pycom09g14650

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0001294
Physical Location & Seq
Forward (+)
14392052 .. 14392852
801 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g14650.1

Sequence Viewer

Length: 720 bp
ATGGTGAAAACAAAAGCGGAAGAAGAAACTCAGAGCATGTGCAATCATGGAGAGCGCTGGTTGAGAGGAGCCTTGATTGGAAAAGGAGGATTTGGTTCGGTTTTTCTTGCTACTCCAGATAAACGTAAAAGGGGTCTCTTGACTGGCAATTTTCCTGCATTGATGGCCGTGAAATTGGCAGAGCCCTCTGCTTCAGAGCCCATTCAGCATGAGGCAGAGGTTCTCTTTGAGATTAAGGGCTGCTCTTTTATCAGCGAGTGGTTTGGCGAAGAGACCGCAACCACCGAAAAGGGCGACAAGGTCCACAACTTGCTATTAGAATATAATGGATTGTCTAAGAGAAGCAAGGAGAAATACAGTGGATGGAGAGGCACTCCTAGGTATCTATCCATTGAATCTCTTGGTTGTATAGTGTTTGAGATGCTAGCAGGCAAGTTGCCCTGGGAGTTGAAGCCAGGTTGTAGTGATCATGTCGATATACTCGATGTGACAATGTTGGATCATCTGCGTACTTCAAGAATTGCTGCTGAAATCTCATATGTGGTCAGGGATTTTCTAGAGAGTTCTCCTGTCTCATCTGTAGCGCAGCTGCACCCAAAAAAACCAGGTCATGTCACGGTAAAGGTGGTTAACTCAGCCTTAGATCAAGCATATGGCGTCTGTTGCTTCAAACCGAGTGCAAAATACCATGCAAGCACTGTGATTGTTCCTAGAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

240

Amino Acids

26.41

Weight (kDa)

8.16

Isoelectric Point (pI)

