Prupe.3G099900_v2.0.a1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001294
Physical Location & Seq
Forward (+)
7664893 .. 7665990
1098 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G099900.1

Sequence Viewer

Length: 1098 bp
ATGAAAAGAAAGGTTGAAGAAGAATTAGATGGGGAAAAGCACAGCATGTGGAGTCATGGAGGGCACTGGCAGAGAGGGGAGTTTATTGGAGAAGGAAGCTTTGGTTCTGTTTTTCTCGCTACTCCCAAGAAACGAAGAAGGGGTGAGTTTAGCCGTATGGTCAATTTGCCTGCGATGATGGCGGTGAAATCGGCAAAGGTGTCTGCTTCGAAGTCTATTGAACATGAGGCAGGGGTTCTGTTTGAGATCAAGGGCTGCCCTTTCGTTATCGAGCGCTTCGGTGAAGAGACCACAACCACAGACAAGGGTGACAAGGTCTACAACTTGTTGTTAGAATTTGCTTCTGGAGGAACCCTTGATGGCCTCATACAAAAATCCAATGGTCTTGGATTGCCTGAATACGATGTTAGAAGATATACAAGGTCAATTCTTGAAGGGATTAGACACATTCACAAGTGTGACTATGTTCACTGCGATTTGAAGCCGGACAACCTTCTGCTTGTGCCTACCACTACCACTACCACTACTTCTACTACCGCTAGGTCTGGTGCTACAACTAGTTTTGTAGCCAAGATTGCAGATTTTGGGTTGGCTAAGAAAACCAAGGACAACTATAGTCGGTGGAGAGGCACTCCTAGGTATCTGTCCCCAGAGGCTTTATTTGATAACAAACAAGATCAGTCCTGTGACATTTGGGCTCTTGGTTGTATTGTGTTTGAGATGCTAACCGGAAACTCCCCCTGGGATTTGAAGCCTGGTTGTGATCTTGACAATTCCGTCGACGTGACGGTGTTTGATCATCTACGCACTTCCAAAATTCCGGCCGAGATCTCAGATGTGGCCAGGGATTTTCTCAAGAGTTGCCTTGCCATGAGGTCCTGTGAAAGATCAACAGCTGAAAGGCTCTTGTCTCATCCATTCGTAGCGCCGCCTCAACCATCAAAAGCGGGTCATGCCAAGTTGAAGGTGGTCAACTCATCTTTGGGATATGCATATGGTGTCTCTTACTTCAAACCGAAAGCAGATTACCGTGCAAGCACTGCCACTGTTCCCAGAATTCATCCCCTACCGGGTTTTGAGATACCAGCTGGACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

366

Amino Acids

40.45

Weight (kDa)

8.77

Isoelectric Point (pI)

