Prupe.3G119300_v2.0.a1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001294
Physical Location & Seq
Reverse (-)
10412379 .. 10414601
2223 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G119300.1

Sequence Viewer

Length: 1143 bp
ATGAAAAGAAAGGTAGATGAAAAATTAGACGAAGAAAACCACAGCATGTGCAGTCATGGAGTGCACTGGTCGAGAGGGAAGTTGATTGGAAAAGGAAGCTATGGTTTTGTTTTTCTTGCTACTCCCAGGAAACCAACAAAGGATGAGCATAGCCGCGGTATGCTCAATTTGCGAGAAATGATGGCGGTGAAATCAGCAAAGGTCTCTGCTTCTGAGTCCATTAAACATGAGTCAGACGTTCTCCTTGAGATCAAGGGTTGCCCTTTCATTATCGAGTGCTTAGGTGAAGAGACCACGGCCACAGACAAGGGTGACATGGTCTACAACTTGTTGTTAGAATTTGCTTCTGGAGGAACCCTAGATGGTCTCATACATAAATCAAAAGGTCATAAATTATCCGAATATGATGTTAGAAGATACACAAGGTCAGTTCTTGAAGGGATTCAACACATTCACAAGTATGACTATGTTCACTGCGATTTGAAGCCGGACAACATTTTGCTTGTGCCTACCACAACAACTAGTTCTGGTGGTACTAGTTTTGTAGCCAAGATTGCAGATTTTGGACTCGCTAAGAAAACCAAAGTAAATTACAGTCGATGGAGAGGCACGCCTAGGTATCTGTCCCCAGAGGCTTTTATTAATAACAAACAAGATCAGTCCTCTGATATTTGGTCTCTAGGTTGTATTGTGTTTGAGATGCTAACCGGCAAGTCGCCCTGGGATTTGAAGCCTGGTTACAATCCAAACAATTTCCCTGACGTGTTGATGTTTGATCATCTACGTACTTGCAAACTTCCGACTGGGATCTCAGATATGGCCAGGGATTTTCTCAAGAGTTGCCTTGCCATGAATAGCAGGGAAAGATTAACAGCGGAAAGTCTCTTGTCTCATCCATTCGTAGCGCAGCCACAATCATCAAAAGAGGGTCATACCAAGATTGCTTTTGTTTGGGCCCTCTTAAAAGTTTCAAATAATGTGCGAACTCACAATGCGAGGAATATGATATGCAAACGAAAGGTACATAGTACAACAATGTGGTCTCTCTGGGGTGAAGAGGGGTACATAAGAATGCAAAGAGATGTTGATGCAGCTGAAGGACTTTGTGGCATAGCGATGTCTGCCTCTTACCCCACTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

381

Amino Acids

42.7

Weight (kDa)

8.89

Isoelectric Point (pI)