32.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 17, 276
AclWI GGATC 1 cut(s) 507
AcoI YGGCCR 1 cut(s) 165
AcsI RAATTY 1 cut(s) 714
AcuI CTGAAG 1 cut(s) 177
AcyI GRCGYC 1 cut(s) 657
AfaI GTAC 1 cut(s) 511
AfeI AGCGCT 1 cut(s) 56
AgsI TTSAA 4 cut(s) 395, 451, 516, 670
AjnI CCWGG 3 cut(s) 440, 454, 604
AluBI AGCT 1 cut(s) 589
AluI AGCT 1 cut(s) 589
Alw26I GTCTC 3 cut(s) 140, 266, 577
AlwI GGATC 1 cut(s) 507
Aor51HI AGCGCT 1 cut(s) 56
AoxI GGCC 1 cut(s) 165
ApeKI GCWGC 4 cut(s) 240, 524, 586, 589
ApoI RAATTY 1 cut(s) 714
AspA2I CCTAGG 1 cut(s) 377
AspLEI GCGC 2 cut(s) 57, 586
AspS9I GGNCC 1 cut(s) 301
AsuHPI GGTGA 1 cut(s) 16
AsuNHI GCTAGC 1 cut(s) 424
AvaII GGWCC 1 cut(s) 301
AvrII CCTAGG 1 cut(s) 377
BanII GRGCYC 2 cut(s) 186, 201
BbvI GCAGC 4 cut(s) 227, 511, 576, 598
BccI CCATC 2 cut(s) 157, 357
BceAI ACGGC 1 cut(s) 152
BciT130I CCWGG 3 cut(s) 442, 456, 606
BclI TGATCA 1 cut(s) 466
BcoDI GTCTC 3 cut(s) 140, 266, 577
BfaI CTAG 4 cut(s) 378, 425, 557, 711
BfmI CTRYAG 1 cut(s) 579
BfoI RGCGCY 1 cut(s) 58
BisI GCNGC 4 cut(s) 241, 525, 587, 590
BlnI CCTAGG 1 cut(s) 377
BlsI GCNGC 4 cut(s) 242, 526, 588, 591
Bme1390I CCNGG 3 cut(s) 442, 456, 606
Bme18I GGWCC 1 cut(s) 301
BmgT120I GGNCC 1 cut(s) 301
BmiI GGNNCC 1 cut(s) 70
BmrFI CCNGG 3 cut(s) 442, 456, 606
BmsI GCATC 1 cut(s) 411
BmtI GCTAGC 1 cut(s) 428
BplI GAGNNNNNCTC 2 cut(s) 358, 390
BpmI CTGGAG 1 cut(s) 99
BsaHI GRCGYC 1 cut(s) 657
BsaI GGTCTC 2 cut(s) 140, 266
BsaJI CCNNGG 3 cut(s) 377, 440, 441
Bse1I ACTGG 1 cut(s) 148
BseBI CCWGG 3 cut(s) 442, 456, 606
BseDI CCNNGG 3 cut(s) 377, 440, 441
BseGI GGATG 1 cut(s) 368
BseMII CTCAG 2 cut(s) 44, 648
BseNI ACTGG 1 cut(s) 148
BseRI GAGGAG 1 cut(s) 81
BseXI GCAGC 4 cut(s) 227, 511, 576, 598
BsgI GTGCAG 1 cut(s) 575
BshFI GGCC 1 cut(s) 167
BsmAI GTCTC 3 cut(s) 140, 266, 577
BsnI GGCC 1 cut(s) 167
Bso31I GGTCTC 2 cut(s) 140, 266
Bsp1286I GDGCHC 2 cut(s) 186, 201
Bsp143I GATC 3 cut(s) 466, 499, 643
BspACI CCGC 2 cut(s) 17, 276
BspANI GGCC 1 cut(s) 167
BspCNI CTCAG 2 cut(s) 43, 647
BspLI GGNNCC 1 cut(s) 70
BspOI GCTAGC 1 cut(s) 428
BspPI GGATC 1 cut(s) 507
BspTNI GGTCTC 2 cut(s) 140, 266
BsrI ACTGG 1 cut(s) 148
BssECI CCNNGG 3 cut(s) 377, 440, 441
BssMI GATC 3 cut(s) 466, 499, 643
BssNI GRCGYC 1 cut(s) 657
BssT1I CCWWGG 1 cut(s) 377
Bst2UI CCWGG 3 cut(s) 442, 456, 606
Bst4CI ACNGT 3 cut(s) 359, 619, 700
Bst6I CTCTTC 1 cut(s) 264
BstACI GRCGYC 1 cut(s) 657
BstC8I GCNNGC 3 cut(s) 426, 430, 694
BstDEI CTNAG 4 cut(s) 30, 336, 634, 640
BstF5I GGATG 1 cut(s) 368