44.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 776
AccI GTMKAC 2 cut(s) 318, 780
AciI CCGC 4 cut(s) 182, 537, 929, 947
AcoI YGGCCR 2 cut(s) 822, 840
AcsI RAATTY 3 cut(s) 335, 816, 1056
AfeI AGCGCT 1 cut(s) 275
AfiI CCNNNNNNNGG 1 cut(s) 1070
AgsI TTSAA 7 cut(s) 17, 221, 434, 481, 751, 964, 1012
AhlI ACTAGT 1 cut(s) 557
AjiI CACGTC 1 cut(s) 784
AjnI CCWGG 3 cut(s) 740, 754, 842
AjuI GAANNNNNNNTTGG 2 cut(s) 84, 116
AleI CACNNNNGTG 1 cut(s) 456
AluBI AGCT 3 cut(s) 99, 896, 1088
AluI AGCT 3 cut(s) 99, 896, 1088
Alw26I GTCTC 3 cut(s) 281, 915, 1006
Aor51HI AGCGCT 1 cut(s) 275
AoxI GGCC 3 cut(s) 361, 822, 840
ApeKI GCWGC 1 cut(s) 255
ApoI RAATTY 3 cut(s) 335, 816, 1056
AspA2I CCTAGG 1 cut(s) 635
AspLEI GCGC 2 cut(s) 276, 928
AspS9I GGNCC 1 cut(s) 876
AsuC2I CCSGG 1 cut(s) 1071
AsuHPI GGTGA 4 cut(s) 155, 196, 293, 320
AsuII TTCGAA 1 cut(s) 209
AvaII GGWCC 1 cut(s) 876
AvrII CCTAGG 1 cut(s) 635
BaeGI GKGCMC 1 cut(s) 66
BalI TGGCCA 1 cut(s) 842
BanII GRGCYC 1 cut(s) 700
BbvI GCAGC 1 cut(s) 242
BccI CCATC 4 cut(s) 23, 172, 353, 946
BceAI ACGGC 1 cut(s) 138
BciT130I CCWGG 3 cut(s) 742, 756, 844
BclI TGATCA 1 cut(s) 796
BcnI CCSGG 1 cut(s) 1071
BcoDI GTCTC 3 cut(s) 281, 915, 1006
BcuI ACTAGT 1 cut(s) 557
BfaI CTAG 3 cut(s) 540, 558, 636
BfmI CTRYAG 1 cut(s) 613
BfoI RGCGCY 2 cut(s) 277, 929
BglII AGATCT 1 cut(s) 828
BisI GCNGC 2 cut(s) 256, 929
BlnI CCTAGG 1 cut(s) 635
BlsI GCNGC 2 cut(s) 257, 930
Bme1390I CCNGG 4 cut(s) 742, 756, 844, 1071
Bme18I GGWCC 1 cut(s) 876
BmgBI CACGTC 1 cut(s) 784
BmgT120I GGNCC 1 cut(s) 876
BmiI GGNNCC 1 cut(s) 352
BmrFI CCNGG 4 cut(s) 742, 756, 844, 1071
BmsI GCATC 1 cut(s) 711
BplI GAGNNNNNCTC 2 cut(s) 616, 648
BpmI CTGGAG 1 cut(s) 366
Bpu14I TTCGAA 1 cut(s) 209
BpuEI CTTGAG 1 cut(s) 839
BpuMI CCSGG 1 cut(s) 1071
BsaI GGTCTC 1 cut(s) 281
BsaJI CCNNGG 5 cut(s) 603, 635, 740, 741, 843
BsaWI WCCGGW 1 cut(s) 728
Bsc4I CCNNNNNNNGG 1 cut(s) 1070
Bse1I ACTGG 1 cut(s) 71
BseBI CCWGG 3 cut(s) 742, 756, 844
BseDI CCNNGG 5 cut(s) 603, 635, 740, 741, 843
BseGI GGATG 2 cut(s) 913, 1060
BseLI CCNNNNNNNGG 1 cut(s) 1070
BseMII CTCAG 1 cut(s) 846
BseNI ACTGG 1 cut(s) 71
BseSI GKGCMC 1 cut(s) 66
BseX3I CGGCCG 1 cut(s) 822
BseXI GCAGC 1 cut(s) 242
Bsh1285I CGRYCG 1 cut(s) 825
BshFI GGCC 3 cut(s) 363, 824, 842
BsiEI CGRYCG 1 cut(s) 825
BsiSI CCGG 4 cut(s) 485, 729, 821, 1070
BslFI GGGAC 1 cut(s) 631