37.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 321
AccII CGCG 1 cut(s) 156
AciI CCGC 4 cut(s) 154, 156, 185, 875
AclWI GGATC 1 cut(s) 815
AcoI YGGCCR 2 cut(s) 297, 819
AcsI RAATTY 1 cut(s) 338
AcuI CTGAAG 1 cut(s) 1116
AfaI GTAC 5 cut(s) 535, 787, 1023, 1030, 1064
AflIII ACRYGT 1 cut(s) 762
AgsI TTSAA 5 cut(s) 437, 446, 484, 730, 972
AhlI ACTAGT 2 cut(s) 521, 536
AjiI CACGTC 1 cut(s) 763
AjnI CCWGG 4 cut(s) 125, 719, 733, 821
AluBI AGCT 2 cut(s) 99, 1094
AluI AGCT 2 cut(s) 99, 1094
Alw21I GWGCWC 1 cut(s) 66
Alw26I GTCTC 7 cut(s) 208, 284, 371, 681, 887, 894, 1047
Alw44I GTGCAC 1 cut(s) 62
AlwI GGATC 1 cut(s) 815
AoxI GGCC 3 cut(s) 297, 819, 954
ApaI GGGCCC 1 cut(s) 958
ApaLI GTGCAC 1 cut(s) 62
ApeKI GCWGC 2 cut(s) 907, 1091
ApoI RAATTY 1 cut(s) 338
AseI ATTAAT 1 cut(s) 642
Asp700I GAANNNNTTC 1 cut(s) 441
AspA2I CCTAGG 1 cut(s) 614
AspLEI GCGC 1 cut(s) 907
AspS9I GGNCC 2 cut(s) 954, 955
AsuHPI GGTGA 4 cut(s) 199, 296, 323, 1064
AvrII CCTAGG 1 cut(s) 614
BaeGI GKGCMC 2 cut(s) 66, 958
BalI TGGCCA 1 cut(s) 821
BanII GRGCYC 1 cut(s) 958
BarI GAAGNNNNNNTAC 2 cut(s) 722, 754
Bbv12I GWGCWC 1 cut(s) 66
BbvI GCAGC 2 cut(s) 919, 1103
BccI CCATC 3 cut(s) 175, 356, 594
BceAI ACGGC 1 cut(s) 312
BciT130I CCWGG 4 cut(s) 127, 721, 735, 823
BclI TGATCA 1 cut(s) 775
BcoDI GTCTC 7 cut(s) 208, 284, 371, 681, 887, 894, 1047
BcuI ACTAGT 2 cut(s) 521, 536
BfaI CTAG 5 cut(s) 359, 522, 537, 615, 680
BisI GCNGC 3 cut(s) 154, 908, 1092
BlnI CCTAGG 1 cut(s) 614
BlsI GCNGC 3 cut(s) 155, 909, 1093
Bme1390I CCNGG 4 cut(s) 127, 721, 735, 823
BmgBI CACGTC 1 cut(s) 763
BmgT120I GGNCC 2 cut(s) 954, 955
BmiI GGNNCC 2 cut(s) 355, 956
BmrFI CCNGG 4 cut(s) 127, 721, 735, 823
BmrI ACTGGG 1 cut(s) 813
BmsI GCATC 2 cut(s) 690, 1078
BmuI ACTGGG 1 cut(s) 813
BpmI CTGGAG 1 cut(s) 369
Bpu10I CCTNAGC 1 cut(s) 280
BpuEI CTTGAG 2 cut(s) 266, 818
BsaAI YACGTR 1 cut(s) 785
BsaI GGTCTC 5 cut(s) 208, 284, 371, 681, 1047
BsaJI CCNNGG 7 cut(s) 125, 154, 294, 614, 719, 720, 822
Bse118I RCCGGY 1 cut(s) 707
Bse1I ACTGG 2 cut(s) 71, 808
BseBI CCWGG 4 cut(s) 127, 721, 735, 823
BseDI CCNNGG 7 cut(s) 125, 154, 294, 614, 719, 720, 822
BseGI GGATG 2 cut(s) 148, 892
BseMII CTCAG 2 cut(s) 204, 825
BseNI ACTGG 2 cut(s) 71, 808
BseSI GKGCMC 2 cut(s) 66, 958
BseXI GCAGC 2 cut(s) 919, 1103
BsgI GTGCAG 1 cut(s) 70
Bsh1236I CGCG 1 cut(s) 156
BshFI GGCC 3 cut(s) 299, 821, 956
BsiHKAI GWGCWC 1 cut(s) 66
BsiSI CCGG 2 cut(s) 488, 708
