAANNNNTTC 1 cut(s) 168
PflFI GACNNNGTC 1 cut(s) 590
PfoI TCCNGGA 1 cut(s) 824
PkrI GCNGC 3 cut(s) 64, 129, 142
PleI GAGTC 2 cut(s) 374, 394
PpsI GAGTC 2 cut(s) 374, 394
PsiI TTATAA 1 cut(s) 504
Psp6I CCWGG 1 cut(s) 183
PspGI CCWGG 1 cut(s) 183
PspN4I GGNNCC 4 cut(s) 48, 720, 763, 815
PspPI GGNCC 2 cut(s) 190, 735
PsrI GAACNNNNNNTAC 2 cut(s) 754, 786
PsuI RGATCY 2 cut(s) 103, 151
PsyI GACNNNGTC 1 cut(s) 590
RsaI GTAC 2 cut(s) 680, 704
RsaNI GTAC 2 cut(s) 679, 703
RseI CAYNNNNRTG 3 cut(s) 462, 674, 782
SaqAI TTAA 1 cut(s) 12
SatI GCNGC 3 cut(s) 63, 128, 141
Sau3AI GATC 4 cut(s) 103, 151, 270, 774
Sau96I GGNCC 2 cut(s) 190, 735
SchI GAGTC 2 cut(s) 374, 395
ScrFI CCNGG 6 cut(s) 28, 43, 44, 156, 185, 826
SduI GDGCHC 4 cut(s) 49, 120, 249, 757
SexAI ACCWGGT 1 cut(s) 183
SfaNI GCATC 1 cut(s) 752
SfcI CTRYAG 1 cut(s) 595
SmaI CCCGGG 1 cut(s) 44
SmiMI CAYNNNNRTG 3 cut(s) 462, 674, 782
SmlI CTYRAG 2 cut(s) 344, 837
SmoI CTYRAG 2 cut(s) 344, 837
SrfI GCCCGGGC 1 cut(s) 44
SsiI CCGC 3 cut(s) 127, 356, 625
SspI AATATT 1 cut(s) 562
SspMI CTAG 3 cut(s) 132, 404, 859
StyD4I CCNGG 6 cut(s) 26, 41, 42, 154, 183, 824
StyI CCWWGG 3 cut(s) 131, 351, 738
TaaI ACNGT 2 cut(s) 122, 702
TaiI ACGT 3 cut(s) 55, 594, 800
TaqI TCGA 1 cut(s) 333
TatI WGTACW 2 cut(s) 678, 702
TauI GCSGC 1 cut(s) 130
Tru1I TTAA 1 cut(s) 12
Tru9I TTAA 1 cut(s) 12
TscAI CASTG 2 cut(s) 127, 166
TseFI GTSAC 2 cut(s) 179, 784
TseI GCWGC 2 cut(s) 62, 140
Tsp45I GTSAC 2 cut(s) 179, 784
TspDTI ATGAA 3 cut(s) 436, 594, 723
TspMI CCCGGG 1 cut(s) 42
TspRI CASTG 2 cut(s) 127, 166
Tth111I GACNNNGTC 1 cut(s) 590
XcmI CCANNNNNNNNNTGG 1 cut(s) 729
XmaI CCCGGG 1 cut(s) 42
XmaJI CCTAGG 1 cut(s) 131
XmnI GAANNNNTTC 1 cut(s) 168
XspI CTAG 3 cut(s) 132, 404, 859
ZraI GACGTC 1 cut(s) 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.