BslI CCNNNNNNNGG 1 cut(s) 1070
BsmAI GTCTC 3 cut(s) 281, 915, 1006
BsmFI GGGAC 1 cut(s) 631
BsnI GGCC 3 cut(s) 363, 824, 842
Bso31I GGTCTC 1 cut(s) 281
Bsp119I TTCGAA 1 cut(s) 209
Bsp1286I GDGCHC 2 cut(s) 66, 700
Bsp143I GATC 6 cut(s) 246, 676, 763, 796, 828, 887
BspACI CCGC 4 cut(s) 182, 537, 929, 947
BspANI GGCC 3 cut(s) 363, 824, 842
BspCNI CTCAG 1 cut(s) 845
BspLI GGNNCC 1 cut(s) 352
BspT104I TTCGAA 1 cut(s) 209
BspTNI GGTCTC 1 cut(s) 281
BsrI ACTGG 1 cut(s) 71
BssECI CCNNGG 5 cut(s) 603, 635, 740, 741, 843
BssMI GATC 6 cut(s) 246, 676, 763, 796, 828, 887
BssT1I CCWWGG 2 cut(s) 603, 635
Bst2UI CCWGG 3 cut(s) 742, 756, 844
Bst4CI ACNGT 3 cut(s) 790, 1031, 1048
Bst6I CTCTTC 1 cut(s) 279
BstAPI GCANNNNNTGC 1 cut(s) 1040
BstBI TTCGAA 1 cut(s) 209
BstC8I GCNNGC 2 cut(s) 171, 1036
BstDEI CTNAG 2 cut(s) 594, 832
BstF5I GGATG 2 cut(s) 913, 1060
BstH2I RGCGCY 2 cut(s) 277, 929
BstHHI GCGC 2 cut(s) 276, 928
BstKTI GATC 6 cut(s) 249, 679, 766, 799, 831, 890
BstMAI GTCTC 3 cut(s) 281, 915, 1006
BstMBI GATC 6 cut(s) 246, 676, 763, 796, 828, 887
BstMCI CGRYCG 1 cut(s) 825
BstMWI GCNNNNNNNGC 4 cut(s) 179, 575, 953, 1040
BstNI CCWGG 3 cut(s) 742, 756, 844
BstNSI RCATGY 1 cut(s) 49
BstSCI CCNGG 4 cut(s) 740, 754, 842, 1069
BstSFI CTRYAG 1 cut(s) 613
BstSLI GKGCMC 1 cut(s) 66
BstV1I GCAGC 1 cut(s) 242
BstX2I RGATCY 1 cut(s) 828
BstYI RGATCY 1 cut(s) 828
BstZI CGGCCG 1 cut(s) 822
BsuRI GGCC 3 cut(s) 363, 824, 842
BtgZI GCGATG 1 cut(s) 188
BtrI CACGTC 1 cut(s) 784
BtsCI GGATG 2 cut(s) 913, 1060
BtsI GCAGTG 2 cut(s) 469, 1038
BtsIMutI CAGTG 4 cut(s) 64, 469, 1038, 1044
Cac8I GCNNGC 2 cut(s) 171, 1036
CfoI GCGC 2 cut(s) 276, 928
Cfr13I GGNCC 1 cut(s) 876
CviAII CATG 5 cut(s) 46, 56, 224, 871, 953
DdeI CTNAG 2 cut(s) 594, 832
DpnI GATC 6 cut(s) 248, 678, 765, 798, 830, 889
DpnII GATC 6 cut(s) 246, 676, 763, 796, 828, 887
DrdI GACNNNNNNGTC 1 cut(s) 776
DseDI GACNNNNNNGTC 1 cut(s) 776
EaeI YGGCCR 2 cut(s) 822, 840
EagI CGGCCG 1 cut(s) 822
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
EclXI CGGCCG 1 cut(s) 822
Eco130I CCWWGG 2 cut(s) 603, 635
Eco24I GRGCYC 1 cut(s) 700
Eco31I GGTCTC 1 cut(s) 281
Eco47I GGWCC 1 cut(s) 876
Eco47III AGCGCT 1 cut(s) 275
Eco52I CGGCCG 1 cut(s) 822
EcoO109I RGGNCCY 1 cut(s) 876
EcoRI GAATTC 1 cut(s) 1056
EcoRII CCWGG 3 cut(s) 740, 754, 842
EcoT14I CCWWGG 2 cut(s) 603, 635