BslFI GGGAC 1 cut(s) 610
BsmAI GTCTC 7 cut(s) 208, 284, 371, 681, 887, 894, 1047
BsmFI GGGAC 1 cut(s) 610
BsmI GAATGC 1 cut(s) 1077
BsnI GGCC 3 cut(s) 299, 821, 956
Bso31I GGTCTC 5 cut(s) 208, 284, 371, 681, 1047
Bsp120I GGGCCC 1 cut(s) 954
Bsp1286I GDGCHC 2 cut(s) 66, 958
Bsp143I GATC 4 cut(s) 249, 655, 775, 807
BspACI CCGC 4 cut(s) 154, 156, 185, 875
BspANI GGCC 3 cut(s) 299, 821, 956
BspCNI CTCAG 2 cut(s) 205, 824
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 2 cut(s) 355, 956
BspPI GGATC 1 cut(s) 815
BspTNI GGTCTC 5 cut(s) 208, 284, 371, 681, 1047
BsrFI RCCGGY 1 cut(s) 707
BsrI ACTGG 2 cut(s) 71, 808
BssAI RCCGGY 1 cut(s) 707
BssECI CCNNGG 7 cut(s) 125, 154, 294, 614, 719, 720, 822
BssMI GATC 4 cut(s) 249, 655, 775, 807
BssT1I CCWWGG 1 cut(s) 614
Bst2UI CCWGG 4 cut(s) 127, 721, 735, 823
Bst4CI ACNGT 1 cut(s) 596
Bst6I CTCTTC 2 cut(s) 282, 1050
BstBAI YACGTR 1 cut(s) 785
BstC8I GCNNGC 1 cut(s) 611
BstDEI CTNAG 4 cut(s) 213, 280, 573, 811
BstDSI CCRYGG 2 cut(s) 154, 294
BstF5I GGATG 2 cut(s) 148, 892
BstFNI CGCG 1 cut(s) 156
BstHHI GCGC 1 cut(s) 907
BstKTI GATC 4 cut(s) 252, 658, 778, 810
BstMAI GTCTC 7 cut(s) 208, 284, 371, 681, 887, 894, 1047
BstMBI GATC 4 cut(s) 249, 655, 775, 807
BstMWI GCNNNNNNNGC 3 cut(s) 169, 554, 1121
BstNI CCWGG 4 cut(s) 127, 721, 735, 823
BstNSI RCATGY 1 cut(s) 49
BstSCI CCNGG 4 cut(s) 125, 719, 733, 821
BstSLI GKGCMC 2 cut(s) 66, 958
BstSNI TACGTA 1 cut(s) 785
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 2 cut(s) 919, 1103
BstX2I RGATCY 1 cut(s) 807
BstYI RGATCY 1 cut(s) 807
BsuRI GGCC 3 cut(s) 299, 821, 956
BtgI CCRYGG 2 cut(s) 154, 294
BtgZI GCGATG 1 cut(s) 1130
BtrI CACGTC 1 cut(s) 763
BtsCI GGATG 2 cut(s) 148, 892
BtsI GCAGTG 2 cut(s) 472, 1134
BtsIMutI CAGTG 3 cut(s) 64, 472, 1134
Cac8I GCNNGC 1 cut(s) 611
CfoI GCGC 1 cut(s) 907
Cfr10I RCCGGY 1 cut(s) 707
Cfr13I GGNCC 2 cut(s) 954, 955
Cfr42I CCGCGG 1 cut(s) 157
Csp6I GTAC 5 cut(s) 534, 786, 1022, 1029, 1063
CviAII CATG 5 cut(s) 46, 56, 227, 316, 850
CviQI GTAC 5 cut(s) 534, 786, 1022, 1029, 1063
DdeI CTNAG 4 cut(s) 213, 280, 573, 811
DpnI GATC 4 cut(s) 251, 657, 777, 809
DpnII GATC 4 cut(s) 249, 655, 775, 807
EaeI YGGCCR 2 cut(s) 297, 819
Eam1104I CTCTTC 2 cut(s) 282, 1050
EarI CTCTTC 2 cut(s) 282, 1050
Eco105I TACGTA 1 cut(s) 785
Eco130I CCWWGG 1 cut(s) 614
Eco24I GRGCYC 1 cut(s) 958
Eco31I GGTCTC 5 cut(s) 208, 284, 371, 681, 1047