BstH2I RGCGCY 1 cut(s) 58
BstHHI GCGC 2 cut(s) 57, 586
BstKTI GATC 3 cut(s) 469, 502, 646
BstMAI GTCTC 3 cut(s) 140, 266, 577
BstMBI GATC 3 cut(s) 466, 499, 643
BstMWI GCNNNNNNNGC 3 cut(s) 164, 205, 663
BstNI CCWGG 3 cut(s) 442, 456, 606
BstNSI RCATGY 1 cut(s) 40
BstSCI CCNGG 3 cut(s) 440, 454, 604
BstSFI CTRYAG 1 cut(s) 579
BstV1I GCAGC 4 cut(s) 227, 511, 576, 598
BsuRI GGCC 1 cut(s) 167
BtsCI GGATG 1 cut(s) 368
BtsIMutI CAGTG 2 cut(s) 364, 696
Cac8I GCNNGC 3 cut(s) 426, 430, 694
CfoI GCGC 2 cut(s) 57, 586
Cfr13I GGNCC 1 cut(s) 301
CseI GACGC 1 cut(s) 646
CsiI ACCWGGT 1 cut(s) 604
Csp6I GTAC 1 cut(s) 510
CviAII CATG 6 cut(s) 37, 47, 209, 470, 611, 689
CviJI RGCY 8 cut(s) 71, 167, 184, 199, 240, 454, 589, 638
CviKI_1 RGCY 8 cut(s) 71, 167, 184, 199, 240, 454, 589, 638
CviQI GTAC 1 cut(s) 510
DdeI CTNAG 4 cut(s) 30, 336, 634, 640
DpnI GATC 3 cut(s) 468, 501, 645
DpnII GATC 3 cut(s) 466, 499, 643
EaeI YGGCCR 1 cut(s) 165
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
Eco130I CCWWGG 1 cut(s) 377
Eco24I GRGCYC 2 cut(s) 186, 201
Eco31I GGTCTC 2 cut(s) 140, 266
Eco47I GGWCC 1 cut(s) 301
Eco47III AGCGCT 1 cut(s) 56
Eco57I CTGAAG 1 cut(s) 177
EcoRII CCWGG 3 cut(s) 440, 454, 604
EcoT14I CCWWGG 1 cut(s) 377
EcoT38I GRGCYC 2 cut(s) 186, 201
ErhI CCWWGG 1 cut(s) 377
FaeI CATG 6 cut(s) 40, 50, 212, 473, 614, 692
FatI CATG 6 cut(s) 36, 46, 208, 469, 610, 688
FauNDI CATATG 2 cut(s) 538, 652
FbaI TGATCA 1 cut(s) 466
Fnu4HI GCNGC 4 cut(s) 241, 525, 587, 590
FokI GGATG 1 cut(s) 375
FriOI GRGCYC 2 cut(s) 186, 201
Fsp4HI GCNGC 4 cut(s) 241, 525, 587, 590
FspBI CTAG 4 cut(s) 378, 425, 557, 711
GlaI GCGC 2 cut(s) 56, 585
GluI GCNGC 4 cut(s) 241, 525, 587, 590
GsuI CTGGAG 1 cut(s) 99
HaeII RGCGCY 1 cut(s) 58
HaeIII GGCC 1 cut(s) 167
HgaI GACGC 1 cut(s) 646
HhaI GCGC 2 cut(s) 57, 586
Hin1I GRCGYC 1 cut(s) 657
Hin1II CATG 6 cut(s) 40, 50, 212, 473, 614, 692
Hin6I GCGC 2 cut(s) 55, 584
HinP1I GCGC 2 cut(s) 55, 584
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HinfI GANTC 1 cut(s) 395
HpaI GTTAAC 1 cut(s) 631
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 304, 631
Hpy188I TCNGA 2 cut(s) 33, 196
Hpy188III TCNNGA 4 cut(s) 116, 139, 516, 557
Hpy8I GTNNAC 2 cut(s) 304, 631
HpyCH4III ACNGT 3 cut(s) 359, 619, 700
HpyCH4IV ACGT 1 cut(s) 124
HpyCH4V TGCA 5 cut(s) 42, 158, 592, 680, 692
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 205, 663
HpyF3I CTNAG 4 cut(s) 30, 336, 634, 640
HpySE526I ACGT 1 cut(s) 124
Hsp92I GRCGYC 1 cut(s) 657