EcoT22I ATGCAT 1 cut(s) 994
EcoT38I GRGCYC 1 cut(s) 700
ErhI CCWWGG 2 cut(s) 603, 635
FaeI CATG 5 cut(s) 49, 59, 227, 874, 956
FaqI GGGAC 1 cut(s) 631
FatI CATG 5 cut(s) 45, 55, 223, 870, 952
FauI CCCGC 1 cut(s) 940
FauNDI CATATG 1 cut(s) 994
FbaI TGATCA 1 cut(s) 796
FblI GTMKAC 2 cut(s) 318, 780
Fnu4HI GCNGC 2 cut(s) 256, 929
FokI GGATG 2 cut(s) 900, 1047
FriOI GRGCYC 1 cut(s) 700
Fsp4HI GCNGC 2 cut(s) 256, 929
FspBI CTAG 3 cut(s) 540, 558, 636
GlaI GCGC 2 cut(s) 275, 927
GluI GCNGC 2 cut(s) 256, 929
GsuI CTGGAG 1 cut(s) 366
HaeII RGCGCY 2 cut(s) 277, 929
HaeIII GGCC 3 cut(s) 363, 824, 842
HapII CCGG 4 cut(s) 485, 729, 821, 1070
HhaI GCGC 2 cut(s) 276, 928
Hin1II CATG 5 cut(s) 49, 59, 227, 874, 956
Hin6I GCGC 2 cut(s) 274, 926
HinP1I GCGC 2 cut(s) 274, 926
HincII GTYRAC 2 cut(s) 781, 973
HindII GTYRAC 2 cut(s) 781, 973
HindIII AAGCTT 1 cut(s) 97
HinfI GANTC 1 cut(s) 52
HpaII CCGG 4 cut(s) 485, 729, 821, 1070
HphI GGTGA 4 cut(s) 155, 196, 293, 320
Hpy166II GTNNAC 4 cut(s) 319, 469, 781, 973
Hpy188I TCNGA 1 cut(s) 835
Hpy188III TCNNGA 4 cut(s) 345, 431, 767, 856
Hpy8I GTNNAC 4 cut(s) 319, 469, 781, 973
Hpy99I CGWCG 2 cut(s) 782, 785
HpyAV CCTTC 5 cut(s) 86, 132, 428, 503, 958
HpyCH4III ACNGT 3 cut(s) 790, 1031, 1048
HpyCH4IV ACGT 1 cut(s) 783
HpyCH4V TGCA 3 cut(s) 578, 992, 1034
HpyF10VI GCNNNNNNNGC 4 cut(s) 179, 575, 953, 1040
HpyF3I CTNAG 2 cut(s) 594, 832
HpySE526I ACGT 1 cut(s) 783
Hsp92II CATG 5 cut(s) 49, 59, 227, 874, 956
HspAI GCGC 2 cut(s) 274, 926
Ksp22I TGATCA 1 cut(s) 796
Kzo9I GATC 6 cut(s) 246, 676, 763, 796, 828, 887
Lsp1109I GCAGC 1 cut(s) 242
LweI GCATC 1 cut(s) 711
MaeI CTAG 3 cut(s) 540, 558, 636
MaeII ACGT 1 cut(s) 783
MaeIII GTNAC 4 cut(s) 308, 458, 686, 784
MalI GATC 6 cut(s) 248, 678, 765, 798, 830, 889
MboI GATC 6 cut(s) 246, 676, 763, 796, 828, 887
MboII GAAGA 5 cut(s) 29, 32, 147, 296, 423
MflI RGATCY 1 cut(s) 828
MhlI GDGCHC 2 cut(s) 66, 700
MlsI TGGCCA 1 cut(s) 842
MluCI AATT 7 cut(s) 23, 163, 335, 426, 772, 816, 1056
MluNI TGGCCA 1 cut(s) 842
MlyI GAGTC 1 cut(s) 61
MnlI CCTC 9 cut(s) 53, 68, 220, 341, 374, 620, 646, 867, 942
Mox20I TGGCCA 1 cut(s) 842
Mph1103I ATGCAT 1 cut(s) 994
MscI TGGCCA 1 cut(s) 842
MseI TTAA 1 cut(s) 1096
MslI CAYNNNNRTG 1 cut(s) 456
Msp20I TGGCCA 1 cut(s) 842
MspA1I CMGCKG 2 cut(s) 896, 1088
MspI CCGG 4 cut(s) 485, 729, 821, 1070
MspR9I CCNGG 4 cut(s) 742, 756, 844, 1071