Eco57I CTGAAG 1 cut(s) 1116
EcoO109I RGGNCCY 1 cut(s) 955
EcoRII CCWGG 4 cut(s) 125, 719, 733, 821
EcoT14I CCWWGG 1 cut(s) 614
EcoT38I GRGCYC 1 cut(s) 958
ErhI CCWWGG 1 cut(s) 614
FaeI CATG 5 cut(s) 49, 59, 230, 319, 853
FaqI GGGAC 1 cut(s) 610
FatI CATG 5 cut(s) 45, 55, 226, 315, 849
FbaI TGATCA 1 cut(s) 775
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 3 cut(s) 154, 908, 1092
FokI GGATG 2 cut(s) 155, 879
FriOI GRGCYC 1 cut(s) 958
Fsp4HI GCNGC 3 cut(s) 154, 908, 1092
FspBI CTAG 5 cut(s) 359, 522, 537, 615, 680
GlaI GCGC 1 cut(s) 906
GluI GCNGC 3 cut(s) 154, 908, 1092
GsuI CTGGAG 1 cut(s) 369
HaeIII GGCC 3 cut(s) 299, 821, 956
HapII CCGG 2 cut(s) 488, 708
HhaI GCGC 1 cut(s) 907
Hin1II CATG 5 cut(s) 49, 59, 230, 319, 853
Hin6I GCGC 1 cut(s) 905
HinP1I GCGC 1 cut(s) 905
HinfI GANTC 4 cut(s) 215, 230, 442, 567
HpaII CCGG 2 cut(s) 488, 708
HphI GGTGA 4 cut(s) 199, 296, 323, 1064
Hpy166II GTNNAC 3 cut(s) 64, 322, 472
Hpy188I TCNGA 6 cut(s) 214, 235, 400, 667, 801, 814
Hpy188III TCNNGA 4 cut(s) 72, 348, 434, 835
Hpy8I GTNNAC 3 cut(s) 64, 322, 472
HpyAV CCTTC 2 cut(s) 431, 1091
HpyCH4III ACNGT 1 cut(s) 596
HpyCH4IV ACGT 3 cut(s) 237, 762, 784
HpyCH4V TGCA 8 cut(s) 51, 64, 557, 792, 1011, 1075, 1091, 1139
HpyF10VI GCNNNNNNNGC 3 cut(s) 169, 554, 1121
HpyF3I CTNAG 4 cut(s) 213, 280, 573, 811
HpySE526I ACGT 3 cut(s) 237, 762, 784
Hsp92II CATG 5 cut(s) 49, 59, 230, 319, 853
HspAI GCGC 1 cut(s) 905
Ksp22I TGATCA 1 cut(s) 775
KspI CCGCGG 1 cut(s) 157
Kzo9I GATC 4 cut(s) 249, 655, 775, 807
Lsp1109I GCAGC 2 cut(s) 919, 1103
LweI GCATC 2 cut(s) 690, 1078
MaeI CTAG 5 cut(s) 359, 522, 537, 615, 680
MaeII ACGT 3 cut(s) 237, 762, 784
MaeIII GTNAC 2 cut(s) 311, 737
MalI GATC 4 cut(s) 251, 657, 777, 809
MboI GATC 4 cut(s) 249, 655, 775, 807
MboII GAAGA 4 cut(s) 44, 299, 426, 1067
MflI RGATCY 1 cut(s) 807
MhlI GDGCHC 2 cut(s) 66, 958
MlsI TGGCCA 1 cut(s) 821
MluCI AATT 6 cut(s) 23, 166, 338, 392, 589, 751
MluNI TGGCCA 1 cut(s) 821
MlyI GAGTC 3 cut(s) 224, 239, 561
MmeI TCCRAC 1 cut(s) 824
Mox20I TGGCCA 1 cut(s) 821
MroXI GAANNNNTTC 1 cut(s) 441
MscI TGGCCA 1 cut(s) 821
MseI TTAA 4 cut(s) 222, 642, 869, 962
MslI CAYNNNNRTG 3 cut(s) 459, 1070, 1115
Msp20I TGGCCA 1 cut(s) 821
MspA1I CMGCKG 3 cut(s) 156, 875, 1094
MspI CCGG 2 cut(s) 488, 708
MspR9I CCNGG 4 cut(s) 127, 721, 735, 823
Mva1269I GAATGC 1 cut(s) 1077
MvaI CCWGG 4 cut(s) 127, 721, 735, 823