Hsp92II CATG 6 cut(s) 40, 50, 212, 473, 614, 692
HspAI GCGC 2 cut(s) 55, 584
Ksp22I TGATCA 1 cut(s) 466
KspAI GTTAAC 1 cut(s) 631
Kzo9I GATC 3 cut(s) 466, 499, 643
LmnI GCTCC 1 cut(s) 68
Lsp1109I GCAGC 4 cut(s) 227, 511, 576, 598
LweI GCATC 1 cut(s) 411
MabI ACCWGGT 1 cut(s) 604
MaeI CTAG 4 cut(s) 378, 425, 557, 711
MaeII ACGT 1 cut(s) 124
MaeIII GTNAC 2 cut(s) 487, 613
MalI GATC 3 cut(s) 468, 501, 645
MboI GATC 3 cut(s) 466, 499, 643
MboII GAAGA 3 cut(s) 32, 35, 281
MhlI GDGCHC 2 cut(s) 186, 201
MluCI AATT 4 cut(s) 148, 173, 519, 714
MmeI TCCRAC 1 cut(s) 477
MnlI CCTC 6 cut(s) 59, 80, 196, 205, 211, 362
MseI TTAA 2 cut(s) 234, 630
MspA1I CMGCKG 1 cut(s) 589
MspR9I CCNGG 3 cut(s) 442, 456, 606
MvaI CCWGG 3 cut(s) 442, 456, 606
MwoI GCNNNNNNNGC 3 cut(s) 164, 205, 663
NdeI CATATG 2 cut(s) 538, 652
NdeII GATC 3 cut(s) 466, 499, 643
NheI GCTAGC 1 cut(s) 424
NlaIII CATG 6 cut(s) 40, 50, 212, 473, 614, 692
NlaIV GGNNCC 1 cut(s) 70
NmuCI GTSAC 2 cut(s) 487, 613
NspI RCATGY 1 cut(s) 40
PasI CCCWGGG 1 cut(s) 441
PcsI WCGNNNNNNNCGW 1 cut(s) 480
PfeI GAWTC 1 cut(s) 395
PflFI GACNNNGTC 1 cut(s) 299
PkrI GCNGC 4 cut(s) 242, 526, 588, 591
Psp6I CCWGG 3 cut(s) 440, 454, 604
PspGI CCWGG 3 cut(s) 440, 454, 604
PspN4I GGNNCC 1 cut(s) 70
PspPI GGNCC 1 cut(s) 301
PsyI GACNNNGTC 1 cut(s) 299
PvuII CAGCTG 1 cut(s) 589
RsaI GTAC 1 cut(s) 511
RsaNI GTAC 1 cut(s) 510
SaqAI TTAA 2 cut(s) 234, 630
SatI GCNGC 4 cut(s) 241, 525, 587, 590
Sau3AI GATC 3 cut(s) 466, 499, 643
Sau96I GGNCC 1 cut(s) 301
ScrFI CCNGG 3 cut(s) 442, 456, 606
SduI GDGCHC 2 cut(s) 186, 201
SetI ASST 8 cut(s) 127, 222, 303, 383, 460, 591, 610, 627
SexAI ACCWGGT 1 cut(s) 604
SfaNI GCATC 1 cut(s) 411
SfcI CTRYAG 1 cut(s) 579
SinI GGWCC 1 cut(s) 301
Sse9I AATT 4 cut(s) 148, 173, 519, 714
SsiI CCGC 2 cut(s) 17, 276
SspMI CTAG 4 cut(s) 378, 425, 557, 711
StyD4I CCNGG 3 cut(s) 440, 454, 604
StyI CCWWGG 1 cut(s) 377
TaaI ACNGT 3 cut(s) 359, 619, 700
TaiI ACGT 1 cut(s) 127
TaqI TCGA 2 cut(s) 474, 483
TasI AATT 4 cut(s) 148, 173, 519, 714
TfiI GAWTC 1 cut(s) 395
Tru1I TTAA 2 cut(s) 234, 630
Tru9I TTAA 2 cut(s) 234, 630
TscAI CASTG 2 cut(s) 364, 703
TseFI GTSAC 2 cut(s) 487, 613
TseI GCWGC 4 cut(s) 240, 524, 586, 589
Tsp45I GTSAC 2 cut(s) 487, 613
TspRI CASTG 2 cut(s) 364, 703
Tth111I GACNNNGTC 1 cut(s) 299
VpaK11BI GGWCC 1 cut(s) 301
XapI RAATTY 1 cut(s) 714
XbaI TCTAGA 1 cut(s) 556
XceI RCATGY 1 cut(s) 40
XmaJI CCTAGG 1 cut(s) 377
XspI CTAG 4 cut(s) 378, 425, 557, 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.