MvaI CCWGG 3 cut(s) 742, 756, 844
MwoI GCNNNNNNNGC 4 cut(s) 179, 575, 953, 1040
NciI CCSGG 1 cut(s) 1071
NdeI CATATG 1 cut(s) 994
NdeII GATC 6 cut(s) 246, 676, 763, 796, 828, 887
NlaIII CATG 5 cut(s) 49, 59, 227, 874, 956
NlaIV GGNNCC 1 cut(s) 352
NmeAIII GCCGAG 1 cut(s) 850
NmuCI GTSAC 4 cut(s) 308, 458, 686, 784
NsiI ATGCAT 1 cut(s) 994
NspI RCATGY 1 cut(s) 49
NspV TTCGAA 1 cut(s) 209
OliI CACNNNNGTG 1 cut(s) 456
PasI CCCWGGG 1 cut(s) 741
PflFI GACNNNGTC 1 cut(s) 314
PkrI GCNGC 2 cut(s) 257, 930
PleI GAGTC 1 cut(s) 60
PpsI GAGTC 1 cut(s) 60
PpuMI RGGWCCY 1 cut(s) 876
Psp5II RGGWCCY 1 cut(s) 876
Psp6I CCWGG 3 cut(s) 740, 754, 842
PspGI CCWGG 3 cut(s) 740, 754, 842
PspN4I GGNNCC 1 cut(s) 352
PspPI GGNCC 1 cut(s) 876
PspPPI RGGWCCY 1 cut(s) 876
PsuI RGATCY 1 cut(s) 828
PsyI GACNNNGTC 1 cut(s) 314
PvuII CAGCTG 2 cut(s) 896, 1088
RseI CAYNNNNRTG 1 cut(s) 456
SalI GTCGAC 1 cut(s) 779
SaqAI TTAA 1 cut(s) 1096
SatI GCNGC 2 cut(s) 256, 929
Sau3AI GATC 6 cut(s) 246, 676, 763, 796, 828, 887
Sau96I GGNCC 1 cut(s) 876
SchI GAGTC 1 cut(s) 61
ScrFI CCNGG 4 cut(s) 742, 756, 844, 1071
SduI GDGCHC 2 cut(s) 66, 700
SfaNI GCATC 1 cut(s) 711
SfcI CTRYAG 1 cut(s) 613
SfuI TTCGAA 1 cut(s) 209
SgrDI CGTCGACG 1 cut(s) 779
SinI GGWCC 1 cut(s) 876
SmiMI CAYNNNNRTG 1 cut(s) 456
SmlI CTYRAG 1 cut(s) 854
SmoI CTYRAG 1 cut(s) 854
SpeI ACTAGT 1 cut(s) 557
Sse9I AATT 7 cut(s) 23, 163, 335, 426, 772, 816, 1056
SsiI CCGC 4 cut(s) 182, 537, 929, 947
SspMI CTAG 3 cut(s) 540, 558, 636
StyD4I CCNGG 4 cut(s) 740, 754, 842, 1069
StyI CCWWGG 2 cut(s) 603, 635
TaaI ACNGT 3 cut(s) 790, 1031, 1048
TaiI ACGT 1 cut(s) 786
TaqI TCGA 3 cut(s) 209, 270, 780
TasI AATT 7 cut(s) 23, 163, 335, 426, 772, 816, 1056
TauI GCSGC 1 cut(s) 931
Tru1I TTAA 1 cut(s) 1096
Tru9I TTAA 1 cut(s) 1096
TscAI CASTG 4 cut(s) 71, 476, 1045, 1051
TseFI GTSAC 4 cut(s) 308, 458, 686, 784
TseI GCWGC 1 cut(s) 255
Tsp45I GTSAC 4 cut(s) 308, 458, 686, 784
TspDTI ATGAA 2 cut(s) 17, 1049
TspGWI ACGGA 1 cut(s) 766
TspRI CASTG 4 cut(s) 71, 476, 1045, 1051
Tth111I GACNNNGTC 1 cut(s) 314
VpaK11BI GGWCC 1 cut(s) 876
XapI RAATTY 3 cut(s) 335, 816, 1056
XceI RCATGY 1 cut(s) 49
XcmI CCANNNNNNNNNTGG 1 cut(s) 964
XmaJI CCTAGG 1 cut(s) 635
XmiI GTMKAC 2 cut(s) 318, 780
XspI CTAG 3 cut(s) 540, 558, 636
Zsp2I ATGCAT 1 cut(s) 994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.