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 3 cut(s) 169, 554, 1121
NdeII GATC 4 cut(s) 249, 655, 775, 807
NlaIII CATG 5 cut(s) 49, 59, 230, 319, 853
NlaIV GGNNCC 2 cut(s) 355, 956
NmuCI GTSAC 1 cut(s) 311
NspI RCATGY 1 cut(s) 49
PasI CCCWGGG 1 cut(s) 720
PctI GAATGC 1 cut(s) 1077
PdmI GAANNNNTTC 1 cut(s) 441
PfeI GAWTC 1 cut(s) 442
PflFI GACNNNGTC 1 cut(s) 317
PkrI GCNGC 3 cut(s) 155, 909, 1093
PleI GAGTC 3 cut(s) 223, 238, 561
PpsI GAGTC 3 cut(s) 223, 238, 561
Ppu21I YACGTR 1 cut(s) 785
PshBI ATTAAT 1 cut(s) 642
Psp6I CCWGG 4 cut(s) 125, 719, 733, 821
PspGI CCWGG 4 cut(s) 125, 719, 733, 821
PspN4I GGNNCC 2 cut(s) 355, 956
PspOMI GGGCCC 1 cut(s) 954
PspPI GGNCC 2 cut(s) 954, 955
PsuI RGATCY 1 cut(s) 807
PsyI GACNNNGTC 1 cut(s) 317
PvuII CAGCTG 1 cut(s) 1094
RsaI GTAC 5 cut(s) 535, 787, 1023, 1030, 1064
RsaNI GTAC 5 cut(s) 534, 786, 1022, 1029, 1063
RseI CAYNNNNRTG 3 cut(s) 459, 1070, 1115
SacII CCGCGG 1 cut(s) 157
SaqAI TTAA 4 cut(s) 222, 642, 869, 962
SatI GCNGC 3 cut(s) 154, 908, 1092
Sau3AI GATC 4 cut(s) 249, 655, 775, 807
Sau96I GGNCC 2 cut(s) 954, 955
SchI GAGTC 3 cut(s) 224, 239, 561
ScrFI CCNGG 4 cut(s) 127, 721, 735, 823
SduI GDGCHC 2 cut(s) 66, 958
SfaNI GCATC 2 cut(s) 690, 1078
Sfr303I CCGCGG 1 cut(s) 157
SgrBI CCGCGG 1 cut(s) 157
SmiMI CAYNNNNRTG 3 cut(s) 459, 1070, 1115
SmlI CTYRAG 2 cut(s) 245, 833
SmoI CTYRAG 2 cut(s) 245, 833
SnaBI TACGTA 1 cut(s) 785
SpeI ACTAGT 2 cut(s) 521, 536
Sse9I AATT 6 cut(s) 23, 166, 338, 392, 589, 751
SsiI CCGC 4 cut(s) 154, 156, 185, 875
SspMI CTAG 5 cut(s) 359, 522, 537, 615, 680
StyD4I CCNGG 4 cut(s) 125, 719, 733, 821
StyI CCWWGG 1 cut(s) 614
TaaI ACNGT 1 cut(s) 596
TaiI ACGT 3 cut(s) 240, 765, 787
TaqI TCGA 3 cut(s) 71, 273, 598
TasI AATT 6 cut(s) 23, 166, 338, 392, 589, 751
TatI WGTACW 1 cut(s) 1028
TauI GCSGC 1 cut(s) 156
TfiI GAWTC 1 cut(s) 442
Tru1I TTAA 4 cut(s) 222, 642, 869, 962
Tru9I TTAA 4 cut(s) 222, 642, 869, 962
TscAI CASTG 3 cut(s) 71, 479, 1141
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 2 cut(s) 907, 1091
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 4 cut(s) 17, 33, 256, 866
TspRI CASTG 3 cut(s) 71, 479, 1141
Tth111I GACNNNGTC 1 cut(s) 317
VneI GTGCAC 1 cut(s) 62
VspI ATTAAT 1 cut(s) 642
XapI RAATTY 1 cut(s) 338
XceI RCATGY 1 cut(s) 49
XmaJI CCTAGG 1 cut(s) 614
XmiI GTMKAC 1 cut(s) 321
XmnI GAANNNNTTC 1 cut(s) 441
XspI CTAG 5 cut(s) 359, 522, 537, 615, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.